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The map label for this gene is gshB [H]

Identifier: 89053392

GI number: 89053392

Start: 856984

End: 857928

Strand: Direct

Name: gshB [H]

Synonym: Jann_0901

Alternate gene names: 89053392

Gene position: 856984-857928 (Clockwise)

Preceding gene: 89053391

Following gene: 89053397

Centisome position: 19.85

GC content: 62.12

Gene sequence:

>945_bases
ATGTTGATCGCATTTCAGATGGATCCGATCGAAGACGTGGACATCGACGCGGATTCGTCTTTTCGATTGGCGGAAGAGGC
GCAGGCGCGCGGGCATGATCTGATGGTCTATACGCCCGACGATCTGTTCTACCGTGAAGACAAGGTCTGCGCTCGGGCCC
GCCGCATCAGCGTGCAGCGCGTTAAAGGCGACCACGTGGTAGCGCAGGCCCCTGAGATTGTGGATCTGGCGGAGGTCGAT
GTGATCTGGCTGCGTCAGGACCCGCCTTTTGACATGGGCTATATCACGACGACGCATCTGCTGGACCGTCTGCGCGACAA
GACGCTGGTGGTGAATGATCCGTTCTGGGTGCGCAATTCGCCCGAAAAGCTGATGGTCCTGGACTACCCCGACCTCACAC
CGCCCACGATGATCGCCCGCGACATCGACATGCTGAAGGCGTTCAAGGAGGAGCACGGGGACGTGATCCTCAAGCCGCTC
TACGGCAATGGCGGCGCGGGCGTGTTTCGCCTGACGCCCGAGGACCGCAACCTGACGTCGCTCCACGAGTTGTTCACCGG
CATCAACCGGGAGCCGCTTATCATGCAGAAGTTCCTTCCCGATGTCTCCGCTGGCGATAAACGCGTGATCCTGGTGGATG
GGGAGCCCGTGGGCGGCATCAATCGCGTCCCGGCCAAGGGCGAGGTCCGCTCCAACATGCATGTCGGCGGACGGCCCGAG
GCCACGGCCCTGACCGAGCGGGAGAGAGAAATCTGCGCCATCATCGGCCCGCGCCTGAAGGAGATGGGACAAGTGTTTGT
GGGCATTGACGTGATCGGAGGCTGGCTGACCGAGATCAACGTGACCTCACCCACCGGCATTCAGGAATTGGAGCGGTTTG
ACGGCGTCAACATCGCCGCCCGGATCTGGGACGCCATCGAGGACCGCCACGCGGCGATGCCCTAG

Upstream 100 bases:

>100_bases
GCTCGACGTCATCGTGAACGCATTAGCCGCCTGATCCCCATTGCGCCGGACCCGGCGTCACGCCAAGTAAGGGGCTTGAG
TATGACCGAAGGGGCCAGGC

Downstream 100 bases:

>100_bases
ACGTCGCGCGCACCGATCAGCCGATACCCCACCGCCTCGGCGATATGGGGCACGCTGACCGTCTCCACCCCATCCAGATC
GGCAATGGTGCGCGCCAGTC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLIAFQMDPIEDVDIDADSSFRLAEEAQARGHDLMVYTPDDLFYREDKVCARARRISVQRVKGDHVVAQAPEIVDLAEVD
VIWLRQDPPFDMGYITTTHLLDRLRDKTLVVNDPFWVRNSPEKLMVLDYPDLTPPTMIARDIDMLKAFKEEHGDVILKPL
YGNGGAGVFRLTPEDRNLTSLHELFTGINREPLIMQKFLPDVSAGDKRVILVDGEPVGGINRVPAKGEVRSNMHVGGRPE
ATALTEREREICAIIGPRLKEMGQVFVGIDVIGGWLTEINVTSPTGIQELERFDGVNIAARIWDAIEDRHAAMP

Sequences:

>Translated_314_residues
MLIAFQMDPIEDVDIDADSSFRLAEEAQARGHDLMVYTPDDLFYREDKVCARARRISVQRVKGDHVVAQAPEIVDLAEVD
VIWLRQDPPFDMGYITTTHLLDRLRDKTLVVNDPFWVRNSPEKLMVLDYPDLTPPTMIARDIDMLKAFKEEHGDVILKPL
YGNGGAGVFRLTPEDRNLTSLHELFTGINREPLIMQKFLPDVSAGDKRVILVDGEPVGGINRVPAKGEVRSNMHVGGRPE
ATALTEREREICAIIGPRLKEMGQVFVGIDVIGGWLTEINVTSPTGIQELERFDGVNIAARIWDAIEDRHAAMP
>Mature_314_residues
MLIAFQMDPIEDVDIDADSSFRLAEEAQARGHDLMVYTPDDLFYREDKVCARARRISVQRVKGDHVVAQAPEIVDLAEVD
VIWLRQDPPFDMGYITTTHLLDRLRDKTLVVNDPFWVRNSPEKLMVLDYPDLTPPTMIARDIDMLKAFKEEHGDVILKPL
YGNGGAGVFRLTPEDRNLTSLHELFTGINREPLIMQKFLPDVSAGDKRVILVDGEPVGGINRVPAKGEVRSNMHVGGRPE
ATALTEREREICAIIGPRLKEMGQVFVGIDVIGGWLTEINVTSPTGIQELERFDGVNIAARIWDAIEDRHAAMP

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=305, Percent_Identity=44.2622950819672, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1787076, Length=161, Percent_Identity=32.2981366459627, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35246; Mature: 35246

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAFQMDPIEDVDIDADSSFRLAEEAQARGHDLMVYTPDDLFYREDKVCARARRISVQR
CEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCHHEECCHHHHHHHHHHEEEE
VKGDHVVAQAPEIVDLAEVDVIWLRQDPPFDMGYITTTHLLDRLRDKTLVVNDPFWVRNS
ECCCEEEECCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCEEECCC
PEKLMVLDYPDLTPPTMIARDIDMLKAFKEEHGDVILKPLYGNGGAGVFRLTPEDRNLTS
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCHHH
LHELFTGINREPLIMQKFLPDVSAGDKRVILVDGEPVGGINRVPAKGEVRSNMHVGGRPE
HHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
ATALTEREREICAIIGPRLKEMGQVFVGIDVIGGWLTEINVTSPTGIQELERFDGVNIAA
HHHHHHHHHHHHHHHCHHHHHHCCEEEEEEHHCCEEEEEEECCCCCHHHHHHHCCCCHHH
RIWDAIEDRHAAMP
HHHHHHHHHHCCCC
>Mature Secondary Structure
MLIAFQMDPIEDVDIDADSSFRLAEEAQARGHDLMVYTPDDLFYREDKVCARARRISVQR
CEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCHHEECCHHHHHHHHHHEEEE
VKGDHVVAQAPEIVDLAEVDVIWLRQDPPFDMGYITTTHLLDRLRDKTLVVNDPFWVRNS
ECCCEEEECCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCEEECCC
PEKLMVLDYPDLTPPTMIARDIDMLKAFKEEHGDVILKPLYGNGGAGVFRLTPEDRNLTS
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCHHH
LHELFTGINREPLIMQKFLPDVSAGDKRVILVDGEPVGGINRVPAKGEVRSNMHVGGRPE
HHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
ATALTEREREICAIIGPRLKEMGQVFVGIDVIGGWLTEINVTSPTGIQELERFDGVNIAA
HHHHHHHHHHHHHHHCHHHHHHCCEEEEEEHHCCEEEEEEECCCCCHHHHHHHCCCCHHH
RIWDAIEDRHAAMP
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]