Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 87199973

GI number: 87199973

Start: 2074050

End: 2075417

Strand: Reverse

Name: radA [H]

Synonym: Saro_1956

Alternate gene names: 87199973

Gene position: 2075417-2074050 (Counterclockwise)

Preceding gene: 87199974

Following gene: 87199972

Centisome position: 58.27

GC content: 65.06

Gene sequence:

>1368_bases
ATGGCCAAACCCAAACGCCGATATGTGTGCCAGGCTTGTGGCAGCGTCACCCACCGCTGGCAGGGTCAGTGCGTCGATTG
CAGCGAGTGGAACACGCTGGCCGAAGAAGCACCCGAGACAATCTTCTCCGCCAAGCACGACCTGTCGAGCGGCGGCCGAC
GCGTCGCCTTCGTATCGCTCGACCAGCCGGTCGCGTTGCCGCAGCGCCGCAAGACGGGCATTGCCGAATTCGACCGTGCG
CTTGGGGGCGGCCTTGTCCCCGGATGCGCCATCCTGATGGGCGGCGACCCGGGGATCGGCAAGTCCACGCTCCTCCTCCA
GGCATCGGCGAAGGTCGCTCTATCGGGCGGAAGCGCGGTCTACATCAGCGGCGAGGAGGCATCGGATCAGGTGCGCCTGC
GTGCGGATCGCCTGGGCCTTGGCAAGGCACCGATCCTGCTCGCCGCAGCCACATCGGTTCGCGACATCCTCACGACGCTT
GGCAACATGGAGCCGCCATCGCTGGTGGTCATCGATTCCATCCAGACCATGCACTCCGACCAGATCGAGGGAGCGCCGGG
CACGGTCAGCCAGGTTCGCGGCTGCGCATTCGAACTGATCCGTTATGCCAAGGAGAACGGCGTCGCGCTTGTTCTCGTCG
GCCACGTCACCAAGGATGGTTCGATCGCCGGGCCCCGCGTGCTCGAACACATGGTCGATGTGGTGATGAGCTTCGAAGGG
GAACGCAGCCATCAGTACCGCATCCTCCGCGCACTGAAGAACCGCTTCGGCGCGGTGGACGAGATCGGCGTTTTCGCCAT
GGCAGGGCAGGGGCTTGAGGAAGTCGGCAATCCGTCGATGCTGTTCCTGTCGGGGCGTGAGCAGGAAGTGCCCGGTAGCG
CGGTCTTTCCCGCGCTCGAAGGGACGCGGCCAGTTCTGGTGGAAATCCAGGCTCTTACCGTCCGCCTTGCCAGTGGTGCC
ACCCCACGCCGCGCGGTCGTGGGCTGGGATAGCGGCCGCCTCGCCATGCTTCTTGCCGTGCTCGAGGCGCGCTGTGGTCT
CAATTTCTCTTCGGCTGAGGTCTATCTCAACGTCGCTGGAGGCTACCGGTTGACCGATCCTGCGGCAGACCTGGCGGTCG
CCGCAGCGCTTGTGTCGGCCCTGTCGGATCGCCCATTGCCGAGCGGATCGATCTGGTTCGGCGAGATTTCGCTTGCCGGC
GAGGTCCGCCCCGTGGCCCACACCCCGGTTCGTCTAAAGGAGGCGGCGAAGCTCGGTTTTTCAAGGGGCTGTGGTCCCGA
AGCCGGTGCGGAAAAGGTCGATGGACTGCGCTATTCGCCGCTCAGGCTGCTGCCAAATCTGATTGACCGGGTCATGGGCG
GCTCATAG

Upstream 100 bases:

>100_bases
CGAACCCGCTGACGTCGCGATGTGCGGAAGCGGGTACATATCGCTCTCGAGCGGTCGGGCGCAGCTTGTGTTCCAGATTT
GTTCCGGATAAGCACTGGGC

Downstream 100 bases:

>100_bases
GGTCCAGAGCCAATGACCGGGTTCGATTATGTGGTCCTTCTCCTCGTCGGCCTGGGTGCCGTCGGCGGCTTCTTTCGCGG
CTTCGTGGAGGAAGTGCTCT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAKPKRRYVCQACGSVTHRWQGQCVDCSEWNTLAEEAPETIFSAKHDLSSGGRRVAFVSLDQPVALPQRRKTGIAEFDRA
LGGGLVPGCAILMGGDPGIGKSTLLLQASAKVALSGGSAVYISGEEASDQVRLRADRLGLGKAPILLAAATSVRDILTTL
GNMEPPSLVVIDSIQTMHSDQIEGAPGTVSQVRGCAFELIRYAKENGVALVLVGHVTKDGSIAGPRVLEHMVDVVMSFEG
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVGNPSMLFLSGREQEVPGSAVFPALEGTRPVLVEIQALTVRLASGA
TPRRAVVGWDSGRLAMLLAVLEARCGLNFSSAEVYLNVAGGYRLTDPAADLAVAAALVSALSDRPLPSGSIWFGEISLAG
EVRPVAHTPVRLKEAAKLGFSRGCGPEAGAEKVDGLRYSPLRLLPNLIDRVMGGS

Sequences:

>Translated_455_residues
MAKPKRRYVCQACGSVTHRWQGQCVDCSEWNTLAEEAPETIFSAKHDLSSGGRRVAFVSLDQPVALPQRRKTGIAEFDRA
LGGGLVPGCAILMGGDPGIGKSTLLLQASAKVALSGGSAVYISGEEASDQVRLRADRLGLGKAPILLAAATSVRDILTTL
GNMEPPSLVVIDSIQTMHSDQIEGAPGTVSQVRGCAFELIRYAKENGVALVLVGHVTKDGSIAGPRVLEHMVDVVMSFEG
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVGNPSMLFLSGREQEVPGSAVFPALEGTRPVLVEIQALTVRLASGA
TPRRAVVGWDSGRLAMLLAVLEARCGLNFSSAEVYLNVAGGYRLTDPAADLAVAAALVSALSDRPLPSGSIWFGEISLAG
EVRPVAHTPVRLKEAAKLGFSRGCGPEAGAEKVDGLRYSPLRLLPNLIDRVMGGS
>Mature_454_residues
AKPKRRYVCQACGSVTHRWQGQCVDCSEWNTLAEEAPETIFSAKHDLSSGGRRVAFVSLDQPVALPQRRKTGIAEFDRAL
GGGLVPGCAILMGGDPGIGKSTLLLQASAKVALSGGSAVYISGEEASDQVRLRADRLGLGKAPILLAAATSVRDILTTLG
NMEPPSLVVIDSIQTMHSDQIEGAPGTVSQVRGCAFELIRYAKENGVALVLVGHVTKDGSIAGPRVLEHMVDVVMSFEGE
RSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVGNPSMLFLSGREQEVPGSAVFPALEGTRPVLVEIQALTVRLASGAT
PRRAVVGWDSGRLAMLLAVLEARCGLNFSSAEVYLNVAGGYRLTDPAADLAVAAALVSALSDRPLPSGSIWFGEISLAGE
VRPVAHTPVRLKEAAKLGFSRGCGPEAGAEKVDGLRYSPLRLLPNLIDRVMGGS

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=439, Percent_Identity=49.2027334851936, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 47998; Mature: 47867

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPKRRYVCQACGSVTHRWQGQCVDCSEWNTLAEEAPETIFSAKHDLSSGGRRVAFVSL
CCCCHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DQPVALPQRRKTGIAEFDRALGGGLVPGCAILMGGDPGIGKSTLLLQASAKVALSGGSAV
CCCCCCCCHHHCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCEEEECCCCEE
YISGEEASDQVRLRADRLGLGKAPILLAAATSVRDILTTLGNMEPPSLVVIDSIQTMHSD
EEECCCCCCCEEEEHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEECHHHHCCC
QIEGAPGTVSQVRGCAFELIRYAKENGVALVLVGHVTKDGSIAGPRVLEHMVDVVMSFEG
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVGNPSMLFLSGREQEVPGSAVFPALE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCCCCCEECCCCC
GTRPVLVEIQALTVRLASGATPRRAVVGWDSGRLAMLLAVLEARCGLNFSSAEVYLNVAG
CCCCEEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
GYRLTDPAADLAVAAALVSALSDRPLPSGSIWFGEISLAGEVRPVAHTPVRLKEAAKLGF
CEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCC
SRGCGPEAGAEKVDGLRYSPLRLLPNLIDRVMGGS
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKPKRRYVCQACGSVTHRWQGQCVDCSEWNTLAEEAPETIFSAKHDLSSGGRRVAFVSL
CCCHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DQPVALPQRRKTGIAEFDRALGGGLVPGCAILMGGDPGIGKSTLLLQASAKVALSGGSAV
CCCCCCCCHHHCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCEEEECCCCEE
YISGEEASDQVRLRADRLGLGKAPILLAAATSVRDILTTLGNMEPPSLVVIDSIQTMHSD
EEECCCCCCCEEEEHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEECHHHHCCC
QIEGAPGTVSQVRGCAFELIRYAKENGVALVLVGHVTKDGSIAGPRVLEHMVDVVMSFEG
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ERSHQYRILRALKNRFGAVDEIGVFAMAGQGLEEVGNPSMLFLSGREQEVPGSAVFPALE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCCCCCEECCCCC
GTRPVLVEIQALTVRLASGATPRRAVVGWDSGRLAMLLAVLEARCGLNFSSAEVYLNVAG
CCCCEEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
GYRLTDPAADLAVAAALVSALSDRPLPSGSIWFGEISLAGEVRPVAHTPVRLKEAAKLGF
CEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCC
SRGCGPEAGAEKVDGLRYSPLRLLPNLIDRVMGGS
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA