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The map label for this gene is metH [H]

Identifier: 89054467

GI number: 89054467

Start: 1970534

End: 1971604

Strand: Reverse

Name: metH [H]

Synonym: Jann_1976

Alternate gene names: 89054467

Gene position: 1971604-1970534 (Counterclockwise)

Preceding gene: 89054468

Following gene: 89054466

Centisome position: 45.66

GC content: 64.05

Gene sequence:

>1071_bases
GTGACGCGCACTGTTATCGAGTCGAAGACCAAGACCACCGTGATCGGCTTTGACGAGCCGTTTTGCGTCATCGGGGAGCG
GATCAACCCCACCGGGCGCAAGATCCTGAACCAGGAGTTGGAGGACGGCGATTTCAGCCGCGTGGAGGCGGACGCGATTG
CGCAGGTCGCGGCGGGGGCGACGGTGCTGGACATCAACTCGGGCGCGGTGTTTTCGGGCAAGATGGCCGAGGATCCGCGC
TATGCGGACAACAACTTTGTCGAACCGCCGTTGATGAAGCAGTTGATCGAGGTTGTGCAGGCGGTAACCGATTGCCCGCT
GTGCATCGACAGCTCCGTCCCCGGGGCGCTGGAGCAGGGGCTGGCGGCGGCCGAGGGGCGACCGCTCCTGAACTCCGTCA
CCGGGGAAGAAGAGCGGCTGGAACTGGTCCTGCCACTGGTCGCCAAATACAACGTGCCCGTGGTGGCGATTTCCAACGAC
GACACCGGGATCAGTGAAGACCCCGATGTGCGCTTTGCGGTCGCCAAGAAGATCGTGGAGCGGGCGGCGGATTTCGGCAT
CCCCGCCCATGACATCGTTGTCGATCCGCTGGTCATGCCCATCGGCGCCATGGCCACCGCAGGCCACCAGGTCTTCACCC
TCGTGCGCCGCCTGCGCGATGAATTGGGCGTGAACACCACCTGTGGCGCGTCGAATATTTCCTTCGGGCTGCCCAATCGG
CACGGCATCAACAATGCCTTCCTGCCCATGGCGTTCAATGCGGGCATGACGTCGGCGATCATGAACCCCGTGGCCCTTCC
CGTTGGCCCCAAGAAGATCGCCGAGAAGAAGGCGCTGGTGGAAGCCGCCGGGGTGATCCTGCCCGAGGGCATGGATGATG
AGGCTTTTGTGAAGCTCTTCGGCATGGGATCGACCAAACCCCGTGCAGGCAAGGAAATGGAGGCCATTCGCGCTGCCAAC
TTCCTGCTCAACCACGACGACGGCGGTGGGGCCTGGATCAATTTCAACAAGGATCCCGATGCCGCCCCGCGCCGGGGCCG
CGAAGGTGGGCGCAGGCGTGGGCGCGGGTGA

Upstream 100 bases:

>100_bases
TGACGTCGCCTCTGCCCGTGTCTATGTCGCCTCTGTGCCGTCCCTCGCCACCTGCCCCCCCTAGGCTCGGGCGAAACGGC
CCATCAAGGAGAGCCCGCCC

Downstream 100 bases:

>100_bases
TCGCGCCCCTTCCCCTCCCTCCAAAGGCACTCCGATCCGGGTCGGGGTGCCTTTCGCCATGACAGAGCCCGCATCGTCCA
ACTGGATCTGGCCCGCCCTG

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRTVIESKTKTTVIGFDEPFCVIGERINPTGRKILNQELEDGDFSRVEADAIAQVAAGATVLDINSGAVFSGKMAEDPR
YADNNFVEPPLMKQLIEVVQAVTDCPLCIDSSVPGALEQGLAAAEGRPLLNSVTGEEERLELVLPLVAKYNVPVVAISND
DTGISEDPDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLRDELGVNTTCGASNISFGLPNR
HGINNAFLPMAFNAGMTSAIMNPVALPVGPKKIAEKKALVEAAGVILPEGMDDEAFVKLFGMGSTKPRAGKEMEAIRAAN
FLLNHDDGGGAWINFNKDPDAAPRRGREGGRRRGRG

Sequences:

>Translated_356_residues
MTRTVIESKTKTTVIGFDEPFCVIGERINPTGRKILNQELEDGDFSRVEADAIAQVAAGATVLDINSGAVFSGKMAEDPR
YADNNFVEPPLMKQLIEVVQAVTDCPLCIDSSVPGALEQGLAAAEGRPLLNSVTGEEERLELVLPLVAKYNVPVVAISND
DTGISEDPDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLRDELGVNTTCGASNISFGLPNR
HGINNAFLPMAFNAGMTSAIMNPVALPVGPKKIAEKKALVEAAGVILPEGMDDEAFVKLFGMGSTKPRAGKEMEAIRAAN
FLLNHDDGGGAWINFNKDPDAAPRRGREGGRRRGRG
>Mature_355_residues
TRTVIESKTKTTVIGFDEPFCVIGERINPTGRKILNQELEDGDFSRVEADAIAQVAAGATVLDINSGAVFSGKMAEDPRY
ADNNFVEPPLMKQLIEVVQAVTDCPLCIDSSVPGALEQGLAAAEGRPLLNSVTGEEERLELVLPLVAKYNVPVVAISNDD
TGISEDPDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLRDELGVNTTCGASNISFGLPNRH
GINNAFLPMAFNAGMTSAIMNPVALPVGPKKIAEKKALVEAAGVILPEGMDDEAFVKLFGMGSTKPRAGKEMEAIRAANF
LLNHDDGGGAWINFNKDPDAAPRRGREGGRRRGRG

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=311, Percent_Identity=26.3665594855305, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790450, Length=283, Percent_Identity=25.4416961130742, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=268, Percent_Identity=26.865671641791, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37954; Mature: 37823

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTVIESKTKTTVIGFDEPFCVIGERINPTGRKILNQELEDGDFSRVEADAIAQVAAGA
CCCCCCCCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
TVLDINSGAVFSGKMAEDPRYADNNFVEPPLMKQLIEVVQAVTDCPLCIDSSVPGALEQG
EEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHC
LAAAEGRPLLNSVTGEEERLELVLPLVAKYNVPVVAISNDDTGISEDPDVRFAVAKKIVE
HHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
RAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLRDELGVNTTCGASNISFGLPNR
HHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HGINNAFLPMAFNAGMTSAIMNPVALPVGPKKIAEKKALVEAAGVILPEGMDDEAFVKLF
CCCCCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCHHHHHEEE
GMGSTKPRAGKEMEAIRAANFLLNHDDGGGAWINFNKDPDAAPRRGREGGRRRGRG
ECCCCCCCCCHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRTVIESKTKTTVIGFDEPFCVIGERINPTGRKILNQELEDGDFSRVEADAIAQVAAGA
CCCCCCCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
TVLDINSGAVFSGKMAEDPRYADNNFVEPPLMKQLIEVVQAVTDCPLCIDSSVPGALEQG
EEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHC
LAAAEGRPLLNSVTGEEERLELVLPLVAKYNVPVVAISNDDTGISEDPDVRFAVAKKIVE
HHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
RAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLRDELGVNTTCGASNISFGLPNR
HHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HGINNAFLPMAFNAGMTSAIMNPVALPVGPKKIAEKKALVEAAGVILPEGMDDEAFVKLF
CCCCCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCHHHHHEEE
GMGSTKPRAGKEMEAIRAANFLLNHDDGGGAWINFNKDPDAAPRRGREGGRRRGRG
ECCCCCCCCCHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]