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The map label for this gene is hslU [H]

Identifier: 88657724

GI number: 88657724

Start: 1021414

End: 1022877

Strand: Direct

Name: hslU [H]

Synonym: ECH_0997

Alternate gene names: 88657724

Gene position: 1021414-1022877 (Clockwise)

Preceding gene: 88658351

Following gene: 88657835

Centisome position: 86.84

GC content: 33.06

Gene sequence:

>1464_bases
ATGTTTGTTACACCAAATAATAAACTCAAATTGAATAATGATACAACAAATAATATAAATGATGAACAAGCTAGTAGTGA
AGTACTAAATAGTGAAAACGCTATTAGCACAGAAGGATATGAAGATGACATAAATCTAGATGACTTATACAACCCACAAG
AATTAACTCCTCAGCAAATTACACAGGAACTAGATAGGTTTATTATAGGACAAGCAGACGCAAAACGTGCTGTAGCCATT
GCCTTAAGAAATCGCTGGCGTCGTAACAGGGTACCTGAACCATTAAGAGAAGAGATTATCCCTAAAAACATCCTAATGAT
AGGACATACAGGTATTGGTAAAACAGAAATAGCTCGCAGGTTAGCAAAACTTGCAAAAGCACCTTTCATAAAAGTTGAAG
CTACAAAATTTACTGAAATAGGATATGTAGGGAGAGATGTAGACTCTATTATACGTGACTTAGTTGATGTAGCAATCAAT
CTTGAAAAAGAAAAAAGTCGTAAATTTGTAGAGACAAAAGCAAAATCTTTAGCAGAAAATATAATTCTTGAAGCACTGGT
AGGAGCTGATGCAAGTCAAGAGACAAAAACTATTTTTCAAGAAAAGCTAAGAAATGGTGAATTTGAAAATTTTGAGATCT
CCATATCCATAAAGGAAAGTAAAAATGCAATCCCTTCTATTGATATTCCAAATATTCCAGGAAATCAAGTTGGCATTATG
AATATCAATGAGATTGTACATAAAATGCTAGGAAATAACAAACAACTTAAGACTATAAAAGTTACTGTAAAAGAGGCAAG
AGAACTGCTAATTAATGAAGAAAGCGAAAAATTAATGGATGAAGATAAAATCATCAAAGACGCTCTTTTGTTAGCAAGTA
ACGACGGCATCGTATTTCTAGATGAAATAGACAAAATTGCAGCTCGTACAGAAATCAGAGGAGAAGTGAACAGAGAAGGT
GTACAAAGAGATCTTTTACCATTACTTGAAGGAACAAGTGTAACAACAAAGTATGGTACTATTACAACCGATCACATTTT
ATTCATAGCATCTGGAGCATTTCACTTGGCTAAACCTTCTGACTTATTACCTGAGTTACAAGGACGTCTTCCTATACGAG
TAGAACTGAAACCGCTTAGTAAAGATGATTTAGTACGAATTTTAACCGAACCAGAATCAAGCTTATTAAAGCAATACTGC
GCATTAATGAAAACAGAAAATATTACTATTGACTTTACTGATGAGGGAGTATCTACTATAGCTGAAATAGCATCTACAGT
TAACAGAGAGGTAGAAAATATTGGAGCTCGTAGATTACATACCATTTTAGAAAAGCTAATGGAAGATATCAGTTATACTG
CAACAGAAAATAGTGGTAGGACATATGTGATAGATAGCGAATATGTAAAGAAAAAGCTAGAAGACATTGCAAAACAATTA
GATTTATCAAAATTTATATTATAG

Upstream 100 bases:

>100_bases
ATGACTTTGGAATATATAATTACCACTGCAATGAAAATAGCTTCTGAAATATGTATTTATACAAACAACAATATTATTAT
GGAAAAAATAGAGGATTAAA

Downstream 100 bases:

>100_bases
TTATGCATGAAAGATCAAAGTTTGTTAATCAAGTTTTTACTTCAGTAGCCTCACGCTACGATTTAATGAATGACATAATG
AGCTTTGGTCTTCACAGGCT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MFVTPNNKLKLNNDTTNNINDEQASSEVLNSENAISTEGYEDDINLDDLYNPQELTPQQITQELDRFIIGQADAKRAVAI
ALRNRWRRNRVPEPLREEIIPKNILMIGHTGIGKTEIARRLAKLAKAPFIKVEATKFTEIGYVGRDVDSIIRDLVDVAIN
LEKEKSRKFVETKAKSLAENIILEALVGADASQETKTIFQEKLRNGEFENFEISISIKESKNAIPSIDIPNIPGNQVGIM
NINEIVHKMLGNNKQLKTIKVTVKEARELLINEESEKLMDEDKIIKDALLLASNDGIVFLDEIDKIAARTEIRGEVNREG
VQRDLLPLLEGTSVTTKYGTITTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELKPLSKDDLVRILTEPESSLLKQYC
ALMKTENITIDFTDEGVSTIAEIASTVNREVENIGARRLHTILEKLMEDISYTATENSGRTYVIDSEYVKKKLEDIAKQL
DLSKFIL

Sequences:

>Translated_487_residues
MFVTPNNKLKLNNDTTNNINDEQASSEVLNSENAISTEGYEDDINLDDLYNPQELTPQQITQELDRFIIGQADAKRAVAI
ALRNRWRRNRVPEPLREEIIPKNILMIGHTGIGKTEIARRLAKLAKAPFIKVEATKFTEIGYVGRDVDSIIRDLVDVAIN
LEKEKSRKFVETKAKSLAENIILEALVGADASQETKTIFQEKLRNGEFENFEISISIKESKNAIPSIDIPNIPGNQVGIM
NINEIVHKMLGNNKQLKTIKVTVKEARELLINEESEKLMDEDKIIKDALLLASNDGIVFLDEIDKIAARTEIRGEVNREG
VQRDLLPLLEGTSVTTKYGTITTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELKPLSKDDLVRILTEPESSLLKQYC
ALMKTENITIDFTDEGVSTIAEIASTVNREVENIGARRLHTILEKLMEDISYTATENSGRTYVIDSEYVKKKLEDIAKQL
DLSKFIL
>Mature_487_residues
MFVTPNNKLKLNNDTTNNINDEQASSEVLNSENAISTEGYEDDINLDDLYNPQELTPQQITQELDRFIIGQADAKRAVAI
ALRNRWRRNRVPEPLREEIIPKNILMIGHTGIGKTEIARRLAKLAKAPFIKVEATKFTEIGYVGRDVDSIIRDLVDVAIN
LEKEKSRKFVETKAKSLAENIILEALVGADASQETKTIFQEKLRNGEFENFEISISIKESKNAIPSIDIPNIPGNQVGIM
NINEIVHKMLGNNKQLKTIKVTVKEARELLINEESEKLMDEDKIIKDALLLASNDGIVFLDEIDKIAARTEIRGEVNREG
VQRDLLPLLEGTSVTTKYGTITTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELKPLSKDDLVRILTEPESSLLKQYC
ALMKTENITIDFTDEGVSTIAEIASTVNREVENIGARRLHTILEKLMEDISYTATENSGRTYVIDSEYVKKKLEDIAKQL
DLSKFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=53.6912751677852, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1786642, Length=212, Percent_Identity=33.4905660377358, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319704, Length=142, Percent_Identity=33.0985915492958, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648291, Length=242, Percent_Identity=30.1652892561983, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648289, Length=242, Percent_Identity=30.5785123966942, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 54772; Mature: 54772

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTPNNKLKLNNDTTNNINDEQASSEVLNSENAISTEGYEDDINLDDLYNPQELTPQQI
CEECCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
TQELDRFIIGQADAKRAVAIALRNRWRRNRVPEPLREEIIPKNILMIGHTGIGKTEIARR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHH
LAKLAKAPFIKVEATKFTEIGYVGRDVDSIIRDLVDVAINLEKEKSRKFVETKAKSLAEN
HHHHHHCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IILEALVGADASQETKTIFQEKLRNGEFENFEISISIKESKNAIPSIDIPNIPGNQVGIM
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEE
NINEIVHKMLGNNKQLKTIKVTVKEARELLINEESEKLMDEDKIIKDALLLASNDGIVFL
EHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEH
DEIDKIAARTEIRGEVNREGVQRDLLPLLEGTSVTTKYGTITTDHILFIASGAFHLAKPS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCEEECEEEEEECCCCCCCCCH
DLLPELQGRLPIRVELKPLSKDDLVRILTEPESSLLKQYCALMKTENITIDFTDEGVSTI
HHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHH
AEIASTVNREVENIGARRLHTILEKLMEDISYTATENSGRTYVIDSEYVKKKLEDIAKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEECHHHHHHHHHHHHHHH
DLSKFIL
HHHHHCC
>Mature Secondary Structure
MFVTPNNKLKLNNDTTNNINDEQASSEVLNSENAISTEGYEDDINLDDLYNPQELTPQQI
CEECCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
TQELDRFIIGQADAKRAVAIALRNRWRRNRVPEPLREEIIPKNILMIGHTGIGKTEIARR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHH
LAKLAKAPFIKVEATKFTEIGYVGRDVDSIIRDLVDVAINLEKEKSRKFVETKAKSLAEN
HHHHHHCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IILEALVGADASQETKTIFQEKLRNGEFENFEISISIKESKNAIPSIDIPNIPGNQVGIM
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEE
NINEIVHKMLGNNKQLKTIKVTVKEARELLINEESEKLMDEDKIIKDALLLASNDGIVFL
EHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEH
DEIDKIAARTEIRGEVNREGVQRDLLPLLEGTSVTTKYGTITTDHILFIASGAFHLAKPS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCEEECEEEEEECCCCCCCCCH
DLLPELQGRLPIRVELKPLSKDDLVRILTEPESSLLKQYCALMKTENITIDFTDEGVSTI
HHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHH
AEIASTVNREVENIGARRLHTILEKLMEDISYTATENSGRTYVIDSEYVKKKLEDIAKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEECHHHHHHHHHHHHHHH
DLSKFIL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA