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The map label for this gene is nadE [H]

Identifier: 89256074

GI number: 89256074

Start: 671625

End: 672374

Strand: Reverse

Name: nadE [H]

Synonym: FTL_0685

Alternate gene names: 89256074

Gene position: 672374-671625 (Counterclockwise)

Preceding gene: 89256075

Following gene: 89256073

Centisome position: 35.46

GC content: 34.53

Gene sequence:

>750_bases
ATGAAAATAGTTAAAGATTTTAGTCCTAAAGAATATTCACAAAAGTTAGTTAATTGGCTAAGTGATAGTTGTATGAATTA
TCCCGCTGAAGGATTTGTGATTGGTCTTAGTGGCGGTATAGATTCAGCAGTTGCGGCTTCTTTAGCTGTCAAAACTGGAT
TACCAACTACAGCTTTAATACTACCTTCAGATAATAATCAACACCAAGATATGCAAGATGCTCTAGAACTTATTGAAATG
CTTAATATTGAACATTATACCATTTCGATTCAACCAGCTTATGAGGCTTTTCTTGCTTCAACGCAAAGCTTTACAAATCT
ACAAAACAATAGACAACTTGTGATCAAGGGAAATGCTCAAGCACGTTTAAGGATGATGTATTTGTATGCCTATGCGCAAC
AATATAACAGAATAGTTATAGGTACTGATAATGCTTGTGAGTGGTATATGGGATATTTTACAAAATTCGGTGATGGGGCT
GCCGATATACTTCCACTAGTTAATCTCAAAAAATCTCAAGTTTTTGAATTAGGCAAATACCTAGATGTCCCTAAAAACAT
ACTTGATAAAGCTCCATCTGCAGGACTATGGCAAGGACAAACTGATGAGGATGAAATGGGTGTAACTTATCAAGAAATTG
ATGATTTCTTAGATGGTAAACAAGTTTCAGCAAAAGCTCTAGAAAGAATAAATTTCTGGCATAATCGTAGTCACCATAAG
AGAAAATTAGCTTTAACTCCTAATTTCTGA

Upstream 100 bases:

>100_bases
CTCTAAAAAAATATTCTAAAATAAGCTCATGATTATGATAAAATCAGCTCCTAAAACTGATTTATCTTAAAAAAATAATT
TTTATTAACAAGGTTAAAAA

Downstream 100 bases:

>100_bases
ATATACTTAAGTGATTTTCTTCTACAAAAACAAACATAGATATTGATTAGTTGTTTTCTTGCAGTAAAATAATCAAAAAA
TAACCACCTATTAAAGTCTA

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKIVKDFSPKEYSQKLVNWLSDSCMNYPAEGFVIGLSGGIDSAVAASLAVKTGLPTTALILPSDNNQHQDMQDALELIEM
LNIEHYTISIQPAYEAFLASTQSFTNLQNNRQLVIKGNAQARLRMMYLYAYAQQYNRIVIGTDNACEWYMGYFTKFGDGA
ADILPLVNLKKSQVFELGKYLDVPKNILDKAPSAGLWQGQTDEDEMGVTYQEIDDFLDGKQVSAKALERINFWHNRSHHK
RKLALTPNF

Sequences:

>Translated_249_residues
MKIVKDFSPKEYSQKLVNWLSDSCMNYPAEGFVIGLSGGIDSAVAASLAVKTGLPTTALILPSDNNQHQDMQDALELIEM
LNIEHYTISIQPAYEAFLASTQSFTNLQNNRQLVIKGNAQARLRMMYLYAYAQQYNRIVIGTDNACEWYMGYFTKFGDGA
ADILPLVNLKKSQVFELGKYLDVPKNILDKAPSAGLWQGQTDEDEMGVTYQEIDDFLDGKQVSAKALERINFWHNRSHHK
RKLALTPNF
>Mature_249_residues
MKIVKDFSPKEYSQKLVNWLSDSCMNYPAEGFVIGLSGGIDSAVAASLAVKTGLPTTALILPSDNNQHQDMQDALELIEM
LNIEHYTISIQPAYEAFLASTQSFTNLQNNRQLVIKGNAQARLRMMYLYAYAQQYNRIVIGTDNACEWYMGYFTKFGDGA
ADILPLVNLKKSQVFELGKYLDVPKNILDKAPSAGLWQGQTDEDEMGVTYQEIDDFLDGKQVSAKALERINFWHNRSHHK
RKLALTPNF

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=253, Percent_Identity=34.7826086956522, Blast_Score=126, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24641841, Length=264, Percent_Identity=28.030303030303, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641843, Length=264, Percent_Identity=28.030303030303, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 28086; Mature: 28086

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVKDFSPKEYSQKLVNWLSDSCMNYPAEGFVIGLSGGIDSAVAASLAVKTGLPTTALI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEE
LPSDNNQHQDMQDALELIEMLNIEHYTISIQPAYEAFLASTQSFTNLQNNRQLVIKGNAQ
EECCCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCC
ARLRMMYLYAYAQQYNRIVIGTDNACEWYMGYFTKFGDGAADILPLVNLKKSQVFELGKY
HHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
LDVPKNILDKAPSAGLWQGQTDEDEMGVTYQEIDDFLDGKQVSAKALERINFWHNRSHHK
HCCCHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
RKLALTPNF
EEEEECCCC
>Mature Secondary Structure
MKIVKDFSPKEYSQKLVNWLSDSCMNYPAEGFVIGLSGGIDSAVAASLAVKTGLPTTALI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEE
LPSDNNQHQDMQDALELIEMLNIEHYTISIQPAYEAFLASTQSFTNLQNNRQLVIKGNAQ
EECCCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCC
ARLRMMYLYAYAQQYNRIVIGTDNACEWYMGYFTKFGDGAADILPLVNLKKSQVFELGKY
HHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
LDVPKNILDKAPSAGLWQGQTDEDEMGVTYQEIDDFLDGKQVSAKALERINFWHNRSHHK
HCCCHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
RKLALTPNF
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA