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The map label for this gene is bioB

Identifier: 88607749

GI number: 88607749

Start: 216504

End: 217472

Strand: Reverse

Name: bioB

Synonym: APH_0218

Alternate gene names: 88607749

Gene position: 217472-216504 (Counterclockwise)

Preceding gene: 88606768

Following gene: 88607504

Centisome position: 14.78

GC content: 45.2

Gene sequence:

>969_bases
GTGATAAGGAATAATTGGACTCTTGAAGAAACGTTAGAGCTCTTTGGCCTACCGTTTAATGATTTGCTATTCAAGGCTCA
TATGGTGCATAGGGAGTACTTTCGTCACAACGAGGTACAGGTTTGTGCACTGGCAAATATTAAAGCGGGTGGTTGTCCGG
AGAACTGTTGTTATTGTTCCCAGAGCGCACATAACAAAACTGGCCTTACCAAGAAGGGGCTATCAAGTGTTGAGGAAGTG
CGTACTGCGGCGCGGCGTGCCAAAGATGTTGGGGTACACAGGTTTTGTTTTGCTGCAGCATGGCGTAACCTGCATGATCG
GGATGTTGACCGTATCTGCGAGTTTGTCAAAGCTATAAAAGAAGAGGGTCTGGAGAGCTGTGCATCTCTAGGCATGTTGA
AGCTTGAACAGGCGCAGAAGCTCAAGGAAGCTGGTTTAGACTTTTATAATCATAATGTTGATACCTCGCGGGCTTACTAT
CATAACATAGTTACCACACGAACGTACGATGAGCGGCTAGACACGATCAATAATGTGCAAGAGGCTGGGATTAATGTATG
CTGTGGTGGCATATTAGGAATGGGTGAGAGCACAGAGGATCGGGCTCAGATGCTATTAACCCTTGCAAACCTTAAACAGC
ATCCACGTTCTGTACCAATCAACAGACTTGTGGCATTCAAAGGTACTCCTTTAGAGGGTGCAAAGAAAGTCAATAGCGTT
GATTTTGTTCGTACCATAGCGGTTGCTCGTATAATGATGCCAGCATCATATGTACGTCTTGCTGCTGGTAGGTCAGACAT
GACGGAAGAGATGCATGCTTTATGCTTTTTTGCAGGTGCAAACTCTATCTTTTACGGTGAACAGCTACTCACTACTGAAA
ATGCTACACCGGAATTTGATCAAAATTTGCTTTCTAGGCTTGGTGTTGTAGCAACACCTTCCGGAGCACATGAGTCTTGT
GCTTGTTAG

Upstream 100 bases:

>100_bases
TCTCTGAAGGAAATGGGCTTTGTGTGCAGAGAGGAGTTTGATGCCCTAGACGAGTGCTTTAGGCGCTTTCAGGCTTCCAA
GGAGAAACAAAGTCATCAGC

Downstream 100 bases:

>100_bases
GAATTCTCCATCATGTTGGTGACACAATCCTTCAAGTTGTTATACTACTGTATGACGTGGTTAGTTTACTGGTATGGCTC
TGCGATCAGCTACTGTGCGG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MIRNNWTLEETLELFGLPFNDLLFKAHMVHREYFRHNEVQVCALANIKAGGCPENCCYCSQSAHNKTGLTKKGLSSVEEV
RTAARRAKDVGVHRFCFAAAWRNLHDRDVDRICEFVKAIKEEGLESCASLGMLKLEQAQKLKEAGLDFYNHNVDTSRAYY
HNIVTTRTYDERLDTINNVQEAGINVCCGGILGMGESTEDRAQMLLTLANLKQHPRSVPINRLVAFKGTPLEGAKKVNSV
DFVRTIAVARIMMPASYVRLAAGRSDMTEEMHALCFFAGANSIFYGEQLLTTENATPEFDQNLLSRLGVVATPSGAHESC
AC

Sequences:

>Translated_322_residues
MIRNNWTLEETLELFGLPFNDLLFKAHMVHREYFRHNEVQVCALANIKAGGCPENCCYCSQSAHNKTGLTKKGLSSVEEV
RTAARRAKDVGVHRFCFAAAWRNLHDRDVDRICEFVKAIKEEGLESCASLGMLKLEQAQKLKEAGLDFYNHNVDTSRAYY
HNIVTTRTYDERLDTINNVQEAGINVCCGGILGMGESTEDRAQMLLTLANLKQHPRSVPINRLVAFKGTPLEGAKKVNSV
DFVRTIAVARIMMPASYVRLAAGRSDMTEEMHALCFFAGANSIFYGEQLLTTENATPEFDQNLLSRLGVVATPSGAHESC
AC
>Mature_322_residues
MIRNNWTLEETLELFGLPFNDLLFKAHMVHREYFRHNEVQVCALANIKAGGCPENCCYCSQSAHNKTGLTKKGLSSVEEV
RTAARRAKDVGVHRFCFAAAWRNLHDRDVDRICEFVKAIKEEGLESCASLGMLKLEQAQKLKEAGLDFYNHNVDTSRAYY
HNIVTTRTYDERLDTINNVQEAGINVCCGGILGMGESTEDRAQMLLTLANLKQHPRSVPINRLVAFKGTPLEGAKKVNSV
DFVRTIAVARIMMPASYVRLAAGRSDMTEEMHALCFFAGANSIFYGEQLLTTENATPEFDQNLLSRLGVVATPSGAHESC
AC

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=55.700325732899, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=46.8152866242038, Blast_Score=305, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ANAPZ (Q2GLB4)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504837.1
- ProteinModelPortal:   Q2GLB4
- SMR:   Q2GLB4
- STRING:   Q2GLB4
- GeneID:   3930883
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0218
- NMPDR:   fig|212042.5.peg.207
- TIGR:   APH_0218
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q2GLB4
- ProtClustDB:   CLSK2391489
- BioCyc:   APHA212042:APH_0218-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35830; Mature: 35830

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRNNWTLEETLELFGLPFNDLLFKAHMVHREYFRHNEVQVCALANIKAGGCPENCCYCS
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHEECC
QSAHNKTGLTKKGLSSVEEVRTAARRAKDVGVHRFCFAAAWRNLHDRDVDRICEFVKAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EEGLESCASLGMLKLEQAQKLKEAGLDFYNHNVDTSRAYYHNIVTTRTYDERLDTINNVQ
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
EAGINVCCGGILGMGESTEDRAQMLLTLANLKQHPRSVPINRLVAFKGTPLEGAKKVNSV
HCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHH
DFVRTIAVARIMMPASYVRLAAGRSDMTEEMHALCFFAGANSIFYGEQLLTTENATPEFD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCHHEECCCCCCHHH
QNLLSRLGVVATPSGAHESCAC
HHHHHHCCEEECCCCCCCCCCC
>Mature Secondary Structure
MIRNNWTLEETLELFGLPFNDLLFKAHMVHREYFRHNEVQVCALANIKAGGCPENCCYCS
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHEECC
QSAHNKTGLTKKGLSSVEEVRTAARRAKDVGVHRFCFAAAWRNLHDRDVDRICEFVKAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EEGLESCASLGMLKLEQAQKLKEAGLDFYNHNVDTSRAYYHNIVTTRTYDERLDTINNVQ
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
EAGINVCCGGILGMGESTEDRAQMLLTLANLKQHPRSVPINRLVAFKGTPLEGAKKVNSV
HCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHH
DFVRTIAVARIMMPASYVRLAAGRSDMTEEMHALCFFAGANSIFYGEQLLTTENATPEFD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCHHEECCCCCCHHH
QNLLSRLGVVATPSGAHESCAC
HHHHHHCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA