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The map label for this gene is ispA [H]

Identifier: 88607928

GI number: 88607928

Start: 312675

End: 313517

Strand: Reverse

Name: ispA [H]

Synonym: APH_0329

Alternate gene names: 88607928

Gene position: 313517-312675 (Counterclockwise)

Preceding gene: 88607032

Following gene: 88607231

Centisome position: 21.31

GC content: 43.77

Gene sequence:

>843_bases
ATGATGCGTTGCCACATAAAGGAACGCGTGAAAGACTTCTCTAATTTGGTAGGAAAAGAGCTTACAAAACTCATTGAAAC
GCAGGTCACTGGAGGGCCTGCTGCAGGCCTGGTAGACGCAATCGGCTATTGTATACTCCCTCCAGGTAAGTGTCTGCGTG
CTTTTCTTGCAAGTTCCGTTGCTCAAACCTTTGGTGTAAGCATAGAGAGAACTTTGCCCCTGTGTCTTGCAATTGAGGTG
CTTCACTCTTACTCCCTTGTACATGATGACTTACCATCTATGGATAATGCGCCTACTAGGAGGGGTAAGAATAGTTGCCA
TATAGAGCATGGAGAAGCGTTAGCACTCCTCACAGGGGACGCTTTGCTGACGTTAACGTTTGAATTATTGTCTTCCATGG
ATGAAAGTTGCACTGTTAAATGCGCTATAATCAGAGTTATTTCCGATGCCTGTGGGCACCGTGGTATGGTTGCAGGACAA
GCACTTGATATGGGAAATTTAGAACCCTCACTGGAGGCTGTAAAGCAAATTCACAAATTGAAAACAGCTCGATTATTCTC
TGCTGCATGTGAATCTGGAGCAATACTAGCTGAGGTTTCTACTGCCAACCAGCAAGCATTGGCATCCTATGGTGAGACCT
TGGGGTGCGCATTTCAAGCTAAGGACGATTTATGTGATATAAATGAAGATGATAGCTGCCTGAACATTGCTCGCATATTA
GGTAGGGAAAAGACGCTTGAATACATAGACTATTTGTTACATCAAAGCGAGGAGTATCTAGAAAGAATTGATGAAGATAC
CGCAGTGTTGAAGGAACTTGTTGCGTTTATAAGAAATTTGTGA

Upstream 100 bases:

>100_bases
GCTCTAGAATAATTTTTGCTGATATGACTAACACGTAAAAACCTGGTAATACTAACAAACTGCTGTATATTAGTTGTCTC
TTATGGGTGAGGATTATGTA

Downstream 100 bases:

>100_bases
CTTATGAAAAGAATTACATGCTGGGTGCTCAAGATTGCTGTAGTGGTTTCTGCACTATTTCTTGTACTGAGAGTGGTTTC
CGTATTTCTCACAGAGAATG

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMRCHIKERVKDFSNLVGKELTKLIETQVTGGPAAGLVDAIGYCILPPGKCLRAFLASSVAQTFGVSIERTLPLCLAIEV
LHSYSLVHDDLPSMDNAPTRRGKNSCHIEHGEALALLTGDALLTLTFELLSSMDESCTVKCAIIRVISDACGHRGMVAGQ
ALDMGNLEPSLEAVKQIHKLKTARLFSAACESGAILAEVSTANQQALASYGETLGCAFQAKDDLCDINEDDSCLNIARIL
GREKTLEYIDYLLHQSEEYLERIDEDTAVLKELVAFIRNL

Sequences:

>Translated_280_residues
MMRCHIKERVKDFSNLVGKELTKLIETQVTGGPAAGLVDAIGYCILPPGKCLRAFLASSVAQTFGVSIERTLPLCLAIEV
LHSYSLVHDDLPSMDNAPTRRGKNSCHIEHGEALALLTGDALLTLTFELLSSMDESCTVKCAIIRVISDACGHRGMVAGQ
ALDMGNLEPSLEAVKQIHKLKTARLFSAACESGAILAEVSTANQQALASYGETLGCAFQAKDDLCDINEDDSCLNIARIL
GREKTLEYIDYLLHQSEEYLERIDEDTAVLKELVAFIRNL
>Mature_280_residues
MMRCHIKERVKDFSNLVGKELTKLIETQVTGGPAAGLVDAIGYCILPPGKCLRAFLASSVAQTFGVSIERTLPLCLAIEV
LHSYSLVHDDLPSMDNAPTRRGKNSCHIEHGEALALLTGDALLTLTFELLSSMDESCTVKCAIIRVISDACGHRGMVAGQ
ALDMGNLEPSLEAVKQIHKLKTARLFSAACESGAILAEVSTANQQALASYGETLGCAFQAKDDLCDINEDDSCLNIARIL
GREKTLEYIDYLLHQSEEYLERIDEDTAVLKELVAFIRNL

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=159, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786623, Length=264, Percent_Identity=35.6060606060606, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789578, Length=234, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17505681, Length=151, Percent_Identity=33.7748344370861, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 30457; Mature: 30457

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRCHIKERVKDFSNLVGKELTKLIETQVTGGPAAGLVDAIGYCILPPGKCLRAFLASSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQTFGVSIERTLPLCLAIEVLHSYSLVHDDLPSMDNAPTRRGKNSCHIEHGEALALLTGD
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCEEEEEECC
ALLTLTFELLSSMDESCTVKCAIIRVISDACGHRGMVAGQALDMGNLEPSLEAVKQIHKL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KTARLFSAACESGAILAEVSTANQQALASYGETLGCAFQAKDDLCDINEDDSCLNIARIL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHH
GREKTLEYIDYLLHQSEEYLERIDEDTAVLKELVAFIRNL
CCHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMRCHIKERVKDFSNLVGKELTKLIETQVTGGPAAGLVDAIGYCILPPGKCLRAFLASSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQTFGVSIERTLPLCLAIEVLHSYSLVHDDLPSMDNAPTRRGKNSCHIEHGEALALLTGD
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCEEEEEECC
ALLTLTFELLSSMDESCTVKCAIIRVISDACGHRGMVAGQALDMGNLEPSLEAVKQIHKL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KTARLFSAACESGAILAEVSTANQQALASYGETLGCAFQAKDDLCDINEDDSCLNIARIL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHH
GREKTLEYIDYLLHQSEEYLERIDEDTAVLKELVAFIRNL
CCHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515931 [H]