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The map label for this gene is pgk

Identifier: 89054185

GI number: 89054185

Start: 1676398

End: 1677588

Strand: Reverse

Name: pgk

Synonym: Jann_1694

Alternate gene names: 89054185

Gene position: 1677588-1676398 (Counterclockwise)

Preceding gene: 89054186

Following gene: 89054184

Centisome position: 38.85

GC content: 65.58

Gene sequence:

>1191_bases
ATGCGCTTTAACACCCTCGACGACATGGATCTTGCGGGCAAACTCGTGCTGACCCGCGTGGATATCAACGTGCCGGTGGA
TGGTGACACGGTCACCGACGCCACCCGGATCACGCGCATCGTGCCGACGATCAAGGATATTCTCGCCAAAGGCGGTCGCC
CCGTCATGCTCGCCCATTTCGGGCGCCCCAAAGGCGCATATGTCCCGGAGATGAGCCTGCGCGTCACCGTTCCGGCCCTG
GAACAGGCCCTCGGCCAACCCGTCAATTTCATCGAACGGCCGGACCGTGCCACCCTCGACGCCCTGCCCGAGGGCACGAT
CACCTTGATCGAAAACACCCGCTTTTCCGCCATGGAAGAGGCGAATGACCCGAAGATGGCGGGCTTTCTCGCCTCCTTGG
GCGATGTCTATTGCAACGACGCCTTCTCCGCCGCGCACCGGGCCCATGCCTCCACGGAAGGGGTCGCGCGGCTGCTGCCC
TCCTGCGCCGGGCGTTTGATGGAGGCGGAGTTAAGCGCGCTGGAGGCGGCCCTTGGTGCGCCGCAGCGGCCCGTGGTGGC
CGTGGTGGGCGGGGCCAAAGTGTCCACGAAGCTGGATCTGCTTGGCAACCTGATTGAAAAGGTCGACACGATCATCATCG
GCGGCGGCATGGCCAACACGTTTCTGGCCGCGCAAGGCGTCCAGGTTGGCACGTCGCTATGCGAACACGATCTGGGCGAC
ACCGCACGCGCCATCATGCAGAAGGCGGCCGCCGCCAATTGCACCCTGATCCTGCCCGGTGACATTGTTGTTGCGGCCGA
GTTCAAGGCCGGGGCCGCCAACGCGGTCTATGACGTGGACGCCTGCCCCGCCGACCAGATGATCCTCGATGCCGGGCCAA
AGACCGTGGAGACCATCGCCGCGACGTTCAACACCGCCAAGACAGTGATCTGGAACGGCCCCCTGGGCGCGTTTGAAATC
GCACCCTTTGACGCGGCCACGGTCACTGCGGCCCGCGCCGCCGCAGAGGAAACGCAGGCGGGCAAGCTGATTTCCGTGGC
GGGCGGTGGCGACACGGTCGCGGCCCTCAACCACGCGGGCGTGGCTGATCAGTTCACCTATATCTCCACCGCAGGCGGCG
CGTTTCTGGAATGGATGGAGGGCAAGACCCTGCCCGGGGTCGCGGCACTGGAGGCGGCAAAACCCGCCTGA

Upstream 100 bases:

>100_bases
CCGTTCCTGAAATATCCCCGCCGGAGGCATAAATCTCTTTTGGCGCACCCGAAATCCGTGGCACCACGATCAGGACCCCA
CATTGTCAGGAGACACCCCC

Downstream 100 bases:

>100_bases
TAGCGCAACCATGATGGACGCCCAAAGGCCCGCGGCCTAAACCCTCCGCAACCCCGAAACATGAGGAGGCACCGATGGCC
AACGCAGAGCAGATCGCACA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRFNTLDDMDLAGKLVLTRVDINVPVDGDTVTDATRITRIVPTIKDILAKGGRPVMLAHFGRPKGAYVPEMSLRVTVPAL
EQALGQPVNFIERPDRATLDALPEGTITLIENTRFSAMEEANDPKMAGFLASLGDVYCNDAFSAAHRAHASTEGVARLLP
SCAGRLMEAELSALEAALGAPQRPVVAVVGGAKVSTKLDLLGNLIEKVDTIIIGGGMANTFLAAQGVQVGTSLCEHDLGD
TARAIMQKAAAANCTLILPGDIVVAAEFKAGAANAVYDVDACPADQMILDAGPKTVETIAATFNTAKTVIWNGPLGAFEI
APFDAATVTAARAAAEETQAGKLISVAGGGDTVAALNHAGVADQFTYISTAGGAFLEWMEGKTLPGVAALEAAKPA

Sequences:

>Translated_396_residues
MRFNTLDDMDLAGKLVLTRVDINVPVDGDTVTDATRITRIVPTIKDILAKGGRPVMLAHFGRPKGAYVPEMSLRVTVPAL
EQALGQPVNFIERPDRATLDALPEGTITLIENTRFSAMEEANDPKMAGFLASLGDVYCNDAFSAAHRAHASTEGVARLLP
SCAGRLMEAELSALEAALGAPQRPVVAVVGGAKVSTKLDLLGNLIEKVDTIIIGGGMANTFLAAQGVQVGTSLCEHDLGD
TARAIMQKAAAANCTLILPGDIVVAAEFKAGAANAVYDVDACPADQMILDAGPKTVETIAATFNTAKTVIWNGPLGAFEI
APFDAATVTAARAAAEETQAGKLISVAGGGDTVAALNHAGVADQFTYISTAGGAFLEWMEGKTLPGVAALEAAKPA
>Mature_396_residues
MRFNTLDDMDLAGKLVLTRVDINVPVDGDTVTDATRITRIVPTIKDILAKGGRPVMLAHFGRPKGAYVPEMSLRVTVPAL
EQALGQPVNFIERPDRATLDALPEGTITLIENTRFSAMEEANDPKMAGFLASLGDVYCNDAFSAAHRAHASTEGVARLLP
SCAGRLMEAELSALEAALGAPQRPVVAVVGGAKVSTKLDLLGNLIEKVDTIIIGGGMANTFLAAQGVQVGTSLCEHDLGD
TARAIMQKAAAANCTLILPGDIVVAAEFKAGAANAVYDVDACPADQMILDAGPKTVETIAATFNTAKTVIWNGPLGAFEI
APFDAATVTAARAAAEETQAGKLISVAGGGDTVAALNHAGVADQFTYISTAGGAFLEWMEGKTLPGVAALEAAKPA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=39.3643031784841, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=38.2926829268293, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=43.7974683544304, Blast_Score=303, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=38.5922330097087, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=40.1459854014599, Blast_Score=269, Evalue=7e-73,
Organism=Drosophila melanogaster, GI17136394, Length=418, Percent_Identity=39.9521531100478, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=38.2566585956416, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_JANSC (Q28RQ1)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509636.1
- ProteinModelPortal:   Q28RQ1
- SMR:   Q28RQ1
- STRING:   Q28RQ1
- GeneID:   3934142
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1694
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   JSP290400:JANN_1694-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40934; Mature: 40934

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 197-197 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNTLDDMDLAGKLVLTRVDINVPVDGDTVTDATRITRIVPTIKDILAKGGRPVMLAHF
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GRPKGAYVPEMSLRVTVPALEQALGQPVNFIERPDRATLDALPEGTITLIENTRFSAMEE
CCCCCCCCCCCEEEEECHHHHHHCCCCCHHHCCCCCCHHHCCCCCCEEEEECCCHHHHHH
ANDPKMAGFLASLGDVYCNDAFSAAHRAHASTEGVARLLPSCAGRLMEAELSALEAALGA
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQRPVVAVVGGAKVSTKLDLLGNLIEKVDTIIIGGGMANTFLAAQGVQVGTSLCEHDLGD
CCCCEEEEECCCCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCH
TARAIMQKAAAANCTLILPGDIVVAAEFKAGAANAVYDVDACPADQMILDAGPKTVETIA
HHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCEEECCCCCCCCEEECCCCHHHHHHH
ATFNTAKTVIWNGPLGAFEIAPFDAATVTAARAAAEETQAGKLISVAGGGDTVAALNHAG
HHHCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCC
VADQFTYISTAGGAFLEWMEGKTLPGVAALEAAKPA
CCCCEEEEECCCCHHHHHHCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MRFNTLDDMDLAGKLVLTRVDINVPVDGDTVTDATRITRIVPTIKDILAKGGRPVMLAHF
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GRPKGAYVPEMSLRVTVPALEQALGQPVNFIERPDRATLDALPEGTITLIENTRFSAMEE
CCCCCCCCCCCEEEEECHHHHHHCCCCCHHHCCCCCCHHHCCCCCCEEEEECCCHHHHHH
ANDPKMAGFLASLGDVYCNDAFSAAHRAHASTEGVARLLPSCAGRLMEAELSALEAALGA
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQRPVVAVVGGAKVSTKLDLLGNLIEKVDTIIIGGGMANTFLAAQGVQVGTSLCEHDLGD
CCCCEEEEECCCCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCH
TARAIMQKAAAANCTLILPGDIVVAAEFKAGAANAVYDVDACPADQMILDAGPKTVETIA
HHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCEEECCCCCCCCEEECCCCHHHHHHH
ATFNTAKTVIWNGPLGAFEIAPFDAATVTAARAAAEETQAGKLISVAGGGDTVAALNHAG
HHHCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCC
VADQFTYISTAGGAFLEWMEGKTLPGVAALEAAKPA
CCCCEEEEECCCCHHHHHHCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA