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The map label for this gene is rihA [H]

Identifier: 89054295

GI number: 89054295

Start: 1793050

End: 1793985

Strand: Reverse

Name: rihA [H]

Synonym: Jann_1804

Alternate gene names: 89054295

Gene position: 1793985-1793050 (Counterclockwise)

Preceding gene: 89054300

Following gene: 89054294

Centisome position: 41.55

GC content: 62.93

Gene sequence:

>936_bases
ATGGCGCGCAAGATTATCATCGACACGGATCCGGGCCAGGATGACGCAGTCGCAATTCTGCTGGCCCTCGCCTCACCGGA
ATTGGAGGTGATGGGGGTGACGGCTGTGGCGGGCAATGTGCCCCTGGAGCTAACCGCCAAAAACGCGCGGATCGTATGCG
AGCTGGCGGGTCGCGCGGACGTGCCGGTGTTCGCAGGCTGCGACAGACCATTGCGCCACGATCTGGTGACGGCAGAGCAT
GTGCACGGCAAGACCGGGTTGGACGGGCCTGAACTGCCAGAGCCGACGATGCCGTTGCAAGACGCGCACGGCGTCGATTT
CATTGTCGACACCTTGCGCCGTGAGCCCGCGGGCACGGTAACCCTCTGCCCCCTCGGCCCACTCACCAATATCGCGACCG
CCTTTGACCGCGCACCGGACATTGTTGAGAAAGTCCAACAGATTGTCCTGATGGGGGGCGCGTATTTCGAGGTGGGCAAC
ATCACACCTGCCGCCGAATTCAACATCTACGTGGACCCGGAGGCGGCGGACATCGTCTTCAAATCCGGCTGTGACATCGT
GGTTATGGGCCTTGACGTGACCCACAAGGCGCTAACCACCGCGCCGCGTGTGCAGGCGTTCCGCGATATGGGCACGCGTC
CCGGCACGATGGTTGCGGAATGGACGGATTTCTTCGAGAGGTTCGATAAGGAGAAGTACGGCAGTGCGGGCGCGCCGCTC
CATGACCCGTGCGTCATCGCCTATCTGATCGCGCCGGAGCTGTTTTCCGGCCGGGTCATCAATGTGGAGATCGAGACCCG
GTCCGACCTGACACGCGGCATGACCGTGGCCGATTGGTGGGGGGTGACGGACCGCGAAAAGAACGCGATGTTCATCGGCG
ACCTGGACGCGGACGGCTTCTTCGCGCTGTTGGTTGACCGGATCGGGCGGCTATGA

Upstream 100 bases:

>100_bases
TGAGCCCGTATTGCCAACCCGGCCGCGCTAAATGTGTCAATGGTATACTTGCATATCATGCCGCCCTCCGCTCCTATCAG
GACGCAGATCGGAGGGGCCC

Downstream 100 bases:

>100_bases
GGGCAGCGCTAGCCTGCGCCCTCTGCCTCGCCCCCCTGCCCGCGACAGCCGACGGCCCGGCGCTGGCCCATCTGACGGTC
GCGGGGACGACCATCGCGCT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MARKIIIDTDPGQDDAVAILLALASPELEVMGVTAVAGNVPLELTAKNARIVCELAGRADVPVFAGCDRPLRHDLVTAEH
VHGKTGLDGPELPEPTMPLQDAHGVDFIVDTLRREPAGTVTLCPLGPLTNIATAFDRAPDIVEKVQQIVLMGGAYFEVGN
ITPAAEFNIYVDPEAADIVFKSGCDIVVMGLDVTHKALTTAPRVQAFRDMGTRPGTMVAEWTDFFERFDKEKYGSAGAPL
HDPCVIAYLIAPELFSGRVINVEIETRSDLTRGMTVADWWGVTDREKNAMFIGDLDADGFFALLVDRIGRL

Sequences:

>Translated_311_residues
MARKIIIDTDPGQDDAVAILLALASPELEVMGVTAVAGNVPLELTAKNARIVCELAGRADVPVFAGCDRPLRHDLVTAEH
VHGKTGLDGPELPEPTMPLQDAHGVDFIVDTLRREPAGTVTLCPLGPLTNIATAFDRAPDIVEKVQQIVLMGGAYFEVGN
ITPAAEFNIYVDPEAADIVFKSGCDIVVMGLDVTHKALTTAPRVQAFRDMGTRPGTMVAEWTDFFERFDKEKYGSAGAPL
HDPCVIAYLIAPELFSGRVINVEIETRSDLTRGMTVADWWGVTDREKNAMFIGDLDADGFFALLVDRIGRL
>Mature_310_residues
ARKIIIDTDPGQDDAVAILLALASPELEVMGVTAVAGNVPLELTAKNARIVCELAGRADVPVFAGCDRPLRHDLVTAEHV
HGKTGLDGPELPEPTMPLQDAHGVDFIVDTLRREPAGTVTLCPLGPLTNIATAFDRAPDIVEKVQQIVLMGGAYFEVGNI
TPAAEFNIYVDPEAADIVFKSGCDIVVMGLDVTHKALTTAPRVQAFRDMGTRPGTMVAEWTDFFERFDKEKYGSAGAPLH
DPCVIAYLIAPELFSGRVINVEIETRSDLTRGMTVADWWGVTDREKNAMFIGDLDADGFFALLVDRIGRL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=49.5145631067961, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788486, Length=302, Percent_Identity=41.3907284768212, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1786213, Length=302, Percent_Identity=38.7417218543046, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=37.5661375661376, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17565698, Length=291, Percent_Identity=27.4914089347079, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=33.5907335907336, Blast_Score=134, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24583915, Length=190, Percent_Identity=33.1578947368421, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641837, Length=341, Percent_Identity=27.8592375366569, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24641839, Length=192, Percent_Identity=31.7708333333333, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33642; Mature: 33511

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKIIIDTDPGQDDAVAILLALASPELEVMGVTAVAGNVPLELTAKNARIVCELAGRAD
CCCEEEEECCCCCCHHEEEEEECCCCCEEEEEEEEEECCCCEEEEECCCEEEEEECCCCC
VPVFAGCDRPLRHDLVTAEHVHGKTGLDGPELPEPTMPLQDAHGVDFIVDTLRREPAGTV
CCEEECCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
TLCPLGPLTNIATAFDRAPDIVEKVQQIVLMGGAYFEVGNITPAAEFNIYVDPEAADIVF
EEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCHHEEEE
KSGCDIVVMGLDVTHKALTTAPRVQAFRDMGTRPGTMVAEWTDFFERFDKEKYGSAGAPL
ECCCCEEEEECCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCC
HDPCVIAYLIAPELFSGRVINVEIETRSDLTRGMTVADWWGVTDREKNAMFIGDLDADGF
CCCCEEEEEECHHHHCCEEEEEEEECCCHHHHCCEEEHHCCCCCCCCCEEEEEECCCCCH
FALLVDRIGRL
HHHHHHHHCCC
>Mature Secondary Structure 
ARKIIIDTDPGQDDAVAILLALASPELEVMGVTAVAGNVPLELTAKNARIVCELAGRAD
CCEEEEECCCCCCHHEEEEEECCCCCEEEEEEEEEECCCCEEEEECCCEEEEEECCCCC
VPVFAGCDRPLRHDLVTAEHVHGKTGLDGPELPEPTMPLQDAHGVDFIVDTLRREPAGTV
CCEEECCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
TLCPLGPLTNIATAFDRAPDIVEKVQQIVLMGGAYFEVGNITPAAEFNIYVDPEAADIVF
EEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCHHEEEE
KSGCDIVVMGLDVTHKALTTAPRVQAFRDMGTRPGTMVAEWTDFFERFDKEKYGSAGAPL
ECCCCEEEEECCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCC
HDPCVIAYLIAPELFSGRVINVEIETRSDLTRGMTVADWWGVTDREKNAMFIGDLDADGF
CCCCEEEEEECHHHHCCEEEEEEEECCCHHHHCCEEEHHCCCCCCCCCEEEEEECCCCCH
FALLVDRIGRL
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA