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The map label for this gene is ccsA [H]

Identifier: 89054332

GI number: 89054332

Start: 1826195

End: 1827487

Strand: Reverse

Name: ccsA [H]

Synonym: Jann_1841

Alternate gene names: 89054332

Gene position: 1827487-1826195 (Counterclockwise)

Preceding gene: 89054333

Following gene: 89054330

Centisome position: 42.32

GC content: 59.71

Gene sequence:

>1293_bases
ATGGCCGACACCAGAACCGCCACTTTGACCGTTGACGGACAGACCGTCGATCTGCCCATTCATTCGCCCACCGCAGGCCC
CGACGTCGTCGACATCGGCAAGCTCTATGGGCAGGCCGGTGTGTTCACCCATGACCCGGGCTTCACCTCCACCTCGGCGT
GCGAATCGTCGATCACGTTCATTGATGGTGACAAGGGCGAGTTGCTGCATCGCGGCTACCCCATCGACCAACTGGCAAGC
CAGTCCAACTACATCGAAGTCTGCTTCCTGCTGCTTTATGGCTATCTGCCCTCGCGCGCGGAATACGACAAGTTCGAGAC
GACCATTACCCACCACACCATGCTGCACGAGCAGATGGCTAACTTCTTCCGGGGTTTCCGCCGGGACGCCCATCCCATGG
CGGTCATGGTCGGCGTCGTGGGCGCAATGTCTGCGTTCTATCACGACAGCACCGATATCCATGATGAGCATCAGCGCGAG
GTCGCCTCGCACCGCCTGATCGCCAAGATGCCGACGATTGCGGCCTGGGCCTATAAATACACCATCGGCCAGCCCTTCGT
GTATCCGCGCAATGACATGAGCTACGCGGCCAACTTCCTGCGCATGTGCTTTGCGGTTCCGGCGGAGGAATACGTGGTCG
ACCCGATCCTTGAGAAGGCGATGGACCGCATCTTCACGCTCCATGCGGATCACGAACAAAACGCGTCCACGTCGACCGTG
CGTTTGGCCTCATCCTCCGGCGCCAATCCCTTCGCCTGCATCGCCGCCGGGATTGCCTGCCTCTGGGGTCCGGCCCATGG
CGGCGCGAACCAGGCCTGTCTGGAGATGCTGAAGGAAATCGGCACGGTTGACCGCATTCCAGAGTTTATCGCGCGGGCCA
AGGACAAGAACGACAGCTACCGCCTGATGGGCTTTGGCCACCGGGTCTACAAGAACTTCGATCCCCGTGCGACGGTGATG
AAACAATCCGCCGACGAAGTGCTGGAGTTGCTTGGGGTTGAGGACAATCCGCTTCTGCAGGTCGCGATGGAGCTGGAAAA
ACAGGCGCTGGCTGATCCCTACTTCTCCGAGAAGAAGCTGTTCCCCAACGTCGATTTCTACTCCGGCATTATTCTAGAGG
CGATGGGCTTTCCGACGTCAATGTTCACACCCATCTTCGCGCTGTCGCGCACCGTGGGCTGGATTTCCCAATGGAAAGAA
CAGCTTGGCGACCCAGCCCTGAAAATTGGCCGCCCCCGCCAGCTTTATACTGGCGAGACGGCGCGCGATTACGTGGACAT
CAAAGACCGCTGA

Upstream 100 bases:

>100_bases
CTCCCTCACCTGACAGCTGGGGGGCAGCACCTGAGGGGGCTTCCCCCTCCCCCACATTTCCGCCGGCATCCCGCCGCGCG
CGCCTCACGAAGGGACGACC

Downstream 100 bases:

>100_bases
CGCCACCGCCCCGGACGCACAAAAGGCCGCTCCCACCAAAGGGGCGGCCTTTCCTTTGCCTAGCCGACCCGCACGCGCAA
TCTGAACCGTTCCGTGCTTC

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MADTRTATLTVDGQTVDLPIHSPTAGPDVVDIGKLYGQAGVFTHDPGFTSTSACESSITFIDGDKGELLHRGYPIDQLAS
QSNYIEVCFLLLYGYLPSRAEYDKFETTITHHTMLHEQMANFFRGFRRDAHPMAVMVGVVGAMSAFYHDSTDIHDEHQRE
VASHRLIAKMPTIAAWAYKYTIGQPFVYPRNDMSYAANFLRMCFAVPAEEYVVDPILEKAMDRIFTLHADHEQNASTSTV
RLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTVDRIPEFIARAKDKNDSYRLMGFGHRVYKNFDPRATVM
KQSADEVLELLGVEDNPLLQVAMELEKQALADPYFSEKKLFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKE
QLGDPALKIGRPRQLYTGETARDYVDIKDR

Sequences:

>Translated_430_residues
MADTRTATLTVDGQTVDLPIHSPTAGPDVVDIGKLYGQAGVFTHDPGFTSTSACESSITFIDGDKGELLHRGYPIDQLAS
QSNYIEVCFLLLYGYLPSRAEYDKFETTITHHTMLHEQMANFFRGFRRDAHPMAVMVGVVGAMSAFYHDSTDIHDEHQRE
VASHRLIAKMPTIAAWAYKYTIGQPFVYPRNDMSYAANFLRMCFAVPAEEYVVDPILEKAMDRIFTLHADHEQNASTSTV
RLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTVDRIPEFIARAKDKNDSYRLMGFGHRVYKNFDPRATVM
KQSADEVLELLGVEDNPLLQVAMELEKQALADPYFSEKKLFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKE
QLGDPALKIGRPRQLYTGETARDYVDIKDR
>Mature_429_residues
ADTRTATLTVDGQTVDLPIHSPTAGPDVVDIGKLYGQAGVFTHDPGFTSTSACESSITFIDGDKGELLHRGYPIDQLASQ
SNYIEVCFLLLYGYLPSRAEYDKFETTITHHTMLHEQMANFFRGFRRDAHPMAVMVGVVGAMSAFYHDSTDIHDEHQREV
ASHRLIAKMPTIAAWAYKYTIGQPFVYPRNDMSYAANFLRMCFAVPAEEYVVDPILEKAMDRIFTLHADHEQNASTSTVR
LASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTVDRIPEFIARAKDKNDSYRLMGFGHRVYKNFDPRATVMK
QSADEVLELLGVEDNPLLQVAMELEKQALADPYFSEKKLFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKEQ
LGDPALKIGRPRQLYTGETARDYVDIKDR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=377, Percent_Identity=25.7294429708223, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=67.4418604651163, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI1786527, Length=385, Percent_Identity=29.8701298701299, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=425, Percent_Identity=26.1176470588235, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319850, Length=363, Percent_Identity=28.099173553719, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=349, Percent_Identity=25.5014326647564, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=369, Percent_Identity=26.0162601626016, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640126, Length=407, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24640124, Length=407, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356863, Length=436, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47968; Mature: 47837

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTRTATLTVDGQTVDLPIHSPTAGPDVVDIGKLYGQAGVFTHDPGFTSTSACESSITF
CCCCEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHCCCCEE
IDGDKGELLHRGYPIDQLASQSNYIEVCFLLLYGYLPSRAEYDKFETTITHHTMLHEQMA
EECCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
NFFRGFRRDAHPMAVMVGVVGAMSAFYHDSTDIHDEHQREVASHRLIAKMPTIAAWAYKY
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHEEE
TIGQPFVYPRNDMSYAANFLRMCFAVPAEEYVVDPILEKAMDRIFTLHADHEQNASTSTV
ECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEE
RLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTVDRIPEFIARAKDKNDSY
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCE
RLMGFGHRVYKNFDPRATVMKQSADEVLELLGVEDNPLLQVAMELEKQALADPYFSEKKL
EEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKEQLGDPALKIGRPRQLYTGET
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
ARDYVDIKDR
HHCCCCCCCC
>Mature Secondary Structure 
ADTRTATLTVDGQTVDLPIHSPTAGPDVVDIGKLYGQAGVFTHDPGFTSTSACESSITF
CCCEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHCCCCEE
IDGDKGELLHRGYPIDQLASQSNYIEVCFLLLYGYLPSRAEYDKFETTITHHTMLHEQMA
EECCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
NFFRGFRRDAHPMAVMVGVVGAMSAFYHDSTDIHDEHQREVASHRLIAKMPTIAAWAYKY
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHEEE
TIGQPFVYPRNDMSYAANFLRMCFAVPAEEYVVDPILEKAMDRIFTLHADHEQNASTSTV
ECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEE
RLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGTVDRIPEFIARAKDKNDSY
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCE
RLMGFGHRVYKNFDPRATVMKQSADEVLELLGVEDNPLLQVAMELEKQALADPYFSEKKL
EEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKEQLGDPALKIGRPRQLYTGET
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
ARDYVDIKDR
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]