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The map label for this gene is xth [H]

Identifier: 88607141

GI number: 88607141

Start: 531793

End: 532614

Strand: Reverse

Name: xth [H]

Synonym: APH_0505

Alternate gene names: 88607141

Gene position: 532614-531793 (Counterclockwise)

Preceding gene: 88606954

Following gene: 88606902

Centisome position: 36.2

GC content: 40.27

Gene sequence:

>822_bases
ATGATCAGAGTAATAACATGGAATGTCAATTCGATAAGAAAAAGAATAGAGCACCTGTGTAGTGTGCTATCAGAGCACAG
TATAGACGTTGCTATGCTACAAGAAATCAAGTGCACAAATGAGCAATTCCCTTTCGTTGAGCTAGAAGCACTAGGTTATA
AATGCTACGTGCATGGTCAGAAATCTAGAAATGGAGTTGCGATAATAAGTAAGTTGCCCGTAGTAGAAGTAGTTTCCTAC
AGCGTTTTAGACGAAGGAAAGGAGTTGGAAAGCGCAGGAAGTTATTCATCTGAGGAATCAAGATATATAGAGTGTACACT
GGAGTGTACTGGGAATAAGAAAATAAGAGTAGTGAGCGTCTACGTTCCCAACGGGCAGGAGGTAGAATCTGAGACGTTTT
TCTATAAATTGAAATTCCTGGAACATCTGAAGGACAGATTGTTAAACATCATGAAAACAGAGGACTTCCTCATTGCTGGG
GGTGACTTCAATGTTGCACCTGAAGAGATAGACGTGCACGATCCTAAAGCTCTTGATGGCAGGCTATGCTTTCATATTTT
AGAGCGTGCTAAATTCAGAGAAATTCTAAATAACGGTATAGTGGATATTTTTCGCACTTTTGTGGGTATTGATAGAAAAG
AGTTTAGTTGGTGGAATTACAGAGAAGGCGGATGGCAGAATAATAGAGGTCTTAGAATAGACGCTCTGCTATCTTCTCCT
CAAATCGCTGATAAAGTTCTAGATTGTTCTATACTATCGAAGGTTAGAGGTTGGGATACTCCTTCGGATCATGCTCCGGT
TATGGGCGACATAGATGTCTAA

Upstream 100 bases:

>100_bases
TACGCTTAGGAATCGCATCCTTGGTAAAAGATCGACTTGACATGCTCAGTAGCTTACTATCTAACATCCTAATACATAAT
TAAAACGTTTAGTGTGCGGT

Downstream 100 bases:

>100_bases
TGTGGGTTCATGTGCTTAATTCTTGGTGCTATTTGGGGTTTTGATACGTATAAGTTGTGGGGTGTCTTGACTCTCTTATG
AGGTGTTTTTAAGGTATTGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIRVITWNVNSIRKRIEHLCSVLSEHSIDVAMLQEIKCTNEQFPFVELEALGYKCYVHGQKSRNGVAIISKLPVVEVVSY
SVLDEGKELESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAG
GDFNVAPEEIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSP
QIADKVLDCSILSKVRGWDTPSDHAPVMGDIDV

Sequences:

>Translated_273_residues
MIRVITWNVNSIRKRIEHLCSVLSEHSIDVAMLQEIKCTNEQFPFVELEALGYKCYVHGQKSRNGVAIISKLPVVEVVSY
SVLDEGKELESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAG
GDFNVAPEEIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSP
QIADKVLDCSILSKVRGWDTPSDHAPVMGDIDV
>Mature_273_residues
MIRVITWNVNSIRKRIEHLCSVLSEHSIDVAMLQEIKCTNEQFPFVELEALGYKCYVHGQKSRNGVAIISKLPVVEVVSY
SVLDEGKELESAGSYSSEESRYIECTLECTGNKKIRVVSVYVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAG
GDFNVAPEEIDVHDPKALDGRLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSP
QIADKVLDCSILSKVRGWDTPSDHAPVMGDIDV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=274, Percent_Identity=27.3722627737226, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI18375503, Length=274, Percent_Identity=27.3722627737226, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI18375501, Length=274, Percent_Identity=27.3722627737226, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788046, Length=275, Percent_Identity=30.5454545454545, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71989536, Length=281, Percent_Identity=30.2491103202847, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=29.0909090909091, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=29.4545454545455, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31192; Mature: 31192

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVITWNVNSIRKRIEHLCSVLSEHSIDVAMLQEIKCTNEQFPFVELEALGYKCYVHGQ
CEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEECC
KSRNGVAIISKLPVVEVVSYSVLDEGKELESAGSYSSEESRYIECTLECTGNKKIRVVSV
CCCCCEEEEECCCHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEEEE
YVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAGGDFNVAPEEIDVHDPKALDG
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCH
RLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSP
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCC
QIADKVLDCSILSKVRGWDTPSDHAPVMGDIDV
HHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MIRVITWNVNSIRKRIEHLCSVLSEHSIDVAMLQEIKCTNEQFPFVELEALGYKCYVHGQ
CEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEECC
KSRNGVAIISKLPVVEVVSYSVLDEGKELESAGSYSSEESRYIECTLECTGNKKIRVVSV
CCCCCEEEEECCCHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEEEE
YVPNGQEVESETFFYKLKFLEHLKDRLLNIMKTEDFLIAGGDFNVAPEEIDVHDPKALDG
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCH
RLCFHILERAKFREILNNGIVDIFRTFVGIDRKEFSWWNYREGGWQNNRGLRIDALLSSP
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCC
QIADKVLDCSILSKVRGWDTPSDHAPVMGDIDV
HHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]