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The map label for this gene is lepB [H]

Identifier: 88658130

GI number: 88658130

Start: 699396

End: 700103

Strand: Direct

Name: lepB [H]

Synonym: ECH_0690

Alternate gene names: 88658130

Gene position: 699396-700103 (Clockwise)

Preceding gene: 88657741

Following gene: 88657739

Centisome position: 59.46

GC content: 34.32

Gene sequence:

>708_bases
TTGAAAAAATATTACTCTTTCATAAGGCTTTTTTCAACTGTATTTCTTGCCTTAGTTGTAGCTCTATTGCTGCGGGTCTT
TTTATTTGAACCATTCCACATACCTTCCGGTTCAATGAAAAGTACATTGTTAGTAGGTGACTATATATTTATCAGTAAGT
ATAGCTATGGTTATAGCAAGCATTCTATTCCCTTTTCACTACCAATAATAAAAGGTCGCATTTTTTCTAAACTACCAAAA
GCTGGTGATGTTGTTGTATTTCGTCCACCTAAAGAGATGAATCTTCATTATATCAAAAGAGTCATTGGAGTACCTGGAGA
TAAAATACAGCTCATTGATGGGTTTTTGTATATTAATGGAGAAAAAATGAAGTATGAAAAGCTGGGTGATTTTGTTGATG
ATGACGGAAAGGTAATTAGCCGCTATTCAGAAACTCTATATAATGGAAATACTCATGAAATATTAGACGAAGTTCCAGGT
AGCCCACTTGACAACACTCCTGTATATCATGTACCTGAAGGGTATGTTTTTGTACTAGGAGATAATAGGGATAATTCCCG
AGATAGCAGGTTTATTACAGATGTTGGATACATCCCTCTTGAAAACATCGTAGGTAAAGCTCATGTGGTAGCACTGTCAT
TTAAAAAGTCTGATACTGTTTTACCTTTTGCAATAAGGTTAGATAGAATATGGCATACTATTAATTAG

Upstream 100 bases:

>100_bases
TTGAAGATATTAATGGGTCAAGATTTATAATCAACAACCCAATTGCAAAATCTAAGTGTGGATGTGGTAATAGTTTCTCT
ATATAAGTCATGGTATAACT

Downstream 100 bases:

>100_bases
GCCTATTTATGATTTATAAGAAAAATTTTTCAAAGTTATCACCTATCACAAATTTGATACGTAGTGCTCTTCTTAACTGT
AGGGGTATGTACATTACTTT

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKKYYSFIRLFSTVFLALVVALLLRVFLFEPFHIPSGSMKSTLLVGDYIFISKYSYGYSKHSIPFSLPIIKGRIFSKLPK
AGDVVVFRPPKEMNLHYIKRVIGVPGDKIQLIDGFLYINGEKMKYEKLGDFVDDDGKVISRYSETLYNGNTHEILDEVPG
SPLDNTPVYHVPEGYVFVLGDNRDNSRDSRFITDVGYIPLENIVGKAHVVALSFKKSDTVLPFAIRLDRIWHTIN

Sequences:

>Translated_235_residues
MKKYYSFIRLFSTVFLALVVALLLRVFLFEPFHIPSGSMKSTLLVGDYIFISKYSYGYSKHSIPFSLPIIKGRIFSKLPK
AGDVVVFRPPKEMNLHYIKRVIGVPGDKIQLIDGFLYINGEKMKYEKLGDFVDDDGKVISRYSETLYNGNTHEILDEVPG
SPLDNTPVYHVPEGYVFVLGDNRDNSRDSRFITDVGYIPLENIVGKAHVVALSFKKSDTVLPFAIRLDRIWHTIN
>Mature_235_residues
MKKYYSFIRLFSTVFLALVVALLLRVFLFEPFHIPSGSMKSTLLVGDYIFISKYSYGYSKHSIPFSLPIIKGRIFSKLPK
AGDVVVFRPPKEMNLHYIKRVIGVPGDKIQLIDGFLYINGEKMKYEKLGDFVDDDGKVISRYSETLYNGNTHEILDEVPG
SPLDNTPVYHVPEGYVFVLGDNRDNSRDSRFITDVGYIPLENIVGKAHVVALSFKKSDTVLPFAIRLDRIWHTIN

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=276, Percent_Identity=33.695652173913, Blast_Score=130, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 26804; Mature: 26804

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYYSFIRLFSTVFLALVVALLLRVFLFEPFHIPSGSMKSTLLVGDYIFISKYSYGYSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECEEEEEEECCCCCC
HSIPFSLPIIKGRIFSKLPKAGDVVVFRPPKEMNLHYIKRVIGVPGDKIQLIDGFLYING
CCCCEECHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEEECC
EKMKYEKLGDFVDDDGKVISRYSETLYNGNTHEILDEVPGSPLDNTPVYHVPEGYVFVLG
CEEEHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEEE
DNRDNSRDSRFITDVGYIPLENIVGKAHVVALSFKKSDTVLPFAIRLDRIWHTIN
CCCCCCCCCEEEEECCCCCHHHHCCCEEEEEEEECCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKYYSFIRLFSTVFLALVVALLLRVFLFEPFHIPSGSMKSTLLVGDYIFISKYSYGYSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECEEEEEEECCCCCC
HSIPFSLPIIKGRIFSKLPKAGDVVVFRPPKEMNLHYIKRVIGVPGDKIQLIDGFLYING
CCCCEECHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEEECC
EKMKYEKLGDFVDDDGKVISRYSETLYNGNTHEILDEVPGSPLDNTPVYHVPEGYVFVLG
CEEEHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEEE
DNRDNSRDSRFITDVGYIPLENIVGKAHVVALSFKKSDTVLPFAIRLDRIWHTIN
CCCCCCCCCEEEEECCCCCHHHHCCCEEEEEEEECCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA