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The map label for this gene is dnaB [H]

Identifier: 89054231

GI number: 89054231

Start: 1732179

End: 1733684

Strand: Reverse

Name: dnaB [H]

Synonym: Jann_1740

Alternate gene names: 89054231

Gene position: 1733684-1732179 (Counterclockwise)

Preceding gene: 89054232

Following gene: 89054230

Centisome position: 40.15

GC content: 62.28

Gene sequence:

>1506_bases
ATGAACGATCTGGCCGCCTTTAACCCATCACTCCCTGCGGACCCCGAAACCGTTGAGCAAGCCCCCGTCCTGCCCCACAA
CATCGAGGCTGAACAGCAGCTTTTGGGCGCGATCTTGACATCCAACGACGTCCTGGACCGCGTCGATGACCTCGTCAAAC
CCGACCATTTTCATGACCCCGTCCATGCGGAGATCTTCACCATGGCCGCCGCGCGCATCGCCAAGGGCCTGCAAACCGAC
GCGACGACGCTGAAGACCTTCGCCTCCGACATTCCCGGGCTGAAAGATCTGGGCGGGGCGGAATATCTGGTGAAATTGCA
ACTGTCGGCCATCGCGACCTCTGCCGCACGGGACTATGCGCAACTGATCCATGACCTCGCCGTCCGGCGTGAGCTGATTG
AGTTGGGCAACCGCGTCACCGACCGCGCCCGCGCCATGCGCGACGACGTGGCCCCCGACGACCAAATCGTGGAAGCGGAA
AGCGAGTTGTTCGCGCTGGCCTCCACCGGATCGACCAACAAGGGCTTTCAATCCTTCCTCCATGCCCTGCACGACGCGGT
GCACATGGCGAACGAGGCCTATAACCGCCAAGGCGGGTTGGCGGGCGTCTCTACCGGCCTCACCGATCTCGACCGAATGC
TCGGCGGCTTGCACAAATCAGACCTTTTGATCCTAGCCGGCCGTCCCTCGATGGGGAAGACGTCCCTGGCCACAAACGTG
GCCTTCAACGTCGCCAAGGCCTACCGCGAAGGCGTCAAAGAGGATGGGTCCGAGGGCACCGTCGATGGCGGAGTGGTAGG
CTTCTTCTCTCTGGAGATGTCATCGGCACAGCTTGCTCAACGGATCCTGTCGGAGGCCGCCGAGATCCCGTCCGAGTTGA
TCCGCAAGGGCGACCTGAACGAGCAGGAATTCCAACGCTATCTGAAGGCAGCGGGCGATCTGGAACGTTGCCCGCTTTAC
ATCGATGACACGCCCGCGCTGCCCATCGCCCAGGTGGCCGCGCGGGCGCGACGCCTCAAGCGGTCGCCAAAGGGGCTCGA
TCTGCTAATCATCGACTATCTCCAGCTGCTGAAAGGATCGGGCAAATCCGACAACCGCGTCAATGAGGTGTCTGAGATCA
CCCAAGGCCTCAAAGCCATCGCCAAGGAGTTGGACATTCCCGTCATCGCGCTGTCGCAGCTGTCGCGCCAGGTGGAAAGC
CGCGAGGACAAGCGCCCGCAGCTCTCGGACCTGCGGGAGTCGGGCTCCATCGAGCAGGACGCGGACGTCGTGATGTTCGT
TTACCGTGGCGAATATTACAAAGAACGCGAGAAGCCAGGTGAGGAGAATCTGGAGGCCATGACCAAGTGGCAGCAGGATA
TGGAACATCTGCACGGCAAGGCGGAGGTCATCATCGGCAAGCAACGCCACGGCCCCGTGGGAGCCGTTGAGCTGGCCTTT
GAGGGGCGGTTCACCCGCTTCGGCAATCTCGCCCAACCCCGCCACGGCGCGCCCGAGGGATACTGA

Upstream 100 bases:

>100_bases
GACGGGCCCGCAGGCGCACCCGCTATACCCATCCTCACCGCGCCCGGGCCTGCCAGCCAAACGACCGCGCGGGTGTAACG
AGCGTATCCGGGGGGAGCAC

Downstream 100 bases:

>100_bases
AGCGCGGAAAACTCTTGCAAGAGTTTTCCCCACCGCTTTACAAAGCGGTGTCTCACGTCCTTGCAAGGACGTGCCAAGGC
TCTTGCAAGAGCCTTGACGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNDLAAFNPSLPADPETVEQAPVLPHNIEAEQQLLGAILTSNDVLDRVDDLVKPDHFHDPVHAEIFTMAAARIAKGLQTD
ATTLKTFASDIPGLKDLGGAEYLVKLQLSAIATSAARDYAQLIHDLAVRRELIELGNRVTDRARAMRDDVAPDDQIVEAE
SELFALASTGSTNKGFQSFLHALHDAVHMANEAYNRQGGLAGVSTGLTDLDRMLGGLHKSDLLILAGRPSMGKTSLATNV
AFNVAKAYREGVKEDGSEGTVDGGVVGFFSLEMSSAQLAQRILSEAAEIPSELIRKGDLNEQEFQRYLKAAGDLERCPLY
IDDTPALPIAQVAARARRLKRSPKGLDLLIIDYLQLLKGSGKSDNRVNEVSEITQGLKAIAKELDIPVIALSQLSRQVES
REDKRPQLSDLRESGSIEQDADVVMFVYRGEYYKEREKPGEENLEAMTKWQQDMEHLHGKAEVIIGKQRHGPVGAVELAF
EGRFTRFGNLAQPRHGAPEGY

Sequences:

>Translated_501_residues
MNDLAAFNPSLPADPETVEQAPVLPHNIEAEQQLLGAILTSNDVLDRVDDLVKPDHFHDPVHAEIFTMAAARIAKGLQTD
ATTLKTFASDIPGLKDLGGAEYLVKLQLSAIATSAARDYAQLIHDLAVRRELIELGNRVTDRARAMRDDVAPDDQIVEAE
SELFALASTGSTNKGFQSFLHALHDAVHMANEAYNRQGGLAGVSTGLTDLDRMLGGLHKSDLLILAGRPSMGKTSLATNV
AFNVAKAYREGVKEDGSEGTVDGGVVGFFSLEMSSAQLAQRILSEAAEIPSELIRKGDLNEQEFQRYLKAAGDLERCPLY
IDDTPALPIAQVAARARRLKRSPKGLDLLIIDYLQLLKGSGKSDNRVNEVSEITQGLKAIAKELDIPVIALSQLSRQVES
REDKRPQLSDLRESGSIEQDADVVMFVYRGEYYKEREKPGEENLEAMTKWQQDMEHLHGKAEVIIGKQRHGPVGAVELAF
EGRFTRFGNLAQPRHGAPEGY
>Mature_501_residues
MNDLAAFNPSLPADPETVEQAPVLPHNIEAEQQLLGAILTSNDVLDRVDDLVKPDHFHDPVHAEIFTMAAARIAKGLQTD
ATTLKTFASDIPGLKDLGGAEYLVKLQLSAIATSAARDYAQLIHDLAVRRELIELGNRVTDRARAMRDDVAPDDQIVEAE
SELFALASTGSTNKGFQSFLHALHDAVHMANEAYNRQGGLAGVSTGLTDLDRMLGGLHKSDLLILAGRPSMGKTSLATNV
AFNVAKAYREGVKEDGSEGTVDGGVVGFFSLEMSSAQLAQRILSEAAEIPSELIRKGDLNEQEFQRYLKAAGDLERCPLY
IDDTPALPIAQVAARARRLKRSPKGLDLLIIDYLQLLKGSGKSDNRVNEVSEITQGLKAIAKELDIPVIALSQLSRQVES
REDKRPQLSDLRESGSIEQDADVVMFVYRGEYYKEREKPGEENLEAMTKWQQDMEHLHGKAEVIIGKQRHGPVGAVELAF
EGRFTRFGNLAQPRHGAPEGY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=485, Percent_Identity=38.1443298969072, Blast_Score=294, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54806; Mature: 54806

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLAAFNPSLPADPETVEQAPVLPHNIEAEQQLLGAILTSNDVLDRVDDLVKPDHFHDP
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
VHAEIFTMAAARIAKGLQTDATTLKTFASDIPGLKDLGGAEYLVKLQLSAIATSAARDYA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
QLIHDLAVRRELIELGNRVTDRARAMRDDVAPDDQIVEAESELFALASTGSTNKGFQSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHH
HALHDAVHMANEAYNRQGGLAGVSTGLTDLDRMLGGLHKSDLLILAGRPSMGKTSLATNV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
AFNVAKAYREGVKEDGSEGTVDGGVVGFFSLEMSSAQLAQRILSEAAEIPSELIRKGDLN
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EQEFQRYLKAAGDLERCPLYIDDTPALPIAQVAARARRLKRSPKGLDLLIIDYLQLLKGS
HHHHHHHHHHCCCHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
GKSDNRVNEVSEITQGLKAIAKELDIPVIALSQLSRQVESREDKRPQLSDLRESGSIEQD
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
ADVVMFVYRGEYYKEREKPGEENLEAMTKWQQDMEHLHGKAEVIIGKQRHGPVGAVELAF
CCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEE
EGRFTRFGNLAQPRHGAPEGY
CCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDLAAFNPSLPADPETVEQAPVLPHNIEAEQQLLGAILTSNDVLDRVDDLVKPDHFHDP
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
VHAEIFTMAAARIAKGLQTDATTLKTFASDIPGLKDLGGAEYLVKLQLSAIATSAARDYA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
QLIHDLAVRRELIELGNRVTDRARAMRDDVAPDDQIVEAESELFALASTGSTNKGFQSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHH
HALHDAVHMANEAYNRQGGLAGVSTGLTDLDRMLGGLHKSDLLILAGRPSMGKTSLATNV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHH
AFNVAKAYREGVKEDGSEGTVDGGVVGFFSLEMSSAQLAQRILSEAAEIPSELIRKGDLN
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EQEFQRYLKAAGDLERCPLYIDDTPALPIAQVAARARRLKRSPKGLDLLIIDYLQLLKGS
HHHHHHHHHHCCCHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
GKSDNRVNEVSEITQGLKAIAKELDIPVIALSQLSRQVESREDKRPQLSDLRESGSIEQD
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
ADVVMFVYRGEYYKEREKPGEENLEAMTKWQQDMEHLHGKAEVIIGKQRHGPVGAVELAF
CCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEE
EGRFTRFGNLAQPRHGAPEGY
CCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA