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The map label for this gene is eno

Identifier: 89054288

GI number: 89054288

Start: 1785942

End: 1787219

Strand: Reverse

Name: eno

Synonym: Jann_1797

Alternate gene names: 89054288

Gene position: 1787219-1785942 (Counterclockwise)

Preceding gene: 89054289

Following gene: 89054287

Centisome position: 41.39

GC content: 61.66

Gene sequence:

>1278_bases
ATGAGCGTTATCATCGACATCCATGCCCGCGAGATCCTCGATAGCAGGGGCAACCCCACGGTGGAGGTTGATGTGACCCT
GGAAGATGGCACCATGGGCCGGGCTGCCGTGCCCTCGGGCGCCTCAACCGGTGCCTATGAGGCGGTCGAAAAACGCGACG
GCGATAAGGCGCGCTACAAGGGCAAAGGCGTGCTGGCCGCCGTGGAAGCGGTGAACGGTGAGATTGCCGATACACTGGTG
GGCTTTGACGCCACCGAACAAGTCGCCATCGACCAGACGATGTGTGAGTTGGACGGCACCGATAACAAGGGCCGTCTGGG
CGCGAATGCCATCCTCGGCGTATCGCTGGCCACGGCCAAGGCAGCGGCGGATTTCACGGCGCAGCCTTTGTATCGCTACG
TGGGCGGCACGATGGCCCACGTCTTGCCCGTGCCGATGATGAACATCATCAACGGTGGCGAACATGCCGACAACCCCATC
GACATCCAGGAATTCATGATCATGCCCGTGTCGGCCACGTCGATTGCGGAGGCGGTGCGCATGGGGTCCGAAGTGTTCCA
CACGCTGAAGAGCGAATTGTCCGCGGCGGGTTTGTCCACCGGCATCGGGGATGAGGGCGGCTTTGCGCCCAACCTGTCAT
CCACCCGTGACGCGTTGGATTTCGTGCTGAAAAGCATTGAGAAGGCAGGATATGCGCCAGGTGACGACATGGTTCTGGCC
CTCGATTGCGCGGCGACGGAATACTACCGCGACGGCAAGTACGAGCTGTCGGGCGAAGGCAAGAGCCTGACATCGGACGA
AAACGTCGCCTATCTGGCCGCGCTGGTGGCGAATTATCCGATTTTCTCGATCGAGGACGGCATGGGAGAGGACGATTGGG
ACGGCTGGATCGCCCTGACCGAGGCCCTGGGGGATAAGGTGCAACTTGTGGGCGATGATCTCTTCGTCACCAACCCGGCC
CGCTTGAAGGACGGGATCGACCGCAAGGCCGCGAACTCGCTTCTGGTGAAGGTCAACCAGATCGGCACCCTCACCGAAAC
GCTGGCCGCCGTGAACATGGCGACACGGGCGGGATTCACCTCCGTCATGTCCCACCGCTCGGGCGAGACGGAGGATGCAA
CCATTGCCGATCTCGCCGTGGCGACCAATTGCGGGCAGATCAAGACCGGCTCACTGGCGCGATCCGACCGGTTGGCGAAG
TACAACCAACTGATCCGGATCGAAGAGCAGCTGGAAGAAACGGCAAACTTCGCCGGACGGTCCATTTTGCGCGGATAG

Upstream 100 bases:

>100_bases
GCGACGACCTGAGCCCGCCTGTTACCGCTAACGGAAATGTTTGCCCCGGATGAAACCTCTGCTATAGCGCCGGCAACCCC
ATACCTTAGGAGACCAATCC

Downstream 100 bases:

>100_bases
AAAGCCGACACCCCTAGAATACAGCCGATGCGGGGAATGCGCGGCCAGGGTGTCAGGGCTTTGAAAGCTGTTAAGTCGGT
CAAAAACCTGACGAAAAGCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSVIIDIHAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAYEAVEKRDGDKARYKGKGVLAAVEAVNGEIADTLV
GFDATEQVAIDQTMCELDGTDNKGRLGANAILGVSLATAKAAADFTAQPLYRYVGGTMAHVLPVPMMNIINGGEHADNPI
DIQEFMIMPVSATSIAEAVRMGSEVFHTLKSELSAAGLSTGIGDEGGFAPNLSSTRDALDFVLKSIEKAGYAPGDDMVLA
LDCAATEYYRDGKYELSGEGKSLTSDENVAYLAALVANYPIFSIEDGMGEDDWDGWIALTEALGDKVQLVGDDLFVTNPA
RLKDGIDRKAANSLLVKVNQIGTLTETLAAVNMATRAGFTSVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEQLEETANFAGRSILRG

Sequences:

>Translated_425_residues
MSVIIDIHAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAYEAVEKRDGDKARYKGKGVLAAVEAVNGEIADTLV
GFDATEQVAIDQTMCELDGTDNKGRLGANAILGVSLATAKAAADFTAQPLYRYVGGTMAHVLPVPMMNIINGGEHADNPI
DIQEFMIMPVSATSIAEAVRMGSEVFHTLKSELSAAGLSTGIGDEGGFAPNLSSTRDALDFVLKSIEKAGYAPGDDMVLA
LDCAATEYYRDGKYELSGEGKSLTSDENVAYLAALVANYPIFSIEDGMGEDDWDGWIALTEALGDKVQLVGDDLFVTNPA
RLKDGIDRKAANSLLVKVNQIGTLTETLAAVNMATRAGFTSVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEQLEETANFAGRSILRG
>Mature_424_residues
SVIIDIHAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAYEAVEKRDGDKARYKGKGVLAAVEAVNGEIADTLVG
FDATEQVAIDQTMCELDGTDNKGRLGANAILGVSLATAKAAADFTAQPLYRYVGGTMAHVLPVPMMNIINGGEHADNPID
IQEFMIMPVSATSIAEAVRMGSEVFHTLKSELSAAGLSTGIGDEGGFAPNLSSTRDALDFVLKSIEKAGYAPGDDMVLAL
DCAATEYYRDGKYELSGEGKSLTSDENVAYLAALVANYPIFSIEDGMGEDDWDGWIALTEALGDKVQLVGDDLFVTNPAR
LKDGIDRKAANSLLVKVNQIGTLTETLAAVNMATRAGFTSVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEQLEETANFAGRSILRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=52.2041763341067, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.8120649651972, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=50.9345794392523, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=50.9345794392523, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=46.9483568075117, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=61.6279069767442, Blast_Score=521, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.2407407407407, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.2407407407407, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=47.3684210526316, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=49.7695852534562, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=437, Percent_Identity=48.9702517162471, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=437, Percent_Identity=48.512585812357, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=437, Percent_Identity=48.512585812357, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=49.3087557603687, Blast_Score=371, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=50.3480278422274, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=50.3480278422274, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=50.3480278422274, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=50.3480278422274, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=50.3480278422274, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=50.3480278422274, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_JANSC (Q28RE8)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509739.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q28RE8
- SMR:   Q28RE8
- STRING:   Q28RE8
- GeneID:   3934246
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1797
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q28RE8
- ProtClustDB:   PRK00077
- BioCyc:   JSP290400:JANN_1797-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44928; Mature: 44797

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIDIHAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAYEAVEKRDGDKARYK
CEEEEEEHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHCC
GKGVLAAVEAVNGEIADTLVGFDATEQVAIDQTMCELDGTDNKGRLGANAILGVSLATAK
CCCCEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHH
AAADFTAQPLYRYVGGTMAHVLPVPMMNIINGGEHADNPIDIQEFMIMPVSATSIAEAVR
HHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MGSEVFHTLKSELSAAGLSTGIGDEGGFAPNLSSTRDALDFVLKSIEKAGYAPGDDMVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEYYRDGKYELSGEGKSLTSDENVAYLAALVANYPIFSIEDGMGEDDWDGWIALT
EECHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
EALGDKVQLVGDDLFVTNPARLKDGIDRKAANSLLVKVNQIGTLTETLAAVNMATRAGFT
HHCCCEEEEECCCEEEECCHHHHHCCCHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHH
SVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEQLEETANFAGR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILRG
HHCCC
>Mature Secondary Structure 
SVIIDIHAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAYEAVEKRDGDKARYK
EEEEEEHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHCC
GKGVLAAVEAVNGEIADTLVGFDATEQVAIDQTMCELDGTDNKGRLGANAILGVSLATAK
CCCCEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHH
AAADFTAQPLYRYVGGTMAHVLPVPMMNIINGGEHADNPIDIQEFMIMPVSATSIAEAVR
HHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MGSEVFHTLKSELSAAGLSTGIGDEGGFAPNLSSTRDALDFVLKSIEKAGYAPGDDMVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEYYRDGKYELSGEGKSLTSDENVAYLAALVANYPIFSIEDGMGEDDWDGWIALT
EECHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
EALGDKVQLVGDDLFVTNPARLKDGIDRKAANSLLVKVNQIGTLTETLAAVNMATRAGFT
HHCCCEEEEECCCEEEECCHHHHHCCCHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHH
SVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEQLEETANFAGR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILRG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA