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The map label for this gene is bioF [C]

Identifier: 88658397

GI number: 88658397

Start: 970501

End: 971604

Strand: Reverse

Name: bioF [C]

Synonym: ECH_0950

Alternate gene names: 88658397

Gene position: 971604-970501 (Counterclockwise)

Preceding gene: 88657749

Following gene: 88658056

Centisome position: 82.6

GC content: 31.7

Gene sequence:

>1104_bases
ATGTTGGATGAAATATTAAATCAAGAACTTATTAAATTAAAGGTTGATAATTTATATAGAGAACCTTCTACTGTAACTAG
ATCTGAAGTAGGTTGTTTGGTTTGTCAAAATAACGAGAGATTAGTTTCCTTCTCTTGTAATGATTATTTAGGGTTAATAG
GTCATCCTTTATTAAAAGAGTCAGCTATAAATGCGATAAATAATTATGGTGTAGGTGCTGGTGCCTCACGCATGGTTACA
GGGAATAATATCCTTTATCAATGCTTAGAAGATAAATTAGCAAAGTTATATCATACAGAAATGGCTTTAGTATTTAGTAG
TGGTTATTTAACAAATGTAGGTGTTATTTCTGCTTTAGTGCATAGACATGATATGATCATTTCTGACAAATTAGTACATT
CTTCTATTATAGATGGTATTAAACTTTCTTCTGCTAAGCATTATAGGTTTGAGCATAATGATTATGGACATTGTGAAGAT
ATATTAAAAAAATATAGGAGGTTACATAAGTACTGTTTTATTGTAGTTGAGCAAGTGTATAGTATGAATGGTGATATTGC
TCCAATTGATCAGTTAAAAAAATTGGCGGAAAAATATAGTGCGTGGCTTATTGTGGATTGTGCTCATGGATTTGGATTAA
TACCTTGTGCTAATTCAGATATATACATTGGTACTTTATCTAAAGCAGTTGGTGTTTTAGGTGGATATGTATGTGCATCG
GAGGTTGTTATAAAGTATATACAGAATAAAGCTAAAACTTTTATATATACGACTGCTCTACCTCCGATGGTTATTGCTGC
TGCGAATGCTGCGTTGGATATTATAAGTGCATCTGTTAATGATATACCTATTAAATTGGCTAAATTTTTTTGTAAAAATC
TGAATCTTGCTGAACCTAGTAGTCATATAGTACCATTAATTATGAAAAATATGAATAGCGTTTTACATGCACAGCAAGTG
TTAAGGGAAGCAGGTTTTCTTGTTGTGGCAATACGTCCTCCTACTGTACCGACTCCAAGACTGAGATTTGTATTTAGTGT
GAATCATAGCTTATCAGATATAGAGAAATTGTGTGAAGTTATAAAACGAAATAAGTTAGCCTAA

Upstream 100 bases:

>100_bases
TTTTTTTGTAGTGAGAACTTGTATTTGAGTGTATTTTGTCAAAAGTCCAAAATAGGGGTAGCTGATAAATATTAAGTTAC
AGTGCTGTGGAGTTTTTCAT

Downstream 100 bases:

>100_bases
ACAACTGCTATTGCATGAAAACATAATATTAGTATTTATCTTAGTATAAAAGTGATAAGTGGACCTGTTTATTATCATTA
AAAAGTAAAGCATGTTACTT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLDEILNQELIKLKVDNLYREPSTVTRSEVGCLVCQNNERLVSFSCNDYLGLIGHPLLKESAINAINNYGVGAGASRMVT
GNNILYQCLEDKLAKLYHTEMALVFSSGYLTNVGVISALVHRHDMIISDKLVHSSIIDGIKLSSAKHYRFEHNDYGHCED
ILKKYRRLHKYCFIVVEQVYSMNGDIAPIDQLKKLAEKYSAWLIVDCAHGFGLIPCANSDIYIGTLSKAVGVLGGYVCAS
EVVIKYIQNKAKTFIYTTALPPMVIAAANAALDIISASVNDIPIKLAKFFCKNLNLAEPSSHIVPLIMKNMNSVLHAQQV
LREAGFLVVAIRPPTVPTPRLRFVFSVNHSLSDIEKLCEVIKRNKLA

Sequences:

>Translated_367_residues
MLDEILNQELIKLKVDNLYREPSTVTRSEVGCLVCQNNERLVSFSCNDYLGLIGHPLLKESAINAINNYGVGAGASRMVT
GNNILYQCLEDKLAKLYHTEMALVFSSGYLTNVGVISALVHRHDMIISDKLVHSSIIDGIKLSSAKHYRFEHNDYGHCED
ILKKYRRLHKYCFIVVEQVYSMNGDIAPIDQLKKLAEKYSAWLIVDCAHGFGLIPCANSDIYIGTLSKAVGVLGGYVCAS
EVVIKYIQNKAKTFIYTTALPPMVIAAANAALDIISASVNDIPIKLAKFFCKNLNLAEPSSHIVPLIMKNMNSVLHAQQV
LREAGFLVVAIRPPTVPTPRLRFVFSVNHSLSDIEKLCEVIKRNKLA
>Mature_367_residues
MLDEILNQELIKLKVDNLYREPSTVTRSEVGCLVCQNNERLVSFSCNDYLGLIGHPLLKESAINAINNYGVGAGASRMVT
GNNILYQCLEDKLAKLYHTEMALVFSSGYLTNVGVISALVHRHDMIISDKLVHSSIIDGIKLSSAKHYRFEHNDYGHCED
ILKKYRRLHKYCFIVVEQVYSMNGDIAPIDQLKKLAEKYSAWLIVDCAHGFGLIPCANSDIYIGTLSKAVGVLGGYVCAS
EVVIKYIQNKAKTFIYTTALPPMVIAAANAALDIISASVNDIPIKLAKFFCKNLNLAEPSSHIVPLIMKNMNSVLHAQQV
LREAGFLVVAIRPPTVPTPRLRFVFSVNHSLSDIEKLCEVIKRNKLA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=383, Percent_Identity=28.1984334203655, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI284448556, Length=343, Percent_Identity=29.1545189504373, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI83977440, Length=392, Percent_Identity=30.1020408163265, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI83977444, Length=392, Percent_Identity=30.1020408163265, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI83977442, Length=392, Percent_Identity=30.1020408163265, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4502025, Length=367, Percent_Identity=28.6103542234332, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI40316939, Length=367, Percent_Identity=28.6103542234332, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4758668, Length=330, Percent_Identity=28.1818181818182, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI119220554, Length=330, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI5454084, Length=376, Percent_Identity=25, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786993, Length=360, Percent_Identity=33.6111111111111, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1790046, Length=381, Percent_Identity=28.6089238845144, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71994529, Length=382, Percent_Identity=27.2251308900524, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI32566772, Length=386, Percent_Identity=27.2020725388601, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17560914, Length=353, Percent_Identity=26.628895184136, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=353, Percent_Identity=26.628895184136, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982617, Length=266, Percent_Identity=25.5639097744361, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982625, Length=263, Percent_Identity=25.4752851711027, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320438, Length=402, Percent_Identity=33.8308457711443, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320267, Length=399, Percent_Identity=26.0651629072682, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323954, Length=288, Percent_Identity=25, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24662918, Length=379, Percent_Identity=27.1767810026385, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137420, Length=403, Percent_Identity=29.0322580645161, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24653280, Length=388, Percent_Identity=24.7422680412371, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653276, Length=388, Percent_Identity=24.7422680412371, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653278, Length=388, Percent_Identity=24.7422680412371, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17136286, Length=325, Percent_Identity=25.8461538461538, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 40739; Mature: 40739

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEILNQELIKLKVDNLYREPSTVTRSEVGCLVCQNNERLVSFSCNDYLGLIGHPLLKE
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCEEEEEECCCHHHHHCCHHHHH
SAINAINNYGVGAGASRMVTGNNILYQCLEDKLAKLYHTEMALVFSSGYLTNVGVISALV
HHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHH
HRHDMIISDKLVHSSIIDGIKLSSAKHYRFEHNDYGHCEDILKKYRRLHKYCFIVVEQVY
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SMNGDIAPIDQLKKLAEKYSAWLIVDCAHGFGLIPCANSDIYIGTLSKAVGVLGGYVCAS
HCCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHH
EVVIKYIQNKAKTFIYTTALPPMVIAAANAALDIISASVNDIPIKLAKFFCKNLNLAEPS
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
SHIVPLIMKNMNSVLHAQQVLREAGFLVVAIRPPTVPTPRLRFVFSVNHSLSDIEKLCEV
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHH
IKRNKLA
HHHCCCC
>Mature Secondary Structure
MLDEILNQELIKLKVDNLYREPSTVTRSEVGCLVCQNNERLVSFSCNDYLGLIGHPLLKE
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCEEEEEECCCHHHHHCCHHHHH
SAINAINNYGVGAGASRMVTGNNILYQCLEDKLAKLYHTEMALVFSSGYLTNVGVISALV
HHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHH
HRHDMIISDKLVHSSIIDGIKLSSAKHYRFEHNDYGHCEDILKKYRRLHKYCFIVVEQVY
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SMNGDIAPIDQLKKLAEKYSAWLIVDCAHGFGLIPCANSDIYIGTLSKAVGVLGGYVCAS
HCCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHH
EVVIKYIQNKAKTFIYTTALPPMVIAAANAALDIISASVNDIPIKLAKFFCKNLNLAEPS
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
SHIVPLIMKNMNSVLHAQQVLREAGFLVVAIRPPTVPTPRLRFVFSVNHSLSDIEKLCEV
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHH
IKRNKLA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA