Click here to switch to the map view.

The map label for this gene is rpoH2

Identifier: 89053397

GI number: 89053397

Start: 861536

End: 862414

Strand: Direct

Name: rpoH2

Synonym: Jann_0906

Alternate gene names: 89053397

Gene position: 861536-862414 (Clockwise)

Preceding gene: 89053392

Following gene: 89053398

Centisome position: 19.95

GC content: 60.52

Gene sequence:

>879_bases
ATGGCATTTGATATGGGAGATCAGTCTCTGTCCCGTCGCGCGATGAAGGCAGAGTTGTTGGACGCAGAAACCGAGTTGCG
CCTTGCCTATGCGTGGCGCGATGACCGCTGCGAGGAAAGCCTGCACCGGCTGGTGACGGCCTATATGCGCCTTGCGATTT
CCATGGCCGCCAAGTTCAAACGCTATGGCGCGCCGATGAACGACCTGATCCAGGAAGCCGGTCTGGGCCTGATGAAGGCC
GCCGACAAGTTCGACCCCGACCGTGGCGTGCGGTTTTCGACCTATGCCGTCTGGTGGATCAAGGCGAGCATTCAGGATCA
TGTGATGCGCAACTGGTCGATGGTGCGCACGGGCTCCACCTCCTCGCAAAAGTCCCTGTTTTTCAACATGCGCCGGGTCC
AGGCGCGGTTGGAGCGGGAGGCCATGAAGTCGGGCGACACTCTGGATAAACATCAGCTGCGCCAGATGATCGCGACCGAA
GTAGGGGTCCCCCTTCATGACGTCGAGATGATGGAAGGACGCCTTGCGGGTTCGGATTATTCGCTGAATGCGACCCAATC
CGCCGAGGATGAGGGCCGCGAGTGGATCGATACGCTGGAAGATGATGGCGAGCAGGCTGAGCACATTGTTGAGCATTTCC
ACGACACCGCGCAGTTGCGTGACTGGCTGATCGCGTCGATGAATGCGCTGAATGACCGTGAGAGGTTCATCGTGCGCGAG
CGCAAGTTGCGGGAAGATCCCCGGACGCTGGAAAGCCTGGGGACGGAGCTTGGCCTGTCCAAGGAGCGCGTGCGCCAGTT
GGAAGCCGCGGCGTTCGGCAAGATGCGCAAGACGCTGGAGGCGCAGTCGAAGGAGGTCCGGTCATTCCTCGCCGTTTGA

Upstream 100 bases:

>100_bases
ATGTCCCAACGAGTGATCCAAACGATGGATGCCTGCGTAGCATATATGAACACGCGGTAAACGCTTGTCGAATAACTGAG
TTAGAACACCGGGGGTCGTT

Downstream 100 bases:

>100_bases
TATTGCACGCGCCGTCGGGGCTTCTGGCCGGGTTGTTTCTGCTGACGCTGGCCGTCTCGCCGCCGCCGGGTACTGGATTT
GCGGTGCTCGGGATCGACTG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAFDMGDQSLSRRAMKAELLDAETELRLAYAWRDDRCEESLHRLVTAYMRLAISMAAKFKRYGAPMNDLIQEAGLGLMKA
ADKFDPDRGVRFSTYAVWWIKASIQDHVMRNWSMVRTGSTSSQKSLFFNMRRVQARLEREAMKSGDTLDKHQLRQMIATE
VGVPLHDVEMMEGRLAGSDYSLNATQSAEDEGREWIDTLEDDGEQAEHIVEHFHDTAQLRDWLIASMNALNDRERFIVRE
RKLREDPRTLESLGTELGLSKERVRQLEAAAFGKMRKTLEAQSKEVRSFLAV

Sequences:

>Translated_292_residues
MAFDMGDQSLSRRAMKAELLDAETELRLAYAWRDDRCEESLHRLVTAYMRLAISMAAKFKRYGAPMNDLIQEAGLGLMKA
ADKFDPDRGVRFSTYAVWWIKASIQDHVMRNWSMVRTGSTSSQKSLFFNMRRVQARLEREAMKSGDTLDKHQLRQMIATE
VGVPLHDVEMMEGRLAGSDYSLNATQSAEDEGREWIDTLEDDGEQAEHIVEHFHDTAQLRDWLIASMNALNDRERFIVRE
RKLREDPRTLESLGTELGLSKERVRQLEAAAFGKMRKTLEAQSKEVRSFLAV
>Mature_291_residues
AFDMGDQSLSRRAMKAELLDAETELRLAYAWRDDRCEESLHRLVTAYMRLAISMAAKFKRYGAPMNDLIQEAGLGLMKAA
DKFDPDRGVRFSTYAVWWIKASIQDHVMRNWSMVRTGSTSSQKSLFFNMRRVQARLEREAMKSGDTLDKHQLRQMIATEV
GVPLHDVEMMEGRLAGSDYSLNATQSAEDEGREWIDTLEDDGEQAEHIVEHFHDTAQLRDWLIASMNALNDRERFIVRER
KLREDPRTLESLGTELGLSKERVRQLEAAAFGKMRKTLEAQSKEVRSFLAV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=259, Percent_Identity=35.5212355212355, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=30.2491103202847, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=29.1828793774319, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33611; Mature: 33479

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDMGDQSLSRRAMKAELLDAETELRLAYAWRDDRCEESLHRLVTAYMRLAISMAAKFK
CCCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGAPMNDLIQEAGLGLMKAADKFDPDRGVRFSTYAVWWIKASIQDHVMRNWSMVRTGST
HHCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEHEEEEEEHHHHHHHHHHCCHHEECCCC
SSQKSLFFNMRRVQARLEREAMKSGDTLDKHQLRQMIATEVGVPLHDVEMMEGRLAGSDY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
SLNATQSAEDEGREWIDTLEDDGEQAEHIVEHFHDTAQLRDWLIASMNALNDRERFIVRE
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RKLREDPRTLESLGTELGLSKERVRQLEAAAFGKMRKTLEAQSKEVRSFLAV
HHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFDMGDQSLSRRAMKAELLDAETELRLAYAWRDDRCEESLHRLVTAYMRLAISMAAKFK
CCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGAPMNDLIQEAGLGLMKAADKFDPDRGVRFSTYAVWWIKASIQDHVMRNWSMVRTGST
HHCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEHEEEEEEHHHHHHHHHHCCHHEECCCC
SSQKSLFFNMRRVQARLEREAMKSGDTLDKHQLRQMIATEVGVPLHDVEMMEGRLAGSDY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
SLNATQSAEDEGREWIDTLEDDGEQAEHIVEHFHDTAQLRDWLIASMNALNDRERFIVRE
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RKLREDPRTLESLGTELGLSKERVRQLEAAAFGKMRKTLEAQSKEVRSFLAV
HHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]