Click here to switch to the map view.

The map label for this gene is thiG [H]

Identifier: 88607265

GI number: 88607265

Start: 77472

End: 78257

Strand: Direct

Name: thiG [H]

Synonym: APH_0070

Alternate gene names: 88607265

Gene position: 77472-78257 (Clockwise)

Preceding gene: 88607118

Following gene: 88607805

Centisome position: 5.27

GC content: 44.02

Gene sequence:

>786_bases
GTGTGGGATGTATATGGTGTACAACTAAGGAGTAGATTGCTTATGGGGTCTGCGCAATTTGCGTCTCCAGAGATTTTACA
AAGATCCTTGCATAATTCAGGTGCAGAAGTAGTAACTGTGTCATTATCGAGACAGATGCCAGAAATCGGCGGTGGGCAGG
ATTATTGGGACATAATTAAGCAGAGTAAGTGCAGAGTTTTACCTAATACTGCCGGTTGTTACACGGTCAAGGATGCTGTA
CTCATGGCACAAATGGCGAGAGAAATTTTTAATACTACTTGGATAAAACTCGAAATTATTGGGGATGACTATACCCTACA
CCCTGATGTTGTGGCATTGTGTGAGGCTGCTAAGGAGCTTATTACCCAGGGTTTTGATGTTTTCCCCTATTGCACGGATG
ATCTCACAGTATGCAGACGCCTGATTGATTGTGGGTGTCGCGTAATTATGCCGGGAATATCTCCCATAGGTTCGGGTTTG
GGAGTGCTGAATATGCATGGGTTACGATTATTGCGTAACAGGTATAAGGATATAGTTCTTATTGTAGATGCTGGAATAGG
AAGGCCTTCAGATGCGTCTGTAGTGATGGAACAGGGGATCGATGGTGTGTTACTCAATAGTGCAGTAGCAAGAGCCATTG
ATCCATCAAAAATGGCATCTGCATTCAGATATGCGATAGAATGCGGTAGGCTTGGTTATGAAGCTGGGATCATCAACAAG
CGGGATCTAGCAGTAGCCAGTACAAATTTGGTTGATACGCCCTTTTGGCACCGGAATAAATCATAG

Upstream 100 bases:

>100_bases
CTATAAAGAACATAAGCTGAAGAAAGGCGATGTTATAGATATAGTGTATCCTATGCAAGGTGGATGATGTAGCGAAGTTT
TGTATTGTAGGAGCATTACT

Downstream 100 bases:

>100_bases
AGCTTTGCTACTTGCAGTGAGAGAGCGAGGCGCTTGTCTTATGCAGGGTCTGTTCGAAACCACAGGGGCAAATCTTCAGG
TATAGGTTGTGCGCATATAC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MWDVYGVQLRSRLLMGSAQFASPEILQRSLHNSGAEVVTVSLSRQMPEIGGGQDYWDIIKQSKCRVLPNTAGCYTVKDAV
LMAQMAREIFNTTWIKLEIIGDDYTLHPDVVALCEAAKELITQGFDVFPYCTDDLTVCRRLIDCGCRVIMPGISPIGSGL
GVLNMHGLRLLRNRYKDIVLIVDAGIGRPSDASVVMEQGIDGVLLNSAVARAIDPSKMASAFRYAIECGRLGYEAGIINK
RDLAVASTNLVDTPFWHRNKS

Sequences:

>Translated_261_residues
MWDVYGVQLRSRLLMGSAQFASPEILQRSLHNSGAEVVTVSLSRQMPEIGGGQDYWDIIKQSKCRVLPNTAGCYTVKDAV
LMAQMAREIFNTTWIKLEIIGDDYTLHPDVVALCEAAKELITQGFDVFPYCTDDLTVCRRLIDCGCRVIMPGISPIGSGL
GVLNMHGLRLLRNRYKDIVLIVDAGIGRPSDASVVMEQGIDGVLLNSAVARAIDPSKMASAFRYAIECGRLGYEAGIINK
RDLAVASTNLVDTPFWHRNKS
>Mature_261_residues
MWDVYGVQLRSRLLMGSAQFASPEILQRSLHNSGAEVVTVSLSRQMPEIGGGQDYWDIIKQSKCRVLPNTAGCYTVKDAV
LMAQMAREIFNTTWIKLEIIGDDYTLHPDVVALCEAAKELITQGFDVFPYCTDDLTVCRRLIDCGCRVIMPGISPIGSGL
GVLNMHGLRLLRNRYKDIVLIVDAGIGRPSDASVVMEQGIDGVLLNSAVARAIDPSKMASAFRYAIECGRLGYEAGIINK
RDLAVASTNLVDTPFWHRNKS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=241, Percent_Identity=37.344398340249, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28712; Mature: 28712

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDVYGVQLRSRLLMGSAQFASPEILQRSLHNSGAEVVTVSLSRQMPEIGGGQDYWDIIK
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHH
QSKCRVLPNTAGCYTVKDAVLMAQMAREIFNTTWIKLEIIGDDYTLHPDVVALCEAAKEL
CCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEECHHHHHHHHHHHHH
ITQGFDVFPYCTDDLTVCRRLIDCGCRVIMPGISPIGSGLGVLNMHGLRLLRNRYKDIVL
HHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
IVDAGIGRPSDASVVMEQGIDGVLLNSAVARAIDPSKMASAFRYAIECGRLGYEAGIINK
EEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
RDLAVASTNLVDTPFWHRNKS
CCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MWDVYGVQLRSRLLMGSAQFASPEILQRSLHNSGAEVVTVSLSRQMPEIGGGQDYWDIIK
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHH
QSKCRVLPNTAGCYTVKDAVLMAQMAREIFNTTWIKLEIIGDDYTLHPDVVALCEAAKEL
CCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEECHHHHHHHHHHHHH
ITQGFDVFPYCTDDLTVCRRLIDCGCRVIMPGISPIGSGLGVLNMHGLRLLRNRYKDIVL
HHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
IVDAGIGRPSDASVVMEQGIDGVLLNSAVARAIDPSKMASAFRYAIECGRLGYEAGIINK
EEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
RDLAVASTNLVDTPFWHRNKS
CCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA