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The map label for this gene is pyrE

Identifier: 89054232

GI number: 89054232

Start: 1733958

End: 1734644

Strand: Reverse

Name: pyrE

Synonym: Jann_1741

Alternate gene names: 89054232

Gene position: 1734644-1733958 (Counterclockwise)

Preceding gene: 89054233

Following gene: 89054231

Centisome position: 40.17

GC content: 62.45

Gene sequence:

>687_bases
ATGATCCCCTCCACCTACCCCAATAACTCCACCATGGCCGCGATGACCGCCCGTATGTTGCTCGACATCCGCGCGGTTCA
TTTCAACACCGATGAGCCCTTCACCCACGCCTCGGGCCTGCAAGCCCCCACCTATATCGACTGCCGCAAGCTGATCTCTC
ACCCGCGTATCCGGTCCACGGCGATGGATTTCCTGACCTGCAAAGTCATGCGTGATGCAGGTCTTGAGGCGTTTGACAAT
ATCGCGGGCGGGGAGACGGCGGGCATCCCCTTTGCGGCGTTTGTGGCCGAGCGGATGGCGCTGCCCATGTCCTATGTGCG
CAAGAAGCCAAAAGGCTACGGCAAAACCGCCCAGATTGAGGGCGAAATGCCGGAAGGGGCCCGCGTGCTGCTGGTGGAGG
ACCTCACCACCGATGGCGGCTCCAAACTGAAATTCGTCGATGCCATTCGCAACGCTGGTGCCACCTGTGCGCACACGGCC
GTGGTCTTTTCCTACGGCAATCTGCCCGAGACCGAGGCCAATCTGGCGGCCCACGGTCTGACGCTGCATTCCCTGACCAC
CTGGGCCGATGTCATCGCGGAGGCGCGCCGCGGGAGCGACTTCAATGAAGCCACCCTGACAGAGGTCGAGCGATTCTTGT
CCGACCCGGGCGGCTGGCGCGGCGACCGGGGCCTTAACACAGATTAA

Upstream 100 bases:

>100_bases
TATTGGCATGTGAAGGACTGACCCCTCCATCCCCGTTCTTCAAATATCCCCCCCGGAGGGTCCGCCCCCTCCCCCTCGCA
CCACCGCCAAAGGTTTCGCC

Downstream 100 bases:

>100_bases
CCGGTTCTGACGTCACGTTCATCATCCCCAATATTAACTGTCGGCGTGGCGATTTCGCCACGTAACGATTGCATGACAGG
TAGATCTTGACGCAGGCCCA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MIPSTYPNNSTMAAMTARMLLDIRAVHFNTDEPFTHASGLQAPTYIDCRKLISHPRIRSTAMDFLTCKVMRDAGLEAFDN
IAGGETAGIPFAAFVAERMALPMSYVRKKPKGYGKTAQIEGEMPEGARVLLVEDLTTDGGSKLKFVDAIRNAGATCAHTA
VVFSYGNLPETEANLAAHGLTLHSLTTWADVIAEARRGSDFNEATLTEVERFLSDPGGWRGDRGLNTD

Sequences:

>Translated_228_residues
MIPSTYPNNSTMAAMTARMLLDIRAVHFNTDEPFTHASGLQAPTYIDCRKLISHPRIRSTAMDFLTCKVMRDAGLEAFDN
IAGGETAGIPFAAFVAERMALPMSYVRKKPKGYGKTAQIEGEMPEGARVLLVEDLTTDGGSKLKFVDAIRNAGATCAHTA
VVFSYGNLPETEANLAAHGLTLHSLTTWADVIAEARRGSDFNEATLTEVERFLSDPGGWRGDRGLNTD
>Mature_228_residues
MIPSTYPNNSTMAAMTARMLLDIRAVHFNTDEPFTHASGLQAPTYIDCRKLISHPRIRSTAMDFLTCKVMRDAGLEAFDN
IAGGETAGIPFAAFVAERMALPMSYVRKKPKGYGKTAQIEGEMPEGARVLLVEDLTTDGGSKLKFVDAIRNAGATCAHTA
VVFSYGNLPETEANLAAHGLTLHSLTTWADVIAEARRGSDFNEATLTEVERFLSDPGGWRGDRGLNTD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=196, Percent_Identity=29.5918367346939, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17508631, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17933654, Length=225, Percent_Identity=28.4444444444444, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_JANSC (Q28RK4)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509683.1
- ProteinModelPortal:   Q28RK4
- SMR:   Q28RK4
- STRING:   Q28RK4
- GeneID:   3934189
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1741
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- ProtClustDB:   PRK00455
- BioCyc:   JSP290400:JANN_1741-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 24728; Mature: 24728

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 107-107 BINDING 108-108 BINDING 111-111 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSTYPNNSTMAAMTARMLLDIRAVHFNTDEPFTHASGLQAPTYIDCRKLISHPRIRST
CCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
AMDFLTCKVMRDAGLEAFDNIAGGETAGIPFAAFVAERMALPMSYVRKKPKGYGKTAQIE
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEC
GEMPEGARVLLVEDLTTDGGSKLKFVDAIRNAGATCAHTAVVFSYGNLPETEANLAAHGL
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCCCHHHHHHCCC
TLHSLTTWADVIAEARRGSDFNEATLTEVERFLSDPGGWRGDRGLNTD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIPSTYPNNSTMAAMTARMLLDIRAVHFNTDEPFTHASGLQAPTYIDCRKLISHPRIRST
CCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
AMDFLTCKVMRDAGLEAFDNIAGGETAGIPFAAFVAERMALPMSYVRKKPKGYGKTAQIE
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEC
GEMPEGARVLLVEDLTTDGGSKLKFVDAIRNAGATCAHTAVVFSYGNLPETEANLAAHGL
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCCCHHHHHHCCC
TLHSLTTWADVIAEARRGSDFNEATLTEVERFLSDPGGWRGDRGLNTD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA