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The map label for this gene is proS

Identifier: 87199967

GI number: 87199967

Start: 2068026

End: 2069573

Strand: Reverse

Name: proS

Synonym: Saro_1950

Alternate gene names: 87199967

Gene position: 2069573-2068026 (Counterclockwise)

Preceding gene: 87199968

Following gene: 87199966

Centisome position: 58.11

GC content: 63.5

Gene sequence:

>1548_bases
ATGAACCAGCCCGCAATAAAGCACGCGCTCAACGTCAAGCGCGCCGACGACTTCGCCCAGTGGTACCAGGCCGTGATCGC
CGAGGCCGAGCTTGCCGAGGAATCCGGTGTGCGCGGCTGCATGGTCATCAAGCCTTGGGGTTATGGCATCTGGGAACGTA
TCCAGAAGCTTATGGATGCCGAGATCAAGGAGGCAGGCGTCGAGAACTGCTACTTCCCGCTGTTCATCCCGTTGTCCTAC
TTCACCAAGGAAGCGGAACACGTCGAAGGCTTTGCCAAGGAAATGGCGGTCGTCACGCACCACCGCCTTATTTCGGACGG
AAAGGGCGGCCTGACGCCCGACCCGGAAGCCAAGCTGGAAGAGCCGCTTGTGGTCCGTCCCACTTCCGAGACCGTTATCG
GCGCGGCGATGTCTCGCTGGATTCAGTCCTGGCGGGATCTTCCGCTGCTTACCAATCAGTGGGCCAACGTCGTGCGCTGG
GAAATGCGCACGCGCATGTTCCTCCGCACCAGCGAGTTCCTCTGGCAGGAAGGCCACACCGCGCACGTCGACGAGGCGGA
CGCGATGAAGGAAACCCTGCGCGCGCTCGAAATGTATCGTGCCTTTGCCGAAGGCCCGCTCGCCATGCCGGTGATCGCGG
GACCCAAGCCCGAGAACGAGCGTTTCCCCGGTGCGGTCGAGACCTTCTCGATCGAGGCCATGATGCAGGATGGCAAGGCC
CTCCAGGCCGGCACTTCGCACTACCTAGGCACGACCTTCGCCAAGGCGGCGGGCATCCAGTACCAGAACAAGGAAGGCCA
GCAGGCCCTCGCGCACACCACGTCGTGGGGCGTCTCCACTCGTCTGATCGGCGGCGTCATCATGACCCACGGAGACGACG
ATGGTCTGCGAGTGCCCCCTCAGGTCGCACCGCAGCAGATCGTCATCCTGCCGATGCTCCGCGATAACGAAGGCGATGAC
GCGCTGCTGGCCTATTGCGAGGAAATCCGCGCGTCTCTGGTAAAGCTGTCGGTGTTCGGGGAACGCATCCGCGTTCTCCT
CGACAAGCGCCCCGGCAAGGCCACGCAGAAGCGCTGGGCGTGGGTCAAGAAAGGCATGCCGCTCATTCTCGAGATCGGCG
GACGCGATGCCGAAGGCGGCCTGGTTTCCGTGCTGCGGCGGGATCGCCTGTGGCGCCAGGATGCCAAGCCTAACTTCGTT
GGCCAGGCCAAGGACGACTTCCTCGCCAGCGCCGCGACCGAACTCGAATCGATCCAGGCTGCACTCTATGATGAAGCCCG
TGCGCGCCGCGATGCGCAGATCGTGCGCGACGTGACCGACCTTGAAGGCCTCAAGGGATACTTTGCGGAGGGCAACAAGT
ACCCGGGATGGGTCGAGATGGGCTGGGCCAAGCCCACCGGCGAAGCGCTCGACAAGGTTGTCGAGCAACTCAAGGCGCTG
AAGCTGACCATCCGCAACACGCCGATGGACGCGGAAAAGCCTGTGGGCGCTTGCCCCTTCACCGGCGAGCCCGCGGTCGA
GAAGATCCTGATCGCGCGGTCCTACTGA

Upstream 100 bases:

>100_bases
CAGAAGAAGCTCGACCAGCTCTCGAAGTGATGCCCGTCTCCCGCAATCGCCCTACGCGGGGCATTGCGGGAAACGCCTTT
GGGCTTTAACGCGCCAGCCC

Downstream 100 bases:

>100_bases
TTTCCTTGAGGGTGGCGTGCTATCGCCACCCTTTTGGGCCCAGCGTCTTGGCGTGGCGTAGACGTGCGATATTGTCGCGC
GCCATGCGAACGATGCTTGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNQPAIKHALNVKRADDFAQWYQAVIAEAELAEESGVRGCMVIKPWGYGIWERIQKLMDAEIKEAGVENCYFPLFIPLSY
FTKEAEHVEGFAKEMAVVTHHRLISDGKGGLTPDPEAKLEEPLVVRPTSETVIGAAMSRWIQSWRDLPLLTNQWANVVRW
EMRTRMFLRTSEFLWQEGHTAHVDEADAMKETLRALEMYRAFAEGPLAMPVIAGPKPENERFPGAVETFSIEAMMQDGKA
LQAGTSHYLGTTFAKAAGIQYQNKEGQQALAHTTSWGVSTRLIGGVIMTHGDDDGLRVPPQVAPQQIVILPMLRDNEGDD
ALLAYCEEIRASLVKLSVFGERIRVLLDKRPGKATQKRWAWVKKGMPLILEIGGRDAEGGLVSVLRRDRLWRQDAKPNFV
GQAKDDFLASAATELESIQAALYDEARARRDAQIVRDVTDLEGLKGYFAEGNKYPGWVEMGWAKPTGEALDKVVEQLKAL
KLTIRNTPMDAEKPVGACPFTGEPAVEKILIARSY

Sequences:

>Translated_515_residues
MNQPAIKHALNVKRADDFAQWYQAVIAEAELAEESGVRGCMVIKPWGYGIWERIQKLMDAEIKEAGVENCYFPLFIPLSY
FTKEAEHVEGFAKEMAVVTHHRLISDGKGGLTPDPEAKLEEPLVVRPTSETVIGAAMSRWIQSWRDLPLLTNQWANVVRW
EMRTRMFLRTSEFLWQEGHTAHVDEADAMKETLRALEMYRAFAEGPLAMPVIAGPKPENERFPGAVETFSIEAMMQDGKA
LQAGTSHYLGTTFAKAAGIQYQNKEGQQALAHTTSWGVSTRLIGGVIMTHGDDDGLRVPPQVAPQQIVILPMLRDNEGDD
ALLAYCEEIRASLVKLSVFGERIRVLLDKRPGKATQKRWAWVKKGMPLILEIGGRDAEGGLVSVLRRDRLWRQDAKPNFV
GQAKDDFLASAATELESIQAALYDEARARRDAQIVRDVTDLEGLKGYFAEGNKYPGWVEMGWAKPTGEALDKVVEQLKAL
KLTIRNTPMDAEKPVGACPFTGEPAVEKILIARSY
>Mature_515_residues
MNQPAIKHALNVKRADDFAQWYQAVIAEAELAEESGVRGCMVIKPWGYGIWERIQKLMDAEIKEAGVENCYFPLFIPLSY
FTKEAEHVEGFAKEMAVVTHHRLISDGKGGLTPDPEAKLEEPLVVRPTSETVIGAAMSRWIQSWRDLPLLTNQWANVVRW
EMRTRMFLRTSEFLWQEGHTAHVDEADAMKETLRALEMYRAFAEGPLAMPVIAGPKPENERFPGAVETFSIEAMMQDGKA
LQAGTSHYLGTTFAKAAGIQYQNKEGQQALAHTTSWGVSTRLIGGVIMTHGDDDGLRVPPQVAPQQIVILPMLRDNEGDD
ALLAYCEEIRASLVKLSVFGERIRVLLDKRPGKATQKRWAWVKKGMPLILEIGGRDAEGGLVSVLRRDRLWRQDAKPNFV
GQAKDDFLASAATELESIQAALYDEARARRDAQIVRDVTDLEGLKGYFAEGNKYPGWVEMGWAKPTGEALDKVVEQLKAL
KLTIRNTPMDAEKPVGACPFTGEPAVEKILIARSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=523, Percent_Identity=36.9024856596558, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI71991342, Length=447, Percent_Identity=37.8076062639821, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI71991346, Length=372, Percent_Identity=40.8602150537634, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6321808, Length=433, Percent_Identity=38.1062355658199, Blast_Score=283, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24649466, Length=389, Percent_Identity=40.3598971722365, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24649468, Length=389, Percent_Identity=40.3598971722365, Blast_Score=283, Evalue=3e-76,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NOVAD (Q2G6Y3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497224.1
- ProteinModelPortal:   Q2G6Y3
- SMR:   Q2G6Y3
- STRING:   Q2G6Y3
- GeneID:   3917265
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1950
- NMPDR:   fig|48935.1.peg.2659
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   EGFAKEM
- PhylomeDB:   Q2G6Y3
- ProtClustDB:   PRK08661
- BioCyc:   NARO279238:SARO_1950-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 57474; Mature: 57474

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPAIKHALNVKRADDFAQWYQAVIAEAELAEESGVRGCMVIKPWGYGIWERIQKLMDA
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
EIKEAGVENCYFPLFIPLSYFTKEAEHVEGFAKEMAVVTHHRLISDGKGGLTPDPEAKLE
HHHHCCCCCCEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EPLVVRPTSETVIGAAMSRWIQSWRDLPLLTNQWANVVRWEMRTRMFLRTSEFLWQEGHT
CCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AHVDEADAMKETLRALEMYRAFAEGPLAMPVIAGPKPENERFPGAVETFSIEAMMQDGKA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCH
LQAGTSHYLGTTFAKAAGIQYQNKEGQQALAHTTSWGVSTRLIGGVIMTHGDDDGLRVPP
HHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCEEEECCCCCCCCCCC
QVAPQQIVILPMLRDNEGDDALLAYCEEIRASLVKLSVFGERIRVLLDKRPGKATQKRWA
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
WVKKGMPLILEIGGRDAEGGLVSVLRRDRLWRQDAKPNFVGQAKDDFLASAATELESIQA
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALYDEARARRDAQIVRDVTDLEGLKGYFAEGNKYPGWVEMGWAKPTGEALDKVVEQLKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
KLTIRNTPMDAEKPVGACPFTGEPAVEKILIARSY
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNQPAIKHALNVKRADDFAQWYQAVIAEAELAEESGVRGCMVIKPWGYGIWERIQKLMDA
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
EIKEAGVENCYFPLFIPLSYFTKEAEHVEGFAKEMAVVTHHRLISDGKGGLTPDPEAKLE
HHHHCCCCCCEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EPLVVRPTSETVIGAAMSRWIQSWRDLPLLTNQWANVVRWEMRTRMFLRTSEFLWQEGHT
CCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AHVDEADAMKETLRALEMYRAFAEGPLAMPVIAGPKPENERFPGAVETFSIEAMMQDGKA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCH
LQAGTSHYLGTTFAKAAGIQYQNKEGQQALAHTTSWGVSTRLIGGVIMTHGDDDGLRVPP
HHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCEEEECCCCCCCCCCC
QVAPQQIVILPMLRDNEGDDALLAYCEEIRASLVKLSVFGERIRVLLDKRPGKATQKRWA
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
WVKKGMPLILEIGGRDAEGGLVSVLRRDRLWRQDAKPNFVGQAKDDFLASAATELESIQA
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALYDEARARRDAQIVRDVTDLEGLKGYFAEGNKYPGWVEMGWAKPTGEALDKVVEQLKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
KLTIRNTPMDAEKPVGACPFTGEPAVEKILIARSY
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA