Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 88607953

GI number: 88607953

Start: 388949

End: 390046

Strand: Reverse

Name: carA [H]

Synonym: APH_0381

Alternate gene names: 88607953

Gene position: 390046-388949 (Counterclockwise)

Preceding gene: 88606996

Following gene: 88607179

Centisome position: 26.51

GC content: 45.72

Gene sequence:

>1098_bases
ATGACCAGCGAATTTCATAATGCGGTTATTGTACTGTCTGATGGGCGGTATTTTTTCGGAAGATCACTCGGTAAGAGGAC
TGACTGCGTTGGAGAGGTGTGCTTCACTACCGGAGTCACAGGATATCAGTATACTATTACTGATCCTTCCTTTGCCGGTC
AGATCGTCACGTTCACGTTTCCTCACATAGGGAATGTCGGGATAAATAGCAAGGATTTTGAGTGTGAGCAGGTCTTGGCT
AAGGGCATAGTAGTTAGGGAGATATCCGGTGCGTCGCATGTATCTTCACATATAGATCTTAACTCTTGGATAGAGCAAAA
TAATTTCACCGGGATATCTGGTGTAGACACCAGGGCGTTAGCCATACACTTGCGCGAGAATGGTACACTAGGTGGAGTCA
TACATCACTTCGACGATATAGGTGCGGTGCACATACAGGAATTACAAAATGTGGCACGTACGCATAAAAAGTCGCTGGCA
ACTGAGCTCCCTCACCTTATGGATCATAGCCATCATGTTGATGGGCACAGTGTTTGCGTCATTGATTTTGGTGCAAAACA
TGGAATATTTAGCGCCTTGACTAGAATAGGGTGCGCTGTTCATGTGGTAGCCGCGCAGAATGACTTTGCAGCCAGAGCCT
TGTCACTAAAACCCAGAGGCATAGTAATATCAAATGGCCCTGGAGATCCCGCAGATATAAGTGACCCCGTTATGGACCAA
TTACGCGTGCTTATAGATTCTGGAATCCCTGTTTTGGGCATATGTTTGGGACATCAACTTATAGCAAAGGCTCTTGGAGC
CAAAACTATTAAGATGCACCATGGTCATAGGGGAAGCAACCATCCAGTATATAGCCTGGAAACTAAGTCTGTAGAAGTCA
CTAGCCAAAACCATGGGTATACCGTTGACGCCAGTACTTTGCCCGATAACATAGAGGTCACACACATATCACTTTTTGAT
GGCACCGTAGAGGGAATCAAGATGACCAATCGTCCTGTGCTTTCGGTGCAATATCATCCAGAGGGTTGCCCAGGACCTAA
CGATTCGCACTATATTTTCAGAAAGTTTGCCGATTTGATCACATCTGCACACGCATAA

Upstream 100 bases:

>100_bases
TCTGTTTCTCCTCACATATTATTATGAAAGTTTTTACACAAATCTCTATCAGTTTCTTAAGAGCTTTGGTACAATACCGG
AGTTTGACTTTCATGCAAGT

Downstream 100 bases:

>100_bases
CTGCTTTAGAGCCTAGTGCTTCGCTGTGCTTGACTCTATGGGTTTCTAGTGACTTAGAAAGTCTGCTGATTTTGATCACA
TCAAAGCATGTGTGCCTGTT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTSEFHNAVIVLSDGRYFFGRSLGKRTDCVGEVCFTTGVTGYQYTITDPSFAGQIVTFTFPHIGNVGINSKDFECEQVLA
KGIVVREISGASHVSSHIDLNSWIEQNNFTGISGVDTRALAIHLRENGTLGGVIHHFDDIGAVHIQELQNVARTHKKSLA
TELPHLMDHSHHVDGHSVCVIDFGAKHGIFSALTRIGCAVHVVAAQNDFAARALSLKPRGIVISNGPGDPADISDPVMDQ
LRVLIDSGIPVLGICLGHQLIAKALGAKTIKMHHGHRGSNHPVYSLETKSVEVTSQNHGYTVDASTLPDNIEVTHISLFD
GTVEGIKMTNRPVLSVQYHPEGCPGPNDSHYIFRKFADLITSAHA

Sequences:

>Translated_365_residues
MTSEFHNAVIVLSDGRYFFGRSLGKRTDCVGEVCFTTGVTGYQYTITDPSFAGQIVTFTFPHIGNVGINSKDFECEQVLA
KGIVVREISGASHVSSHIDLNSWIEQNNFTGISGVDTRALAIHLRENGTLGGVIHHFDDIGAVHIQELQNVARTHKKSLA
TELPHLMDHSHHVDGHSVCVIDFGAKHGIFSALTRIGCAVHVVAAQNDFAARALSLKPRGIVISNGPGDPADISDPVMDQ
LRVLIDSGIPVLGICLGHQLIAKALGAKTIKMHHGHRGSNHPVYSLETKSVEVTSQNHGYTVDASTLPDNIEVTHISLFD
GTVEGIKMTNRPVLSVQYHPEGCPGPNDSHYIFRKFADLITSAHA
>Mature_364_residues
TSEFHNAVIVLSDGRYFFGRSLGKRTDCVGEVCFTTGVTGYQYTITDPSFAGQIVTFTFPHIGNVGINSKDFECEQVLAK
GIVVREISGASHVSSHIDLNSWIEQNNFTGISGVDTRALAIHLRENGTLGGVIHHFDDIGAVHIQELQNVARTHKKSLAT
ELPHLMDHSHHVDGHSVCVIDFGAKHGIFSALTRIGCAVHVVAAQNDFAARALSLKPRGIVISNGPGDPADISDPVMDQL
RVLIDSGIPVLGICLGHQLIAKALGAKTIKMHHGHRGSNHPVYSLETKSVEVTSQNHGYTVDASTLPDNIEVTHISLFDG
TVEGIKMTNRPVLSVQYHPEGCPGPNDSHYIFRKFADLITSAHA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=34.8648648648649, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI169790915, Length=382, Percent_Identity=33.7696335078534, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI21361331, Length=382, Percent_Identity=33.7696335078534, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=42.627345844504, Blast_Score=284, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI193204318, Length=381, Percent_Identity=32.8083989501312, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6324878, Length=378, Percent_Identity=35.978835978836, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6322331, Length=388, Percent_Identity=31.1855670103093, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45555749, Length=377, Percent_Identity=34.4827586206897, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24642586, Length=374, Percent_Identity=34.7593582887701, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39452; Mature: 39321

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEFHNAVIVLSDGRYFFGRSLGKRTDCVGEVCFTTGVTGYQYTITDPSFAGQIVTFTF
CCCCCCCEEEEEECCCEEEHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEC
PHIGNVGINSKDFECEQVLAKGIVVREISGASHVSSHIDLNSWIEQNNFTGISGVDTRAL
CCCCCCCCCCCCCHHHHHHHCCEEEEECCCCHHHHHCCCHHHHHHCCCCCCCCCCCCEEE
AIHLRENGTLGGVIHHFDDIGAVHIQELQNVARTHKKSLATELPHLMDHSHHVDGHSVCV
EEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
IDFGAKHGIFSALTRIGCAVHVVAAQNDFAARALSLKPRGIVISNGPGDPADISDPVMDQ
EECCCCCCHHHHHHHCCEEEEEEEECCCHHHEEEEECCCEEEEECCCCCCCCCCHHHHHH
LRVLIDSGIPVLGICLGHQLIAKALGAKTIKMHHGHRGSNHPVYSLETKSVEVTSQNHGY
HHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCEEEEEECCCCE
TVDASTLPDNIEVTHISLFDGTVEGIKMTNRPVLSVQYHPEGCPGPNDSHYIFRKFADLI
EEECCCCCCCEEEEEEEEECCCCCCEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
TSAHA
HHCCC
>Mature Secondary Structure 
TSEFHNAVIVLSDGRYFFGRSLGKRTDCVGEVCFTTGVTGYQYTITDPSFAGQIVTFTF
CCCCCCEEEEEECCCEEEHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEC
PHIGNVGINSKDFECEQVLAKGIVVREISGASHVSSHIDLNSWIEQNNFTGISGVDTRAL
CCCCCCCCCCCCCHHHHHHHCCEEEEECCCCHHHHHCCCHHHHHHCCCCCCCCCCCCEEE
AIHLRENGTLGGVIHHFDDIGAVHIQELQNVARTHKKSLATELPHLMDHSHHVDGHSVCV
EEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
IDFGAKHGIFSALTRIGCAVHVVAAQNDFAARALSLKPRGIVISNGPGDPADISDPVMDQ
EECCCCCCHHHHHHHCCEEEEEEEECCCHHHEEEEECCCEEEEECCCCCCCCCCHHHHHH
LRVLIDSGIPVLGICLGHQLIAKALGAKTIKMHHGHRGSNHPVYSLETKSVEVTSQNHGY
HHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCEEEEEECCCCE
TVDASTLPDNIEVTHISLFDGTVEGIKMTNRPVLSVQYHPEGCPGPNDSHYIFRKFADLI
EEECCCCCCCEEEEEEEEECCCCCCEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
TSAHA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA