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The map label for this gene is emrB [H]

Identifier: 89256077

GI number: 89256077

Start: 675029

End: 676558

Strand: Reverse

Name: emrB [H]

Synonym: FTL_0688

Alternate gene names: 89256077

Gene position: 676558-675029 (Counterclockwise)

Preceding gene: 89256079

Following gene: 89256076

Centisome position: 35.68

GC content: 33.99

Gene sequence:

>1530_bases
ATGCAAGCTAGTAACACAATAACCGTTGGCTTTTGGAGCCAAAAAAGAACAATCGTAGCATTAGCAATAAGCTTACTGGC
ACTTGCTGAGATTGTTGACTTGACAATTGTCGCAGTTGCAATACCGCAGATTATGGGAGCAATCGGCGCTAATGTTGAAA
CCATAGCAGATGTAACTACAGTGTATATTGTCACTGCAGCAATTTTCATACTACTATCAGGTTTAATTATTGAGAAATAT
GGTATAAAAAGAGTAGCTCTAGTCTCATCAGTAATTTTTGGTATTTCATCAATAATGTGTGGACTTTCAACATCATTAGC
TGAAATGATTGTTTTTAGAGCTTTCCAAGGTATGGGAGGTGCTTTTTTACCATCGGTAGCACAGACGTATATATCTACCA
GCTTTAAAAATGAAGAGTATAACAAAATGATGACTATATATAGTATGGTTATCGTTATGGGACCAATTATTGGACCAGTA
CTTGGAGGCGCTATTTGTGAAAATATGTCTTGGGAATGGATTTTCTATGTCAATGTACCTCTTTGTATTGTGGCATTTAT
AATTATTGCATTTATGATGGAAGCAACACAAATCAAAAAAATCAAAATTGATTATATCAGTTTTGGTTTTATGGCAATAG
GGGTCGGCTGTTTAGAGCTTTTTATTGACAATGGTAATACCAATGGCTGGTTCTCATCGATTAAAATGATTATTCTTTTA
GCTATATTATTAGTTTCAATAGGATTTTTTATTTGGAGAGGTTTGATTTACTCTTCAGTAGTTAAATTTAAGATATTCAA
AAATTTTAACTTTGTCCTTGCATGCTTTTTGTGCTTTATGTTCGTATTATTATTCTCAGCTGCAATGGCATATTTACCAA
CTATGCTACAGCAAGTATATGGCTATCCTGTTGATCTAGCAGGATATATAACTGCTCCTAGAGGTGTTGCCGCTATTATT
GGTGCAATGCTAACACAATCTATTTTAGTAAAAAAATTAGGCGTAAGAAAAACTATAAGTCTCGGAATGATTACTTTTGG
GGTATCTTGCTTAATGCAAGCAAACTTTTCTCCTACCGCTAATGAATTTGATATCATACTAACAACGGCTATTCAGGGAT
TAGGAATGATGATGTTTTTTGTTCCTTTTATGAGTGTACTCGTAGTTGGTGTAGCTGATGAAGATATGGGCGATATGTCA
GGATCATTTAACTTTTTTAGAAACTTTGGTAGTTCTGTTGGTACTGCTTTTGTAGCAACATTTATTTCAAGAAATCAACA
AACCACCTATCTAGAATTATCACAAAATATATCATCACTAAATAACAACTTTCAAAGTTGGAGCCATAGCTTGCCTATGC
CTGATTTAGCTACAGTAGCTCTTGCAAAACAACAAGTATTACACCAAAGTAGCTTATTAAGCTATATTAACTCTTTTTAT
TTAGTTGGGATACTTAGTCTAATCTTAGCTATAGTACCATTTTTATTAAAAGAGCCACCTACAGGTGCGCCTGTTGCAGT
AATGCATTAA

Upstream 100 bases:

>100_bases
ATAATTTTACGCAAAAATAAACTCATTAAGTAATTTTCTTATTACTGCACAAAATGATGTAGTATGTTAGTGATATTTTA
ACCATAAAATTTTAAAACTT

Downstream 100 bases:

>100_bases
ATTATTATAGGATAATTAAAAATGTCAGAAGAAAAATTACAACAATCAACAATCACAAGAAATAAAAAAAGCTCGAGATT
TTCGCCTAAGAAAATTATCA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MQASNTITVGFWSQKRTIVALAISLLALAEIVDLTIVAVAIPQIMGAIGANVETIADVTTVYIVTAAIFILLSGLIIEKY
GIKRVALVSSVIFGISSIMCGLSTSLAEMIVFRAFQGMGGAFLPSVAQTYISTSFKNEEYNKMMTIYSMVIVMGPIIGPV
LGGAICENMSWEWIFYVNVPLCIVAFIIIAFMMEATQIKKIKIDYISFGFMAIGVGCLELFIDNGNTNGWFSSIKMIILL
AILLVSIGFFIWRGLIYSSVVKFKIFKNFNFVLACFLCFMFVLLFSAAMAYLPTMLQQVYGYPVDLAGYITAPRGVAAII
GAMLTQSILVKKLGVRKTISLGMITFGVSCLMQANFSPTANEFDIILTTAIQGLGMMMFFVPFMSVLVVGVADEDMGDMS
GSFNFFRNFGSSVGTAFVATFISRNQQTTYLELSQNISSLNNNFQSWSHSLPMPDLATVALAKQQVLHQSSLLSYINSFY
LVGILSLILAIVPFLLKEPPTGAPVAVMH

Sequences:

>Translated_509_residues
MQASNTITVGFWSQKRTIVALAISLLALAEIVDLTIVAVAIPQIMGAIGANVETIADVTTVYIVTAAIFILLSGLIIEKY
GIKRVALVSSVIFGISSIMCGLSTSLAEMIVFRAFQGMGGAFLPSVAQTYISTSFKNEEYNKMMTIYSMVIVMGPIIGPV
LGGAICENMSWEWIFYVNVPLCIVAFIIIAFMMEATQIKKIKIDYISFGFMAIGVGCLELFIDNGNTNGWFSSIKMIILL
AILLVSIGFFIWRGLIYSSVVKFKIFKNFNFVLACFLCFMFVLLFSAAMAYLPTMLQQVYGYPVDLAGYITAPRGVAAII
GAMLTQSILVKKLGVRKTISLGMITFGVSCLMQANFSPTANEFDIILTTAIQGLGMMMFFVPFMSVLVVGVADEDMGDMS
GSFNFFRNFGSSVGTAFVATFISRNQQTTYLELSQNISSLNNNFQSWSHSLPMPDLATVALAKQQVLHQSSLLSYINSFY
LVGILSLILAIVPFLLKEPPTGAPVAVMH
>Mature_509_residues
MQASNTITVGFWSQKRTIVALAISLLALAEIVDLTIVAVAIPQIMGAIGANVETIADVTTVYIVTAAIFILLSGLIIEKY
GIKRVALVSSVIFGISSIMCGLSTSLAEMIVFRAFQGMGGAFLPSVAQTYISTSFKNEEYNKMMTIYSMVIVMGPIIGPV
LGGAICENMSWEWIFYVNVPLCIVAFIIIAFMMEATQIKKIKIDYISFGFMAIGVGCLELFIDNGNTNGWFSSIKMIILL
AILLVSIGFFIWRGLIYSSVVKFKIFKNFNFVLACFLCFMFVLLFSAAMAYLPTMLQQVYGYPVDLAGYITAPRGVAAII
GAMLTQSILVKKLGVRKTISLGMITFGVSCLMQANFSPTANEFDIILTTAIQGLGMMMFFVPFMSVLVVGVADEDMGDMS
GSFNFFRNFGSSVGTAFVATFISRNQQTTYLELSQNISSLNNNFQSWSHSLPMPDLATVALAKQQVLHQSSLLSYINSFY
LVGILSLILAIVPFLLKEPPTGAPVAVMH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=29.5315682281059, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1788710, Length=489, Percent_Identity=29.8568507157464, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1790195, Length=427, Percent_Identity=25.9953161592506, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=25.8454106280193, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788392, Length=200, Percent_Identity=30.5, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788509, Length=174, Percent_Identity=25.2873563218391, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=181, Percent_Identity=31.4917127071823, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=29.5302013422819, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55496; Mature: 55496

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASNTITVGFWSQKRTIVALAISLLALAEIVDLTIVAVAIPQIMGAIGANVETIADVTT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VYIVTAAIFILLSGLIIEKYGIKRVALVSSVIFGISSIMCGLSTSLAEMIVFRAFQGMGG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AFLPSVAQTYISTSFKNEEYNKMMTIYSMVIVMGPIIGPVLGGAICENMSWEWIFYVNVP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCH
LCIVAFIIIAFMMEATQIKKIKIDYISFGFMAIGVGCLELFIDNGNTNGWFSSIKMIILL
HHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
AILLVSIGFFIWRGLIYSSVVKFKIFKNFNFVLACFLCFMFVLLFSAAMAYLPTMLQQVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYPVDLAGYITAPRGVAAIIGAMLTQSILVKKLGVRKTISLGMITFGVSCLMQANFSPTA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
NEFDIILTTAIQGLGMMMFFVPFMSVLVVGVADEDMGDMSGSFNFFRNFGSSVGTAFVAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCHHHHHHHHHH
FISRNQQTTYLELSQNISSLNNNFQSWSHSLPMPDLATVALAKQQVLHQSSLLSYINSFY
HHHCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVGILSLILAIVPFLLKEPPTGAPVAVMH
HHHHHHHHHHHHHHHHCCCCCCCCEECCC
>Mature Secondary Structure
MQASNTITVGFWSQKRTIVALAISLLALAEIVDLTIVAVAIPQIMGAIGANVETIADVTT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VYIVTAAIFILLSGLIIEKYGIKRVALVSSVIFGISSIMCGLSTSLAEMIVFRAFQGMGG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AFLPSVAQTYISTSFKNEEYNKMMTIYSMVIVMGPIIGPVLGGAICENMSWEWIFYVNVP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCH
LCIVAFIIIAFMMEATQIKKIKIDYISFGFMAIGVGCLELFIDNGNTNGWFSSIKMIILL
HHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
AILLVSIGFFIWRGLIYSSVVKFKIFKNFNFVLACFLCFMFVLLFSAAMAYLPTMLQQVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYPVDLAGYITAPRGVAAIIGAMLTQSILVKKLGVRKTISLGMITFGVSCLMQANFSPTA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
NEFDIILTTAIQGLGMMMFFVPFMSVLVVGVADEDMGDMSGSFNFFRNFGSSVGTAFVAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCHHHHHHHHHH
FISRNQQTTYLELSQNISSLNNNFQSWSHSLPMPDLATVALAKQQVLHQSSLLSYINSFY
HHHCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVGILSLILAIVPFLLKEPPTGAPVAVMH
HHHHHHHHHHHHHHHHCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]