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The map label for this gene is ada [H]

Identifier: 87200409

GI number: 87200409

Start: 2563138

End: 2564169

Strand: Reverse

Name: ada [H]

Synonym: Saro_2396

Alternate gene names: 87200409

Gene position: 2564169-2563138 (Counterclockwise)

Preceding gene: 87200410

Following gene: 87200408

Centisome position: 72.0

GC content: 69.86

Gene sequence:

>1032_bases
GTGATTGCCGAGGACGAGGCATGGACTGCCGTTCTGGCACGCGACCGCGCCTTCGACGGGCGCTTCGTCACCGGCGTGCT
GAGCACGGGAATCTACTGCCGGCCTTCGTGTTCCGCCCGCCATCCGCGTCGCGAGAACGTGCGGTTCTTTGCCGACGGAG
AGGCCGCGCGCGCGGCGGGGCTGCGTCCGTGCCTGCGGTGCTCGCCCGACGAGGTATCGCGCGACGAACGCGCCGTGCTC
ATGGCGATCGAGGCGATCAAGGCGGCGGGGCAGACGCTGGCGCTTGCCGACCTTGCCGAGCAGTGCGGTTATTCACCGAC
GCATTTCCAGCGGGTCTTCGCCCGCGCCACCGGGCTTTCGCCCGCCGCCTACGCCCGGGCCTTGCAGAAGGAACGCATGG
CCGAGGTGCTGACAGGAGCGGAGCGTGTGACCGACGCCGTATATGACGCGGGCTTTTCCGGCCCGTCGCGGTTCTATTCG
GCGGCCGGGGACAGGCTGGGCATGAGCCCTTCGGCCTGGCGCGACGGTGGGCGAGGGGTGACGATCCGCTGGGCCGTGGT
GGCGACTTCGCTGGGGCCGATGCTGGTGGCGGCGACCGACAAGGGCGTTTGCCGGCTGTCCTTCAACGAGGACAGCGAGG
CTTTGCGCCGCCGGTTCCCGAATGCCGACCTGGCCGAGGGCGGGGAGGCATTCGAGCGGCTTGTCGGCGATGTCGTTGCC
GCCGTCGAGGCGCCGGGCGACCATTCGCACATCCCCCTGGACGTGAAGGGGACGGCCTTTCAGGAAGCGGTGTGGCAGGC
CCTGCGGCGCATCCCTCCCGGCGAGACGCGGTCCTATGCCGAGATCGCGGCGGAAGTGGGCAAGCCGGGCGCGGTGCGCG
CGGCGGGATCGGCCAATGGCGCGAACAACGTGGCGGTGCTGATCCCGTGCCATCGCGTGATCCGTTCCGACGGGTCGCTG
GGCGGCTATGCCTATGGGCTTGAGATCAAGCGGCGGTTGCTGGACAAGGAACGCGACGCGAAGGGGGAATGA

Upstream 100 bases:

>100_bases
ATCTGCTTGCCGATCCGCGCATTGCCACTATCCATGCTCACAACGCCGCGCATGGCTGCTTCGTCGCGCGGATCGATCGA
GACGACACGGGAGACGCAGC

Downstream 100 bases:

>100_bases
CGATGGACGAGGCGCAGACCAGCAAGCCCGGAACGCTGCGGCGGATCTTCGACTTTCCCGCAACCCCGATGGTGATCGAG
TTCCTGGCGGTGGGGCTCTT

Product: transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MIAEDEAWTAVLARDRAFDGRFVTGVLSTGIYCRPSCSARHPRRENVRFFADGEAARAAGLRPCLRCSPDEVSRDERAVL
MAIEAIKAAGQTLALADLAEQCGYSPTHFQRVFARATGLSPAAYARALQKERMAEVLTGAERVTDAVYDAGFSGPSRFYS
AAGDRLGMSPSAWRDGGRGVTIRWAVVATSLGPMLVAATDKGVCRLSFNEDSEALRRRFPNADLAEGGEAFERLVGDVVA
AVEAPGDHSHIPLDVKGTAFQEAVWQALRRIPPGETRSYAEIAAEVGKPGAVRAAGSANGANNVAVLIPCHRVIRSDGSL
GGYAYGLEIKRRLLDKERDAKGE

Sequences:

>Translated_343_residues
MIAEDEAWTAVLARDRAFDGRFVTGVLSTGIYCRPSCSARHPRRENVRFFADGEAARAAGLRPCLRCSPDEVSRDERAVL
MAIEAIKAAGQTLALADLAEQCGYSPTHFQRVFARATGLSPAAYARALQKERMAEVLTGAERVTDAVYDAGFSGPSRFYS
AAGDRLGMSPSAWRDGGRGVTIRWAVVATSLGPMLVAATDKGVCRLSFNEDSEALRRRFPNADLAEGGEAFERLVGDVVA
AVEAPGDHSHIPLDVKGTAFQEAVWQALRRIPPGETRSYAEIAAEVGKPGAVRAAGSANGANNVAVLIPCHRVIRSDGSL
GGYAYGLEIKRRLLDKERDAKGE
>Mature_343_residues
MIAEDEAWTAVLARDRAFDGRFVTGVLSTGIYCRPSCSARHPRRENVRFFADGEAARAAGLRPCLRCSPDEVSRDERAVL
MAIEAIKAAGQTLALADLAEQCGYSPTHFQRVFARATGLSPAAYARALQKERMAEVLTGAERVTDAVYDAGFSGPSRFYS
AAGDRLGMSPSAWRDGGRGVTIRWAVVATSLGPMLVAATDKGVCRLSFNEDSEALRRRFPNADLAEGGEAFERLVGDVVA
AVEAPGDHSHIPLDVKGTAFQEAVWQALRRIPPGETRSYAEIAAEVGKPGAVRAAGSANGANNVAVLIPCHRVIRSDGSL
GGYAYGLEIKRRLLDKERDAKGE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=95, Percent_Identity=40, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788542, Length=342, Percent_Identity=42.1052631578947, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1787596, Length=161, Percent_Identity=37.2670807453416, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=56.25, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=56.25, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=122, Percent_Identity=36.0655737704918, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137554, Length=173, Percent_Identity=32.9479768786127, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 36752; Mature: 36752

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEDEAWTAVLARDRAFDGRFVTGVLSTGIYCRPSCSARHPRRENVRFFADGEAARAAG
CCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCCCEEEECCCHHHHHC
LRPCLRCSPDEVSRDERAVLMAIEAIKAAGQTLALADLAEQCGYSPTHFQRVFARATGLS
CCHHHCCCCHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
PAAYARALQKERMAEVLTGAERVTDAVYDAGFSGPSRFYSAAGDRLGMSPSAWRDGGRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCE
TIRWAVVATSLGPMLVAATDKGVCRLSFNEDSEALRRRFPNADLAEGGEAFERLVGDVVA
EEEEEEEHHCCCCEEEEECCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AVEAPGDHSHIPLDVKGTAFQEAVWQALRRIPPGETRSYAEIAAEVGKPGAVRAAGSANG
HHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCCCCC
ANNVAVLIPCHRVIRSDGSLGGYAYGLEIKRRLLDKERDAKGE
CCCEEEEEECHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAEDEAWTAVLARDRAFDGRFVTGVLSTGIYCRPSCSARHPRRENVRFFADGEAARAAG
CCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCCCEEEECCCHHHHHC
LRPCLRCSPDEVSRDERAVLMAIEAIKAAGQTLALADLAEQCGYSPTHFQRVFARATGLS
CCHHHCCCCHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
PAAYARALQKERMAEVLTGAERVTDAVYDAGFSGPSRFYSAAGDRLGMSPSAWRDGGRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCE
TIRWAVVATSLGPMLVAATDKGVCRLSFNEDSEALRRRFPNADLAEGGEAFERLVGDVVA
EEEEEEEHHCCCCEEEEECCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AVEAPGDHSHIPLDVKGTAFQEAVWQALRRIPPGETRSYAEIAAEVGKPGAVRAAGSANG
HHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCCCCC
ANNVAVLIPCHRVIRSDGSLGGYAYGLEIKRRLLDKERDAKGE
CCCEEEEEECHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]