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The map label for this gene is purD [H]

Identifier: 88657834

GI number: 88657834

Start: 1032412

End: 1033677

Strand: Direct

Name: purD [H]

Synonym: ECH_1006

Alternate gene names: 88657834

Gene position: 1032412-1033677 (Clockwise)

Preceding gene: 88658064

Following gene: 88658288

Centisome position: 87.77

GC content: 34.99

Gene sequence:

>1266_bases
ATGAATGTATTGGTGATTGGTTCTGGCGGCCGTGAGCACTCAATGTTGCATCACATTCGTAAATCTACATTACTAAACAA
GCTATTTATCGCCCCAGGGCGTGAAGGAATGTCTGGGTTAGCAGATATAATAGATATAGATATCAATAGCACAATAGAAG
TAATTCAAGTATGTAAGAAAGAAAAAATTGAATTAGTAGTCATCGGACCAGAAACTCCATTAATGAATGGATTATCAGAC
GCATTAACAGAAGAAGGCATATTAGTCTTTGGACCTTCTAAAGCAGCAGCGCGTCTTGAATCTTCAAAAGGATTTACAAA
AGAATTATGCATGAGGTATGGAATTCCTACTGCAAAATACGGGTACTTTGTTGATACAAATTCAGCTTACAAATTCATTG
ATAAACACAAATTACCTTTAGTAGTTAAGGCTGATGGGTTAGCCCAGGGAAAAGGAACAGTGATATGTCACACACACGAA
GAAGCATACAATGCTGTAGATGCCATGTTAGTGCACCACAAATTTGGAGAAGCTGGTTGTGCAATAATTATTGAAGAATT
CCTTGAAGGCAAGGAAATTAGCTTCTTTACATTGGTTGACGGATCCAACCCAGTTATACTTGGCGTAGCACAAGATTATA
AAACTATAGGAGATAATAATAAAGGTCCTAATACTGGAGGGATGGGATCATACTCTAAACCAAATATCATTACACAAGAA
ATGGAGCATATAATAATTCAGAAGATAATATATCCAACTATTAAAGCAATGTTCAACATGAATATACAGTTTAGAGGTCT
GTTATTCGCTGGTATTATAATCAAAAAAAATGAACCAAAATTACTTGAATATAATGTACGGTTTGGAGATCCTGAAACAC
AATCAATATTACCAAGATTAAATTCCGATTTCTTAAAACTTTTATCACTAACAGCTAAAGGTAAACTAGGAAATGAATCA
GTAGAATTAAGTAAAAAAGCTGCTTTATGTGTTGTGGTAGCTAGTCGTGGATATCCAGGTGAGTATAAGAAAAATTCTAT
AATTAATGGAATAGAAAATATTGAAAAGCTACCTAATGTTCAGCTCTTACATGCAGGCACAAGAAGAGAAGGAAATAACT
GGGTATCAGATTCTGGAAGAGTAATAAATGTTGTAGCACAAGGTGAAAATTTAGCTAGTGCGAAACACCAAGCCTACGCT
GCATTGGACTTATTAGATTGGCCAGATGGAATTTACAGATATGATATAGGATCATGTGCTCTTTAA

Upstream 100 bases:

>100_bases
ATTTTTACTAATGTACTTACACTAGCAAGACCATATAATGTATTGACAACTATCATATTGATACTAGAATGCAATAAGTA
CAAACTAATAAAGCATACTA

Downstream 100 bases:

>100_bases
GCTATACTCTATGAATATTTAGCATAAAATTAGATAAAGCACGATTTAATTGTGCAAGCCGATTTTTGAATATTACAAAT
CTTGATCCAGAAAGTTTTGT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNVLVIGSGGREHSMLHHIRKSTLLNKLFIAPGREGMSGLADIIDIDINSTIEVIQVCKKEKIELVVIGPETPLMNGLSD
ALTEEGILVFGPSKAAARLESSKGFTKELCMRYGIPTAKYGYFVDTNSAYKFIDKHKLPLVVKADGLAQGKGTVICHTHE
EAYNAVDAMLVHHKFGEAGCAIIIEEFLEGKEISFFTLVDGSNPVILGVAQDYKTIGDNNKGPNTGGMGSYSKPNIITQE
MEHIIIQKIIYPTIKAMFNMNIQFRGLLFAGIIIKKNEPKLLEYNVRFGDPETQSILPRLNSDFLKLLSLTAKGKLGNES
VELSKKAALCVVVASRGYPGEYKKNSIINGIENIEKLPNVQLLHAGTRREGNNWVSDSGRVINVVAQGENLASAKHQAYA
ALDLLDWPDGIYRYDIGSCAL

Sequences:

>Translated_421_residues
MNVLVIGSGGREHSMLHHIRKSTLLNKLFIAPGREGMSGLADIIDIDINSTIEVIQVCKKEKIELVVIGPETPLMNGLSD
ALTEEGILVFGPSKAAARLESSKGFTKELCMRYGIPTAKYGYFVDTNSAYKFIDKHKLPLVVKADGLAQGKGTVICHTHE
EAYNAVDAMLVHHKFGEAGCAIIIEEFLEGKEISFFTLVDGSNPVILGVAQDYKTIGDNNKGPNTGGMGSYSKPNIITQE
MEHIIIQKIIYPTIKAMFNMNIQFRGLLFAGIIIKKNEPKLLEYNVRFGDPETQSILPRLNSDFLKLLSLTAKGKLGNES
VELSKKAALCVVVASRGYPGEYKKNSIINGIENIEKLPNVQLLHAGTRREGNNWVSDSGRVINVVAQGENLASAKHQAYA
ALDLLDWPDGIYRYDIGSCAL
>Mature_421_residues
MNVLVIGSGGREHSMLHHIRKSTLLNKLFIAPGREGMSGLADIIDIDINSTIEVIQVCKKEKIELVVIGPETPLMNGLSD
ALTEEGILVFGPSKAAARLESSKGFTKELCMRYGIPTAKYGYFVDTNSAYKFIDKHKLPLVVKADGLAQGKGTVICHTHE
EAYNAVDAMLVHHKFGEAGCAIIIEEFLEGKEISFFTLVDGSNPVILGVAQDYKTIGDNNKGPNTGGMGSYSKPNIITQE
MEHIIIQKIIYPTIKAMFNMNIQFRGLLFAGIIIKKNEPKLLEYNVRFGDPETQSILPRLNSDFLKLLSLTAKGKLGNES
VELSKKAALCVVVASRGYPGEYKKNSIINGIENIEKLPNVQLLHAGTRREGNNWVSDSGRVINVVAQGENLASAKHQAYA
ALDLLDWPDGIYRYDIGSCAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=420, Percent_Identity=40.7142857142857, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI4503915, Length=420, Percent_Identity=40.7142857142857, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI209869995, Length=420, Percent_Identity=40.7142857142857, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI209869993, Length=420, Percent_Identity=40.7142857142857, Blast_Score=305, Evalue=5e-83,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=40.4705882352941, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=37.2037914691943, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=37.0454545454545, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=36.7441860465116, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=36.7441860465116, Blast_Score=251, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46106; Mature: 46106

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGSGGREHSMLHHIRKSTLLNKLFIAPGREGMSGLADIIDIDINSTIEVIQVCKK
CEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHEEEECCCCHHHHHHHHHH
EKIELVVIGPETPLMNGLSDALTEEGILVFGPSKAAARLESSKGFTKELCMRYGIPTAKY
CCEEEEEECCCCCHHHCHHHHHHHCCEEEECCCHHHHHHHCCCCHHHHHHHHHCCCCCCC
GYFVDTNSAYKFIDKHKLPLVVKADGLAQGKGTVICHTHEEAYNAVDAMLVHHKFGEAGC
CEEEECCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCH
AIIIEEFLEGKEISFFTLVDGSNPVILGVAQDYKTIGDNNKGPNTGGMGSYSKPNIITQE
HHHHHHHHCCCCEEEEEEECCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
MEHIIIQKIIYPTIKAMFNMNIQFRGLLFAGIIIKKNEPKLLEYNVRFGDPETQSILPRL
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHC
NSDFLKLLSLTAKGKLGNESVELSKKAALCVVVASRGYPGEYKKNSIINGIENIEKLPNV
CHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCE
QLLHAGTRREGNNWVSDSGRVINVVAQGENLASAKHQAYAALDLLDWPDGIYRYDIGSCA
EEEECCCCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHEEEEEECCCCCCEEECCCCCC
L
C
>Mature Secondary Structure
MNVLVIGSGGREHSMLHHIRKSTLLNKLFIAPGREGMSGLADIIDIDINSTIEVIQVCKK
CEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHEEEECCCCHHHHHHHHHH
EKIELVVIGPETPLMNGLSDALTEEGILVFGPSKAAARLESSKGFTKELCMRYGIPTAKY
CCEEEEEECCCCCHHHCHHHHHHHCCEEEECCCHHHHHHHCCCCHHHHHHHHHCCCCCCC
GYFVDTNSAYKFIDKHKLPLVVKADGLAQGKGTVICHTHEEAYNAVDAMLVHHKFGEAGC
CEEEECCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCH
AIIIEEFLEGKEISFFTLVDGSNPVILGVAQDYKTIGDNNKGPNTGGMGSYSKPNIITQE
HHHHHHHHCCCCEEEEEEECCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
MEHIIIQKIIYPTIKAMFNMNIQFRGLLFAGIIIKKNEPKLLEYNVRFGDPETQSILPRL
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHC
NSDFLKLLSLTAKGKLGNESVELSKKAALCVVVASRGYPGEYKKNSIINGIENIEKLPNV
CHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCE
QLLHAGTRREGNNWVSDSGRVINVVAQGENLASAKHQAYAALDLLDWPDGIYRYDIGSCA
EEEECCCCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHEEEEEECCCCCCEEECCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]