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The map label for this gene is gltX

Identifier: 88607805

GI number: 88607805

Start: 79102

End: 80454

Strand: Direct

Name: gltX

Synonym: APH_0072

Alternate gene names: 88607805

Gene position: 79102-80454 (Clockwise)

Preceding gene: 88607265

Following gene: 88606731

Centisome position: 5.38

GC content: 41.76

Gene sequence:

>1353_bases
ATGATAACAAGATTTGCTCCAAGTCCCACTGGATACATGCATGTGGGAAATGCTAGAACGGCTTTGATATGTTGGCTGTA
TGCAAGAAGTAAATCGGGGAAGTTTCTGCTGCGCATTGACGATACGGATGCCAGTAGATCTGAGCATAAATACATTGAGG
GAATAAAGCAGGATCTGGATTGGCTTGCGCTGGATTGGGATTCTTGTTTTCAGCAGAGCACGAGGCTTGAACGATATCAA
GAAGTATTCGATTTGTTGCTAGATCAGGGTGTCATCTACCCCTGCTATGAAACTCAGGAAGAATTGGATATGAAGAGAAG
TATGATGTTGAAGATGGGATTACCTCCTATATACGACAGAAGTGCTCTCAAGATGACGGAGCAGGAAATGCAAGCTTGCA
GTGGCAGATTGCCGTACTTTAGGTTAAAGATTGATCAAAGCAGGGAAATCACTTGGGAAGATGAAGTGCGGGGTAGGGTA
TCATTTCAAGCGAAAAATATAAGTGATCCGATAATTAAGCGTACAGATGGTACATACACATACATGTTTCCGTCAGTGGT
AGATGATATAGATTTTGCTATCACACATATTATTCGGGGTGAAGACCACGTAAGCAATACGGCAACACAAATATGTATTT
TCGATATCTTAAAAGCAAAAGTTCCCGTATTTGTGCACTTACCTCTTGTCCATTTTCGTGATGCAAAAATTTCTAAGAGA
GTAGGTAGTGACGATATTGAAATACGGCACCTAAGGGATATTGGTATGGAACCTATGGCTATAAAAAGCTACTTAGCTAG
GATGGGGACATCATTACCAGTGGAACCACAGGAGAATCATGATGTTCTGGTAGAGTCGTTCGATATACGCACGTTCAACC
AAGCCCCAATTAAGTTTTCGCTTGATGATATAAGCAGATTGAACAGCAGAATAGTACAGTGTTTATCATTTGATAAAGTG
AAAGATAGGTTTGTGCAGCAAGGTTTGGAGTGCACAGAGGAGTTTTGGTACCTGATACGAGATAATGTTAACACAGTAGA
AGATGTGAGAGAGTGGATTAACATCTGTAATTCTCAAATCACAACTGCGATAGATGATAAAGATAGGACTTTTATTTCTG
AGGCCAAGGCACTTTTACCTGATACTGAGCTTGATGAAGAAGTTTGTAAGGCTTGGTTGCAGAGGATAAAGGAGACGTCT
AATCGCAGTACGCGGGATGTACTTTTACCACTGAGACTTGCCACCACTGGGGTTACTACTGGTCCGGGGTTAGCTCAGCT
GTTACCGTTTATTGGCAGGGCAGAGGTAGTAAGAAGGCTTGAATGTGCTAGTAAAGGGCATAATGCCAATTGA

Upstream 100 bases:

>100_bases
CGGGTGATATAAATGCCAGGGTTTTTCAGGTTGTTGTGATTACGGGATGAAATTCTGGTATTTACAGCCGGACGTTGCTG
TGTTGCTATAGAGGTTAGAT

Downstream 100 bases:

>100_bases
TATGGGGTAAGATCCTATAGCAATTTAAGAGAGTATAGTGGTGTGATATTGCAGAAGGGGAGCTTTGCAGGGGCTTTTGG
TTGTTTAGGTATGTTGATGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MITRFAPSPTGYMHVGNARTALICWLYARSKSGKFLLRIDDTDASRSEHKYIEGIKQDLDWLALDWDSCFQQSTRLERYQ
EVFDLLLDQGVIYPCYETQEELDMKRSMMLKMGLPPIYDRSALKMTEQEMQACSGRLPYFRLKIDQSREITWEDEVRGRV
SFQAKNISDPIIKRTDGTYTYMFPSVVDDIDFAITHIIRGEDHVSNTATQICIFDILKAKVPVFVHLPLVHFRDAKISKR
VGSDDIEIRHLRDIGMEPMAIKSYLARMGTSLPVEPQENHDVLVESFDIRTFNQAPIKFSLDDISRLNSRIVQCLSFDKV
KDRFVQQGLECTEEFWYLIRDNVNTVEDVREWINICNSQITTAIDDKDRTFISEAKALLPDTELDEEVCKAWLQRIKETS
NRSTRDVLLPLRLATTGVTTGPGLAQLLPFIGRAEVVRRLECASKGHNAN

Sequences:

>Translated_450_residues
MITRFAPSPTGYMHVGNARTALICWLYARSKSGKFLLRIDDTDASRSEHKYIEGIKQDLDWLALDWDSCFQQSTRLERYQ
EVFDLLLDQGVIYPCYETQEELDMKRSMMLKMGLPPIYDRSALKMTEQEMQACSGRLPYFRLKIDQSREITWEDEVRGRV
SFQAKNISDPIIKRTDGTYTYMFPSVVDDIDFAITHIIRGEDHVSNTATQICIFDILKAKVPVFVHLPLVHFRDAKISKR
VGSDDIEIRHLRDIGMEPMAIKSYLARMGTSLPVEPQENHDVLVESFDIRTFNQAPIKFSLDDISRLNSRIVQCLSFDKV
KDRFVQQGLECTEEFWYLIRDNVNTVEDVREWINICNSQITTAIDDKDRTFISEAKALLPDTELDEEVCKAWLQRIKETS
NRSTRDVLLPLRLATTGVTTGPGLAQLLPFIGRAEVVRRLECASKGHNAN
>Mature_450_residues
MITRFAPSPTGYMHVGNARTALICWLYARSKSGKFLLRIDDTDASRSEHKYIEGIKQDLDWLALDWDSCFQQSTRLERYQ
EVFDLLLDQGVIYPCYETQEELDMKRSMMLKMGLPPIYDRSALKMTEQEMQACSGRLPYFRLKIDQSREITWEDEVRGRV
SFQAKNISDPIIKRTDGTYTYMFPSVVDDIDFAITHIIRGEDHVSNTATQICIFDILKAKVPVFVHLPLVHFRDAKISKR
VGSDDIEIRHLRDIGMEPMAIKSYLARMGTSLPVEPQENHDVLVESFDIRTFNQAPIKFSLDDISRLNSRIVQCLSFDKV
KDRFVQQGLECTEEFWYLIRDNVNTVEDVREWINICNSQITTAIDDKDRTFISEAKALLPDTELDEEVCKAWLQRIKETS
NRSTRDVLLPLRLATTGVTTGPGLAQLLPFIGRAEVVRRLECASKGHNAN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=488, Percent_Identity=26.844262295082, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI1788743, Length=473, Percent_Identity=32.5581395348837, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI226510925, Length=243, Percent_Identity=33.7448559670782, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786895, Length=173, Percent_Identity=29.4797687861272, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=492, Percent_Identity=26.6260162601626, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324540, Length=320, Percent_Identity=31.5625, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321192, Length=309, Percent_Identity=23.6245954692557, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=31.5384615384615, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=485, Percent_Identity=30.9278350515464, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17864488, Length=103, Percent_Identity=32.0388349514563, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_ANAPZ (Q2GLP6)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504705.1
- ProteinModelPortal:   Q2GLP6
- SMR:   Q2GLP6
- STRING:   Q2GLP6
- GeneID:   3930848
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0072
- NMPDR:   fig|212042.5.peg.69
- TIGR:   APH_0072
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   RSKQEYI
- ProtClustDB:   PRK12558
- BioCyc:   APHA212042:APH_0072-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51851; Mature: 51851

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRFAPSPTGYMHVGNARTALICWLYARSKSGKFLLRIDDTDASRSEHKYIEGIKQDLD
CCCCCCCCCCCEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
WLALDWDSCFQQSTRLERYQEVFDLLLDQGVIYPCYETQEELDMKRSMMLKMGLPPIYDR
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCC
SALKMTEQEMQACSGRLPYFRLKIDQSREITWEDEVRGRVSFQAKNISDPIIKRTDGTYT
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCEEEEEECCCCCCHHCCCCCCEE
YMFPSVVDDIDFAITHIIRGEDHVSNTATQICIFDILKAKVPVFVHLPLVHFRDAKISKR
EECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCHHHHH
VGSDDIEIRHLRDIGMEPMAIKSYLARMGTSLPVEPQENHDVLVESFDIRTFNQAPIKFS
CCCCCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEECCCCCCEEE
LDDISRLNSRIVQCLSFDKVKDRFVQQGLECTEEFWYLIRDNVNTVEDVREWINICNSQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
TTAIDDKDRTFISEAKALLPDTELDEEVCKAWLQRIKETSNRSTRDVLLPLRLATTGVTT
EEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEEEHHHCCCCC
GPGLAQLLPFIGRAEVVRRLECASKGHNAN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITRFAPSPTGYMHVGNARTALICWLYARSKSGKFLLRIDDTDASRSEHKYIEGIKQDLD
CCCCCCCCCCCEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
WLALDWDSCFQQSTRLERYQEVFDLLLDQGVIYPCYETQEELDMKRSMMLKMGLPPIYDR
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCC
SALKMTEQEMQACSGRLPYFRLKIDQSREITWEDEVRGRVSFQAKNISDPIIKRTDGTYT
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCEEEEEECCCCCCHHCCCCCCEE
YMFPSVVDDIDFAITHIIRGEDHVSNTATQICIFDILKAKVPVFVHLPLVHFRDAKISKR
EECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCHHHHH
VGSDDIEIRHLRDIGMEPMAIKSYLARMGTSLPVEPQENHDVLVESFDIRTFNQAPIKFS
CCCCCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEECCCCCCEEE
LDDISRLNSRIVQCLSFDKVKDRFVQQGLECTEEFWYLIRDNVNTVEDVREWINICNSQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
TTAIDDKDRTFISEAKALLPDTELDEEVCKAWLQRIKETSNRSTRDVLLPLRLATTGVTT
EEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEEEHHHCCCCC
GPGLAQLLPFIGRAEVVRRLECASKGHNAN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA