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The map label for this gene is nadE [H]

Identifier: 88658554

GI number: 88658554

Start: 836886

End: 838403

Strand: Direct

Name: nadE [H]

Synonym: ECH_0822

Alternate gene names: 88658554

Gene position: 836886-838403 (Clockwise)

Preceding gene: 88658240

Following gene: 88658118

Centisome position: 71.15

GC content: 28.39

Gene sequence:

>1518_bases
ATGTACAATTCATCAATCTTTATTTCCCAATTAAATTACAATCCTGAAAATATTGATTACAACTATGATAGCATTACAAA
ATCATATCAAGAGTCAGTATATCAAGCAGCAGATATAGCCATATTTTCAAGATACGCAGTATCAGGACACATACAAAAAA
TTCCAACTTTACATAAAAATTTTTTCAATCAATGTACTAATATAATAAATAAACTAGCACTACAAACTAATAATAAAAAC
ACAGCAATAATCATTGGTAGCATAAAAAAGAAAAACAATGTCATATTTGAAACAATTTATCTGATAAAAAATGGAGAAAT
TACAGAACTAATAGAATTCCCTACAGATTCTCAATCTTCTGATATCTCAGCAACGTTTACAATTAATAACATCAATGTAT
TGCTACTTCCTATCTCAAAAATACCTAAATTACCTATTCATAATAATGACACGTTATTACTGATTATGGATAATACACAA
TATACAAAAAATACAAATTCTAGAGATGATTTAATTTCAAAGTTAACATTCAATAAATACGCAGTCTATATTAACCAAAT
AGGAGGATACAACAATTTAGTTTTTCATGGAAAATCCTTTTTTACTTTCAATGGTCAATACAATTTCTTAAACATGTGGC
AAGAAGATAGTAAAGTTTGCAAAGCAGATTTAGCAAAACAGGACAACCATACCATAACATCCAACAGCATTGAATCAGAT
TACCAAGCACTTATGTTAGCATTAAGAGACTATATATATAAAAATGCTATGACCAGTGTAATCATGGGATTATCTGGAGG
TATAGACTCTGCACTTGTAGCTTCAATTGCAGCAGATGCGCTTGGCCCTAATAACGTACATTCACTCATGTTACCAACAA
AATATACATCTCAAATGAGTATTACTGATTCTAAAGAATGCGCTATGAGATTAGGTATTTCTTACAATATATTATCGATA
GAAGAAATATTCCAAACTTCACTAGCAGCATTACAAAATACTTTCATAGACACTCAAGAAGATATTACTGAAGAAAATTT
ACAAGCTAGAATCAGAGGTAATATATTAATGGCATTAAGCAATAAGTTTAAATTTATGTTATTATCAACAGGTAATAAAT
CAGAATTATTTGTTGGTTATGCAACATTATATGGAGATATGTGTGGAGGATTTGCTCCAATAAAAGATATATATAAAACT
AGAGTATACGAACTCAGTAAATGGAGGAATAAAAATATACCAGTAAATAGTTTATGTAAAAAAATTGACGTTATTCCTTC
TCAGATTATAGAAAAACATCCATCTGCTGAACTACGTTACAACCAAAAAGATCAAGATACTCTACCTGATTATGCAATAT
TAGATTCAATACTTACATTACTTATCGATCAAAACCATTCTACAGAAGATATAATAAAAAAAGGATATAATATTCAAGAT
GTTAATTATATTATACACTTAATACAAAAATCTCAATTTAAAAGGGAGCAAGCCTCTACTGGACCACTCATCAATTAA

Upstream 100 bases:

>100_bases
TAGGTGTTCATACTTGTAATTCTCTAAATATTATAGAATGGAAATGTATCATAACTTATTATAGAATTAATGCGGTAAGT
ATAGAATAAGTCAGATAAGT

Downstream 100 bases:

>100_bases
TTTAGGCAGAAAACAGTGAAGGTTATAAAATCATTCAATATCCCTTATTTAATATTAAACTTATATCTGCAATATTAAAA
ACACCTACCGTTACAAAAAA

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MYNSSIFISQLNYNPENIDYNYDSITKSYQESVYQAADIAIFSRYAVSGHIQKIPTLHKNFFNQCTNIINKLALQTNNKN
TAIIIGSIKKKNNVIFETIYLIKNGEITELIEFPTDSQSSDISATFTINNINVLLLPISKIPKLPIHNNDTLLLIMDNTQ
YTKNTNSRDDLISKLTFNKYAVYINQIGGYNNLVFHGKSFFTFNGQYNFLNMWQEDSKVCKADLAKQDNHTITSNSIESD
YQALMLALRDYIYKNAMTSVIMGLSGGIDSALVASIAADALGPNNVHSLMLPTKYTSQMSITDSKECAMRLGISYNILSI
EEIFQTSLAALQNTFIDTQEDITEENLQARIRGNILMALSNKFKFMLLSTGNKSELFVGYATLYGDMCGGFAPIKDIYKT
RVYELSKWRNKNIPVNSLCKKIDVIPSQIIEKHPSAELRYNQKDQDTLPDYAILDSILTLLIDQNHSTEDIIKKGYNIQD
VNYIIHLIQKSQFKREQASTGPLIN

Sequences:

>Translated_505_residues
MYNSSIFISQLNYNPENIDYNYDSITKSYQESVYQAADIAIFSRYAVSGHIQKIPTLHKNFFNQCTNIINKLALQTNNKN
TAIIIGSIKKKNNVIFETIYLIKNGEITELIEFPTDSQSSDISATFTINNINVLLLPISKIPKLPIHNNDTLLLIMDNTQ
YTKNTNSRDDLISKLTFNKYAVYINQIGGYNNLVFHGKSFFTFNGQYNFLNMWQEDSKVCKADLAKQDNHTITSNSIESD
YQALMLALRDYIYKNAMTSVIMGLSGGIDSALVASIAADALGPNNVHSLMLPTKYTSQMSITDSKECAMRLGISYNILSI
EEIFQTSLAALQNTFIDTQEDITEENLQARIRGNILMALSNKFKFMLLSTGNKSELFVGYATLYGDMCGGFAPIKDIYKT
RVYELSKWRNKNIPVNSLCKKIDVIPSQIIEKHPSAELRYNQKDQDTLPDYAILDSILTLLIDQNHSTEDIIKKGYNIQD
VNYIIHLIQKSQFKREQASTGPLIN
>Mature_505_residues
MYNSSIFISQLNYNPENIDYNYDSITKSYQESVYQAADIAIFSRYAVSGHIQKIPTLHKNFFNQCTNIINKLALQTNNKN
TAIIIGSIKKKNNVIFETIYLIKNGEITELIEFPTDSQSSDISATFTINNINVLLLPISKIPKLPIHNNDTLLLIMDNTQ
YTKNTNSRDDLISKLTFNKYAVYINQIGGYNNLVFHGKSFFTFNGQYNFLNMWQEDSKVCKADLAKQDNHTITSNSIESD
YQALMLALRDYIYKNAMTSVIMGLSGGIDSALVASIAADALGPNNVHSLMLPTKYTSQMSITDSKECAMRLGISYNILSI
EEIFQTSLAALQNTFIDTQEDITEENLQARIRGNILMALSNKFKFMLLSTGNKSELFVGYATLYGDMCGGFAPIKDIYKT
RVYELSKWRNKNIPVNSLCKKIDVIPSQIIEKHPSAELRYNQKDQDTLPDYAILDSILTLLIDQNHSTEDIIKKGYNIQD
VNYIIHLIQKSQFKREQASTGPLIN

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 57200; Mature: 57200

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNSSIFISQLNYNPENIDYNYDSITKSYQESVYQAADIAIFSRYAVSGHIQKIPTLHKN
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FFNQCTNIINKLALQTNNKNTAIIIGSIKKKNNVIFETIYLIKNGEITELIEFPTDSQSS
HHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCEEEEEEEEEECCCEEEEEECCCCCCCC
DISATFTINNINVLLLPISKIPKLPIHNNDTLLLIMDNTQYTKNTNSRDDLISKLTFNKY
CEEEEEEEECCEEEEEEHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCE
AVYINQIGGYNNLVFHGKSFFTFNGQYNFLNMWQEDSKVCKADLAKQDNHTITSNSIESD
EEEEECCCCCCCEEEECCEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCEECCCCCHHH
YQALMLALRDYIYKNAMTSVIMGLSGGIDSALVASIAADALGPNNVHSLMLPTKYTSQMS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEE
ITDSKECAMRLGISYNILSIEEIFQTSLAALQNTFIDTQEDITEENLQARIRGNILMALS
CCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
NKFKFMLLSTGNKSELFVGYATLYGDMCGGFAPIKDIYKTRVYELSKWRNKNIPVNSLCK
CCEEEEEEECCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
KIDVIPSQIIEKHPSAELRYNQKDQDTLPDYAILDSILTLLIDQNHSTEDIIKKGYNIQD
HHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCH
VNYIIHLIQKSQFKREQASTGPLIN
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYNSSIFISQLNYNPENIDYNYDSITKSYQESVYQAADIAIFSRYAVSGHIQKIPTLHKN
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FFNQCTNIINKLALQTNNKNTAIIIGSIKKKNNVIFETIYLIKNGEITELIEFPTDSQSS
HHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCEEEEEEEEEECCCEEEEEECCCCCCCC
DISATFTINNINVLLLPISKIPKLPIHNNDTLLLIMDNTQYTKNTNSRDDLISKLTFNKY
CEEEEEEEECCEEEEEEHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCE
AVYINQIGGYNNLVFHGKSFFTFNGQYNFLNMWQEDSKVCKADLAKQDNHTITSNSIESD
EEEEECCCCCCCEEEECCEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCEECCCCCHHH
YQALMLALRDYIYKNAMTSVIMGLSGGIDSALVASIAADALGPNNVHSLMLPTKYTSQMS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEE
ITDSKECAMRLGISYNILSIEEIFQTSLAALQNTFIDTQEDITEENLQARIRGNILMALS
CCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
NKFKFMLLSTGNKSELFVGYATLYGDMCGGFAPIKDIYKTRVYELSKWRNKNIPVNSLCK
CCEEEEEEECCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
KIDVIPSQIIEKHPSAELRYNQKDQDTLPDYAILDSILTLLIDQNHSTEDIIKKGYNIQD
HHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCH
VNYIIHLIQKSQFKREQASTGPLIN
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]