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The map label for this gene is rrmJ

Identifier: 88606902

GI number: 88606902

Start: 530405

End: 531148

Strand: Reverse

Name: rrmJ

Synonym: APH_0504

Alternate gene names: 88606902

Gene position: 531148-530405 (Counterclockwise)

Preceding gene: 88607141

Following gene: 88607329

Centisome position: 36.1

GC content: 41.26

Gene sequence:

>744_bases
ATGAGGAGTTCAAATCTCACAACTACAGTACATTTTCTATCACCTGTTTTCGCAGCTTTTTTTCCTTCACATGGTACAGA
GCAAGAAGATGCCCCTATAGTAAGGGTAAAGACGGCAAAGGGAAGGAAGATATCTTCTACGAATTGGATACGTAGACAGG
TAAATGACCAGTACGTATCACTTGCCAAGAAAGAGGGGTATAGATCAAGATCAGCGTATAAACTTATAGAGATTAATGAT
AAGTTTAAGATCCTGCAACGCGGGAGGTTTGTCCTGGATTTAGGTTCTAGCCCTGGAGGATGGGCTCAAGTTGCCTCTAA
GAATACTGCTATTATCGACAGCACCGAACCAACCGTTGTGGCGGTAGACATTCAGAGTATGAAAGACATACACAATGTGT
CATTTGTGCAATGTGACATTGATAGTGATCACGACCTATTGAATGAAAAATTAAGCGGAAGAAAGTTCGACGTGGTACTG
TCGGATATGGCTCCGAAGTCCTGCGGTCACAGGCAGGTTGATCACGCTAACATAATAAACTTATGTGAACTTGCAAGAGA
TATAGCTTTGGAATACTTGAATCCTAATGGAAGTTTTGTGACAAAGCTGCTACACGGTGAATATGAGCAAGAGTTTAGGC
GTTCTATAATGACGCATTTTGGTGTAGTTTCTTACTTCAAGCCCAAGTCGAGTAGGAAAGATTCCTCAGAAATCTATCTA
GTAGCTCTGAAGTTTAAAGGATAG

Upstream 100 bases:

>100_bases
TCATATAATATTTAGGGGTGCTATTTCTTTCTGGCAAATCTTTAGCAATTATTGGGGTATTGGGCGGCAATATTAAGAAC
TGCCTGTGAATTTTGGAGCC

Downstream 100 bases:

>100_bases
TGCATGCTAGTAGCTGAGGTATTCTGCAGAACTACAGCGCATCGTGTTTCTCCGTAATTCAATGCTCTACAAAACATGTG
TAGATAATGTGCCTTTTTAT

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRSSNLTTTVHFLSPVFAAFFPSHGTEQEDAPIVRVKTAKGRKISSTNWIRRQVNDQYVSLAKKEGYRSRSAYKLIEIND
KFKILQRGRFVLDLGSSPGGWAQVASKNTAIIDSTEPTVVAVDIQSMKDIHNVSFVQCDIDSDHDLLNEKLSGRKFDVVL
SDMAPKSCGHRQVDHANIINLCELARDIALEYLNPNGSFVTKLLHGEYEQEFRRSIMTHFGVVSYFKPKSSRKDSSEIYL
VALKFKG

Sequences:

>Translated_247_residues
MRSSNLTTTVHFLSPVFAAFFPSHGTEQEDAPIVRVKTAKGRKISSTNWIRRQVNDQYVSLAKKEGYRSRSAYKLIEIND
KFKILQRGRFVLDLGSSPGGWAQVASKNTAIIDSTEPTVVAVDIQSMKDIHNVSFVQCDIDSDHDLLNEKLSGRKFDVVL
SDMAPKSCGHRQVDHANIINLCELARDIALEYLNPNGSFVTKLLHGEYEQEFRRSIMTHFGVVSYFKPKSSRKDSSEIYL
VALKFKG
>Mature_247_residues
MRSSNLTTTVHFLSPVFAAFFPSHGTEQEDAPIVRVKTAKGRKISSTNWIRRQVNDQYVSLAKKEGYRSRSAYKLIEIND
KFKILQRGRFVLDLGSSPGGWAQVASKNTAIIDSTEPTVVAVDIQSMKDIHNVSFVQCDIDSDHDLLNEKLSGRKFDVVL
SDMAPKSCGHRQVDHANIINLCELARDIALEYLNPNGSFVTKLLHGEYEQEFRRSIMTHFGVVSYFKPKSSRKDSSEIYL
VALKFKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=36.405529953917, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI194097365, Length=192, Percent_Identity=36.9791666666667, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI29029591, Length=189, Percent_Identity=34.3915343915344, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI29029589, Length=189, Percent_Identity=34.3915343915344, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI7110661, Length=189, Percent_Identity=34.3915343915344, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1789569, Length=207, Percent_Identity=38.1642512077295, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553474, Length=205, Percent_Identity=35.609756097561, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17554650, Length=197, Percent_Identity=35.0253807106599, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=206, Percent_Identity=34.4660194174757, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=206, Percent_Identity=34.4660194174757, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17553860, Length=206, Percent_Identity=34.4660194174757, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319796, Length=197, Percent_Identity=36.0406091370558, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=33.8383838383838, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321302, Length=108, Percent_Identity=36.1111111111111, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=37.1559633027523, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=32.3232323232323, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=34.5744680851064, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=30.8080808080808, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ANAPZ (Q2GKK2)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505099.1
- ProteinModelPortal:   Q2GKK2
- SMR:   Q2GKK2
- STRING:   Q2GKK2
- GeneID:   3930316
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0504
- NMPDR:   fig|212042.5.peg.498
- TIGR:   APH_0504
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q2GKK2
- BioCyc:   APHA212042:APH_0504-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 27859; Mature: 27859

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: ACT_SITE 202-202 BINDING 99-99 BINDING 101-101 BINDING 123-123 BINDING 139-139 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSNLTTTVHFLSPVFAAFFPSHGTEQEDAPIVRVKTAKGRKISSTNWIRRQVNDQYVS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHH
LAKKEGYRSRSAYKLIEINDKFKILQRGRFVLDLGSSPGGWAQVASKNTAIIDSTEPTVV
HHHHCCCCCCCCEEEEEECCHHHHHHCCCEEEEECCCCCCHHHHCCCCEEEEECCCCEEE
AVDIQSMKDIHNVSFVQCDIDSDHDLLNEKLSGRKFDVVLSDMAPKSCGHRQVDHANIIN
EEEHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHH
LCELARDIALEYLNPNGSFVTKLLHGEYEQEFRRSIMTHFGVVSYFKPKSSRKDSSEIYL
HHHHHHHHHHEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEE
VALKFKG
EEEEECC
>Mature Secondary Structure
MRSSNLTTTVHFLSPVFAAFFPSHGTEQEDAPIVRVKTAKGRKISSTNWIRRQVNDQYVS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHH
LAKKEGYRSRSAYKLIEINDKFKILQRGRFVLDLGSSPGGWAQVASKNTAIIDSTEPTVV
HHHHCCCCCCCCEEEEEECCHHHHHHCCCEEEEECCCCCCHHHHCCCCEEEEECCCCEEE
AVDIQSMKDIHNVSFVQCDIDSDHDLLNEKLSGRKFDVVLSDMAPKSCGHRQVDHANIIN
EEEHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHH
LCELARDIALEYLNPNGSFVTKLLHGEYEQEFRRSIMTHFGVVSYFKPKSSRKDSSEIYL
HHHHHHHHHHEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEE
VALKFKG
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA