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The map label for this gene is ubiE [H]

Identifier: 88657835

GI number: 88657835

Start: 1022880

End: 1023572

Strand: Direct

Name: ubiE [H]

Synonym: ECH_0998

Alternate gene names: 88657835

Gene position: 1022880-1023572 (Clockwise)

Preceding gene: 88657724

Following gene: 88658567

Centisome position: 86.96

GC content: 31.17

Gene sequence:

>693_bases
ATGCATGAAAGATCAAAGTTTGTTAATCAAGTTTTTACTTCAGTAGCCTCACGCTACGATTTAATGAATGACATAATGAG
CTTTGGTCTTCACAGGCTATGGAAAGATAAAGTGATTCAGAATATTACTAAGGGTAGCTTACTTGATGTTGCATGCGGTA
CTGGAGACATAGCTATAAAAGTAGCAAAAAATACACAAGATGTAGACATAACAGTATGTGATATTAATCCAAAAATGCTA
AATTATGGACGAGATAATGCTGTTAATGCGAATGTATTAAATCTTAAATGGGTATGTAGTAATGCAGAGCAGCTTCCATT
TCTAGATGACAGTTTTGACTATTATACAATTTCTTTCGGTATACGTAATGTTTTACATAGACAGCTTGCATTAAATGAAG
CATATAGAGTCTTAAAACAAGGAGGAAAATTCATATGCTTAGAATTCTCTCCATTAAAAGAAAGTCACCCTCTTTACAAA
CTGTATAACTTTTATTCATTTAATATTATCCCTCACGTAGGACAAGTAGTAGCAAAGGATAGAGCTTCATACCAATACTT
AGTAGACAGTATAAGTGAATTCCCTTACCAAGAAGATTTTGCTCAAGAAATCAAAAATTCTAATTTTGTTCAAATAAAAT
ATAATAATTTATGTTTTGGCATAGTTACGTTATATACTGCTTGGAAAATATAA

Upstream 100 bases:

>100_bases
AAAATAGTGGTAGGACATATGTGATAGATAGCGAATATGTAAAGAAAAAGCTAGAAGACATTGCAAAACAATTAGATTTA
TCAAAATTTATATTATAGTT

Downstream 100 bases:

>100_bases
CTAACCTTATATATAAAAGTTCTTCCCTTACAGAATAATACCATCAAGAATAATTTATATAAGAACTTCCCACACTATAT
CACTTATCAACATAAATCTA

Product: ubiquinone/menaquinone biosynthesis methlytransferase UbiE

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MHERSKFVNQVFTSVASRYDLMNDIMSFGLHRLWKDKVIQNITKGSLLDVACGTGDIAIKVAKNTQDVDITVCDINPKML
NYGRDNAVNANVLNLKWVCSNAEQLPFLDDSFDYYTISFGIRNVLHRQLALNEAYRVLKQGGKFICLEFSPLKESHPLYK
LYNFYSFNIIPHVGQVVAKDRASYQYLVDSISEFPYQEDFAQEIKNSNFVQIKYNNLCFGIVTLYTAWKI

Sequences:

>Translated_230_residues
MHERSKFVNQVFTSVASRYDLMNDIMSFGLHRLWKDKVIQNITKGSLLDVACGTGDIAIKVAKNTQDVDITVCDINPKML
NYGRDNAVNANVLNLKWVCSNAEQLPFLDDSFDYYTISFGIRNVLHRQLALNEAYRVLKQGGKFICLEFSPLKESHPLYK
LYNFYSFNIIPHVGQVVAKDRASYQYLVDSISEFPYQEDFAQEIKNSNFVQIKYNNLCFGIVTLYTAWKI
>Mature_230_residues
MHERSKFVNQVFTSVASRYDLMNDIMSFGLHRLWKDKVIQNITKGSLLDVACGTGDIAIKVAKNTQDVDITVCDINPKML
NYGRDNAVNANVLNLKWVCSNAEQLPFLDDSFDYYTISFGIRNVLHRQLALNEAYRVLKQGGKFICLEFSPLKESHPLYK
LYNFYSFNIIPHVGQVVAKDRASYQYLVDSISEFPYQEDFAQEIKNSNFVQIKYNNLCFGIVTLYTAWKI

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=268, Percent_Identity=43.2835820895522, Blast_Score=211, Evalue=4e-55,
Organism=Escherichia coli, GI48994983, Length=233, Percent_Identity=43.7768240343348, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17557039, Length=233, Percent_Identity=44.206008583691, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323526, Length=247, Percent_Identity=41.7004048582996, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24641702, Length=246, Percent_Identity=43.9024390243902, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26498; Mature: 26498

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERSKFVNQVFTSVASRYDLMNDIMSFGLHRLWKDKVIQNITKGSLLDVACGTGDIAIK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEE
VAKNTQDVDITVCDINPKMLNYGRDNAVNANVLNLKWVCSNAEQLPFLDDSFDYYTISFG
EECCCCCEEEEEEECCHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEHHH
IRNVLHRQLALNEAYRVLKQGGKFICLEFSPLKESHPLYKLYNFYSFNIIPHVGQVVAKD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHEEECCHHHHHHHHHH
RASYQYLVDSISEFPYQEDFAQEIKNSNFVQIKYNNLCFGIVTLYTAWKI
HHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MHERSKFVNQVFTSVASRYDLMNDIMSFGLHRLWKDKVIQNITKGSLLDVACGTGDIAIK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEE
VAKNTQDVDITVCDINPKMLNYGRDNAVNANVLNLKWVCSNAEQLPFLDDSFDYYTISFG
EECCCCCEEEEEEECCHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEHHH
IRNVLHRQLALNEAYRVLKQGGKFICLEFSPLKESHPLYKLYNFYSFNIIPHVGQVVAKD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHEEECCHHHHHHHHHH
RASYQYLVDSISEFPYQEDFAQEIKNSNFVQIKYNNLCFGIVTLYTAWKI
HHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA