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The map label for this gene is dnaN [H]

Identifier: 88658239

GI number: 88658239

Start: 1036569

End: 1037117

Strand: Direct

Name: dnaN [H]

Synonym: ECH_1010

Alternate gene names: 88658239

Gene position: 1036569-1037117 (Clockwise)

Preceding gene: 88658288

Following gene: 88657628

Centisome position: 88.13

GC content: 27.69

Gene sequence:

>549_bases
GTGTCTGCTCAACTTCTTTATGATATTGTAAAAAAGTTGCCTAATGATATTGAAATCAAATTTTTTATTGGTGAAGATGG
AAGATTAACTATAAAATGTGGTCGTACGGAGTTTTTTCTTCCTGTAGTTGATATAGAGAAGTTTCCTATTATTGAAAATA
ATGAATTACAAAACAAATTTACTTTGCAAGTCTCTGAATTGGTTAGTTTGTTTGGTAAAACTAAATTTGCAATTTCCATA
GAAGAGAGTAGATTTAATTTGAATGGATTATATGTTCATACGTCCAATAATATCTTATGTTGTGCTGCTACAGATGGACA
TAGGTTGTCGTTAACTAAGTTGAGTCAATCTCAGAATGTATTGTCAAATTTTGGTATTATAGTTCCCAAAAAAACAGTTA
ATGAAATTCTGAAGATAGTAAGTCATGGTAGTGGTAATGATTTAATATCAATTAGTTTATCAGATAGGAAGATTTGTTTT
GAATATAAAGAATATATTTTAATATCTAAGGTAATAGATAGTACTTTTGCGTCAGATTACATATTATAA

Upstream 100 bases:

>100_bases
ACTCTCAACTCTTTTAATCGCTAAGTTAACATCTACAGATCTTGATATTTCAATAATTGCATCTATTACTGCCCATGTAA
ACGTTAATGGAGTTGTAGTA

Downstream 100 bases:

>100_bases
ATATGACTAAACGACATTAACACTACTACGATAATTGACCAATCTGTTAAACAAAAACTAAACCTACTACCCTCTATATA
CTACAATACACTCAATGTTA

Product: DNA polymerase III, beta subunit, truncation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MSAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVSLFGKTKFAISI
EESRFNLNGLYVHTSNNILCCAATDGHRLSLTKLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICF
EYKEYILISKVIDSTFASDYIL

Sequences:

>Translated_182_residues
MSAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVSLFGKTKFAISI
EESRFNLNGLYVHTSNNILCCAATDGHRLSLTKLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICF
EYKEYILISKVIDSTFASDYIL
>Mature_181_residues
SAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVSLFGKTKFAISIE
ESRFNLNGLYVHTSNNILCCAATDGHRLSLTKLSQSQNVLSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFE
YKEYILISKVIDSTFASDYIL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=180, Percent_Identity=28.3333333333333, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 20545; Mature: 20414

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKF
CCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCEEEEEEEECCCCCCCEECCCCCCEE
TLQVSELVSLFGKTKFAISIEESRFNLNGLYVHTSNNILCCAATDGHRLSLTKLSQSQNV
EEEHHHHHHHHCCEEEEEEEECCEECEEEEEEEECCCEEEEEECCCCEEEEEECCCHHHH
LSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVIDSTFASDY
HHHCCEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCC
IL
CC
>Mature Secondary Structure 
SAQLLYDIVKKLPNDIEIKFFIGEDGRLTIKCGRTEFFLPVVDIEKFPIIENNELQNKF
CHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCEEEEEEEECCCCCCCEECCCCCCEE
TLQVSELVSLFGKTKFAISIEESRFNLNGLYVHTSNNILCCAATDGHRLSLTKLSQSQNV
EEEHHHHHHHHCCEEEEEEEECCEECEEEEEEEECCCEEEEEECCCCEEEEEECCCHHHH
LSNFGIIVPKKTVNEILKIVSHGSGNDLISISLSDRKICFEYKEYILISKVIDSTFASDY
HHHCCEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCC
IL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]