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The map label for this gene is pyrC

Identifier: 89054233

GI number: 89054233

Start: 1734724

End: 1735767

Strand: Reverse

Name: pyrC

Synonym: Jann_1742

Alternate gene names: 89054233

Gene position: 1735767-1734724 (Counterclockwise)

Preceding gene: 89054248

Following gene: 89054232

Centisome position: 40.2

GC content: 64.18

Gene sequence:

>1044_bases
ATGACCCAATCCCTCACGATCCGCCGCCCCGACGACTGGCACCTGCACCTGCGCGACGGCGCGATGATGCGGGCCGTTCT
GCCTGAAACCGCCCGTCATTTTGCCCGCGCGATCATCATGCCCAACCTCGTGCCGCCCGTGGTCACAGCCCAGGATGCGC
TGGCCTACCGCGACCGGATTGAGGCGGCGCTGCCGGACGGCGCGGATTTCACGCCACTGATGACGCTTTATCTAACCGAA
CAGACGGACCCGGACGACGTGGAGGCGGCGTTTGCCGATGGGCTGATCCATGCGGTCAAACTCTATCCGGCGGGGGCCAC
GACAAATTCCCAATCCGGCGTTGCGACGCTGAAAAACGTCTATCCGGTGCTGGAGCGTATGGCCCAGATCGGCCTGCCGC
TGTGTATCCATGGCGAGGTCACCGATCCGCAGGTCGACATCTTCGACCGGGAACAGGCCTTCATCGATACCGTCCTGATC
CCGCTGCGCGCCGACCTGCCGGACCTGAAGGTCACGCTGGAGCATATCACCACCTCCCACGGCATCGATTATGTGGTGGG
CGCGGACGGGGACATTGCCGGCACGATCACAACGCATCACCTGATGATCAACCGCAACCATATGCTCGTGGGCGGCATCC
GACCGCACTATTATTGCCTGCCCATCGTCAAACGCGCCTCCCACCAACACGCCCTGCGCCGGGCCGCGACATCGGGCCAC
CCGCGCTTCTTTCTGGGCACCGACTCGGCCCCCCACCTGGACGGCGCGAAGGAAACCGCCTGCGGGTGCGCGGGCGTCTT
CTCAGCCACCAACACGATGTCCTGCCTCGCCCATGTGTTCGACGAAGACGGCGCGCTGGTGAACCTTGAGGCCTTCACCT
CCCTCAACGGCCCCGCCCGCTATGGCCTGGCGCCGAACGCGGCCACGATGACGCTGGAAAAGCGCGACCATCCCACCAAT
TACCCTGCCAAATTTGACACAGATGACGGCCCCCTGACCCTCTTCGATCCCTCCACGCCGCTCTATTGGCATGTGAAGGA
CTGA

Upstream 100 bases:

>100_bases
GATCAGCGCAGGGCCGACGCGGATCACCTTTGCCCGTGATCCCGCCTCAAATCGCGCGCCCCTCTTTCCGCACGTCGCGA
CCTGCTATAACACACGGCCC

Downstream 100 bases:

>100_bases
CCCCTCCATCCCCGTTCTTCAAATATCCCCCCCGGAGGGTCCGCCCCCTCCCCCTCGCACCACCGCCAAAGGTTTCGCCA
TGATCCCCTCCACCTACCCC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTQSLTIRRPDDWHLHLRDGAMMRAVLPETARHFARAIIMPNLVPPVVTAQDALAYRDRIEAALPDGADFTPLMTLYLTE
QTDPDDVEAAFADGLIHAVKLYPAGATTNSQSGVATLKNVYPVLERMAQIGLPLCIHGEVTDPQVDIFDREQAFIDTVLI
PLRADLPDLKVTLEHITTSHGIDYVVGADGDIAGTITTHHLMINRNHMLVGGIRPHYYCLPIVKRASHQHALRRAATSGH
PRFFLGTDSAPHLDGAKETACGCAGVFSATNTMSCLAHVFDEDGALVNLEAFTSLNGPARYGLAPNAATMTLEKRDHPTN
YPAKFDTDDGPLTLFDPSTPLYWHVKD

Sequences:

>Translated_347_residues
MTQSLTIRRPDDWHLHLRDGAMMRAVLPETARHFARAIIMPNLVPPVVTAQDALAYRDRIEAALPDGADFTPLMTLYLTE
QTDPDDVEAAFADGLIHAVKLYPAGATTNSQSGVATLKNVYPVLERMAQIGLPLCIHGEVTDPQVDIFDREQAFIDTVLI
PLRADLPDLKVTLEHITTSHGIDYVVGADGDIAGTITTHHLMINRNHMLVGGIRPHYYCLPIVKRASHQHALRRAATSGH
PRFFLGTDSAPHLDGAKETACGCAGVFSATNTMSCLAHVFDEDGALVNLEAFTSLNGPARYGLAPNAATMTLEKRDHPTN
YPAKFDTDDGPLTLFDPSTPLYWHVKD
>Mature_346_residues
TQSLTIRRPDDWHLHLRDGAMMRAVLPETARHFARAIIMPNLVPPVVTAQDALAYRDRIEAALPDGADFTPLMTLYLTEQ
TDPDDVEAAFADGLIHAVKLYPAGATTNSQSGVATLKNVYPVLERMAQIGLPLCIHGEVTDPQVDIFDREQAFIDTVLIP
LRADLPDLKVTLEHITTSHGIDYVVGADGDIAGTITTHHLMINRNHMLVGGIRPHYYCLPIVKRASHQHALRRAATSGHP
RFFLGTDSAPHLDGAKETACGCAGVFSATNTMSCLAHVFDEDGALVNLEAFTSLNGPARYGLAPNAATMTLEKRDHPTNY
PAKFDTDDGPLTLFDPSTPLYWHVKD

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=346, Percent_Identity=53.4682080924855, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323452, Length=320, Percent_Identity=34.6875, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_JANSC (Q28RK3)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509684.1
- ProteinModelPortal:   Q28RK3
- SMR:   Q28RK3
- STRING:   Q28RK3
- GeneID:   3934190
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1742
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q28RK3
- ProtClustDB:   PRK05451
- BioCyc:   JSP290400:JANN_1742-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 37944; Mature: 37812

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLTIRRPDDWHLHLRDGAMMRAVLPETARHFARAIIMPNLVPPVVTAQDALAYRDRI
CCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EAALPDGADFTPLMTLYLTEQTDPDDVEAAFADGLIHAVKLYPAGATTNSQSGVATLKNV
HHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHH
YPVLERMAQIGLPLCIHGEVTDPQVDIFDREQAFIDTVLIPLRADLPDLKVTLEHITTSH
HHHHHHHHHCCCCEEEECCCCCCCEEEECCHHHHHHHEEEECCCCCCHHEEEHHHHHHCC
GIDYVVGADGDIAGTITTHHLMINRNHMLVGGIRPHYYCLPIVKRASHQHALRRAATSGH
CCCEEEECCCCEEEEEEEEEEEEECCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCC
PRFFLGTDSAPHLDGAKETACGCAGVFSATNTMSCLAHVFDEDGALVNLEAFTSLNGPAR
CEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCEE
YGLAPNAATMTLEKRDHPTNYPAKFDTDDGPLTLFDPSTPLYWHVKD
CCCCCCCEEEEEECCCCCCCCCCEECCCCCCEEEECCCCCEEEEECC
>Mature Secondary Structure 
TQSLTIRRPDDWHLHLRDGAMMRAVLPETARHFARAIIMPNLVPPVVTAQDALAYRDRI
CCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EAALPDGADFTPLMTLYLTEQTDPDDVEAAFADGLIHAVKLYPAGATTNSQSGVATLKNV
HHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHH
YPVLERMAQIGLPLCIHGEVTDPQVDIFDREQAFIDTVLIPLRADLPDLKVTLEHITTSH
HHHHHHHHHCCCCEEEECCCCCCCEEEECCHHHHHHHEEEECCCCCCHHEEEHHHHHHCC
GIDYVVGADGDIAGTITTHHLMINRNHMLVGGIRPHYYCLPIVKRASHQHALRRAATSGH
CCCEEEECCCCEEEEEEEEEEEEECCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCC
PRFFLGTDSAPHLDGAKETACGCAGVFSATNTMSCLAHVFDEDGALVNLEAFTSLNGPAR
CEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCEE
YGLAPNAATMTLEKRDHPTNYPAKFDTDDGPLTLFDPSTPLYWHVKD
CCCCCCCEEEEEECCCCCCCCCCEECCCCCCEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA