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The map label for this gene is proP [H]

Identifier: 87199970

GI number: 87199970

Start: 2071344

End: 2072978

Strand: Reverse

Name: proP [H]

Synonym: Saro_1953

Alternate gene names: 87199970

Gene position: 2072978-2071344 (Counterclockwise)

Preceding gene: 87199971

Following gene: 87199969

Centisome position: 58.2

GC content: 62.63

Gene sequence:

>1635_bases
ATGTGCGTGAATGCATCCGTAACGAAGAACGACGCCGCGAAGCCGAGCGCGTCGGATATCAGGCTCGTGATCGCCGCCAG
TTCGGCGGGTACCGTGTTCGAATGGTACGATTTCTTCATCTACGGCACGCTGGCATCAATCATCGGCAGGACGTTCTTTC
CCTCGGACAACGCAACGCTCCAGGTGCTGCTGGTGTGGGCGGGATTTGCCGTCGGCTTCGGCTTCCGTCCGCTCGGCGCA
GTCTTGTTCGGCTATCTCGGCGACAAGCTCGGCCGCAAGTATACCTTTCTCGTCACGGTCACGCTCATGGGTGTCGCCAC
GGCGGGCGTCGGCCTGATCCCCTCTGCCGCGACCATCGGCCTTGCCGCACCGGCCATCGTCATCCTGCTGCGCGTGCTGC
AAGGGCTGGCACTGGGTGGGGAGTACGGCGGCGCTGCGATCTATGTTGCAGAGCATGCACCGGGCGGCCGTCGCGGCTAT
TACACCAGCTACATCCAGGCCAGCGTCGTGGGCGGCTTCGTGCTGAGTCTGATCGTAGTCCTGTCCAGCAAGGCGCTGAT
GAGCGATGCCGTGTGGAACGACTGGGGTTGGCGCGTGCCGTTCCTGGTCAGCCTCGCGCTTCTCGCGATTTCGTTGTGGA
TGCGCATGAAGCTGTCGGAAAGTCCGGTGTTCCAGGCGATGAAGGAGGAGGGCGAGCTTGCCGGTAATCCCTTCGTCGAA
AGCTTCACCTACCCTGGCAACAAGCGCCGCATTTTCATCGCGCTGTTCGGCATCGCCGCCGGGCTAACCGTGATCTGGTA
CACGGCGATGTTCTCCGGCCTCAGCTTCCTCAAGTCTGCAATGCGCATGGAGGATACTCTGGCCGAGGTCGTCGTCGGTA
TCGGCGCCACACTCGGAATGGGCTTCTTCATCTACTTCGGCTCTCTTTCCGACCGTATCGGCCGTAAGAAGCCCATCATC
ATCGGCTATGCCGTCACGCTGCTAATGCTCTTTCCCACGTTCTGGCTGATGGGGGCCGCCGCCAATCCGCAACTCGCCGA
GGCGGCAGAGCGCAACCCGGTCGTCGTAGCCGGGCCTGACTGCAACTACAGCCCCTTTGCCTCTGAACAAGTCAGCAATT
GCGGCAAGCTCCTGGCTGACCTGGCGGCGTCCGGTGTGTCTTATAGTCTGCGCGATGACGCTGTGTTTGGCATGACCGCA
GGTGGTTCAGCGGTTGATCTTGCCAGCTATCCGTGGACGGACAAGGCTGCTGCGCGCGCCAAGGCGCTCCAGTCCGAGCT
TTCCGCGCATGGCTACGATTTCGCCAAGGTCCAGCCCTCGCTCGGCCGGATTGTCGCGGTTATCGGTGCGCTGCTGGCGC
TCATGGCGATGTCCGGTGCGACCTACGGGCCGGTGGCCGCCCTTCTTTCCGAGATGTTCCCGCCGCGCATCCGATACAGT
TCGATGTCGATCCCGTATCATCTCGGCACGGGCTACTTCGGGGGTTTCCTGCCGCTGATTTCCAGCTACATCGTCGCGCG
CACCGGCGATCCCTATGCCGGGCTATGGTACACTTGGGTGGTCGTCCTGGTCGCGCTCCTCGTTGCGGCGTGGGGTTTGC
GGCCAGGCCTGCCCGCCGACTTCACGGATGACTGA

Upstream 100 bases:

>100_bases
GGGATGCCGCGCTGTCCGCGCTGGCTTAGCGTTTTGCAGGGCTTGCTCCGCAAGTGGGCGCTTCGCTAGAGCAGCCGCAT
AAACAGGACGTATCGGGGGA

Downstream 100 bases:

>100_bases
CATTTCCCTTCCGACGACGCCCGACCTGCCCGAGTCCTCCCTTCGCCTGCGGATCGATGCCGATGCGCTTGCGTCTAACT
GGCGCGCACTCGACGCGATG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MCVNASVTKNDAAKPSASDIRLVIAASSAGTVFEWYDFFIYGTLASIIGRTFFPSDNATLQVLLVWAGFAVGFGFRPLGA
VLFGYLGDKLGRKYTFLVTVTLMGVATAGVGLIPSAATIGLAAPAIVILLRVLQGLALGGEYGGAAIYVAEHAPGGRRGY
YTSYIQASVVGGFVLSLIVVLSSKALMSDAVWNDWGWRVPFLVSLALLAISLWMRMKLSESPVFQAMKEEGELAGNPFVE
SFTYPGNKRRIFIALFGIAAGLTVIWYTAMFSGLSFLKSAMRMEDTLAEVVVGIGATLGMGFFIYFGSLSDRIGRKKPII
IGYAVTLLMLFPTFWLMGAAANPQLAEAAERNPVVVAGPDCNYSPFASEQVSNCGKLLADLAASGVSYSLRDDAVFGMTA
GGSAVDLASYPWTDKAAARAKALQSELSAHGYDFAKVQPSLGRIVAVIGALLALMAMSGATYGPVAALLSEMFPPRIRYS
SMSIPYHLGTGYFGGFLPLISSYIVARTGDPYAGLWYTWVVVLVALLVAAWGLRPGLPADFTDD

Sequences:

>Translated_544_residues
MCVNASVTKNDAAKPSASDIRLVIAASSAGTVFEWYDFFIYGTLASIIGRTFFPSDNATLQVLLVWAGFAVGFGFRPLGA
VLFGYLGDKLGRKYTFLVTVTLMGVATAGVGLIPSAATIGLAAPAIVILLRVLQGLALGGEYGGAAIYVAEHAPGGRRGY
YTSYIQASVVGGFVLSLIVVLSSKALMSDAVWNDWGWRVPFLVSLALLAISLWMRMKLSESPVFQAMKEEGELAGNPFVE
SFTYPGNKRRIFIALFGIAAGLTVIWYTAMFSGLSFLKSAMRMEDTLAEVVVGIGATLGMGFFIYFGSLSDRIGRKKPII
IGYAVTLLMLFPTFWLMGAAANPQLAEAAERNPVVVAGPDCNYSPFASEQVSNCGKLLADLAASGVSYSLRDDAVFGMTA
GGSAVDLASYPWTDKAAARAKALQSELSAHGYDFAKVQPSLGRIVAVIGALLALMAMSGATYGPVAALLSEMFPPRIRYS
SMSIPYHLGTGYFGGFLPLISSYIVARTGDPYAGLWYTWVVVLVALLVAAWGLRPGLPADFTDD
>Mature_544_residues
MCVNASVTKNDAAKPSASDIRLVIAASSAGTVFEWYDFFIYGTLASIIGRTFFPSDNATLQVLLVWAGFAVGFGFRPLGA
VLFGYLGDKLGRKYTFLVTVTLMGVATAGVGLIPSAATIGLAAPAIVILLRVLQGLALGGEYGGAAIYVAEHAPGGRRGY
YTSYIQASVVGGFVLSLIVVLSSKALMSDAVWNDWGWRVPFLVSLALLAISLWMRMKLSESPVFQAMKEEGELAGNPFVE
SFTYPGNKRRIFIALFGIAAGLTVIWYTAMFSGLSFLKSAMRMEDTLAEVVVGIGATLGMGFFIYFGSLSDRIGRKKPII
IGYAVTLLMLFPTFWLMGAAANPQLAEAAERNPVVVAGPDCNYSPFASEQVSNCGKLLADLAASGVSYSLRDDAVFGMTA
GGSAVDLASYPWTDKAAARAKALQSELSAHGYDFAKVQPSLGRIVAVIGALLALMAMSGATYGPVAALLSEMFPPRIRYS
SMSIPYHLGTGYFGGFLPLISSYIVARTGDPYAGLWYTWVVVLVALLVAAWGLRPGLPADFTDD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=341, Percent_Identity=34.0175953079179, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1788292, Length=335, Percent_Identity=37.3134328358209, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=321, Percent_Identity=34.8909657320872, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=32.5814536340852, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=313, Percent_Identity=23.961661341853, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082404, Length=310, Percent_Identity=25.1612903225806, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 57795; Mature: 57795

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVNASVTKNDAAKPSASDIRLVIAASSAGTVFEWYDFFIYGTLASIIGRTFFPSDNATL
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
QVLLVWAGFAVGFGFRPLGAVLFGYLGDKLGRKYTFLVTVTLMGVATAGVGLIPSAATIG
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHH
LAAPAIVILLRVLQGLALGGEYGGAAIYVAEHAPGGRRGYYTSYIQASVVGGFVLSLIVV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LSSKALMSDAVWNDWGWRVPFLVSLALLAISLWMRMKLSESPVFQAMKEEGELAGNPFVE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHH
SFTYPGNKRRIFIALFGIAAGLTVIWYTAMFSGLSFLKSAMRMEDTLAEVVVGIGATLGM
HCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFFIYFGSLSDRIGRKKPIIIGYAVTLLMLFPTFWLMGAAANPQLAEAAERNPVVVAGPD
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEECCC
CNYSPFASEQVSNCGKLLADLAASGVSYSLRDDAVFGMTAGGSAVDLASYPWTDKAAARA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCEEECCCCCCCCHHHHHH
KALQSELSAHGYDFAKVQPSLGRIVAVIGALLALMAMSGATYGPVAALLSEMFPPRIRYS
HHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEC
SMSIPYHLGTGYFGGFLPLISSYIVARTGDPYAGLWYTWVVVLVALLVAAWGLRPGLPAD
CCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
FTDD
CCCC
>Mature Secondary Structure
MCVNASVTKNDAAKPSASDIRLVIAASSAGTVFEWYDFFIYGTLASIIGRTFFPSDNATL
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
QVLLVWAGFAVGFGFRPLGAVLFGYLGDKLGRKYTFLVTVTLMGVATAGVGLIPSAATIG
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHH
LAAPAIVILLRVLQGLALGGEYGGAAIYVAEHAPGGRRGYYTSYIQASVVGGFVLSLIVV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LSSKALMSDAVWNDWGWRVPFLVSLALLAISLWMRMKLSESPVFQAMKEEGELAGNPFVE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHH
SFTYPGNKRRIFIALFGIAAGLTVIWYTAMFSGLSFLKSAMRMEDTLAEVVVGIGATLGM
HCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFFIYFGSLSDRIGRKKPIIIGYAVTLLMLFPTFWLMGAAANPQLAEAAERNPVVVAGPD
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEECCC
CNYSPFASEQVSNCGKLLADLAASGVSYSLRDDAVFGMTAGGSAVDLASYPWTDKAAARA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCEEECCCCCCCCHHHHHH
KALQSELSAHGYDFAKVQPSLGRIVAVIGALLALMAMSGATYGPVAALLSEMFPPRIRYS
HHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEC
SMSIPYHLGTGYFGGFLPLISSYIVARTGDPYAGLWYTWVVVLVALLVAAWGLRPGLPAD
CCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
FTDD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]