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The map label for this gene is purN [H]

Identifier: 88607914

GI number: 88607914

Start: 227379

End: 228014

Strand: Reverse

Name: purN [H]

Synonym: APH_0230

Alternate gene names: 88607914

Gene position: 228014-227379 (Counterclockwise)

Preceding gene: 88607014

Following gene: 88607534

Centisome position: 15.5

GC content: 43.71

Gene sequence:

>636_bases
GTGAAGAAGGAACTACGCGTAGGTGTATTGATTTCTGGTAGGGGTTCTAATCTTGAAGCGTTAGCTAAGGCGTTTTCGAC
TGAAGAGTCCTCTGTTGTGATTTCATGTGTTATTTCTAATAATGCTGAAGCACGCGGGCTCTTGATCGCTCAGAGTTACG
GAATTCCTACCTTTGTAGTTAAGCGGAAACCGCTGGATATAGAGCATATCAGTACGGTACTAAGGGAGCATGATGTAGAT
CTAGTGTGCCTTGCAGGATTCATGAGCATACTGCCTGAAAAGTTTGTCACAGATTGGCATCACAAGATAATTAACATTCA
CCCTTCACTTCTCCCATCTTTTAAGGGATTAAATGCTCAGGAACAAGCCTATAAAGCAGGTGTTAAAATAGCGGGGTGCA
CCCTACATTATGTATATCAAGAGCTTGATGCCGGTCCTATAATAATGCAGGCTGCAGTGCCTGTGCTTAGAGAAGACACT
GCTGAGAGTTTAGCTAGTCGTATTCTAGCTGCTGAGCACGTATGTTATCCCAAGGGTGTGAAGCTCATTGCTCAGGATAA
AATCAAGCTTTGCGATGATGGCACTGTGCAATGTACGGGCGAGGATGAGCTCTTCTTATTTCAGGAGAACTTTTAA

Upstream 100 bases:

>100_bases
AAAAGGGAGCTACGGGTTATGGCGTGATGTTGCTGAATAGGTTTATCCAGAAATATCATGAGGGAACTGCTCATCAAAAA
CCTTCAGGGAAGTAGCTTCT

Downstream 100 bases:

>100_bases
CTACACGTTACATTTCTGTTTGTTGTGTGTATTTCCGTGTTTCTGCGGTTATTATACCTAGTAGGGTCTTGCATAAAAGG
ATGATTGCTATTGCAGCACA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKKELRVGVLISGRGSNLEALAKAFSTEESSVVISCVISNNAEARGLLIAQSYGIPTFVVKRKPLDIEHISTVLREHDVD
LVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQEQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDT
AESLASRILAAEHVCYPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF

Sequences:

>Translated_211_residues
MKKELRVGVLISGRGSNLEALAKAFSTEESSVVISCVISNNAEARGLLIAQSYGIPTFVVKRKPLDIEHISTVLREHDVD
LVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQEQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDT
AESLASRILAAEHVCYPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF
>Mature_211_residues
MKKELRVGVLISGRGSNLEALAKAFSTEESSVVISCVISNNAEARGLLIAQSYGIPTFVVKRKPLDIEHISTVLREHDVD
LVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQEQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDT
AESLASRILAAEHVCYPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=48.7562189054726, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=48.7562189054726, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=48.7562189054726, Blast_Score=184, Evalue=6e-47,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=36.5979381443299, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787483, Length=163, Percent_Identity=36.8098159509202, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=42.7807486631016, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=30.8080808080808, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=44.2708333333333, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23192; Mature: 23192

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELRVGVLISGRGSNLEALAKAFSTEESSVVISCVISNNAEARGLLIAQSYGIPTFVV
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEEE
KRKPLDIEHISTVLREHDVDLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQ
ECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCCCCHH
EQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVCYPKGV
HHHHHCCCEEECHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KLIAQDKIKLCDDGTVQCTGEDELFLFQENF
EEEECCCEEECCCCEEEECCCCCEEEEECCC
>Mature Secondary Structure
MKKELRVGVLISGRGSNLEALAKAFSTEESSVVISCVISNNAEARGLLIAQSYGIPTFVV
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEEE
KRKPLDIEHISTVLREHDVDLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQ
ECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCCCCHH
EQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVCYPKGV
HHHHHCCCEEECHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KLIAQDKIKLCDDGTVQCTGEDELFLFQENF
EEEECCCEEECCCCEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]