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The map label for this gene is cyoE

Identifier: 88658064

GI number: 88658064

Start: 1030528

End: 1031415

Strand: Direct

Name: cyoE

Synonym: ECH_1004

Alternate gene names: 88658064

Gene position: 1030528-1031415 (Clockwise)

Preceding gene: 88658568

Following gene: 88657834

Centisome position: 87.61

GC content: 32.66

Gene sequence:

>888_bases
ATGAGTTCTAAATGTGAAAGTGTTAAACCACAGCTTGTAAATGAAGTATTAGGCTATTGGGATCTACTCAAACCAAAAAT
CATGTATTTAGTAGTTCTTACAGGAATTACAGGAATGATTATTGCTCCAGGAAATATTCATCCTTTTATCGGTATCATCA
GTACCTTATGCATAGCACTAGGATCTGGTGCTGCAGGAGCAATTAATATGTGGTATGACAGTGATATAGACGCATTAATG
CAACGTACCAAAAATCGTCCTATACCTTCTGGAAAAATTGCTAGATCTACTGCAATTGAATTGGGATTAGTGTTATCCGT
TATTTCTGTAACAGTAATGATGATCGCCGTTAACTATCTATCAGGAATATTACTAGCTATCAGTATAGGATTCTACTCAC
TTGTATACACAATGTATCTTAAAAGAAGAACACCACAAAATATAGTCATAGGAGGAATTGCAGGAGCACTGCCTCCAATT
ATTGGATGGACTTCTGTAACTAACGCCATCTCCATAGAAAGCTTGATATTATTTCTAATTATATTTGTATGGACTCCTCC
ACACTTTTGGGCATTATCTTTATTAAACTATCAGGAATACGAAAAGGCTAAAGTACCAATGTTACCTGTCACACATGGAA
TATTCACTACCAAAATTTATATACTAGTATATAGTATCATACTATTTATTATAACACTATTACCTGGAATATTTTTAAAA
GATTGTTTGTTATATGAAACTTGTGCAATACCACTTGGCATGACATTCGTGTTCCATGCTTTCAAGGTATTTGTATCAAT
CAACCACTATAAATACAAAGCAATGTTTACTTATTCTATTGCTTACCTCTTTATTTTATTCATATGTATTATTATCTCAA
GTTTTTAA

Upstream 100 bases:

>100_bases
AATGTCCTAGCAATCCATGGGGAGGAGATACATTAGAATGGACAATACCATCACCAGCACCTTTCCATACCTTTGAAGAA
ATACCAAAGGTTGATTAAGC

Downstream 100 bases:

>100_bases
CATCCCTTTTAATTAAGAGATTCCAAAACATCAATAAAGCCTCACTACAATTATAAACTATTCTTTGTTATTTAACCAAA
GGTTCTTTATACCATCCAGT

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSSKCESVKPQLVNEVLGYWDLLKPKIMYLVVLTGITGMIIAPGNIHPFIGIISTLCIALGSGAAGAINMWYDSDIDALM
QRTKNRPIPSGKIARSTAIELGLVLSVISVTVMMIAVNYLSGILLAISIGFYSLVYTMYLKRRTPQNIVIGGIAGALPPI
IGWTSVTNAISIESLILFLIIFVWTPPHFWALSLLNYQEYEKAKVPMLPVTHGIFTTKIYILVYSIILFIITLLPGIFLK
DCLLYETCAIPLGMTFVFHAFKVFVSINHYKYKAMFTYSIAYLFILFICIIISSF

Sequences:

>Translated_295_residues
MSSKCESVKPQLVNEVLGYWDLLKPKIMYLVVLTGITGMIIAPGNIHPFIGIISTLCIALGSGAAGAINMWYDSDIDALM
QRTKNRPIPSGKIARSTAIELGLVLSVISVTVMMIAVNYLSGILLAISIGFYSLVYTMYLKRRTPQNIVIGGIAGALPPI
IGWTSVTNAISIESLILFLIIFVWTPPHFWALSLLNYQEYEKAKVPMLPVTHGIFTTKIYILVYSIILFIITLLPGIFLK
DCLLYETCAIPLGMTFVFHAFKVFVSINHYKYKAMFTYSIAYLFILFICIIISSF
>Mature_294_residues
SSKCESVKPQLVNEVLGYWDLLKPKIMYLVVLTGITGMIIAPGNIHPFIGIISTLCIALGSGAAGAINMWYDSDIDALMQ
RTKNRPIPSGKIARSTAIELGLVLSVISVTVMMIAVNYLSGILLAISIGFYSLVYTMYLKRRTPQNIVIGGIAGALPPII
GWTSVTNAISIESLILFLIIFVWTPPHFWALSLLNYQEYEKAKVPMLPVTHGIFTTKIYILVYSIILFIITLLPGIFLKD
CLLYETCAIPLGMTFVFHAFKVFVSINHYKYKAMFTYSIAYLFILFICIIISSF

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=255, Percent_Identity=28.6274509803922, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786631, Length=217, Percent_Identity=29.9539170506912, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71998235, Length=288, Percent_Identity=24.6527777777778, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325085, Length=218, Percent_Identity=30.2752293577982, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19921064, Length=275, Percent_Identity=27.2727272727273, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COXX_EHRCR (Q2GFJ2)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507791.1
- STRING:   Q2GFJ2
- GeneID:   3927251
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_1004
- TIGR:   ECH_1004
- eggNOG:   COG0109
- HOGENOM:   HBG656003
- OMA:   LLKPRIM
- PhylomeDB:   Q2GFJ2
- ProtClustDB:   PRK04375
- BioCyc:   ECHA205920:ECH_1004-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.- [C]

Molecular weight: Translated: 32816; Mature: 32685

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1db5c198)-; HASH(0x1d3a1ee4)-; HASH(0x1e02664c)-; HASH(0x1df5e1a4)-; HASH(0x1e02dbc4)-; HASH(0x1e462aa8)-; HASH(0x1d03b41c)-; HASH(0x1e0345ec)-; HASH(0x1dec4fe4)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKCESVKPQLVNEVLGYWDLLKPKIMYLVVLTGITGMIIAPGNIHPFIGIISTLCIAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
GSGAAGAINMWYDSDIDALMQRTKNRPIPSGKIARSTAIELGLVLSVISVTVMMIAVNYL
CCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGILLAISIGFYSLVYTMYLKRRTPQNIVIGGIAGALPPIIGWTSVTNAISIESLILFLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFVWTPPHFWALSLLNYQEYEKAKVPMLPVTHGIFTTKIYILVYSIILFIITLLPGIFLK
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DCLLYETCAIPLGMTFVFHAFKVFVSINHYKYKAMFTYSIAYLFILFICIIISSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SSKCESVKPQLVNEVLGYWDLLKPKIMYLVVLTGITGMIIAPGNIHPFIGIISTLCIAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
GSGAAGAINMWYDSDIDALMQRTKNRPIPSGKIARSTAIELGLVLSVISVTVMMIAVNYL
CCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGILLAISIGFYSLVYTMYLKRRTPQNIVIGGIAGALPPIIGWTSVTNAISIESLILFLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFVWTPPHFWALSLLNYQEYEKAKVPMLPVTHGIFTTKIYILVYSIILFIITLLPGIFLK
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DCLLYETCAIPLGMTFVFHAFKVFVSINHYKYKAMFTYSIAYLFILFICIIISSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA