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The map label for this gene is nuoI

Identifier: 88657739

GI number: 88657739

Start: 700112

End: 700621

Strand: Direct

Name: nuoI

Synonym: ECH_0691

Alternate gene names: 88657739

Gene position: 700112-700621 (Clockwise)

Preceding gene: 88658130

Following gene: 88657646

Centisome position: 59.52

GC content: 34.31

Gene sequence:

>510_bases
ATGATTTATAAGAAAAATTTTTCAAAGTTATCACCTATCACAAATTTGATACGTAGTGCTCTTCTTAACTGTAGGGGTAT
GTACATTACTTTACGTTATATGTTTAAGCCAAAAGTTACTTTAAACTATCCATTAGAAAAAGGTCCCTTAAGTACAAGGT
TTAGAGGAGAACATGCATTACGCAAGTATAAAAATGGAGAAGAACGTTGTATAGCATGCAAATTGTGTGAAGCTATATGT
CCAGCTCAAGCAATCACAATTGAAGCTCAAGAACGAGATGATGGTAGTCGCCGTACAGTACGCTATGATATAGACATGAC
AAAATGCATATATTGCGGTTTCTGCCAAGAAGCATGCCCTGTAGATGCAATAGTAGAAGGTCCAAATTTTGAATATGCAA
CAGAAACAAGAGAGGAGCTTATGTACAACAAAAGTAAATTACTCCATAATGGACAAATATGGGAAGAAGCCATTGATTTA
AGGATCAAAAAGAATAGCCAATTTTATTAA

Upstream 100 bases:

>100_bases
AAGCTCATGTGGTAGCACTGTCATTTAAAAAGTCTGATACTGTTTTACCTTTTGCAATAAGGTTAGATAGAATATGGCAT
ACTATTAATTAGGCCTATTT

Downstream 100 bases:

>100_bases
TAAAATCAAATTTTTAAGTATATATAATCATCTTCTACATTAAAAATTCTGTCAATTATTACGATATACTTTTAAAATCA
GTTATAACGTTTTATATTTT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MIYKKNFSKLSPITNLIRSALLNCRGMYITLRYMFKPKVTLNYPLEKGPLSTRFRGEHALRKYKNGEERCIACKLCEAIC
PAQAITIEAQERDDGSRRTVRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYATETREELMYNKSKLLHNGQIWEEAIDL
RIKKNSQFY

Sequences:

>Translated_169_residues
MIYKKNFSKLSPITNLIRSALLNCRGMYITLRYMFKPKVTLNYPLEKGPLSTRFRGEHALRKYKNGEERCIACKLCEAIC
PAQAITIEAQERDDGSRRTVRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYATETREELMYNKSKLLHNGQIWEEAIDL
RIKKNSQFY
>Mature_169_residues
MIYKKNFSKLSPITNLIRSALLNCRGMYITLRYMFKPKVTLNYPLEKGPLSTRFRGEHALRKYKNGEERCIACKLCEAIC
PAQAITIEAQERDDGSRRTVRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYATETREELMYNKSKLLHNGQIWEEAIDL
RIKKNSQFY

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=141, Percent_Identity=75.177304964539, Blast_Score=231, Evalue=2e-61,
Organism=Escherichia coli, GI1788617, Length=127, Percent_Identity=37.7952755905512, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789075, Length=128, Percent_Identity=28.125, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=131, Percent_Identity=77.8625954198473, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI17864306, Length=141, Percent_Identity=75.177304964539, Blast_Score=231, Evalue=2e-61,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_EHRCR (Q2GGD7)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507496.1
- HSSP:   Q9I6D2
- ProteinModelPortal:   Q2GGD7
- SMR:   Q2GGD7
- STRING:   Q2GGD7
- GeneID:   3927536
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0691
- TIGR:   ECH_0691
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- PhylomeDB:   Q2GGD7
- ProtClustDB:   PRK05888
- BioCyc:   ECHA205920:ECH_0691-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 19700; Mature: 19700

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYKKNFSKLSPITNLIRSALLNCRGMYITLRYMFKPKVTLNYPLEKGPLSTRFRGEHAL
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHH
RKYKNGEERCIACKLCEAICPAQAITIEAQERDDGSRRTVRYDIDMTKCIYCGFCQEACP
HHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCCCC
VDAIVEGPNFEYATETREELMYNKSKLLHNGQIWEEAIDLRIKKNSQFY
CCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCC
>Mature Secondary Structure
MIYKKNFSKLSPITNLIRSALLNCRGMYITLRYMFKPKVTLNYPLEKGPLSTRFRGEHAL
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHH
RKYKNGEERCIACKLCEAICPAQAITIEAQERDDGSRRTVRYDIDMTKCIYCGFCQEACP
HHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCCCC
VDAIVEGPNFEYATETREELMYNKSKLLHNGQIWEEAIDLRIKKNSQFY
CCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA