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The map label for this gene is trmFO

Identifier: 87199932

GI number: 87199932

Start: 2026593

End: 2027963

Strand: Reverse

Name: trmFO

Synonym: Saro_1915

Alternate gene names: 87199932

Gene position: 2027963-2026593 (Counterclockwise)

Preceding gene: 87199934

Following gene: 87199929

Centisome position: 56.94

GC content: 63.75

Gene sequence:

>1371_bases
ATGACGCATCAGGTACACATCATTGGCGGCGGCATGGCCGGAACGGAGGCCGCCTGGCAACTTGCGCGCCGGGGCATTCG
TGTGCGGCTGTCCGAAATGCGCGGAGGCGGCGATACTACTCCCGCCCACAACGGAGACGGGCTTGCCGAACTGGTCTGCT
CCAACTCGTTCCGGTCGGACGACGACGAAAAGAACGCGGTTGGCCTGCTGCACTACGAGATGAGGCAATGTGACAGCCTG
CTGATGGCAGCGGCGGCCAAGGCGCGCGTTCCGGCGGGGTCGGCGCTGGCGGTTGACCGGGATGTATTCTCGGCCGAAGT
CGAAGCCGCGTTGCGCGCGCAGCCGACGCTGGAGATCGTGCGCGAGCGGGTCGATGTGCTGCCGTCGGATGGCCTGACCA
TTGTGGCGACCGGCCCGCTCACCGCGCCGTCGCTGGCTAACAGCATCGGCAGTGCGACCGGAGCCGACAGTCTTGCCTTC
TTCGATGCCATCGCGCCGATCGTCTACCGCGACAGCATCGACATGGGCGTGGCATGGATGGCCTCGCGATGGGACAAGGG
CGCTGAAGCCTCGCTGGCGATGGGCGGTGACGGGCGCGATTACATCAACTGTCCAATGACCCGAGACCAGTACCTCGCCT
TCCGCGAAGAACTGCTCGCCGGCGAGAAGACAGAGTTCAAGGAGTGGGAGGCAAACACTCCCTACTTCGACGGCTGCATG
CCCATCGAGGTCATGGCCGCCCGCGGCGAAGAGACCTTGCGTTTCGGTCCGATGAAGCCTGTCGGGCTCGACAATCCCCA
TTGGGCAACCGCGGAGCATCCGAACGGGCGTTGGCCCTATGCGGTGGTCCAACTCCGCCAGGACAACAAGCTTGGCACGC
TGTGGAACATGGTCGGCTTCCAGACCAAGCTCAAGCATGCCGAACAAGTGCGCGTGTTCCGGACCATCCCGGGCCTCGAG
AATGCAGAGTTTGCCCGCCTCGGAGGCCTGCATCGCAACACCTTCCTCAATTCGCCGACGCTTCTGGATCGCCAATTGCG
CTTGAAGTCTGCTCCGAACGTTCGCTTCGCAGGGCAGATAACCGGATGCGAGGGCTACGTGGAGAGCGGCTCGGTCGGCA
TGCTTGCCGGTTTGATGGTTGCTGCACAGATCGCTGGGCTCGACTGGTCCCCTCCCCCGCGCACCACGGCGCTCGGCGCG
CTGCTGGCGCACATCACCGGCGATGCCGAAGCTGAGAGCTTCCAGCCGATGAACGTGAACTTCGGCCTGTTTTCACCGGT
AGACGCATCCGTGAAGAAGAAGGTTCGCAAGGAAGCATACACTGCTCGGGCGAAGGCCGATCTTTCGGACTGGATAGCCA
CGCTGGCGTAA

Upstream 100 bases:

>100_bases
AGGCCGTTTCTCTATCAGCGCACGTTTCGGCACTTGTGGATCTTCCCCCTGCTTTGACGACGGTATTGCCGCAGCGACCG
CGCGATGCCAAGGGCGGACC

Downstream 100 bases:

>100_bases
CTGGCGAGCGAAGGCGCGAATCTACCGGCAGGTGCATTTCTGTTTGGAACGTGGCTTGTCCTTGGGCCGCGTTGTCGCGA
ATTCCTGTCGCGTCAGGAAT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTHQVHIIGGGMAGTEAAWQLARRGIRVRLSEMRGGGDTTPAHNGDGLAELVCSNSFRSDDDEKNAVGLLHYEMRQCDSL
LMAAAAKARVPAGSALAVDRDVFSAEVEAALRAQPTLEIVRERVDVLPSDGLTIVATGPLTAPSLANSIGSATGADSLAF
FDAIAPIVYRDSIDMGVAWMASRWDKGAEASLAMGGDGRDYINCPMTRDQYLAFREELLAGEKTEFKEWEANTPYFDGCM
PIEVMAARGEETLRFGPMKPVGLDNPHWATAEHPNGRWPYAVVQLRQDNKLGTLWNMVGFQTKLKHAEQVRVFRTIPGLE
NAEFARLGGLHRNTFLNSPTLLDRQLRLKSAPNVRFAGQITGCEGYVESGSVGMLAGLMVAAQIAGLDWSPPPRTTALGA
LLAHITGDAEAESFQPMNVNFGLFSPVDASVKKKVRKEAYTARAKADLSDWIATLA

Sequences:

>Translated_456_residues
MTHQVHIIGGGMAGTEAAWQLARRGIRVRLSEMRGGGDTTPAHNGDGLAELVCSNSFRSDDDEKNAVGLLHYEMRQCDSL
LMAAAAKARVPAGSALAVDRDVFSAEVEAALRAQPTLEIVRERVDVLPSDGLTIVATGPLTAPSLANSIGSATGADSLAF
FDAIAPIVYRDSIDMGVAWMASRWDKGAEASLAMGGDGRDYINCPMTRDQYLAFREELLAGEKTEFKEWEANTPYFDGCM
PIEVMAARGEETLRFGPMKPVGLDNPHWATAEHPNGRWPYAVVQLRQDNKLGTLWNMVGFQTKLKHAEQVRVFRTIPGLE
NAEFARLGGLHRNTFLNSPTLLDRQLRLKSAPNVRFAGQITGCEGYVESGSVGMLAGLMVAAQIAGLDWSPPPRTTALGA
LLAHITGDAEAESFQPMNVNFGLFSPVDASVKKKVRKEAYTARAKADLSDWIATLA
>Mature_455_residues
THQVHIIGGGMAGTEAAWQLARRGIRVRLSEMRGGGDTTPAHNGDGLAELVCSNSFRSDDDEKNAVGLLHYEMRQCDSLL
MAAAAKARVPAGSALAVDRDVFSAEVEAALRAQPTLEIVRERVDVLPSDGLTIVATGPLTAPSLANSIGSATGADSLAFF
DAIAPIVYRDSIDMGVAWMASRWDKGAEASLAMGGDGRDYINCPMTRDQYLAFREELLAGEKTEFKEWEANTPYFDGCMP
IEVMAARGEETLRFGPMKPVGLDNPHWATAEHPNGRWPYAVVQLRQDNKLGTLWNMVGFQTKLKHAEQVRVFRTIPGLEN
AEFARLGGLHRNTFLNSPTLLDRQLRLKSAPNVRFAGQITGCEGYVESGSVGMLAGLMVAAQIAGLDWSPPPRTTALGAL
LAHITGDAEAESFQPMNVNFGLFSPVDASVKKKVRKEAYTARAKADLSDWIATLA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_NOVAD (Q2G718)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497189.1
- STRING:   Q2G718
- GeneID:   3917138
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1915
- NMPDR:   fig|48935.1.peg.886
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   NARO279238:SARO_1915-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49317; Mature: 49185

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHQVHIIGGGMAGTEAAWQLARRGIRVRLSEMRGGGDTTPAHNGDGLAELVCSNSFRSD
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCCCCHHHHHHCCCCCCC
DDEKNAVGLLHYEMRQCDSLLMAAAAKARVPAGSALAVDRDVFSAEVEAALRAQPTLEIV
CCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHH
RERVDVLPSDGLTIVATGPLTAPSLANSIGSATGADSLAFFDAIAPIVYRDSIDMGVAWM
HHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCHHHH
ASRWDKGAEASLAMGGDGRDYINCPMTRDQYLAFREELLAGEKTEFKEWEANTPYFDGCM
HHHCCCCCCCEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PIEVMAARGEETLRFGPMKPVGLDNPHWATAEHPNGRWPYAVVQLRQDNKLGTLWNMVGF
CHHHEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
QTKLKHAEQVRVFRTIPGLENAEFARLGGLHRNTFLNSPTLLDRQLRLKSAPNVRFAGQI
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEEE
TGCEGYVESGSVGMLAGLMVAAQIAGLDWSPPPRTTALGALLAHITGDAEAESFQPMNVN
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
FGLFSPVDASVKKKVRKEAYTARAKADLSDWIATLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
THQVHIIGGGMAGTEAAWQLARRGIRVRLSEMRGGGDTTPAHNGDGLAELVCSNSFRSD
CEEEEEEECCCCCHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCCCCHHHHHHCCCCCCC
DDEKNAVGLLHYEMRQCDSLLMAAAAKARVPAGSALAVDRDVFSAEVEAALRAQPTLEIV
CCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHH
RERVDVLPSDGLTIVATGPLTAPSLANSIGSATGADSLAFFDAIAPIVYRDSIDMGVAWM
HHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCHHHH
ASRWDKGAEASLAMGGDGRDYINCPMTRDQYLAFREELLAGEKTEFKEWEANTPYFDGCM
HHHCCCCCCCEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PIEVMAARGEETLRFGPMKPVGLDNPHWATAEHPNGRWPYAVVQLRQDNKLGTLWNMVGF
CHHHEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
QTKLKHAEQVRVFRTIPGLENAEFARLGGLHRNTFLNSPTLLDRQLRLKSAPNVRFAGQI
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEEE
TGCEGYVESGSVGMLAGLMVAAQIAGLDWSPPPRTTALGALLAHITGDAEAESFQPMNVN
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
FGLFSPVDASVKKKVRKEAYTARAKADLSDWIATLA
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA