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The map label for this gene is eno

Identifier: 88607824

GI number: 88607824

Start: 717708

End: 718997

Strand: Reverse

Name: eno

Synonym: APH_0695

Alternate gene names: 88607824

Gene position: 718997-717708 (Counterclockwise)

Preceding gene: 88606796

Following gene: 88606806

Centisome position: 48.87

GC content: 43.26

Gene sequence:

>1290_bases
GTGCTATATTCTAGGTCCGTTGCCAAAATAAATGGTGTCTCTGCTCGTCAGATACTTGATAGCAGGGGGCGTCCCACTGT
TGAAGCAGTTGTATCGTTATCAGATGGAGCCCTTGGAGTTGTATCTGTTCCTTCTGGTGCGTCAGTAGGGAAAAATGAGG
CCTTAGAGCTCCGCGACAAGGATATGAATAAGTACGGAGGCCACGGCGTAACAAAGGCTGTTGAGAATGTTAATTCTATA
ATAGCGCCGCGACTGGTAAACACAGACCCCTTTAACCAAAAGGCTTTAGATGATCTCTTGATAGAGCTTGATGGAACAGA
TAATAAGTCAAGGCTCGGTGCGAATGCGACGCTTGCTGTCTCCGTTGCTACAGCTAAGGCGGCAGCTGCTAGTCTTAAAC
TCCCACTCTACAGATATATAGGTGGAGTTGCCTCCAGAGAGATGCCAGTGCCATTGGTAAACGTCATAAACGGTGGCTTG
CATGCAGATAATGGGCTGGATTTTCAAGAATTTATGATCATGCCTATAGGAGCCTCTACTTTTTCCGATGCTCTTAGAAT
GTGCGCTGAGGTGTTTTTATCGCTCAAAGAAATATTGAAAAGTAATAAGCTGAGTACAAATACTGGCGATGAAGGTGGTT
TTGCACCTAATCTTGAGAGTAATGATAGGGTATTCTGTATACTGCTAGAAGCTATTGAGAAGGCTGGCTACAAACCTTCT
ATAGATTTCGCTCTGGCTCTGGATGTTGCTGCTTCTACATTCTATGATGGCAAAATTTACAAGTTTTCTGGCTCAAGCAT
GAGTAGTGCGGAGATGGTGTCGTACTATGAAGAACTAGTAAAAAAGTACCCTATAATCTCCATTGAAGACGGCATTGCTG
AAGATGATCTCGCTGGCTGGAAAGAATTGACTGTGCGCTTGGGTAAGAAAGTGCAATTAGTTGGCGATGATCTGTTTGTC
ACTAATCCTCGCTTGATTAAGGACTTTTCTGAGAGAGGGCTTGCCAATGCTGTACTTATTAAACCGAACCAGATTGGTAC
GCTGTCGGAAACTATAGAAGCTATTAGGCTTGCTTCTATGTCAAATTTTAATTCCATTGTTTCTCATAGATCTGGAGATA
CAGAAGATCCGTTTATTGCTCATATTGCTGTGGCTTTGAACTGTGGTCAAATTAAGACGGGTTCTATGTCTCGATCTGAA
CGCATGGCTAAGTACAATGAATTGTTAAGGATTGAAGAAGATCTCTGTGGTACTGCCATACTTCGTTCCGTTAAGATAGG
GCGACATTAG

Upstream 100 bases:

>100_bases
GGCCCTGCGCACAAATGCAAAACTAACGGCTGCAGGATATCTTTACTTTTTGGGGAATATTGTTTACACTGACGCCCACT
CAGTATATCGAGAGTTGCGT

Downstream 100 bases:

>100_bases
CTTTATGAGTTTCATTGACGAAGCCAAGGTTTTTGTTAAGGGCGGCAATGGCGGAAATGGCTGCGTGAGTTTCCGTAGGG
AAAAGTATATTGAGTTTGGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLYSRSVAKINGVSARQILDSRGRPTVEAVVSLSDGALGVVSVPSGASVGKNEALELRDKDMNKYGGHGVTKAVENVNSI
IAPRLVNTDPFNQKALDDLLIELDGTDNKSRLGANATLAVSVATAKAAAASLKLPLYRYIGGVASREMPVPLVNVINGGL
HADNGLDFQEFMIMPIGASTFSDALRMCAEVFLSLKEILKSNKLSTNTGDEGGFAPNLESNDRVFCILLEAIEKAGYKPS
IDFALALDVAASTFYDGKIYKFSGSSMSSAEMVSYYEELVKKYPIISIEDGIAEDDLAGWKELTVRLGKKVQLVGDDLFV
TNPRLIKDFSERGLANAVLIKPNQIGTLSETIEAIRLASMSNFNSIVSHRSGDTEDPFIAHIAVALNCGQIKTGSMSRSE
RMAKYNELLRIEEDLCGTAILRSVKIGRH

Sequences:

>Translated_429_residues
MLYSRSVAKINGVSARQILDSRGRPTVEAVVSLSDGALGVVSVPSGASVGKNEALELRDKDMNKYGGHGVTKAVENVNSI
IAPRLVNTDPFNQKALDDLLIELDGTDNKSRLGANATLAVSVATAKAAAASLKLPLYRYIGGVASREMPVPLVNVINGGL
HADNGLDFQEFMIMPIGASTFSDALRMCAEVFLSLKEILKSNKLSTNTGDEGGFAPNLESNDRVFCILLEAIEKAGYKPS
IDFALALDVAASTFYDGKIYKFSGSSMSSAEMVSYYEELVKKYPIISIEDGIAEDDLAGWKELTVRLGKKVQLVGDDLFV
TNPRLIKDFSERGLANAVLIKPNQIGTLSETIEAIRLASMSNFNSIVSHRSGDTEDPFIAHIAVALNCGQIKTGSMSRSE
RMAKYNELLRIEEDLCGTAILRSVKIGRH
>Mature_429_residues
MLYSRSVAKINGVSARQILDSRGRPTVEAVVSLSDGALGVVSVPSGASVGKNEALELRDKDMNKYGGHGVTKAVENVNSI
IAPRLVNTDPFNQKALDDLLIELDGTDNKSRLGANATLAVSVATAKAAAASLKLPLYRYIGGVASREMPVPLVNVINGGL
HADNGLDFQEFMIMPIGASTFSDALRMCAEVFLSLKEILKSNKLSTNTGDEGGFAPNLESNDRVFCILLEAIEKAGYKPS
IDFALALDVAASTFYDGKIYKFSGSSMSSAEMVSYYEELVKKYPIISIEDGIAEDDLAGWKELTVRLGKKVQLVGDDLFV
TNPRLIKDFSERGLANAVLIKPNQIGTLSETIEAIRLASMSNFNSIVSHRSGDTEDPFIAHIAVALNCGQIKTGSMSRSE
RMAKYNELLRIEEDLCGTAILRSVKIGRH

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=421, Percent_Identity=49.8812351543943, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=47.196261682243, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=47.196261682243, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI4503571, Length=421, Percent_Identity=47.7434679334917, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=42.7230046948357, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI169201331, Length=345, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI169201757, Length=345, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI239744207, Length=345, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=52.9976019184652, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71995829, Length=426, Percent_Identity=48.5915492957746, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17536383, Length=426, Percent_Identity=48.5915492957746, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32563855, Length=185, Percent_Identity=44.8648648648649, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324974, Length=423, Percent_Identity=48.2269503546099, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324969, Length=423, Percent_Identity=48.2269503546099, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323985, Length=423, Percent_Identity=48.2269503546099, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321693, Length=429, Percent_Identity=46.6200466200466, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6321968, Length=428, Percent_Identity=46.4953271028037, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24580918, Length=422, Percent_Identity=47.6303317535545, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580916, Length=422, Percent_Identity=47.6303317535545, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580920, Length=422, Percent_Identity=47.6303317535545, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580914, Length=422, Percent_Identity=47.6303317535545, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI281360527, Length=422, Percent_Identity=47.6303317535545, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI17137654, Length=422, Percent_Identity=47.6303317535545, Blast_Score=351, Evalue=5e-97,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ANAPZ (Q2GK24)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505277.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2GK24
- SMR:   Q2GK24
- STRING:   Q2GK24
- GeneID:   3930692
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0695
- NMPDR:   fig|212042.5.peg.677
- TIGR:   APH_0695
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   APHA212042:APH_0695-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46231; Mature: 46231

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 211-211 ACT_SITE 341-341 BINDING 161-161 BINDING 170-170 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSRSVAKINGVSARQILDSRGRPTVEAVVSLSDGALGVVSVPSGASVGKNEALELRDK
CCCCCCCHHHCCCHHHHHHHCCCCCCCHHEEEECCCCEEEEECCCCCCCCCCCCHHHHHC
DMNKYGGHGVTKAVENVNSIIAPRLVNTDPFNQKALDDLLIELDGTDNKSRLGANATLAV
CCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEECCCCCHHHCCCCCEEEE
SVATAKAAAASLKLPLYRYIGGVASREMPVPLVNVINGGLHADNGLDFQEFMIMPIGAST
EEHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHH
FSDALRMCAEVFLSLKEILKSNKLSTNTGDEGGFAPNLESNDRVFCILLEAIEKAGYKPS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCC
IDFALALDVAASTFYDGKIYKFSGSSMSSAEMVSYYEELVKKYPIISIEDGIAEDDLAGW
CCCEEEEHHHHHHEECCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCH
KELTVRLGKKVQLVGDDLFVTNPRLIKDFSERGLANAVLIKPNQIGTLSETIEAIRLASM
HHHHHHHCCEEEEECCCEEECCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
SNFNSIVSHRSGDTEDPFIAHIAVALNCGQIKTGSMSRSERMAKYNELLRIEEDLCGTAI
HHHHHHHHCCCCCCCCCHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRSVKIGRH
HHHHHCCCC
>Mature Secondary Structure
MLYSRSVAKINGVSARQILDSRGRPTVEAVVSLSDGALGVVSVPSGASVGKNEALELRDK
CCCCCCCHHHCCCHHHHHHHCCCCCCCHHEEEECCCCEEEEECCCCCCCCCCCCHHHHHC
DMNKYGGHGVTKAVENVNSIIAPRLVNTDPFNQKALDDLLIELDGTDNKSRLGANATLAV
CCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEECCCCCHHHCCCCCEEEE
SVATAKAAAASLKLPLYRYIGGVASREMPVPLVNVINGGLHADNGLDFQEFMIMPIGAST
EEHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHH
FSDALRMCAEVFLSLKEILKSNKLSTNTGDEGGFAPNLESNDRVFCILLEAIEKAGYKPS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCC
IDFALALDVAASTFYDGKIYKFSGSSMSSAEMVSYYEELVKKYPIISIEDGIAEDDLAGW
CCCEEEEHHHHHHEECCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCH
KELTVRLGKKVQLVGDDLFVTNPRLIKDFSERGLANAVLIKPNQIGTLSETIEAIRLASM
HHHHHHHCCEEEEECCCEEECCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
SNFNSIVSHRSGDTEDPFIAHIAVALNCGQIKTGSMSRSERMAKYNELLRIEEDLCGTAI
HHHHHHHHCCCCCCCCCHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRSVKIGRH
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA