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The map label for this gene is pchF [H]

Identifier: 87200974

GI number: 87200974

Start: 3178547

End: 3180175

Strand: Direct

Name: pchF [H]

Synonym: Saro_2962

Alternate gene names: 87200974

Gene position: 3178547-3180175 (Clockwise)

Preceding gene: 87200973

Following gene: 87200975

Centisome position: 89.25

GC content: 65.56

Gene sequence:

>1629_bases
ATGAGCGAATTGAACCTCGACCGTCGCGGCCTTCTTGGTGCGGGCCTTGTCGGCGCGGCCAGCCTCGGCCTCGGCACGGG
CGCCAGCGCGAAGAACCCCGCCCCGCTCGCCCCGCACATGACCAAGGCCGATTTCGCGGGCGCGATGAAGGCATTTCGCG
GCGTCGTCGGCGCCGAATGGGTGTTCGGCGACGAGGAGGCCGTTGCGCCCTACACCAAGGTCTACGTGCCCGATCCCGCC
AACCGCCATGTGCCGATCGGCGCCGTCTGCCCGGAATCGGTGGAGCAGGTGCAGGAAATCGTCCGCATCGCCAACAAGTA
CCGCCAGCCGCTGTGGCCAGTCTCCACTGGCAAGAACATGGGCTATGGCATGACCGCGCCGGCAACGCCGGGCCAGGTCG
TGCTCGACCTCAAGCGGATGAACCGCATTCTCGAGGTCGACGCGGACCTCGGCACTTGCCTGCTGGAGCCGGGCGTCACC
TACCAGCAGCTCAAGGACTACCTTGTAGAGAACAACATCCCGCTGTGGATCGACGTGCCGACAGTGGGCCCGGTGGCCTC
GCCGGTGGGCAACACGCTCGACCGCGGGGTGGGCTACACGCCTTATGGCGAACACTTCATGTTCCAGTGCGGCATGGAAG
TCGTACTCGCCGACGGTCAGGTCATGCGCACCGGCATGGGCTCGATCAAGGGCAGCACCGCGTGGCAGGCGTTCAAGTGG
GGCTACGGCCCTTATCTCGACGGCCTTTTCACCCAGTCGAACTTCGGCGTGGTCACCAAGATGGGCTTGTGGCTGATGCC
CAGGCCCCCGGTCTACAAGCCTTTCATGGTTCGCCATGGCGAGATGGCCGACGTCCCGCGCATCATCGAGGCGATGCGCC
CGCTTCGTGTCTCGAACCTCGTCGCCAATTGCAACCTGATGATGAGCGCGTCCTACCAGCTTGCCATGTTCAAGCGCCGC
AACGAGATCGTCGCTGACGGCGTGCCGCTCGATGATGCCTCGCTCAAGAAGGTGGCCAAGGCCAACGGCCTGGGCATGTG
GAACACCTACTTCGCGCTCTACGGCACCGAACAGACCGTCGCGGCGATCGAGCCGATCATCCGCGCGAGCCTTGTGGCAA
GCGGCGGCGAAGTGCTGACCGCCGCCGAGATGGGCGACAACCCCTGGTTCCACCACCACGCCACGCTGATGGAAGGCGGG
CTCAATCTCGACGAGGTCGGCCTGCTGCGCTGGCGCGGTGCGGGCGGTGGCCTCGCCTGGTTCGCCCCCGTCGCCGCCGC
GCGAGGGATCGAGGCCGAGCGACAGACCGCGCTCGCCAGGGAAATCCTCGAGAAGCACGGCTTCGACTATACCGCCGCCT
ACGCCATCGGCTGGCGCGACCTGCATCACATCATCGCCCTGCTGTTCGACAAATCCGATGCCGATCAGGAACGCAAGGCT
GACGCCTGCTACCGCGAACTGGTCACCCGCTTCGGCGCGCAAGGCTGGGCGAGCTACCGCACCGGGGTCAATTCGATGGA
CCTCGTCGCGCAGCAGTACGGGCAGGTGAACCGCGAGTTCAACGCGAAGATCAAGCATGCCGTCGATCCAAACGGCATCC
TTGCTCCCGGCAAATCGGGGATTGTGTGA

Upstream 100 bases:

>100_bases
GGATGCCCGCATTCCGCCCGACCGAGGTGAAGCCGGAAGAGCTGGATGCGCTCGCGGCGTGGATCGAGAAGAGCGAAGCG
CGCAAGGGGGAGCACGGGCA

Downstream 100 bases:

>100_bases
TGATGCGCTTCGATGGAAAGGCAGTCCTCGTGACGGGGGCGGCGACCGGCATCGGCAGGGCCACCGCCCTCGCCTTCGCT
CGCGAAGGGGCCTCGGTCAT

Product: twin-arginine translocation pathway signal protein

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSELNLDRRGLLGAGLVGAASLGLGTGASAKNPAPLAPHMTKADFAGAMKAFRGVVGAEWVFGDEEAVAPYTKVYVPDPA
NRHVPIGAVCPESVEQVQEIVRIANKYRQPLWPVSTGKNMGYGMTAPATPGQVVLDLKRMNRILEVDADLGTCLLEPGVT
YQQLKDYLVENNIPLWIDVPTVGPVASPVGNTLDRGVGYTPYGEHFMFQCGMEVVLADGQVMRTGMGSIKGSTAWQAFKW
GYGPYLDGLFTQSNFGVVTKMGLWLMPRPPVYKPFMVRHGEMADVPRIIEAMRPLRVSNLVANCNLMMSASYQLAMFKRR
NEIVADGVPLDDASLKKVAKANGLGMWNTYFALYGTEQTVAAIEPIIRASLVASGGEVLTAAEMGDNPWFHHHATLMEGG
LNLDEVGLLRWRGAGGGLAWFAPVAAARGIEAERQTALAREILEKHGFDYTAAYAIGWRDLHHIIALLFDKSDADQERKA
DACYRELVTRFGAQGWASYRTGVNSMDLVAQQYGQVNREFNAKIKHAVDPNGILAPGKSGIV

Sequences:

>Translated_542_residues
MSELNLDRRGLLGAGLVGAASLGLGTGASAKNPAPLAPHMTKADFAGAMKAFRGVVGAEWVFGDEEAVAPYTKVYVPDPA
NRHVPIGAVCPESVEQVQEIVRIANKYRQPLWPVSTGKNMGYGMTAPATPGQVVLDLKRMNRILEVDADLGTCLLEPGVT
YQQLKDYLVENNIPLWIDVPTVGPVASPVGNTLDRGVGYTPYGEHFMFQCGMEVVLADGQVMRTGMGSIKGSTAWQAFKW
GYGPYLDGLFTQSNFGVVTKMGLWLMPRPPVYKPFMVRHGEMADVPRIIEAMRPLRVSNLVANCNLMMSASYQLAMFKRR
NEIVADGVPLDDASLKKVAKANGLGMWNTYFALYGTEQTVAAIEPIIRASLVASGGEVLTAAEMGDNPWFHHHATLMEGG
LNLDEVGLLRWRGAGGGLAWFAPVAAARGIEAERQTALAREILEKHGFDYTAAYAIGWRDLHHIIALLFDKSDADQERKA
DACYRELVTRFGAQGWASYRTGVNSMDLVAQQYGQVNREFNAKIKHAVDPNGILAPGKSGIV
>Mature_541_residues
SELNLDRRGLLGAGLVGAASLGLGTGASAKNPAPLAPHMTKADFAGAMKAFRGVVGAEWVFGDEEAVAPYTKVYVPDPAN
RHVPIGAVCPESVEQVQEIVRIANKYRQPLWPVSTGKNMGYGMTAPATPGQVVLDLKRMNRILEVDADLGTCLLEPGVTY
QQLKDYLVENNIPLWIDVPTVGPVASPVGNTLDRGVGYTPYGEHFMFQCGMEVVLADGQVMRTGMGSIKGSTAWQAFKWG
YGPYLDGLFTQSNFGVVTKMGLWLMPRPPVYKPFMVRHGEMADVPRIIEAMRPLRVSNLVANCNLMMSASYQLAMFKRRN
EIVADGVPLDDASLKKVAKANGLGMWNTYFALYGTEQTVAAIEPIIRASLVASGGEVLTAAEMGDNPWFHHHATLMEGGL
NLDEVGLLRWRGAGGGLAWFAPVAAARGIEAERQTALAREILEKHGFDYTAAYAIGWRDLHHIIALLFDKSDADQERKAD
ACYRELVTRFGAQGWASYRTGVNSMDLVAQQYGQVNREFNAKIKHAVDPNGILAPGKSGIV

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 58885; Mature: 58754

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNLDRRGLLGAGLVGAASLGLGTGASAKNPAPLAPHMTKADFAGAMKAFRGVVGAEW
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
VFGDEEAVAPYTKVYVPDPANRHVPIGAVCPESVEQVQEIVRIANKYRQPLWPVSTGKNM
EECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GYGMTAPATPGQVVLDLKRMNRILEVDADLGTCLLEPGVTYQQLKDYLVENNIPLWIDVP
CCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEEECCCCCHHHHHHHHHHCCCCEEEECC
TVGPVASPVGNTLDRGVGYTPYGEHFMFQCGMEVVLADGQVMRTGMGSIKGSTAWQAFKW
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCEEECCCCCCCCCCHHHHHHC
GYGPYLDGLFTQSNFGVVTKMGLWLMPRPPVYKPFMVRHGEMADVPRIIEAMRPLRVSNL
CCCCHHCCCEECCCCCEEEECCEEECCCCCCCCCHHHCCCCCCCHHHHHHHHCCHHHHHH
VANCNLMMSASYQLAMFKRRNEIVADGVPLDDASLKKVAKANGLGMWNTYFALYGTEQTV
HHCCEEEEECCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHH
AAIEPIIRASLVASGGEVLTAAEMGDNPWFHHHATLMEGGLNLDEVGLLRWRGAGGGLAW
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHH
FAPVAAARGIEAERQTALAREILEKHGFDYTAAYAIGWRDLHHIIALLFDKSDADQERKA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHH
DACYRELVTRFGAQGWASYRTGVNSMDLVAQQYGQVNREFNAKIKHAVDPNGILAPGKSG
HHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCHHCCEEEECCCCCCEECCCCCC
IV
CC
>Mature Secondary Structure 
SELNLDRRGLLGAGLVGAASLGLGTGASAKNPAPLAPHMTKADFAGAMKAFRGVVGAEW
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
VFGDEEAVAPYTKVYVPDPANRHVPIGAVCPESVEQVQEIVRIANKYRQPLWPVSTGKNM
EECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GYGMTAPATPGQVVLDLKRMNRILEVDADLGTCLLEPGVTYQQLKDYLVENNIPLWIDVP
CCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEEECCCCCHHHHHHHHHHCCCCEEEECC
TVGPVASPVGNTLDRGVGYTPYGEHFMFQCGMEVVLADGQVMRTGMGSIKGSTAWQAFKW
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCEEECCCCCCCCCCHHHHHHC
GYGPYLDGLFTQSNFGVVTKMGLWLMPRPPVYKPFMVRHGEMADVPRIIEAMRPLRVSNL
CCCCHHCCCEECCCCCEEEECCEEECCCCCCCCCHHHCCCCCCCHHHHHHHHCCHHHHHH
VANCNLMMSASYQLAMFKRRNEIVADGVPLDDASLKKVAKANGLGMWNTYFALYGTEQTV
HHCCEEEEECCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHH
AAIEPIIRASLVASGGEVLTAAEMGDNPWFHHHATLMEGGLNLDEVGLLRWRGAGGGLAW
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHH
FAPVAAARGIEAERQTALAREILEKHGFDYTAAYAIGWRDLHHIIALLFDKSDADQERKA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHH
DACYRELVTRFGAQGWASYRTGVNSMDLVAQQYGQVNREFNAKIKHAVDPNGILAPGKSG
HHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCHHCCEEEECCCCCCEECCCCCC
IV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]