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The map label for this gene is ispG [H]

Identifier: 88607294

GI number: 88607294

Start: 460691

End: 461893

Strand: Direct

Name: ispG [H]

Synonym: APH_0442

Alternate gene names: 88607294

Gene position: 460691-461893 (Clockwise)

Preceding gene: 88607378

Following gene: 88607734

Centisome position: 31.31

GC content: 43.89

Gene sequence:

>1203_bases
ATGAGAGTAAGTCAAGCTGTAAGGGTTGGCAAGGTAACAATTGGTGGTGGGCATCCGGTAGTGGTGCAATCTATGGCTCT
TGGGGGTTCCGGAAGTTTTGATCGGGACATGCAGGAAGTGCTAGCCCTTGCGAGAGAGGGGTCTGAGTTAGTTAGGGTGG
CTATAAATTCTGATGAGTCTATAAAAGTAATTCCTGACATGGTTGATGTGCTGGTAAAGCATGGGTTCGATGAGAAGATG
ATCGTTGGATGCGGTCAATATGAAGTTGCTAGGCTTCTTAGAGATTATCCAACGTGTGCTGCTGCATTGGGGAAAATAAG
AATCAATCCAGGTAATATAGGTTTTGGTGATAAGAGGGATAAGAACTTTGAGGAGGTTATAGAATTCGCTATAAAGAACG
ATATTCCGGTGAGAATAGGGGTAAACTGGGGTAGTTTGGACAAGGTTCTCGTTGGGAAGCTCATGGACGATAATGCGAAT
CTTCCGGTGCCAGAATCCGCGGATGTTGTTAAGGCAAAGGCTTTGGTGCTTTCTGCTATCACGAGTGCGAAGAAAGCAGA
GGGGATAGGTTTGCCTTCTAATAGAATAGTACTTTCGTGTAAAGTCAGCAAAGTGAGAGATTTAGTGGCGGTATACACCG
CTTTAGCTAGGCAAAGTAATCATGCATTGCATCTTGGGTTAACTGAAGCGGGAGCTGGGTTAAAAGGGGTTGTTAGCAGT
GTTGCGGGTATAGCTCATCTATTGATTATGGGTATAGGTGATACTGTGAGGGTATCGCTTACTTCTCTTACTAGAGAGGG
TAGAACTGAGGAAGTAAAGGTATGTAAGGAGATTTTGCAATCATTAGGATTAAGATATTTTACAGCGCAGAGTACTTCCT
GTCCGGGGTGTAATCGTACAAATTTTGATGTTTTTCAGAAGCTTGTTTCCGGGGTGAACAAGTATATGGAAAGTAACATG
GAGGCTTGGAAGAAGAAGTATCCGGGTGTTGTGAATATGACAGTTGCGGTTATGGGATGTGTTGTTAATGGGCCTGGCGA
GAGCAAACATGCCAATCTAGGGCTTAGTATGCCTGGTAAGGGAGAGCGAGAAGTGGCTGCGGTTTACGAGGATGGCAGCA
AGTTATGTACCATTCAAGGTGCTGACATATTGGAGCAGTTTTTGAATATTATTGAGTCTTACATTGCAAAGAATTATAGC
TAA

Upstream 100 bases:

>100_bases
CAGTTTTTAGTGTAGAAATGCTATCTATTAATTGGAACTTCTCGCAGTTTTTGAGTATCTGTGACGGGGTGGTTTGTTTT
AAAGCGTAAAAATTAAGGGT

Downstream 100 bases:

>100_bases
CTGCATTGGGAAACAGTGAAAAATTACTGTGTGCGAGTTTTTTGGAGTTAGGCTTGTTAGATAGTGGGGAGAGAGTTATT
GCTTGAATCATGTGATGAGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRVSQAVRVGKVTIGGGHPVVVQSMALGGSGSFDRDMQEVLALAREGSELVRVAINSDESIKVIPDMVDVLVKHGFDEKM
IVGCGQYEVARLLRDYPTCAAALGKIRINPGNIGFGDKRDKNFEEVIEFAIKNDIPVRIGVNWGSLDKVLVGKLMDDNAN
LPVPESADVVKAKALVLSAITSAKKAEGIGLPSNRIVLSCKVSKVRDLVAVYTALARQSNHALHLGLTEAGAGLKGVVSS
VAGIAHLLIMGIGDTVRVSLTSLTREGRTEEVKVCKEILQSLGLRYFTAQSTSCPGCNRTNFDVFQKLVSGVNKYMESNM
EAWKKKYPGVVNMTVAVMGCVVNGPGESKHANLGLSMPGKGEREVAAVYEDGSKLCTIQGADILEQFLNIIESYIAKNYS

Sequences:

>Translated_400_residues
MRVSQAVRVGKVTIGGGHPVVVQSMALGGSGSFDRDMQEVLALAREGSELVRVAINSDESIKVIPDMVDVLVKHGFDEKM
IVGCGQYEVARLLRDYPTCAAALGKIRINPGNIGFGDKRDKNFEEVIEFAIKNDIPVRIGVNWGSLDKVLVGKLMDDNAN
LPVPESADVVKAKALVLSAITSAKKAEGIGLPSNRIVLSCKVSKVRDLVAVYTALARQSNHALHLGLTEAGAGLKGVVSS
VAGIAHLLIMGIGDTVRVSLTSLTREGRTEEVKVCKEILQSLGLRYFTAQSTSCPGCNRTNFDVFQKLVSGVNKYMESNM
EAWKKKYPGVVNMTVAVMGCVVNGPGESKHANLGLSMPGKGEREVAAVYEDGSKLCTIQGADILEQFLNIIESYIAKNYS
>Mature_400_residues
MRVSQAVRVGKVTIGGGHPVVVQSMALGGSGSFDRDMQEVLALAREGSELVRVAINSDESIKVIPDMVDVLVKHGFDEKM
IVGCGQYEVARLLRDYPTCAAALGKIRINPGNIGFGDKRDKNFEEVIEFAIKNDIPVRIGVNWGSLDKVLVGKLMDDNAN
LPVPESADVVKAKALVLSAITSAKKAEGIGLPSNRIVLSCKVSKVRDLVAVYTALARQSNHALHLGLTEAGAGLKGVVSS
VAGIAHLLIMGIGDTVRVSLTSLTREGRTEEVKVCKEILQSLGLRYFTAQSTSCPGCNRTNFDVFQKLVSGVNKYMESNM
EAWKKKYPGVVNMTVAVMGCVVNGPGESKHANLGLSMPGKGEREVAAVYEDGSKLCTIQGADILEQFLNIIESYIAKNYS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=391, Percent_Identity=33.2480818414322, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 42866; Mature: 42866

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSQAVRVGKVTIGGGHPVVVQSMALGGSGSFDRDMQEVLALAREGSELVRVAINSDES
CCCHHHEEECEEEECCCCCHHHHEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCC
IKVIPDMVDVLVKHGFDEKMIVGCGQYEVARLLRDYPTCAAALGKIRINPGNIGFGDKRD
EEECHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCHHHHHHCCEEECCCCCCCCCCCC
KNFEEVIEFAIKNDIPVRIGVNWGSLDKVLVGKLMDDNANLPVPESADVVKAKALVLSAI
CCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
TSAKKAEGIGLPSNRIVLSCKVSKVRDLVAVYTALARQSNHALHLGLTEAGAGLKGVVSS
HHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHH
VAGIAHLLIMGIGDTVRVSLTSLTREGRTEEVKVCKEILQSLGLRYFTAQSTSCPGCNRT
HHHHHHHHHHCCCCHHEEEHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
NFDVFQKLVSGVNKYMESNMEAWKKKYPGVVNMTVAVMGCVVNGPGESKHANLGLSMPGK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GEREVAAVYEDGSKLCTIQGADILEQFLNIIESYIAKNYS
CCCEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVSQAVRVGKVTIGGGHPVVVQSMALGGSGSFDRDMQEVLALAREGSELVRVAINSDES
CCCHHHEEECEEEECCCCCHHHHEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCC
IKVIPDMVDVLVKHGFDEKMIVGCGQYEVARLLRDYPTCAAALGKIRINPGNIGFGDKRD
EEECHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCHHHHHHCCEEECCCCCCCCCCCC
KNFEEVIEFAIKNDIPVRIGVNWGSLDKVLVGKLMDDNANLPVPESADVVKAKALVLSAI
CCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
TSAKKAEGIGLPSNRIVLSCKVSKVRDLVAVYTALARQSNHALHLGLTEAGAGLKGVVSS
HHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHH
VAGIAHLLIMGIGDTVRVSLTSLTREGRTEEVKVCKEILQSLGLRYFTAQSTSCPGCNRT
HHHHHHHHHHCCCCHHEEEHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
NFDVFQKLVSGVNKYMESNMEAWKKKYPGVVNMTVAVMGCVVNGPGESKHANLGLSMPGK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GEREVAAVYEDGSKLCTIQGADILEQFLNIIESYIAKNYS
CCCEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA