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The map label for this gene is hemB [H]

Identifier: 88658240

GI number: 88658240

Start: 835748

End: 836743

Strand: Direct

Name: hemB [H]

Synonym: ECH_0821

Alternate gene names: 88658240

Gene position: 835748-836743 (Clockwise)

Preceding gene: 88657576

Following gene: 88658554

Centisome position: 71.05

GC content: 33.33

Gene sequence:

>996_bases
ATGCAATTTCCTAATACTAGATTAAGACGTAAACGTTCAAATGAGTGGTTACGTAATTTAGTAAGAGAAACTAGTTTATC
TATACACGACCTAATATTACCACTGTTTGTACATGATCGTGATGGGGTATCAGAACCTGTAAAACAATTACCTGACATCA
AAAGATACTCTATCCCTGAATTATTAAAAGTGGTAGAACATGCTGCAAATTTAGGCATAAATGCTATATCGTTGTTTCCA
GTAGTAGAAACCCATTTGAAATCTGAAAATGCAGAAGAAGCATTTAACCCTAATAATCTAATATGCAGAGCAATAAAAGA
AGTCAAAAAACACATACCAACTATTGGCATAATAGCCGATGTTGCATTAGATCCTTATACAATTTCAGGACATGATGGAA
TTGTAATCAACAAGCAAATTGATAATGACGCAACCTTAGAAGTTTTATATAAGCAAGCTTTAGTCTTAGCAGCAGCAGGT
TGTGACATTGTAGCACCATCAGATATGATGGATGGAAGAGTCAAAAAAATCAGAAGTGCTTTAGATAACTGTAATTTCCA
AGATGTATGCATACTATCTTACTCTGTAAAATACTGTTCCAGTTTTTATAAACCATTTCGTGAAGCTGTAGGATCTCGCT
CAATATCCCAAACAATAGATAAAAGTACATACCAATTAGATATTGCAAACATAAATGAAGCAATACTTGAGGCACAAATG
GATATCGATGAAAGTGCAGATATTATTATGATAAAACCAGGGATGCCATATCTAGATGTAATTAAGTCCATTAGTAGCAG
ATGTCAGATACCAGTATTTGCATACCAAGTAAGTGGAGAATATGCAATGATTAAAGCTGCATCACAAAATCACTTGCTAG
ATTATGATTCTGTTATCTACGAATCTCTATTAGGATTTAAGCGTGCAGGTGCAAAAAGTATAATTACTTACGCTGCAATA
GATGCAGCAAATATACTCATAAAAAACAAAATGTAA

Upstream 100 bases:

>100_bases
CACTATAAAAGCTCACAAATTATCTAATTAAAAAATAAGTTGATACTTATCAATCAAATGATTAAATTAGTACACACATG
TAACACAATAAACTAACAAT

Downstream 100 bases:

>100_bases
GAAATCTACAACATACTATTTAATACATCAAAACACAAAAGTTAGGTGTTCATACTTGTAATTCTCTAAATATTATAGAA
TGGAAATGTATCATAACTTA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQFPNTRLRRKRSNEWLRNLVRETSLSIHDLILPLFVHDRDGVSEPVKQLPDIKRYSIPELLKVVEHAANLGINAISLFP
VVETHLKSENAEEAFNPNNLICRAIKEVKKHIPTIGIIADVALDPYTISGHDGIVINKQIDNDATLEVLYKQALVLAAAG
CDIVAPSDMMDGRVKKIRSALDNCNFQDVCILSYSVKYCSSFYKPFREAVGSRSISQTIDKSTYQLDIANINEAILEAQM
DIDESADIIMIKPGMPYLDVIKSISSRCQIPVFAYQVSGEYAMIKAASQNHLLDYDSVIYESLLGFKRAGAKSIITYAAI
DAANILIKNKM

Sequences:

>Translated_331_residues
MQFPNTRLRRKRSNEWLRNLVRETSLSIHDLILPLFVHDRDGVSEPVKQLPDIKRYSIPELLKVVEHAANLGINAISLFP
VVETHLKSENAEEAFNPNNLICRAIKEVKKHIPTIGIIADVALDPYTISGHDGIVINKQIDNDATLEVLYKQALVLAAAG
CDIVAPSDMMDGRVKKIRSALDNCNFQDVCILSYSVKYCSSFYKPFREAVGSRSISQTIDKSTYQLDIANINEAILEAQM
DIDESADIIMIKPGMPYLDVIKSISSRCQIPVFAYQVSGEYAMIKAASQNHLLDYDSVIYESLLGFKRAGAKSIITYAAI
DAANILIKNKM
>Mature_331_residues
MQFPNTRLRRKRSNEWLRNLVRETSLSIHDLILPLFVHDRDGVSEPVKQLPDIKRYSIPELLKVVEHAANLGINAISLFP
VVETHLKSENAEEAFNPNNLICRAIKEVKKHIPTIGIIADVALDPYTISGHDGIVINKQIDNDATLEVLYKQALVLAAAG
CDIVAPSDMMDGRVKKIRSALDNCNFQDVCILSYSVKYCSSFYKPFREAVGSRSISQTIDKSTYQLDIANINEAILEAQM
DIDESADIIMIKPGMPYLDVIKSISSRCQIPVFAYQVSGEYAMIKAASQNHLLDYDSVIYESLLGFKRAGAKSIITYAAI
DAANILIKNKM

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=297, Percent_Identity=35.6902356902357, Blast_Score=187, Evalue=8e-48,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=41.8238993710692, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321398, Length=307, Percent_Identity=35.1791530944625, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21358291, Length=314, Percent_Identity=35.9872611464968, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36947; Mature: 36947

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFPNTRLRRKRSNEWLRNLVRETSLSIHDLILPLFVHDRDGVSEPVKQLPDIKRYSIPE
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCHHHHHHHCCCCCCCCHHH
LLKVVEHAANLGINAISLFPVVETHLKSENAEEAFNPNNLICRAIKEVKKHIPTIGIIAD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCEEEEHH
VALDPYTISGHDGIVINKQIDNDATLEVLYKQALVLAAAGCDIVAPSDMMDGRVKKIRSA
HCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LDNCNFQDVCILSYSVKYCSSFYKPFREAVGSRSISQTIDKSTYQLDIANINEAILEAQM
HHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHHHC
DIDESADIIMIKPGMPYLDVIKSISSRCQIPVFAYQVSGEYAMIKAASQNHLLDYDSVIY
CCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHH
ESLLGFKRAGAKSIITYAAIDAANILIKNKM
HHHHHHHHCCHHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure
MQFPNTRLRRKRSNEWLRNLVRETSLSIHDLILPLFVHDRDGVSEPVKQLPDIKRYSIPE
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCHHHHHHHCCCCCCCCHHH
LLKVVEHAANLGINAISLFPVVETHLKSENAEEAFNPNNLICRAIKEVKKHIPTIGIIAD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCEEEEHH
VALDPYTISGHDGIVINKQIDNDATLEVLYKQALVLAAAGCDIVAPSDMMDGRVKKIRSA
HCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LDNCNFQDVCILSYSVKYCSSFYKPFREAVGSRSISQTIDKSTYQLDIANINEAILEAQM
HHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHHHC
DIDESADIIMIKPGMPYLDVIKSISSRCQIPVFAYQVSGEYAMIKAASQNHLLDYDSVIY
CCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHH
ESLLGFKRAGAKSIITYAAIDAANILIKNKM
HHHHHHHHCCHHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]