Click here to switch to the map view.

The map label for this gene is addA [H]

Identifier: 88607848

GI number: 88607848

Start: 256969

End: 259335

Strand: Reverse

Name: addA [H]

Synonym: APH_0258

Alternate gene names: 88607848

Gene position: 259335-256969 (Counterclockwise)

Preceding gene: 88607381

Following gene: 88607359

Centisome position: 17.63

GC content: 38.36

Gene sequence:

>2367_bases
ATGCTTCTCCCGGAAAATGAACTAATAGACGTATTGAGAGAAACTTGTTACCAAAATGCCGACGATATACTCATAAATAG
AGCACGTAACCTATTCTTTCGCATACCAAATATACTTAAAGTGCAAACTGTACACGGATTTTGCAAGTCTCTTATATCTT
CTTTTCCTTCAGAAGCAGGCATATCAGCTAATTTTGAGGTCAGGGAGCTATCAGAATCGTACCCAAAGATTTTCTACAAG
CTGCTTCATGAAAGTGACTACATAGCAGAGCACTTAAAAGAAGTAGCATGTGAGCTCAAGGAAAGTACACTATTTGATCT
TCTGTATAAAATCATGAAAAAACCTCATATAGATCTAGAAGTACCACAACCTACAGATCTACACGTAAAACCAGATCATT
TATTATGCCCAGAAGAAATAATCGATGCCCTAAACTGTGGAGGACTCAGAGATAAAAAAATCTCTACTAAGCTGCGTTCA
TGGAACAACTTAGTCCACAAAACACATAAAAAAATCGAAGAATACCTAAAAACTTTCATCTGTATTGCATCTCTAGAAAA
AAAAGACATATCTAGCATTGCAAGCAAACAAGTCCTGCAACAGTGCCCAACGGTTGGTGGAGCAATCGTCAACGAACAGG
AAAAGCTTTTAGAATTCGCAGAACAATACTACAGCGCAAGAATATCGAAACGCACGTTTCATATAATGCAACTAGTGCGC
CATGGCATTCGTATATACGAATACGATAAAAAAAATGCAAAATACCTCAACTATGATGACATAATAAATCTATCCCTAAA
GCTTATTTCTGACCCTACTCACAAAGATTTTGTCCTGTTTTCTCTCGATAGCCAAATTAATCACATACTAGTTGATGAAT
CACAAGACAATAGCCTAGAACAATGGCATATAGTCGCAGAACTCTGCAATGAGTTCTTTACTGGGTTAAGCAGCAATACG
AACCAACGCACCCTTTTTGTAGTGGGCGACGTAAAACAGTCTATATATGGGTTTCAGAATGCTCGACCAGACTATTTCCA
CTTCATGCACAAGTACTTCGCTCAAAAGAGCACAAACCCTGTAACTATACAATTGAGCGATTCATTCCGATCTACCCCAC
CAATCCTCGCCCTGGTAGATAAAGTATTCAATACACTCCGTGAACAGGTGTCATTTCAATCTCAGGAAATCGTACACAAT
ACATATCGCGCTAATGAATACGGATACGTTGAAATATGGCCACTATTGCGCTCCACTCGTCGTAAAAAACCTTATGCCTG
GGATTTAGAAAGCATAAAAGAATGTGGTGAAAATGCCGATCATTCCTTGTTACTTGCACAAACCATATCACAAACTATAT
CTTCTTGGATCAAAGAAGAAAGAAAATTACAAGCAAAAAATCGCCCCGTAGAAGCAAGTGACATACTAATACTTGTACGC
CACAGAAGCGTATTCGTCGATCAAATGATTGCAAGCCTAAAACAGGAAAATATCCCTGTTACAGGTAGAGACCGCTGCAA
CATAATGGATTACGTTGTCATCCAAGACCTGGTAAATTTAGGTGAATTCTTACTACTTCCAGAAAATGATATATCTCTCG
CAACGTTGCTGAAATCCCCAATATTTCGGTATACAGACTCCATGTTGTTTGAAATAGTAAGCAGCAGCTCTGAGAAATGT
TCTCTTTGGAACAAACTACAGAAGTTTACAACAATGCAAGAGACGGTAAAATATCTGAAACTGCTTATATCAATATCCCA
GAACCGCAGCCCATTAGATTTGTATTTTTTCATACTCTCACAACACAAAGAGCAATTTATCAAATGTCTTGGACAAACAT
CTGTTGAGATCATTGAAGAATTTATAAACCTTCTTATAGCATTTCAGTCAAATAACCTAAATCTGCTCGAGTACTTTATC
CAATGGATAAAAAGCACTAACCCCGTAACTAAAAGCGACATGAGTATCGCAAAAAATTTAGTACGAATAATGACAGTACA
TAGCGCGAAGGGTATGCAAGCTCCTATAGTGTTTTTACCAGATACAACTTCAGTGCCTAAGTGTGATCTACAGGTTGTAT
TTGATGATAAAAACACGCCATTATGGTGCGCTAGCGATACAAATTCTTATTGTAAAAACCTGAAGCTCCGCAAAAGCCAG
GAGGAATATAACGAATACCTAAGGCTTTTATACGTGGCTATGACTCGTGCAGAAGACGAACTTTACATAGCAGGCATAGG
CACAGCAAATAGTAGGTCCTGGTACGATATTATCACGAACTCCAGCGCAGAAATACTCCACAAGAAGAATACTTGTCTAT
CCCCCATGTTTCCTGAGCCAATAGACGTTTTATACCTAGAGCACTAA

Upstream 100 bases:

>100_bases
ATTACTTATGGCAGGACATGAAAACATATTATGCCTAACGTTTACTAATGCTGCTGCATATGAAATTAAGGAGAGAATCT
TCGCGATGACAGAAGGTTGG

Downstream 100 bases:

>100_bases
TCTACAGACAGTGTGCTATGGCTTAGCTCTCTACTTAAAACTCACTATTTCATGCGGATGATGCAGTGACCGGACAGCTT
TCTATTCTCTAAATCCCTGT

Product: ATP-dependent DNA helicase UvrD

Products: NA

Alternate protein names: ATP-dependent helicase/nuclease AddA [H]

Number of amino acids: Translated: 788; Mature: 788

Protein sequence:

>788_residues
MLLPENELIDVLRETCYQNADDILINRARNLFFRIPNILKVQTVHGFCKSLISSFPSEAGISANFEVRELSESYPKIFYK
LLHESDYIAEHLKEVACELKESTLFDLLYKIMKKPHIDLEVPQPTDLHVKPDHLLCPEEIIDALNCGGLRDKKISTKLRS
WNNLVHKTHKKIEEYLKTFICIASLEKKDISSIASKQVLQQCPTVGGAIVNEQEKLLEFAEQYYSARISKRTFHIMQLVR
HGIRIYEYDKKNAKYLNYDDIINLSLKLISDPTHKDFVLFSLDSQINHILVDESQDNSLEQWHIVAELCNEFFTGLSSNT
NQRTLFVVGDVKQSIYGFQNARPDYFHFMHKYFAQKSTNPVTIQLSDSFRSTPPILALVDKVFNTLREQVSFQSQEIVHN
TYRANEYGYVEIWPLLRSTRRKKPYAWDLESIKECGENADHSLLLAQTISQTISSWIKEERKLQAKNRPVEASDILILVR
HRSVFVDQMIASLKQENIPVTGRDRCNIMDYVVIQDLVNLGEFLLLPENDISLATLLKSPIFRYTDSMLFEIVSSSSEKC
SLWNKLQKFTTMQETVKYLKLLISISQNRSPLDLYFFILSQHKEQFIKCLGQTSVEIIEEFINLLIAFQSNNLNLLEYFI
QWIKSTNPVTKSDMSIAKNLVRIMTVHSAKGMQAPIVFLPDTTSVPKCDLQVVFDDKNTPLWCASDTNSYCKNLKLRKSQ
EEYNEYLRLLYVAMTRAEDELYIAGIGTANSRSWYDIITNSSAEILHKKNTCLSPMFPEPIDVLYLEH

Sequences:

>Translated_788_residues
MLLPENELIDVLRETCYQNADDILINRARNLFFRIPNILKVQTVHGFCKSLISSFPSEAGISANFEVRELSESYPKIFYK
LLHESDYIAEHLKEVACELKESTLFDLLYKIMKKPHIDLEVPQPTDLHVKPDHLLCPEEIIDALNCGGLRDKKISTKLRS
WNNLVHKTHKKIEEYLKTFICIASLEKKDISSIASKQVLQQCPTVGGAIVNEQEKLLEFAEQYYSARISKRTFHIMQLVR
HGIRIYEYDKKNAKYLNYDDIINLSLKLISDPTHKDFVLFSLDSQINHILVDESQDNSLEQWHIVAELCNEFFTGLSSNT
NQRTLFVVGDVKQSIYGFQNARPDYFHFMHKYFAQKSTNPVTIQLSDSFRSTPPILALVDKVFNTLREQVSFQSQEIVHN
TYRANEYGYVEIWPLLRSTRRKKPYAWDLESIKECGENADHSLLLAQTISQTISSWIKEERKLQAKNRPVEASDILILVR
HRSVFVDQMIASLKQENIPVTGRDRCNIMDYVVIQDLVNLGEFLLLPENDISLATLLKSPIFRYTDSMLFEIVSSSSEKC
SLWNKLQKFTTMQETVKYLKLLISISQNRSPLDLYFFILSQHKEQFIKCLGQTSVEIIEEFINLLIAFQSNNLNLLEYFI
QWIKSTNPVTKSDMSIAKNLVRIMTVHSAKGMQAPIVFLPDTTSVPKCDLQVVFDDKNTPLWCASDTNSYCKNLKLRKSQ
EEYNEYLRLLYVAMTRAEDELYIAGIGTANSRSWYDIITNSSAEILHKKNTCLSPMFPEPIDVLYLEH
>Mature_788_residues
MLLPENELIDVLRETCYQNADDILINRARNLFFRIPNILKVQTVHGFCKSLISSFPSEAGISANFEVRELSESYPKIFYK
LLHESDYIAEHLKEVACELKESTLFDLLYKIMKKPHIDLEVPQPTDLHVKPDHLLCPEEIIDALNCGGLRDKKISTKLRS
WNNLVHKTHKKIEEYLKTFICIASLEKKDISSIASKQVLQQCPTVGGAIVNEQEKLLEFAEQYYSARISKRTFHIMQLVR
HGIRIYEYDKKNAKYLNYDDIINLSLKLISDPTHKDFVLFSLDSQINHILVDESQDNSLEQWHIVAELCNEFFTGLSSNT
NQRTLFVVGDVKQSIYGFQNARPDYFHFMHKYFAQKSTNPVTIQLSDSFRSTPPILALVDKVFNTLREQVSFQSQEIVHN
TYRANEYGYVEIWPLLRSTRRKKPYAWDLESIKECGENADHSLLLAQTISQTISSWIKEERKLQAKNRPVEASDILILVR
HRSVFVDQMIASLKQENIPVTGRDRCNIMDYVVIQDLVNLGEFLLLPENDISLATLLKSPIFRYTDSMLFEIVSSSSEKC
SLWNKLQKFTTMQETVKYLKLLISISQNRSPLDLYFFILSQHKEQFIKCLGQTSVEIIEEFINLLIAFQSNNLNLLEYFI
QWIKSTNPVTKSDMSIAKNLVRIMTVHSAKGMQAPIVFLPDTTSVPKCDLQVVFDDKNTPLWCASDTNSYCKNLKLRKSQ
EEYNEYLRLLYVAMTRAEDELYIAGIGTANSRSWYDIITNSSAEILHKKNTCLSPMFPEPIDVLYLEH

Specific function: The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domai

COG id: COG1074

COG function: function code L; ATP-dependent exoDNAse (exonuclease V) beta subunit (contains helicase and exonuclease domains)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789183, Length=745, Percent_Identity=21.6107382550336, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014152
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR011604
- InterPro:   IPR014016
- InterPro:   IPR011335 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 91201; Mature: 91201

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPENELIDVLRETCYQNADDILINRARNLFFRIPNILKVQTVHGFCKSLISSFPSEAG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
ISANFEVRELSESYPKIFYKLLHESDYIAEHLKEVACELKESTLFDLLYKIMKKPHIDLE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VPQPTDLHVKPDHLLCPEEIIDALNCGGLRDKKISTKLRSWNNLVHKTHKKIEEYLKTFI
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIASLEKKDISSIASKQVLQQCPTVGGAIVNEQEKLLEFAEQYYSARISKRTFHIMQLVR
HHHHCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGIRIYEYDKKNAKYLNYDDIINLSLKLISDPTHKDFVLFSLDSQINHILVDESQDNSLE
CCCEEEEECCCCCCCCCHHHHHHHHHEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHH
QWHIVAELCNEFFTGLSSNTNQRTLFVVGDVKQSIYGFQNARPDYFHFMHKYFAQKSTNP
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
VTIQLSDSFRSTPPILALVDKVFNTLREQVSFQSQEIVHNTYRANEYGYVEIWPLLRSTR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
RKKPYAWDLESIKECGENADHSLLLAQTISQTISSWIKEERKLQAKNRPVEASDILILVR
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEH
HRSVFVDQMIASLKQENIPVTGRDRCNIMDYVVIQDLVNLGEFLLLPENDISLATLLKSP
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
IFRYTDSMLFEIVSSSSEKCSLWNKLQKFTTMQETVKYLKLLISISQNRSPLDLYFFILS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QHKEQFIKCLGQTSVEIIEEFINLLIAFQSNNLNLLEYFIQWIKSTNPVTKSDMSIAKNL
HHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
VRIMTVHSAKGMQAPIVFLPDTTSVPKCDLQVVFDDKNTPLWCASDTNSYCKNLKLRKSQ
HHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHCCCCCCCH
EEYNEYLRLLYVAMTRAEDELYIAGIGTANSRSWYDIITNSSAEILHKKNTCLSPMFPEP
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCCC
IDVLYLEH
CCEEEECC
>Mature Secondary Structure
MLLPENELIDVLRETCYQNADDILINRARNLFFRIPNILKVQTVHGFCKSLISSFPSEAG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
ISANFEVRELSESYPKIFYKLLHESDYIAEHLKEVACELKESTLFDLLYKIMKKPHIDLE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VPQPTDLHVKPDHLLCPEEIIDALNCGGLRDKKISTKLRSWNNLVHKTHKKIEEYLKTFI
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIASLEKKDISSIASKQVLQQCPTVGGAIVNEQEKLLEFAEQYYSARISKRTFHIMQLVR
HHHHCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGIRIYEYDKKNAKYLNYDDIINLSLKLISDPTHKDFVLFSLDSQINHILVDESQDNSLE
CCCEEEEECCCCCCCCCHHHHHHHHHEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHH
QWHIVAELCNEFFTGLSSNTNQRTLFVVGDVKQSIYGFQNARPDYFHFMHKYFAQKSTNP
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
VTIQLSDSFRSTPPILALVDKVFNTLREQVSFQSQEIVHNTYRANEYGYVEIWPLLRSTR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
RKKPYAWDLESIKECGENADHSLLLAQTISQTISSWIKEERKLQAKNRPVEASDILILVR
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEH
HRSVFVDQMIASLKQENIPVTGRDRCNIMDYVVIQDLVNLGEFLLLPENDISLATLLKSP
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
IFRYTDSMLFEIVSSSSEKCSLWNKLQKFTTMQETVKYLKLLISISQNRSPLDLYFFILS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QHKEQFIKCLGQTSVEIIEEFINLLIAFQSNNLNLLEYFIQWIKSTNPVTKSDMSIAKNL
HHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
VRIMTVHSAKGMQAPIVFLPDTTSVPKCDLQVVFDDKNTPLWCASDTNSYCKNLKLRKSQ
HHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHCCCCCCCH
EEYNEYLRLLYVAMTRAEDELYIAGIGTANSRSWYDIITNSSAEILHKKNTCLSPMFPEP
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCCC
IDVLYLEH
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA