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The map label for this gene is ispH [H]

Identifier: 88607179

GI number: 88607179

Start: 387407

End: 388381

Strand: Reverse

Name: ispH [H]

Synonym: APH_0380

Alternate gene names: 88607179

Gene position: 388381-387407 (Counterclockwise)

Preceding gene: 88607953

Following gene: 88606839

Centisome position: 26.4

GC content: 42.77

Gene sequence:

>975_bases
ATGCATAGTAACTGCAATGACGTTGAGGTGATTTTAGCGCAACCGCGAGGCTTTTGTGCCGGTGTGGAGAGAGCGGTAGC
TATTCTTGATATGGTTGTTGACCAGTATATTGCTAGTAAAGAAGTTTACGTTCTGCATGAGATTGTCCACAACGCCTATG
TGGTGGAAAGTTTTAGAAAAAGAAACGTTAAGTTCGTAGCCTCACTTGATGAAGTTCCTACTGGAGCTGTTCTTGTGTTC
AGTGCTCATGGTGTTGCAAAGCAAGTTCGTGCCGCAGCTCAGGAACGAGGCCTTGTGGTAATTGATGCCACCTGCCCACT
CGTTACTAAGGTGCATCTTGAGATTCATAGATACAATAAGAACGGGTACAAAATTATCCTAATAGGGCATAAAGGACACA
GAGAAGTTGAAGGTAGCATGGGGCAAATACAGAGTGAAGTCCTGTTAGTACAGAATGTTGATGATGTGTATAACCTACCG
GTTGATGCCGAGACGAAACTTGCATATGTAACTCAAACAACCTTAAGTGTTGATGATACCAGGGTGATAGTTGAAGCGCT
GAAGACTAGATTTCCAGATATTGTAGGACCTGACTTAAAAGACATATGTTACGCAACTCAAAATAGGCAAACTGCTGTAA
AAGAGCTTGCCTCTATGGTTGATGTAATGCTAATCATGGGTGGTAAGAACAGCTCTAACACCAACAGACTGCTGGATCTT
GCAAAAGTTCGCAATCCTCGTTCTTTTCTTATAGGATCCTACGAAGATGTAGACCTGAGTTGGTTTGCAGATACTAAGAA
AATAGGTGTCACAGCAGGGGCGTCTGCTCCGGCCATTCTGGTTGAAGAGCTTTTAAAATATTTTTCCTCTAAAATGAACA
TAAAGGTGTCGACCATGGATGGGGTACGGGAGAACATTGTCTTCAAACTACCTAATTTGAATGAAAGCTCTCACAGCTTC
TCCAGTGACGCTTAA

Upstream 100 bases:

>100_bases
CAGGTGTATACAAACTTGCGAAATTTAGTTTAGTAGTAACTCTATAAGTGGTTGGTTTCTGTAACTGCAAAGCACTGCTT
TCAGTATCTGTGGGGGTTTT

Downstream 100 bases:

>100_bases
ATGCTGTAATAGTGATATTTTCTTATTAGTAGGCAGTTCACGTCATTGTAACACACATTACGGGCACGGGCGCTGAAGAG
AGTGAAAGCTTTTGTACGTG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MHSNCNDVEVILAQPRGFCAGVERAVAILDMVVDQYIASKEVYVLHEIVHNAYVVESFRKRNVKFVASLDEVPTGAVLVF
SAHGVAKQVRAAAQERGLVVIDATCPLVTKVHLEIHRYNKNGYKIILIGHKGHREVEGSMGQIQSEVLLVQNVDDVYNLP
VDAETKLAYVTQTTLSVDDTRVIVEALKTRFPDIVGPDLKDICYATQNRQTAVKELASMVDVMLIMGGKNSSNTNRLLDL
AKVRNPRSFLIGSYEDVDLSWFADTKKIGVTAGASAPAILVEELLKYFSSKMNIKVSTMDGVRENIVFKLPNLNESSHSF
SSDA

Sequences:

>Translated_324_residues
MHSNCNDVEVILAQPRGFCAGVERAVAILDMVVDQYIASKEVYVLHEIVHNAYVVESFRKRNVKFVASLDEVPTGAVLVF
SAHGVAKQVRAAAQERGLVVIDATCPLVTKVHLEIHRYNKNGYKIILIGHKGHREVEGSMGQIQSEVLLVQNVDDVYNLP
VDAETKLAYVTQTTLSVDDTRVIVEALKTRFPDIVGPDLKDICYATQNRQTAVKELASMVDVMLIMGGKNSSNTNRLLDL
AKVRNPRSFLIGSYEDVDLSWFADTKKIGVTAGASAPAILVEELLKYFSSKMNIKVSTMDGVRENIVFKLPNLNESSHSF
SSDA
>Mature_324_residues
MHSNCNDVEVILAQPRGFCAGVERAVAILDMVVDQYIASKEVYVLHEIVHNAYVVESFRKRNVKFVASLDEVPTGAVLVF
SAHGVAKQVRAAAQERGLVVIDATCPLVTKVHLEIHRYNKNGYKIILIGHKGHREVEGSMGQIQSEVLLVQNVDDVYNLP
VDAETKLAYVTQTTLSVDDTRVIVEALKTRFPDIVGPDLKDICYATQNRQTAVKELASMVDVMLIMGGKNSSNTNRLLDL
AKVRNPRSFLIGSYEDVDLSWFADTKKIGVTAGASAPAILVEELLKYFSSKMNIKVSTMDGVRENIVFKLPNLNESSHSF
SSDA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=307, Percent_Identity=50.814332247557, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35751; Mature: 35751

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSNCNDVEVILAQPRGFCAGVERAVAILDMVVDQYIASKEVYVLHEIVHNAYVVESFRK
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RNVKFVASLDEVPTGAVLVFSAHGVAKQVRAAAQERGLVVIDATCPLVTKVHLEIHRYNK
CCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEEECC
NGYKIILIGHKGHREVEGSMGQIQSEVLLVQNVDDVYNLPVDAETKLAYVTQTTLSVDDT
CCEEEEEEECCCCCCCCCCHHHHHHHEEEEECCHHHHCCCCCCCCEEEEEEEEEECCCHH
RVIVEALKTRFPDIVGPDLKDICYATQNRQTAVKELASMVDVMLIMGGKNSSNTNRLLDL
HHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHH
AKVRNPRSFLIGSYEDVDLSWFADTKKIGVTAGASAPAILVEELLKYFSSKMNIKVSTMD
HHHCCCCCEEECCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
GVRENIVFKLPNLNESSHSFSSDA
CCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MHSNCNDVEVILAQPRGFCAGVERAVAILDMVVDQYIASKEVYVLHEIVHNAYVVESFRK
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RNVKFVASLDEVPTGAVLVFSAHGVAKQVRAAAQERGLVVIDATCPLVTKVHLEIHRYNK
CCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEEECC
NGYKIILIGHKGHREVEGSMGQIQSEVLLVQNVDDVYNLPVDAETKLAYVTQTTLSVDDT
CCEEEEEEECCCCCCCCCCHHHHHHHEEEEECCHHHHCCCCCCCCEEEEEEEEEECCCHH
RVIVEALKTRFPDIVGPDLKDICYATQNRQTAVKELASMVDVMLIMGGKNSSNTNRLLDL
HHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHH
AKVRNPRSFLIGSYEDVDLSWFADTKKIGVTAGASAPAILVEELLKYFSSKMNIKVSTMD
HHHCCCCCEEECCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
GVRENIVFKLPNLNESSHSFSSDA
CCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA