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The map label for this gene is pycB [H]

Identifier: 87199876

GI number: 87199876

Start: 1959513

End: 1960985

Strand: Reverse

Name: pycB [H]

Synonym: Saro_1859

Alternate gene names: 87199876

Gene position: 1960985-1959513 (Counterclockwise)

Preceding gene: 87199877

Following gene: 87199875

Centisome position: 55.06

GC content: 66.67

Gene sequence:

>1473_bases
ATGACCGAGATCCGTCTCGTCGACGTCACCATGCGCGATGGCAACCAGAGCCTTTGGGGTGCCACCGGCCTCAACACCGC
GCACATGCTCCAGGCGGCGGCGCTGACCGAGGCCTGCGGCTTCCGTGCGATCGACTTCACCTCGTCGAGCCACATGGCCG
TGGCCGTGCGCTACTTCCAGAACAACCCGTGGGAACGCCTGCGCCGCATGGCCGCAGCCATGCCCACCACGCCGCTCCAG
TTCATCACCACGGGCCTGCGCTTCATCGCCTGGCAGCAGGCCGATCCAGAATTCATGCGCCTGGTATACCGCGCCTTGCA
GGCCAACGGCGTCGGCCGGTTCGTGCTACTCGATCCGATGCACGAAGTGCCCGCGGTGATCGAGGCTGCCAAGCTGGTGA
AGGAAGAAGGCAACGCCGAAGTAATGGCGGCGCTCACCTTCACTCTTTCCGAGATCCACACCGACCAGTTCTACGCCGAC
TTTGCCCGCGCCGTCGGGCAGAGCCCGAACATCGACCTGTTCTACATCAAGGACCCTGCCGGACTTCTCTCGGTAGACCG
CGCCCGCACGCTCGTGCCAGCGGTGAAGGCCGTGATCGGCAATCGCCCGCTGGAAATCCACGCCCACACCACTGTCGGCC
AGGGCATGATGGCGAGCCTCGAAGCGGCGAAGCTCGGCATTTCCGCCATCCATGTCGGCGTCGGACCGGGCGGTGACGGT
TCGTCGTTGCCCGAAGCCAACCGCATGGTCGCAAATCTCGAGGAGGCGGGCTTTTCGGTCGGCGTGGACAAGGCGGCGCT
GAAGCGCCTCACCACCTATTGGGAGCGCGTCGCGCTGGCGGAGGGGCTTCCGCCCGGCACTCCGCAGAACTTCGACGCCA
GCTTCCTGCGCCACCAGATCGCGGGCGGCGTGATGACCACCATCGCCCGCCAGCTCGACGAACTGGGCCTGTCCGACAGG
ATGGGCGCGGTGATCGAGGAGACCGGGCAGGTCCGCGCCGATCTGGGCTTCCCGATCATGGTCACGCCGTTCCCGCAGAT
GGTCATGACCCAGGCCCTGTTCAACGTGATCGGAGAGCGGCGCTATGCGCAGGTCTCGGACCAGGTGATCCGCTATTGCA
TGGGCAAGTTCGGCAAGCCCACGTCGCCCGTGGACCGCGAGGTGCTGGCAACGATCATGGACCGCCCCCGCTCGCGCGAG
CTTGAGGACGAGCCCATGTTCCCCGCCCTTGGCGACCTGCGCCGCCAGTTCGGCAGCCATCTGCCGGACGAGGAATTCCT
GCTGCGCGCGGTCATGCCCGGCGAACAGGTCGATGCAATGCTCGCCGCCGGCCCGTCGCGCGCCACCTACTCGCCACAGG
CCGCCCCCATCCTCTCGTTGCTGCGCCAGCTTGCCGCAAGGCCTGACGCCCGCGACATCGTGATCGAACGCGAAGGCTTC
CGCCTTGCCCTCCATGCCGGAGCATCCCTTTGA

Upstream 100 bases:

>100_bases
AAGGCCTGCCGACCACCGCACCGCTCCACCGCCGCATCGTCCTCGACCCGCGCTTCGTCGCCGGCGGGGTCGACACCGGC
TTCCTGACAGGACTTGCCCA

Downstream 100 bases:

>100_bases
CATTTTCCCCGGAATTCAAGGCCCGTCTCAAGGACGCGGCCGGTTTCATCTTCGACATGGACGGCACCATCGCGCTGGGC
GACGCCAAGAGCGGCGGCCA

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTEIRLVDVTMRDGNQSLWGATGLNTAHMLQAAALTEACGFRAIDFTSSSHMAVAVRYFQNNPWERLRRMAAAMPTTPLQ
FITTGLRFIAWQQADPEFMRLVYRALQANGVGRFVLLDPMHEVPAVIEAAKLVKEEGNAEVMAALTFTLSEIHTDQFYAD
FARAVGQSPNIDLFYIKDPAGLLSVDRARTLVPAVKAVIGNRPLEIHAHTTVGQGMMASLEAAKLGISAIHVGVGPGGDG
SSLPEANRMVANLEEAGFSVGVDKAALKRLTTYWERVALAEGLPPGTPQNFDASFLRHQIAGGVMTTIARQLDELGLSDR
MGAVIEETGQVRADLGFPIMVTPFPQMVMTQALFNVIGERRYAQVSDQVIRYCMGKFGKPTSPVDREVLATIMDRPRSRE
LEDEPMFPALGDLRRQFGSHLPDEEFLLRAVMPGEQVDAMLAAGPSRATYSPQAAPILSLLRQLAARPDARDIVIEREGF
RLALHAGASL

Sequences:

>Translated_490_residues
MTEIRLVDVTMRDGNQSLWGATGLNTAHMLQAAALTEACGFRAIDFTSSSHMAVAVRYFQNNPWERLRRMAAAMPTTPLQ
FITTGLRFIAWQQADPEFMRLVYRALQANGVGRFVLLDPMHEVPAVIEAAKLVKEEGNAEVMAALTFTLSEIHTDQFYAD
FARAVGQSPNIDLFYIKDPAGLLSVDRARTLVPAVKAVIGNRPLEIHAHTTVGQGMMASLEAAKLGISAIHVGVGPGGDG
SSLPEANRMVANLEEAGFSVGVDKAALKRLTTYWERVALAEGLPPGTPQNFDASFLRHQIAGGVMTTIARQLDELGLSDR
MGAVIEETGQVRADLGFPIMVTPFPQMVMTQALFNVIGERRYAQVSDQVIRYCMGKFGKPTSPVDREVLATIMDRPRSRE
LEDEPMFPALGDLRRQFGSHLPDEEFLLRAVMPGEQVDAMLAAGPSRATYSPQAAPILSLLRQLAARPDARDIVIEREGF
RLALHAGASL
>Mature_489_residues
TEIRLVDVTMRDGNQSLWGATGLNTAHMLQAAALTEACGFRAIDFTSSSHMAVAVRYFQNNPWERLRRMAAAMPTTPLQF
ITTGLRFIAWQQADPEFMRLVYRALQANGVGRFVLLDPMHEVPAVIEAAKLVKEEGNAEVMAALTFTLSEIHTDQFYADF
ARAVGQSPNIDLFYIKDPAGLLSVDRARTLVPAVKAVIGNRPLEIHAHTTVGQGMMASLEAAKLGISAIHVGVGPGGDGS
SLPEANRMVANLEEAGFSVGVDKAALKRLTTYWERVALAEGLPPGTPQNFDASFLRHQIAGGVMTTIARQLDELGLSDRM
GAVIEETGQVRADLGFPIMVTPFPQMVMTQALFNVIGERRYAQVSDQVIRYCMGKFGKPTSPVDREVLATIMDRPRSREL
EDEPMFPALGDLRRQFGSHLPDEEFLLRAVMPGEQVDAMLAAGPSRATYSPQAAPILSLLRQLAARPDARDIVIEREGFR
LALHAGASL

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=362, Percent_Identity=25.1381215469613, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI106049295, Length=362, Percent_Identity=25.1381215469613, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI106049292, Length=362, Percent_Identity=25.1381215469613, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17562816, Length=499, Percent_Identity=24.0480961923848, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321376, Length=437, Percent_Identity=25.1716247139588, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319695, Length=437, Percent_Identity=24.4851258581236, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652212, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652210, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652214, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921944, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652216, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363050, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652224, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652222, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652220, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652218, Length=398, Percent_Identity=26.1306532663317, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 53509; Mature: 53378

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIRLVDVTMRDGNQSLWGATGLNTAHMLQAAALTEACGFRAIDFTSSSHMAVAVRYFQ
CCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEHH
NNPWERLRRMAAAMPTTPLQFITTGLRFIAWQQADPEFMRLVYRALQANGVGRFVLLDPM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCEEEEECCH
HEVPAVIEAAKLVKEEGNAEVMAALTFTLSEIHTDQFYADFARAVGQSPNIDLFYIKDPA
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GLLSVDRARTLVPAVKAVIGNRPLEIHAHTTVGQGMMASLEAAKLGISAIHVGVGPGGDG
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCCC
SSLPEANRMVANLEEAGFSVGVDKAALKRLTTYWERVALAEGLPPGTPQNFDASFLRHQI
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AGGVMTTIARQLDELGLSDRMGAVIEETGQVRADLGFPIMVTPFPQMVMTQALFNVIGER
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCH
RYAQVSDQVIRYCMGKFGKPTSPVDREVLATIMDRPRSRELEDEPMFPALGDLRRQFGSH
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
LPDEEFLLRAVMPGEQVDAMLAAGPSRATYSPQAAPILSLLRQLAARPDARDIVIEREGF
CCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCC
RLALHAGASL
EEEEECCCCC
>Mature Secondary Structure 
TEIRLVDVTMRDGNQSLWGATGLNTAHMLQAAALTEACGFRAIDFTSSSHMAVAVRYFQ
CCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEHH
NNPWERLRRMAAAMPTTPLQFITTGLRFIAWQQADPEFMRLVYRALQANGVGRFVLLDPM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCEEEEECCH
HEVPAVIEAAKLVKEEGNAEVMAALTFTLSEIHTDQFYADFARAVGQSPNIDLFYIKDPA
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GLLSVDRARTLVPAVKAVIGNRPLEIHAHTTVGQGMMASLEAAKLGISAIHVGVGPGGDG
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCCC
SSLPEANRMVANLEEAGFSVGVDKAALKRLTTYWERVALAEGLPPGTPQNFDASFLRHQI
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AGGVMTTIARQLDELGLSDRMGAVIEETGQVRADLGFPIMVTPFPQMVMTQALFNVIGER
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCH
RYAQVSDQVIRYCMGKFGKPTSPVDREVLATIMDRPRSRELEDEPMFPALGDLRRQFGSH
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
LPDEEFLLRAVMPGEQVDAMLAAGPSRATYSPQAAPILSLLRQLAARPDARDIVIEREGF
CCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCC
RLALHAGASL
EEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]