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The map label for this gene is hisJ [H]

Identifier: 89053401

GI number: 89053401

Start: 865519

End: 866319

Strand: Direct

Name: hisJ [H]

Synonym: Jann_0910

Alternate gene names: 89053401

Gene position: 865519-866319 (Clockwise)

Preceding gene: 89053400

Following gene: 89053402

Centisome position: 20.04

GC content: 62.42

Gene sequence:

>801_bases
ATGACTCGTTTTTTCGCCGCCGCCTTGGCCGCCCCTCTGGCCGCTTTGATGCCTTTTGCCGCCGCCGCCGATGGGCATTT
GCCTGATCTGGGCGGGCAGGAGGTCGTGATCGTCACGGAAAACGCCTATCCGCCGTTGCAATTCGTGGACCCCGTGAGTG
GCCTTGCGATTGGGTGGGAATATGACGCGGTGGAGATCCTGGCGGAGCGCCTGAACTTCACGCCCGTCATCGAGAATATC
AGCTGGGACGCGATGATCCCCGCCGTCTCGGGCGGGCAGTACGATATGGGCATGACGGGCATCACCATCCGCGAAGATCG
CGATGAGCTGGTCGATTTCTCCGTCCCCTACATGGTCAGCCAGATGCGCATGCTGGTGGCCGCCGATGAGACGCGCTTTG
ACGATGCCGAGAGCTTTGCCGCAGACGAGGATCTGTTGATCGGGACGCAGGCGGGCACCACGCCGTTCTATGTGGGCGTT
TACGATGTGCTGGATGGGGATGAGGCCAATCCGCGCCTTGTTCTGCTGGAGACGATCCCGGCGGCGATCCAGGCGCTGAT
CACCGGCGATGTGGATATGGTGCTGGCCGATAGCGCGGGTGGGCAGGGCTATGTCAGCGCCAATCCCGACCGGTTGCAGA
TGATCGGGGAGCCGCTTGGCACCGAGGAATTTGGCTTCATCTTCCCCAATGACAGCGAGCTGACGGCGGCGATCAACGCC
GGTCTGGACAGCATGCGCGAGGATGGGACCCTGGAGGAGTTGGACCAGCGCTGGTTTGTCGATTACGCGCTCGGCCAGTA
A

Upstream 100 bases:

>100_bases
TTTGCGACGGGGTTTGACGCGGATTGGACGAAAGGCCGGGTCAGGGCTGGACGGCGGGGCCACGCGCGCCATACTGGCGA
AAACCAACCGGGAGCAAGAT

Downstream 100 bases:

>100_bases
CGAAACAAAGGTGCGCCGGGGGCTCTCGGCGCGCCGTCCCTGATGCTTTCCCGCTCCCCCCAGGACCAGGATTTCCCGTG
GTGGCTTGTCGCTTTGGGCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTRFFAAALAAPLAALMPFAAAADGHLPDLGGQEVVIVTENAYPPLQFVDPVSGLAIGWEYDAVEILAERLNFTPVIENI
SWDAMIPAVSGGQYDMGMTGITIREDRDELVDFSVPYMVSQMRMLVAADETRFDDAESFAADEDLLIGTQAGTTPFYVGV
YDVLDGDEANPRLVLLETIPAAIQALITGDVDMVLADSAGGQGYVSANPDRLQMIGEPLGTEEFGFIFPNDSELTAAINA
GLDSMREDGTLEELDQRWFVDYALGQ

Sequences:

>Translated_266_residues
MTRFFAAALAAPLAALMPFAAAADGHLPDLGGQEVVIVTENAYPPLQFVDPVSGLAIGWEYDAVEILAERLNFTPVIENI
SWDAMIPAVSGGQYDMGMTGITIREDRDELVDFSVPYMVSQMRMLVAADETRFDDAESFAADEDLLIGTQAGTTPFYVGV
YDVLDGDEANPRLVLLETIPAAIQALITGDVDMVLADSAGGQGYVSANPDRLQMIGEPLGTEEFGFIFPNDSELTAAINA
GLDSMREDGTLEELDQRWFVDYALGQ
>Mature_265_residues
TRFFAAALAAPLAALMPFAAAADGHLPDLGGQEVVIVTENAYPPLQFVDPVSGLAIGWEYDAVEILAERLNFTPVIENIS
WDAMIPAVSGGQYDMGMTGITIREDRDELVDFSVPYMVSQMRMLVAADETRFDDAESFAADEDLLIGTQAGTTPFYVGVY
DVLDGDEANPRLVLLETIPAAIQALITGDVDMVLADSAGGQGYVSANPDRLQMIGEPLGTEEFGFIFPNDSELTAAINAG
LDSMREDGTLEELDQRWFVDYALGQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=255, Percent_Identity=26.2745098039216, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=27.1317829457364, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787088, Length=241, Percent_Identity=22.4066390041494, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=22.0720720720721, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28686; Mature: 28555

Theoretical pI: Translated: 3.51; Mature: 3.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFFAAALAAPLAALMPFAAAADGHLPDLGGQEVVIVTENAYPPLQFVDPVSGLAIGWE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCEEECCC
YDAVEILAERLNFTPVIENISWDAMIPAVSGGQYDMGMTGITIREDRDELVDFSVPYMVS
HHHHHHHHHHCCCCEEECCCCCCEECCCCCCCCCCCCCCEEEEECCHHHHHCCCCHHHHH
QMRMLVAADETRFDDAESFAADEDLLIGTQAGTTPFYVGVYDVLDGDEANPRLVLLETIP
HHHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEHHH
AAIQALITGDVDMVLADSAGGQGYVSANPDRLQMIGEPLGTEEFGFIFPNDSELTAAINA
HHHHHHHCCCEEEEEEECCCCCEEECCCCHHHHHHCCCCCCCCCEEEECCCCCEEHHHHH
GLDSMREDGTLEELDQRWFVDYALGQ
CHHHHHCCCCHHHHHHHHEHEECCCC
>Mature Secondary Structure 
TRFFAAALAAPLAALMPFAAAADGHLPDLGGQEVVIVTENAYPPLQFVDPVSGLAIGWE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCEEECCC
YDAVEILAERLNFTPVIENISWDAMIPAVSGGQYDMGMTGITIREDRDELVDFSVPYMVS
HHHHHHHHHHCCCCEEECCCCCCEECCCCCCCCCCCCCCEEEEECCHHHHHCCCCHHHHH
QMRMLVAADETRFDDAESFAADEDLLIGTQAGTTPFYVGVYDVLDGDEANPRLVLLETIP
HHHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEHHH
AAIQALITGDVDMVLADSAGGQGYVSANPDRLQMIGEPLGTEEFGFIFPNDSELTAAINA
HHHHHHHCCCEEEEEEECCCCCEEECCCCHHHHHHCCCCCCCCCEEEECCCCCEEHHHHH
GLDSMREDGTLEELDQRWFVDYALGQ
CHHHHHCCCCHHHHHHHHEHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]