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The map label for this gene is pyrB

Identifier: 88607329

GI number: 88607329

Start: 529354

End: 530256

Strand: Reverse

Name: pyrB

Synonym: APH_0503

Alternate gene names: 88607329

Gene position: 530256-529354 (Counterclockwise)

Preceding gene: 88606902

Following gene: 88607493

Centisome position: 36.04

GC content: 40.31

Gene sequence:

>903_bases
ATGAAAAAGTTAAATAAACTTCTTCGAATTCAAAGCTTAAGTGATGTAGACATAGAAAATATAGTAGCGCTGGCCCATAG
ATACTTGCAGGATAGTAGCGCTAACAACGTGCTGCAGGGCAGAGTTATAGCCAATTTATTTTTCGAGAACTCCACAAGAA
CTCTACTGGCATTCGAAATTGCAGAAAAATCCCTAGGAGCTGTTTCTGTTACGCTCAATGTGGAAACTTCATCCATGAAA
AAAGGTGAAAGTGTTTCAGATACTCTCAGTACTTTGAGTGCTATGGGTGTCGACTTAGTTGTAGTACGATCAGGGTTCAG
TGGTTTCATAGATGAAGTAGTAGAAAGAGTGGGAGACTGTTGTGTTATAAATGCCGGAGATGGAAATCACGAGCACCCAA
CTCAAGCACTTACTGATTACGCTACCATACGCTACTTAAAGGGTAAGTACATTGAGGGGTTAAACGTTACTATTTGCGGT
GATATTTTTCATAGTAGGGTTGCTAGATCTAATATGAGACTACTTTCTAGGTATGGTGCTAATATAAATGTCGTTACGCC
ACCGTGCTGGTCTGCCTCTGTGCCATCAAGTAACGTGACAAAAATAACACATAGTCTTGAGGAAGGAATCAAAGATGCAG
ATGTTATCATGCTGTTGAGGATTCAAAAGGAGCGTATGTCAGGAGCCTCGTTTATATCTGCAAATGAGTACTCTCGGCAC
TATATGCTAGACAATGCGAAGCTATCGATAGCAAAAGATGATGTGATAATAATGCATCCAGGACCTATGAATCGTGGGAT
AGAGATTTCAGATGAAGTAGCTGATGCAAGCTCGTCTATTTTACTGCAAGTAAAGATGGGGGTTGCGGTAAGAAAAGCAA
TTTTACACTATATGTTGGGATGA

Upstream 100 bases:

>100_bases
TCTCCGTAATTCAATGCTCTACAAAACATGTGTAGATAATGTGCCTTTTTATACCTTTAAAATAGTGCTAAGTTTGTGAA
ACGTTGTTGCGTACAAGGGT

Downstream 100 bases:

>100_bases
GATATAAAGCAGTTGTAGAATACGAGGGCACTTCCTTTATAGGCTGGCAGAGGCAACACGGAGTGGTAGGAAAGTCAGTT
CAGGAATCTATAGAGCGCTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKKLNKLLRIQSLSDVDIENIVALAHRYLQDSSANNVLQGRVIANLFFENSTRTLLAFEIAEKSLGAVSVTLNVETSSMK
KGESVSDTLSTLSAMGVDLVVVRSGFSGFIDEVVERVGDCCVINAGDGNHEHPTQALTDYATIRYLKGKYIEGLNVTICG
DIFHSRVARSNMRLLSRYGANINVVTPPCWSASVPSSNVTKITHSLEEGIKDADVIMLLRIQKERMSGASFISANEYSRH
YMLDNAKLSIAKDDVIIMHPGPMNRGIEISDEVADASSSILLQVKMGVAVRKAILHYMLG

Sequences:

>Translated_300_residues
MKKLNKLLRIQSLSDVDIENIVALAHRYLQDSSANNVLQGRVIANLFFENSTRTLLAFEIAEKSLGAVSVTLNVETSSMK
KGESVSDTLSTLSAMGVDLVVVRSGFSGFIDEVVERVGDCCVINAGDGNHEHPTQALTDYATIRYLKGKYIEGLNVTICG
DIFHSRVARSNMRLLSRYGANINVVTPPCWSASVPSSNVTKITHSLEEGIKDADVIMLLRIQKERMSGASFISANEYSRH
YMLDNAKLSIAKDDVIIMHPGPMNRGIEISDEVADASSSILLQVKMGVAVRKAILHYMLG
>Mature_300_residues
MKKLNKLLRIQSLSDVDIENIVALAHRYLQDSSANNVLQGRVIANLFFENSTRTLLAFEIAEKSLGAVSVTLNVETSSMK
KGESVSDTLSTLSAMGVDLVVVRSGFSGFIDEVVERVGDCCVINAGDGNHEHPTQALTDYATIRYLKGKYIEGLNVTICG
DIFHSRVARSNMRLLSRYGANINVVTPPCWSASVPSSNVTKITHSLEEGIKDADVIMLLRIQKERMSGASFISANEYSRH
YMLDNAKLSIAKDDVIIMHPGPMNRGIEISDEVADASSSILLQVKMGVAVRKAILHYMLG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=35.4632587859425, Blast_Score=151, Evalue=8e-37,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=30.5921052631579, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790703, Length=316, Percent_Identity=23.4177215189873, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=35.9375, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=33.5423197492163, Blast_Score=139, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24642586, Length=299, Percent_Identity=33.7792642140468, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ANAPZ (Q2GKK3)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505098.1
- ProteinModelPortal:   Q2GKK3
- SMR:   Q2GKK3
- STRING:   Q2GKK3
- GeneID:   3930392
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0503
- NMPDR:   fig|212042.5.peg.497
- TIGR:   APH_0503
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   APHA212042:APH_0503-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 32821; Mature: 32821

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNKLLRIQSLSDVDIENIVALAHRYLQDSSANNVLQGRVIANLFFENSTRTLLAFEI
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHEEEEEEECCCCCEEEEEEE
AEKSLGAVSVTLNVETSSMKKGESVSDTLSTLSAMGVDLVVVRSGFSGFIDEVVERVGDC
HHCCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCE
CVINAGDGNHEHPTQALTDYATIRYLKGKYIEGLNVTICGDIFHSRVARSNMRLLSRYGA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHCCC
NINVVTPPCWSASVPSSNVTKITHSLEEGIKDADVIMLLRIQKERMSGASFISANEYSRH
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCEEECCCCCCE
YMLDNAKLSIAKDDVIIMHPGPMNRGIEISDEVADASSSILLQVKMGVAVRKAILHYMLG
EEECCCEEEEECCCEEEECCCCCCCCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLNKLLRIQSLSDVDIENIVALAHRYLQDSSANNVLQGRVIANLFFENSTRTLLAFEI
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHEEEEEEECCCCCEEEEEEE
AEKSLGAVSVTLNVETSSMKKGESVSDTLSTLSAMGVDLVVVRSGFSGFIDEVVERVGDC
HHCCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCE
CVINAGDGNHEHPTQALTDYATIRYLKGKYIEGLNVTICGDIFHSRVARSNMRLLSRYGA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHCCC
NINVVTPPCWSASVPSSNVTKITHSLEEGIKDADVIMLLRIQKERMSGASFISANEYSRH
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCEEECCCCCCE
YMLDNAKLSIAKDDVIIMHPGPMNRGIEISDEVADASSSILLQVKMGVAVRKAILHYMLG
EEECCCEEEEECCCEEEECCCCCCCCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA