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The map label for this gene is gltX

Identifier: 89054333

GI number: 89054333

Start: 1827575

End: 1828987

Strand: Reverse

Name: gltX

Synonym: Jann_1842

Alternate gene names: 89054333

Gene position: 1828987-1827575 (Counterclockwise)

Preceding gene: 89054340

Following gene: 89054332

Centisome position: 42.36

GC content: 63.34

Gene sequence:

>1413_bases
ATGAGTTCAGACGCCGCAAGCCCAGTCGTCACCCGCATCGCGCCCTCACCCACTGGAACCATGCATATCGGCACCGCGCG
CACGGGCCTGTTCAACTGGTTATTTGCCCGCAAGCATGGCGGTAAATTCGTGCTGCGGATTGAGGATACGGACCGAGAGC
GCTCCACCCCCGAAGCGACGCAGGCCATTCTGGACGGCATGGCGTGGCTTGGCCTGGACTACGATGGCGAGGCCGTGAGC
CAGTTCGCCCGCGCGGATCGGCACCGGGAGGTGGCGGAGCAGATGCTGGCAGAGGGCCATGCCTACAAGTGTTTCTCCAC
CCAGGACGAGATCGCGGCGTTCCGGGACGCGGCCAAGGCAGACGGACGCTCCACCCTATTCCTGTCGCCATGGCGCGAAG
CGAGCGACCACCCGGACGCACCATACGTGATCCGCCTCAAAGCCCCGCGCGACGGCACGACCGTGATCCGCGACCGTGTG
CAGAGCGACGTGACGATCCAGAACGCGACGTTGGACGACATGATCCTGCTGCGGTCCGACGGGACGCCGACCTATATGCA
CGCGGTCGTTGTCGATGATCACGACATGGGCGTGACCCATGTGGTCCGGGGCGATGACCACCTCAACAACGCCGCGCGCC
AGATCCAGATCTACGCCGCGATGGGATGGGCCGAGCCGGTTTGGTCCCACATCCCGCTGATCCATGGCGATGACGGCAAG
AAGCTGTCCAAGCGCCACGGGGCCACGGGCGTGATGGAATATGCCGAGATGGGCATCCCCGCGGCGGCGATGCGCAACTA
CCTGACGCGGCTTGGCTGGTCCCACGGGGATGAAGAGCTGTTTACCGACGAACAGGCCCGCGACTGGTTTGATCTCGACG
GAATCGGCAAATCGCCCTCGCGGCTGGACATGAAGAAACTGGCCAATGTTACGGGCTGGCACATCGCCCGGACAGACGAC
GCGGATCTGGTGGCACAGATCGAAGGCTATCTGGCGCTGACCGGTGCGCCCGCCCTTTCGCAGGCGCAGCATGACGGGCT
GACCCGTGCGATGTATTGCCTCAAGGATCGCGCGAAGTCCTTGCCGGACTTGTTGGATAAGGGGCGGTTTGTGTTGGAAA
ATCGCCCGTTCGACTATGATGAAAAGTCCGTGACCATGCTCGATCCGGTATCCCGTAGTATACTGCGGGAATTGACCGCG
CAGTTGCAGAATGCTAGGTGGACCCGAGACACGCTAGAGGGCGTTCTGAACGCCGCGGCAGACTCCCATGGGTTGAAATT
CGGCAAACTGGCCGGACCGCTGCGCGCGGCCCTCTCCGGGCGATCCGTGAGCCCAAGCGTGTTTGACATGATGCTTGTCA
TCGGCCGGGACGAAACACTCGCCCGCCTTGATGAGGCCGCCTCCCTCACCTGA

Upstream 100 bases:

>100_bases
GTCCCTTGCCCAAGATACCTAGCCTTGAAGATGCCAGATCATGGTTTCCAAAAGGTTAACGCGCCAGTCGTTTGGCAAGG
CGCTGTAAAGGATGTCCCGC

Downstream 100 bases:

>100_bases
CAGCTGGGGGGCAGCACCTGAGGGGGCTTCCCCCTCCCCCACATTTCCGCCGGCATCCCGCCGCGCGCGCCTCACGAAGG
GACGACCATGGCCGACACCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSSDAASPVVTRIAPSPTGTMHIGTARTGLFNWLFARKHGGKFVLRIEDTDRERSTPEATQAILDGMAWLGLDYDGEAVS
QFARADRHREVAEQMLAEGHAYKCFSTQDEIAAFRDAAKADGRSTLFLSPWREASDHPDAPYVIRLKAPRDGTTVIRDRV
QSDVTIQNATLDDMILLRSDGTPTYMHAVVVDDHDMGVTHVVRGDDHLNNAARQIQIYAAMGWAEPVWSHIPLIHGDDGK
KLSKRHGATGVMEYAEMGIPAAAMRNYLTRLGWSHGDEELFTDEQARDWFDLDGIGKSPSRLDMKKLANVTGWHIARTDD
ADLVAQIEGYLALTGAPALSQAQHDGLTRAMYCLKDRAKSLPDLLDKGRFVLENRPFDYDEKSVTMLDPVSRSILRELTA
QLQNARWTRDTLEGVLNAAADSHGLKFGKLAGPLRAALSGRSVSPSVFDMMLVIGRDETLARLDEAASLT

Sequences:

>Translated_470_residues
MSSDAASPVVTRIAPSPTGTMHIGTARTGLFNWLFARKHGGKFVLRIEDTDRERSTPEATQAILDGMAWLGLDYDGEAVS
QFARADRHREVAEQMLAEGHAYKCFSTQDEIAAFRDAAKADGRSTLFLSPWREASDHPDAPYVIRLKAPRDGTTVIRDRV
QSDVTIQNATLDDMILLRSDGTPTYMHAVVVDDHDMGVTHVVRGDDHLNNAARQIQIYAAMGWAEPVWSHIPLIHGDDGK
KLSKRHGATGVMEYAEMGIPAAAMRNYLTRLGWSHGDEELFTDEQARDWFDLDGIGKSPSRLDMKKLANVTGWHIARTDD
ADLVAQIEGYLALTGAPALSQAQHDGLTRAMYCLKDRAKSLPDLLDKGRFVLENRPFDYDEKSVTMLDPVSRSILRELTA
QLQNARWTRDTLEGVLNAAADSHGLKFGKLAGPLRAALSGRSVSPSVFDMMLVIGRDETLARLDEAASLT
>Mature_469_residues
SSDAASPVVTRIAPSPTGTMHIGTARTGLFNWLFARKHGGKFVLRIEDTDRERSTPEATQAILDGMAWLGLDYDGEAVSQ
FARADRHREVAEQMLAEGHAYKCFSTQDEIAAFRDAAKADGRSTLFLSPWREASDHPDAPYVIRLKAPRDGTTVIRDRVQ
SDVTIQNATLDDMILLRSDGTPTYMHAVVVDDHDMGVTHVVRGDDHLNNAARQIQIYAAMGWAEPVWSHIPLIHGDDGKK
LSKRHGATGVMEYAEMGIPAAAMRNYLTRLGWSHGDEELFTDEQARDWFDLDGIGKSPSRLDMKKLANVTGWHIARTDDA
DLVAQIEGYLALTGAPALSQAQHDGLTRAMYCLKDRAKSLPDLLDKGRFVLENRPFDYDEKSVTMLDPVSRSILRELTAQ
LQNARWTRDTLEGVLNAAADSHGLKFGKLAGPLRAALSGRSVSPSVFDMMLVIGRDETLARLDEAASLT

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=504, Percent_Identity=29.1666666666667, Blast_Score=196, Evalue=6e-50,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=40.301724137931, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI226510925, Length=244, Percent_Identity=34.8360655737705, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=28.4536082474227, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324540, Length=322, Percent_Identity=33.8509316770186, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324742, Length=119, Percent_Identity=32.7731092436975, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=26.2008733624454, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=504, Percent_Identity=30.952380952381, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_JANSC (Q28RA3)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509784.1
- ProteinModelPortal:   Q28RA3
- SMR:   Q28RA3
- STRING:   Q28RA3
- GeneID:   3934293
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1842
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   DAQAREW
- PhylomeDB:   Q28RA3
- ProtClustDB:   PRK01406
- BioCyc:   JSP290400:JANN_1842-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51879; Mature: 51748

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDAASPVVTRIAPSPTGTMHIGTARTGLFNWLFARKHGGKFVLRIEDTDRERSTPEAT
CCCCCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
QAILDGMAWLGLDYDGEAVSQFARADRHREVAEQMLAEGHAYKCFSTQDEIAAFRDAAKA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHC
DGRSTLFLSPWREASDHPDAPYVIRLKAPRDGTTVIRDRVQSDVTIQNATLDDMILLRSD
CCCEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEEEECC
GTPTYMHAVVVDDHDMGVTHVVRGDDHLNNAARQIQIYAAMGWAEPVWSHIPLIHGDDGK
CCCEEEEEEEEECCCCCEEEEECCCHHHCCHHHEEEEEEECCCCCHHHHHCCEEECCCCC
KLSKRHGATGVMEYAEMGIPAAAMRNYLTRLGWSHGDEELFTDEQARDWFDLDGIGKSPS
HHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHCCHHHCCCCCCCCCCCCCC
RLDMKKLANVTGWHIARTDDADLVAQIEGYLALTGAPALSQAQHDGLTRAMYCLKDRAKS
HHHHHHHCCCCCCEEEECCCHHHHHHHCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHH
LPDLLDKGRFVLENRPFDYDEKSVTMLDPVSRSILRELTAQLQNARWTRDTLEGVLNAAA
HHHHHHCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DSHGLKFGKLAGPLRAALSGRSVSPSVFDMMLVIGRDETLARLDEAASLT
HHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SSDAASPVVTRIAPSPTGTMHIGTARTGLFNWLFARKHGGKFVLRIEDTDRERSTPEAT
CCCCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
QAILDGMAWLGLDYDGEAVSQFARADRHREVAEQMLAEGHAYKCFSTQDEIAAFRDAAKA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHC
DGRSTLFLSPWREASDHPDAPYVIRLKAPRDGTTVIRDRVQSDVTIQNATLDDMILLRSD
CCCEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEEEECC
GTPTYMHAVVVDDHDMGVTHVVRGDDHLNNAARQIQIYAAMGWAEPVWSHIPLIHGDDGK
CCCEEEEEEEEECCCCCEEEEECCCHHHCCHHHEEEEEEECCCCCHHHHHCCEEECCCCC
KLSKRHGATGVMEYAEMGIPAAAMRNYLTRLGWSHGDEELFTDEQARDWFDLDGIGKSPS
HHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHCCHHHCCCCCCCCCCCCCC
RLDMKKLANVTGWHIARTDDADLVAQIEGYLALTGAPALSQAQHDGLTRAMYCLKDRAKS
HHHHHHHCCCCCCEEEECCCHHHHHHHCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHH
LPDLLDKGRFVLENRPFDYDEKSVTMLDPVSRSILRELTAQLQNARWTRDTLEGVLNAAA
HHHHHHCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DSHGLKFGKLAGPLRAALSGRSVSPSVFDMMLVIGRDETLARLDEAASLT
HHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA