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The map label for this gene is ksgA

Identifier: 89054300

GI number: 89054300

Start: 1797474

End: 1798391

Strand: Reverse

Name: ksgA

Synonym: Jann_1809

Alternate gene names: 89054300

Gene position: 1798391-1797474 (Counterclockwise)

Preceding gene: 89054301

Following gene: 89054295

Centisome position: 41.65

GC content: 67.1

Gene sequence:

>918_bases
ATGCCCCATCCCCCCGAGAGGTGTATGGCCAAGATGAAGGAGCGGCGCATGGTGGCCATTGACGGGCTTCCCCCGCTGCG
GGACGTCATTGCGGCCCACGGGTTGTCGGCGCGAAAGGCGCTGGGGCAGAACTTTCTGCTGGACCTGAACCTGACGGCGA
AGATTGCGCGGCTGGCTGGGGATTTGACCAGCGTCGATGTGTTAGAAGTGGGCCCCGGCCCCGGTGGCCTGACCCGTGGG
TTATTGGCAGAGGGCGCACGCCGGGTGGTGGCCGTGGAGAAAGACCCCCGTTGCCTGCCCGTGCTGGCGGAGATTGAGGC
CATCTATCCCGGCCGTCTCAAGGTTTTGAACGCCGACGCGTTGGAGCTGGATTGGGCGGCGGACCTGCAAGCCCCCCGCA
AGATCGTCGCCAATCTGCCCTACAATGTGGGTACGGAATTGCTTGTCCGCTGGCTCACCCCCGCGTCCTGGCCGCCCCCG
TGGGAGAGCCTGACCCTGATGTTCCAGCGCGAAGTGGCGGAGCGGATCGTGGCGCAGCCCGGCTCCAAAACCTACGGACG
CCTTGCGATCCTGTCGCAATGGCGGGCCGATCCACGGATCGTGATGGGCCTGCCGCCGGAGGCGTTCACCCCGCCGCCCA
AGGTCCATTCGGCGGTGGTCCACTTTACCGCCCTGCCTGCGCCCCGGTTCCCGGCGGACGCCCGCGTTCTGACCCGCGTT
GTGGCTGCTGCGTTCGGGCAGCGCCGCAAGATGTTGCGCGCCGCGTTGAAGGGCTTGGCCCCGGATATTGAGGATCGTCT
GGTTGCCGCCGGACTAAAACCCACGGATCGCGCGGAGCAGGTGCCGCTGGAGGGCTTCTGCGCCCTCGCCCGCGTCATGG
AGGATGTCATCACGCCAAGCCACGGGCGCGAGGCATAA

Upstream 100 bases:

>100_bases
AATGGCGCGCGATATGGCTGTGGCGCGGGGCGAGACCCGATGAGCCTGCGTCCTTGGCCGATGGCGTTGCGGGGCTGTCT
GCCCCCCTGGAGCATCGAAG

Downstream 100 bases:

>100_bases
AAAAGGGGCCACGTGCGGGCCCCTGTTTCGTTGCGATTAGCGCTATTCAGCGGCCTCTTGCGGCGGTTTGTCACCACCAC
CGCTGTCACCGCCCTCGCCC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MPHPPERCMAKMKERRMVAIDGLPPLRDVIAAHGLSARKALGQNFLLDLNLTAKIARLAGDLTSVDVLEVGPGPGGLTRG
LLAEGARRVVAVEKDPRCLPVLAEIEAIYPGRLKVLNADALELDWAADLQAPRKIVANLPYNVGTELLVRWLTPASWPPP
WESLTLMFQREVAERIVAQPGSKTYGRLAILSQWRADPRIVMGLPPEAFTPPPKVHSAVVHFTALPAPRFPADARVLTRV
VAAAFGQRRKMLRAALKGLAPDIEDRLVAAGLKPTDRAEQVPLEGFCALARVMEDVITPSHGREA

Sequences:

>Translated_305_residues
MPHPPERCMAKMKERRMVAIDGLPPLRDVIAAHGLSARKALGQNFLLDLNLTAKIARLAGDLTSVDVLEVGPGPGGLTRG
LLAEGARRVVAVEKDPRCLPVLAEIEAIYPGRLKVLNADALELDWAADLQAPRKIVANLPYNVGTELLVRWLTPASWPPP
WESLTLMFQREVAERIVAQPGSKTYGRLAILSQWRADPRIVMGLPPEAFTPPPKVHSAVVHFTALPAPRFPADARVLTRV
VAAAFGQRRKMLRAALKGLAPDIEDRLVAAGLKPTDRAEQVPLEGFCALARVMEDVITPSHGREA
>Mature_304_residues
PHPPERCMAKMKERRMVAIDGLPPLRDVIAAHGLSARKALGQNFLLDLNLTAKIARLAGDLTSVDVLEVGPGPGGLTRGL
LAEGARRVVAVEKDPRCLPVLAEIEAIYPGRLKVLNADALELDWAADLQAPRKIVANLPYNVGTELLVRWLTPASWPPPW
ESLTLMFQREVAERIVAQPGSKTYGRLAILSQWRADPRIVMGLPPEAFTPPPKVHSAVVHFTALPAPRFPADARVLTRVV
AAAFGQRRKMLRAALKGLAPDIEDRLVAAGLKPTDRAEQVPLEGFCALARVMEDVITPSHGREA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=299, Percent_Identity=37.7926421404682, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI7657198, Length=237, Percent_Identity=37.5527426160338, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786236, Length=269, Percent_Identity=33.0855018587361, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=33.8983050847458, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25146882, Length=270, Percent_Identity=30.7407407407407, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=194, Percent_Identity=32.4742268041237, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357273, Length=256, Percent_Identity=35.9375, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21358017, Length=237, Percent_Identity=35.0210970464135, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 33199; Mature: 33068

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPPERCMAKMKERRMVAIDGLPPLRDVIAAHGLSARKALGQNFLLDLNLTAKIARLAG
CCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCHHHHHHCCCEEEECCHHHHHHHHHC
DLTSVDVLEVGPGPGGLTRGLLAEGARRVVAVEKDPRCLPVLAEIEAIYPGRLKVLNADA
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCC
LELDWAADLQAPRKIVANLPYNVGTELLVRWLTPASWPPPWESLTLMFQREVAERIVAQP
EECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GSKTYGRLAILSQWRADPRIVMGLPPEAFTPPPKVHSAVVHFTALPAPRFPADARVLTRV
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHH
VAAAFGQRRKMLRAALKGLAPDIEDRLVAAGLKPTDRAEQVPLEGFCALARVMEDVITPS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCC
HGREA
CCCCC
>Mature Secondary Structure 
PHPPERCMAKMKERRMVAIDGLPPLRDVIAAHGLSARKALGQNFLLDLNLTAKIARLAG
CCCHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCHHHHHHCCCEEEECCHHHHHHHHHC
DLTSVDVLEVGPGPGGLTRGLLAEGARRVVAVEKDPRCLPVLAEIEAIYPGRLKVLNADA
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCC
LELDWAADLQAPRKIVANLPYNVGTELLVRWLTPASWPPPWESLTLMFQREVAERIVAQP
EECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GSKTYGRLAILSQWRADPRIVMGLPPEAFTPPPKVHSAVVHFTALPAPRFPADARVLTRV
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHH
VAAAFGQRRKMLRAALKGLAPDIEDRLVAAGLKPTDRAEQVPLEGFCALARVMEDVITPS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCC
HGREA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA