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The map label for this gene is siaP [H]

Identifier: 89053840

GI number: 89053840

Start: 1313539

End: 1314684

Strand: Direct

Name: siaP [H]

Synonym: Jann_1349

Alternate gene names: 89053840

Gene position: 1313539-1314684 (Clockwise)

Preceding gene: 89053839

Following gene: 89053841

Centisome position: 30.42

GC content: 62.04

Gene sequence:

>1146_bases
TTGGATCACGACACTCAAGAGCTGAAGTCGGCAGAGCGCCGCAATTTCCTGAAGCTGACCGGCGCAGGTGCATTCACCGC
CGCCATGGTCGCCGGTGGCGCGGGCATGCTGTGGTCGACTGAGGCCGCAGCCCAGACCCAACAGGAAGAGGCCGCGCGCG
AAGCCGCAGCCGAGCACATTATGACGCTGGCCACGGCCTATGTTCTGGGTGCCTCGCGCAGCTATCCGATCATGCAACTG
GACCTGAAAGAGAATATCCAGAACATGACCAACGGCAAAGTCTATGTGCGTCTCGCGCCGGGCGGTCAGTTGGGGGCAGG
GGGCGCTTTGGCTCAGGCCGTGCAATCCGGCACGATCCAATGCGCGCAGCACTCGCTGGCCAACTTCGCACCGTTTGCCT
CCGCCGTTGATCTGATCAACCTGCCGTATTTCTGCGGCTCCAACCAGCGCTTCACCAACCTGACCTCGTCGGATGCTTGG
AAAGAAGAAGTGGACCCGCGTATCGGAGAGTCTGGTTTCAAAGCCCTGATGTATATCGTCATCGACCCCCGCGTCGTGGC
CGTGCGCCAGGGCGGCGACGCGATCATCACGCCCGCCGACATGGAAGGCGTCAAGTTCCGCGTTCCCGGATCTGCAATGT
TGCAGCAATACTACCGTATGGTCGGCGCCAACCCGACGCCCGTGGCCTGGGGTGAGACACCGTCCGCGATCCGCCAGGGT
GTGGCCGACGCACTCGACCCGTCCGTGGGCGCATTGCACGTCTTCGGCTTTGGTGAAATCCTCAGCCATGTGACCTTCAC
GCAGGCCGTGCCCGACAGCCAAGTCTACTCGGTCAATCTGGAATGGTTCAACTCGCTGCCTGCCGACGTGCAGGAAGGCA
TTGAGTTTGCAGGCGAAGTCACCCAGCAGCAGAACCTGGCCAAAGTGCCCTCGGCGCGGTCCTACGCGATGTCCCAATTG
TCGGCAAATGGGGTGGAGTTCCACTCCCTGTCGGACGATCAACTGGCCGAGTGGCAGTCCGTGGGCGGCTATCAATTGCC
CGATTGGGACGAGTTCAAAGTCGATCTGGCCGGCTCCATGGAGACCTTCGCCCGTCTTGAGGAAGCCGCAGGCACCGCCA
GCCGCTACTACGTCCACGACGCCTAA

Upstream 100 bases:

>100_bases
ATTTTGTTCGTCATCGGGATCCTGAAAAAAGAAGCAGACGCCAAGGCAGAGGCCGCAGCAGCCCAAGCCCGCGTCGACGA
CGTCCAGGGAGGACACTGAT

Downstream 100 bases:

>100_bases
CACCGAAGGGCCTCGCCGAATACCGGCGGGGCCCTTTTTCGGTCAAGACCGATCCAACACGCCAATGCCATTCCACCGCA
CGCCCGGCCCATGATTGCAG

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: Extracytoplasmic solute receptor protein siaP; N-acetylneuraminic-binding protein; Neu5Ac-binding protein [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MDHDTQELKSAERRNFLKLTGAGAFTAAMVAGGAGMLWSTEAAAQTQQEEAAREAAAEHIMTLATAYVLGASRSYPIMQL
DLKENIQNMTNGKVYVRLAPGGQLGAGGALAQAVQSGTIQCAQHSLANFAPFASAVDLINLPYFCGSNQRFTNLTSSDAW
KEEVDPRIGESGFKALMYIVIDPRVVAVRQGGDAIITPADMEGVKFRVPGSAMLQQYYRMVGANPTPVAWGETPSAIRQG
VADALDPSVGALHVFGFGEILSHVTFTQAVPDSQVYSVNLEWFNSLPADVQEGIEFAGEVTQQQNLAKVPSARSYAMSQL
SANGVEFHSLSDDQLAEWQSVGGYQLPDWDEFKVDLAGSMETFARLEEAAGTASRYYVHDA

Sequences:

>Translated_381_residues
MDHDTQELKSAERRNFLKLTGAGAFTAAMVAGGAGMLWSTEAAAQTQQEEAAREAAAEHIMTLATAYVLGASRSYPIMQL
DLKENIQNMTNGKVYVRLAPGGQLGAGGALAQAVQSGTIQCAQHSLANFAPFASAVDLINLPYFCGSNQRFTNLTSSDAW
KEEVDPRIGESGFKALMYIVIDPRVVAVRQGGDAIITPADMEGVKFRVPGSAMLQQYYRMVGANPTPVAWGETPSAIRQG
VADALDPSVGALHVFGFGEILSHVTFTQAVPDSQVYSVNLEWFNSLPADVQEGIEFAGEVTQQQNLAKVPSARSYAMSQL
SANGVEFHSLSDDQLAEWQSVGGYQLPDWDEFKVDLAGSMETFARLEEAAGTASRYYVHDA
>Mature_381_residues
MDHDTQELKSAERRNFLKLTGAGAFTAAMVAGGAGMLWSTEAAAQTQQEEAAREAAAEHIMTLATAYVLGASRSYPIMQL
DLKENIQNMTNGKVYVRLAPGGQLGAGGALAQAVQSGTIQCAQHSLANFAPFASAVDLINLPYFCGSNQRFTNLTSSDAW
KEEVDPRIGESGFKALMYIVIDPRVVAVRQGGDAIITPADMEGVKFRVPGSAMLQQYYRMVGANPTPVAWGETPSAIRQG
VADALDPSVGALHVFGFGEILSHVTFTQAVPDSQVYSVNLEWFNSLPADVQEGIEFAGEVTQQQNLAKVPSARSYAMSQL
SANGVEFHSLSDDQLAEWQSVGGYQLPDWDEFKVDLAGSMETFARLEEAAGTASRYYVHDA

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid. This protein specifically binds sialic acid [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=209, Percent_Identity=23.444976076555, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 40960; Mature: 40960

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHDTQELKSAERRNFLKLTGAGAFTAAMVAGGAGMLWSTEAAAQTQQEEAAREAAAEHI
CCCCHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHH
MTLATAYVLGASRSYPIMQLDLKENIQNMTNGKVYVRLAPGGQLGAGGALAQAVQSGTIQ
HHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCHH
CAQHSLANFAPFASAVDLINLPYFCGSNQRFTNLTSSDAWKEEVDPRIGESGFKALMYIV
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCHHHEEEEEE
IDPRVVAVRQGGDAIITPADMEGVKFRVPGSAMLQQYYRMVGANPTPVAWGETPSAIRQG
ECCEEEEEECCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VADALDPSVGALHVFGFGEILSHVTFTQAVPDSQVYSVNLEWFNSLPADVQEGIEFAGEV
HHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHH
TQQQNLAKVPSARSYAMSQLSANGVEFHSLSDDQLAEWQSVGGYQLPDWDEFKVDLAGSM
HHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEECCCH
ETFARLEEAAGTASRYYVHDA
HHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MDHDTQELKSAERRNFLKLTGAGAFTAAMVAGGAGMLWSTEAAAQTQQEEAAREAAAEHI
CCCCHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHH
MTLATAYVLGASRSYPIMQLDLKENIQNMTNGKVYVRLAPGGQLGAGGALAQAVQSGTIQ
HHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCHH
CAQHSLANFAPFASAVDLINLPYFCGSNQRFTNLTSSDAWKEEVDPRIGESGFKALMYIV
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCHHHEEEEEE
IDPRVVAVRQGGDAIITPADMEGVKFRVPGSAMLQQYYRMVGANPTPVAWGETPSAIRQG
ECCEEEEEECCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VADALDPSVGALHVFGFGEILSHVTFTQAVPDSQVYSVNLEWFNSLPADVQEGIEFAGEV
HHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHH
TQQQNLAKVPSARSYAMSQLSANGVEFHSLSDDQLAEWQSVGGYQLPDWDEFKVDLAGSM
HHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEECCCH
ETFARLEEAAGTASRYYVHDA
HHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA