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The map label for this gene is tig

Identifier: 88657614

GI number: 88657614

Start: 929517

End: 930836

Strand: Reverse

Name: tig

Synonym: ECH_0902

Alternate gene names: 88657614

Gene position: 930836-929517 (Counterclockwise)

Preceding gene: 88658563

Following gene: 88658316

Centisome position: 79.14

GC content: 30.53

Gene sequence:

>1320_bases
ATGCTGAATAGTTATGTAGTTAGAGAAGTTTCAAATGATAAATTGAAGTGGGAATATGAATTTGCTGTTGATAAAAAGTA
TTTTTTAGACCAGTTAGATTCTAAGTTGTCAGAGATTGCTATGAATGTAAAGGTACCTGGCTTTAGAGTAGGAAAGGCTT
CAATTGATTTAGTAAGGAAAGAATATTTAAATGAAGCTATGACTAGTGTAGTGAAGAAGACTATTGAAAGTACGTCTTCT
GATTTTGTTAAAAATAGTAAATTTGGAGAGATAATATCATCGAATATTGATATTGTTTCTTATCCTAGCTATTACTCTGA
TAATGATAAAGAAGAAGATTTAGTCTATAAACTGAGTTTTGAGGTTATGCCTGAAGCTCCATTAATGGATATTGATAATA
TAGTTTTAAGCGATATAGAAGTTGATATACAAGAGTGTGATGTCAATGAATTTATAGAAAATTTAAAAAAACAAAGACCT
GATTTTGTTATAGTGGATGATCCGGAATATGTAATTCAAGGCAGTGATAAATTAGTTATTGATTATCAAAATAAAATAAA
AGGTAAAATCTTGAGAGGCGGTTCTGCCAAGGACTTTGTGTTGGTGTTAGGTAAAGGTGTAGCTTTAAGAGAATTTGAAG
ATCAGTTGATAGGTATGAAAGTTGGGGAATCTAAAACTTTTCCTCTAACTTTCCCTAACGATTATGGTATGGTACATCTT
GCTGGAAAAACTACAGATATGTCAGTTACTGTTAAAAGTGTATATGTTATGAAAGGTATGCGAGATAGTGAAGCTATAGC
AAAAGATTATGGCTTTAAAGATGTAGGTCATATGGAAGATTTTGCTAGGAAAAGAATAAAGCAACAATTTGATCAAATGG
TTTTCACTATAGTGAAGAAAGAATTATTTGATTATATGGATGCTAACTATGTTATAGATGTACCAGAGTGTGTTGTTACT
CAAGAAATTGCAAAAATAAACAAGGAAATACGTGATTCTGGAGAAGATATACAAATTGATGTAGAGAAGGAAGCAATAAA
GCGAGTTAAGCTTGGTATGTTACTGATAAAGATGTCTCGTCATAATAATATTACTATTAAAAATGAAGATGTTTTTAGCT
TCATACAGAGCAATTATGCTGATTATGGAGTAGATATTGGTAATGTGTTGAAAATGCTGCAATCTAATAAAAATTTTGCT
AATTATATTAGTGGGAAAGTACTTGAAGAAAAGGTCATTAACTATATAATAGGGCTGGCCAAAAAGGACAAGAAGGTTAT
GACAGCTAAAGATATAAGCCTTATGTTTGAGAATATATAG

Upstream 100 bases:

>100_bases
CGGAATTGGTAGACGTGCTAGGTTTAGGTCCTAGTAAGGAAACTTGTGGGGGTTCAAGTCCCTCTATCCGCACTTTATAT
ATCAGTAAGGGTATTGTAAT

Downstream 100 bases:

>100_bases
TTGTAAAATTATATTAAATGGATTAGTTTAAGACAGCATTTATAGTGATTGTAAAGAAAAGGAGATGGCTATGACTTTAG
TCCCCATGGTAGTTGAACAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLNSYVVREVSNDKLKWEYEFAVDKKYFLDQLDSKLSEIAMNVKVPGFRVGKASIDLVRKEYLNEAMTSVVKKTIESTSS
DFVKNSKFGEIISSNIDIVSYPSYYSDNDKEEDLVYKLSFEVMPEAPLMDIDNIVLSDIEVDIQECDVNEFIENLKKQRP
DFVIVDDPEYVIQGSDKLVIDYQNKIKGKILRGGSAKDFVLVLGKGVALREFEDQLIGMKVGESKTFPLTFPNDYGMVHL
AGKTTDMSVTVKSVYVMKGMRDSEAIAKDYGFKDVGHMEDFARKRIKQQFDQMVFTIVKKELFDYMDANYVIDVPECVVT
QEIAKINKEIRDSGEDIQIDVEKEAIKRVKLGMLLIKMSRHNNITIKNEDVFSFIQSNYADYGVDIGNVLKMLQSNKNFA
NYISGKVLEEKVINYIIGLAKKDKKVMTAKDISLMFENI

Sequences:

>Translated_439_residues
MLNSYVVREVSNDKLKWEYEFAVDKKYFLDQLDSKLSEIAMNVKVPGFRVGKASIDLVRKEYLNEAMTSVVKKTIESTSS
DFVKNSKFGEIISSNIDIVSYPSYYSDNDKEEDLVYKLSFEVMPEAPLMDIDNIVLSDIEVDIQECDVNEFIENLKKQRP
DFVIVDDPEYVIQGSDKLVIDYQNKIKGKILRGGSAKDFVLVLGKGVALREFEDQLIGMKVGESKTFPLTFPNDYGMVHL
AGKTTDMSVTVKSVYVMKGMRDSEAIAKDYGFKDVGHMEDFARKRIKQQFDQMVFTIVKKELFDYMDANYVIDVPECVVT
QEIAKINKEIRDSGEDIQIDVEKEAIKRVKLGMLLIKMSRHNNITIKNEDVFSFIQSNYADYGVDIGNVLKMLQSNKNFA
NYISGKVLEEKVINYIIGLAKKDKKVMTAKDISLMFENI
>Mature_439_residues
MLNSYVVREVSNDKLKWEYEFAVDKKYFLDQLDSKLSEIAMNVKVPGFRVGKASIDLVRKEYLNEAMTSVVKKTIESTSS
DFVKNSKFGEIISSNIDIVSYPSYYSDNDKEEDLVYKLSFEVMPEAPLMDIDNIVLSDIEVDIQECDVNEFIENLKKQRP
DFVIVDDPEYVIQGSDKLVIDYQNKIKGKILRGGSAKDFVLVLGKGVALREFEDQLIGMKVGESKTFPLTFPNDYGMVHL
AGKTTDMSVTVKSVYVMKGMRDSEAIAKDYGFKDVGHMEDFARKRIKQQFDQMVFTIVKKELFDYMDANYVIDVPECVVT
QEIAKINKEIRDSGEDIQIDVEKEAIKRVKLGMLLIKMSRHNNITIKNEDVFSFIQSNYADYGVDIGNVLKMLQSNKNFA
NYISGKVLEEKVINYIIGLAKKDKKVMTAKDISLMFENI

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=407, Percent_Identity=26.2899262899263, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_EHRCR (Q2GFT7)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507696.1
- ProteinModelPortal:   Q2GFT7
- SMR:   Q2GFT7
- STRING:   Q2GFT7
- GeneID:   3927488
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0902
- TIGR:   ECH_0902
- eggNOG:   COG0544
- HOGENOM:   HBG311097
- OMA:   FEGRIRN
- PhylomeDB:   Q2GFT7
- ProtClustDB:   CLSK749502
- BioCyc:   ECHA205920:ECH_0902-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 50188; Mature: 50188

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSYVVREVSNDKLKWEYEFAVDKKYFLDQLDSKLSEIAMNVKVPGFRVGKASIDLVRK
CCCCCEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHEEEEECCCEEECHHHHHHHHH
EYLNEAMTSVVKKTIESTSSDFVKNSKFGEIISSNIDIVSYPSYYSDNDKEEDLVYKLSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEE
EVMPEAPLMDIDNIVLSDIEVDIQECDVNEFIENLKKQRPDFVIVDDPEYVIQGSDKLVI
EECCCCCCCCCCHHHHHHHEEEHHHCCHHHHHHHHHHCCCCEEEECCCCEEEECCCEEEE
DYQNKIKGKILRGGSAKDFVLVLGKGVALREFEDQLIGMKVGESKTFPLTFPNDYGMVHL
EECCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHCCEEECCCCCCEEECCCCCCEEEE
AGKTTDMSVTVKSVYVMKGMRDSEAIAKDYGFKDVGHMEDFARKRIKQQFDQMVFTIVKK
ECCCCCCEEEHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELFDYMDANYVIDVPECVVTQEIAKINKEIRDSGEDIQIDVEKEAIKRVKLGMLLIKMSR
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHEEEEEC
HNNITIKNEDVFSFIQSNYADYGVDIGNVLKMLQSNKNFANYISGKVLEEKVINYIIGLA
CCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
KKDKKVMTAKDISLMFENI
CCCCCEEHHHHHHHHHCCC
>Mature Secondary Structure
MLNSYVVREVSNDKLKWEYEFAVDKKYFLDQLDSKLSEIAMNVKVPGFRVGKASIDLVRK
CCCCCEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHEEEEECCCEEECHHHHHHHHH
EYLNEAMTSVVKKTIESTSSDFVKNSKFGEIISSNIDIVSYPSYYSDNDKEEDLVYKLSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEE
EVMPEAPLMDIDNIVLSDIEVDIQECDVNEFIENLKKQRPDFVIVDDPEYVIQGSDKLVI
EECCCCCCCCCCHHHHHHHEEEHHHCCHHHHHHHHHHCCCCEEEECCCCEEEECCCEEEE
DYQNKIKGKILRGGSAKDFVLVLGKGVALREFEDQLIGMKVGESKTFPLTFPNDYGMVHL
EECCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHCCEEECCCCCCEEECCCCCCEEEE
AGKTTDMSVTVKSVYVMKGMRDSEAIAKDYGFKDVGHMEDFARKRIKQQFDQMVFTIVKK
ECCCCCCEEEHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELFDYMDANYVIDVPECVVTQEIAKINKEIRDSGEDIQIDVEKEAIKRVKLGMLLIKMSR
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHEEEEEC
HNNITIKNEDVFSFIQSNYADYGVDIGNVLKMLQSNKNFANYISGKVLEEKVINYIIGLA
CCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
KKDKKVMTAKDISLMFENI
CCCCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA