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The map label for this gene is murE

Identifier: 87199150

GI number: 87199150

Start: 1172420

End: 1173868

Strand: Direct

Name: murE

Synonym: Saro_1128

Alternate gene names: 87199150

Gene position: 1172420-1173868 (Clockwise)

Preceding gene: 87199149

Following gene: 87199151

Centisome position: 32.92

GC content: 67.91

Gene sequence:

>1449_bases
ATGAAGCTCGGCGCGATCCTCGATGCGGCAGGCTATCCGGTGGCTGCGGCCGATGCCAACGTCACCGGCTTTGCCATCGA
TCATCGCAAGGTCGCCCCCGGCACCGTTTTCGGCGCGTTCCCCGGCACGCGCTTCAACGGCGAGGACTTCATCGCGGACG
CAGTGAAGGCAGGCGCGGTCGCTGTCGTCGCCCGACCCGAGGCTGTAGTGGAAGGCGCTGTCCACGTCGCCGATGCCGAG
CCGCGCCGGGCGTTCGCCGCGCTCGCCGCGCAGTTCTTCCAGCCGGTGCCGGAAACGGTCGTTGCCGTGACAGGAACCAA
CGGCAAGACTTCCACTGTCGAGATGACGCGGCAGATCTGGCGCATGGCTGGTCGTTCCGCGGCCTCGATCGGAACGCTTG
GCGTCACGACTGCGGACGAAAGCGTTTCCACCGGGCTGACCACGCCCGACATCGTCACGTTCCTGTCGAACATGACCGGC
CTTGCCCGCGAAGGGGTGACGCACGTCGCCTACGAAGCGTCGAGCCACGGCCTCTCGCAATTTCGCAACGAAGGCCTGCG
CGTGATCGCAGGGGCATTCACAAATCTCAGCCGCGACCATCTCGATTACCACCCGACGATGGAAGACTACTTCGCGGCCA
AGATGCGCCTGTTCGACCACGTCGTCGACGACGGTGGGGTGGCCGTCGTCTGGATGGACGATGAATGGTCGGGACGGGCA
GTGGAGCACGTCCGCCGACGGGGTCTGCGGCTGCTCTCGGTGGGCGAGCAGGGGGAGACGATCCGCCTGCTCGCCCGTAC
CCCCACCCAACTCGGTCAGGTGCTCGAAGTCGCGTTCGACGGTGCGCGGCGCAAGATCAGCCTGCCGCTGATCGGGGCCT
ATCAGGCCGCCAATGCGCTCGTTTCCGCGGGCCTCGCCATCGCTTCCGGCATCGACGCGGGGCAGGTCTTCGACGCGCTC
GCCCGGTTGCAGCCGGTGCGAGGAAGGCTGGAGCGCGCTGCGATCAATCGCGCCGGAGCGCCGGTCTATGTCGATTACGC
GCATACGCCCGACGCCATCGAGGCGGCCATCGCCGCGCTTCGCCCACACGTGAAGGGACGGCTGATCACCGTATTCGGTG
CCGGGGGCGACCGCGACGAGGGCAAGCGTCCCGAGATGGGCCGCGCGGCCTGCGCGGGTTCGGACCTTGTGATTGTCACG
GACGACAATCCGCGCGGCGAGGACCCGGCTGCGATCAGGGCCGCGATCATGGCGGGCTGCGCCGACAACGCGCGCGAGAT
CGGGGATCGGCGCAGCGCGATTGCGGCGGCAATTGCCGAGGCCGGGCGCGACGATATCGTCCTTGTCGCGGGGAAGGGGC
ACGAACAGGGGCAGATCATCGGCAGGGGCGATGCCATGCGCGTCCTGCCGTTCGACGACGTGCAGGTCGCAAGGGAGTGC
ACGACATGA

Upstream 100 bases:

>100_bases
TCGTGCCCCGCATCGGCCCGCTGCTCGGCGTCGTGCCCGACGACAGTCGCGACATCGACATTTCCGATCTTTCGCCCCTC
GTCGGCAACGGAGAAGGCGA

Downstream 100 bases:

>100_bases
CAGCCCATCCCGCCCTCATCGCCTGGCCCGAGGACCCGTCCGATGCGGCGGGTCTCGCACTTTGGACGAGCGAGGAAATC
GCTGCTGCGACCGGCGGGCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKLGAILDAAGYPVAAADANVTGFAIDHRKVAPGTVFGAFPGTRFNGEDFIADAVKAGAVAVVARPEAVVEGAVHVADAE
PRRAFAALAAQFFQPVPETVVAVTGTNGKTSTVEMTRQIWRMAGRSAASIGTLGVTTADESVSTGLTTPDIVTFLSNMTG
LAREGVTHVAYEASSHGLSQFRNEGLRVIAGAFTNLSRDHLDYHPTMEDYFAAKMRLFDHVVDDGGVAVVWMDDEWSGRA
VEHVRRRGLRLLSVGEQGETIRLLARTPTQLGQVLEVAFDGARRKISLPLIGAYQAANALVSAGLAIASGIDAGQVFDAL
ARLQPVRGRLERAAINRAGAPVYVDYAHTPDAIEAAIAALRPHVKGRLITVFGAGGDRDEGKRPEMGRAACAGSDLVIVT
DDNPRGEDPAAIRAAIMAGCADNAREIGDRRSAIAAAIAEAGRDDIVLVAGKGHEQGQIIGRGDAMRVLPFDDVQVAREC
TT

Sequences:

>Translated_482_residues
MKLGAILDAAGYPVAAADANVTGFAIDHRKVAPGTVFGAFPGTRFNGEDFIADAVKAGAVAVVARPEAVVEGAVHVADAE
PRRAFAALAAQFFQPVPETVVAVTGTNGKTSTVEMTRQIWRMAGRSAASIGTLGVTTADESVSTGLTTPDIVTFLSNMTG
LAREGVTHVAYEASSHGLSQFRNEGLRVIAGAFTNLSRDHLDYHPTMEDYFAAKMRLFDHVVDDGGVAVVWMDDEWSGRA
VEHVRRRGLRLLSVGEQGETIRLLARTPTQLGQVLEVAFDGARRKISLPLIGAYQAANALVSAGLAIASGIDAGQVFDAL
ARLQPVRGRLERAAINRAGAPVYVDYAHTPDAIEAAIAALRPHVKGRLITVFGAGGDRDEGKRPEMGRAACAGSDLVIVT
DDNPRGEDPAAIRAAIMAGCADNAREIGDRRSAIAAAIAEAGRDDIVLVAGKGHEQGQIIGRGDAMRVLPFDDVQVAREC
TT
>Mature_482_residues
MKLGAILDAAGYPVAAADANVTGFAIDHRKVAPGTVFGAFPGTRFNGEDFIADAVKAGAVAVVARPEAVVEGAVHVADAE
PRRAFAALAAQFFQPVPETVVAVTGTNGKTSTVEMTRQIWRMAGRSAASIGTLGVTTADESVSTGLTTPDIVTFLSNMTG
LAREGVTHVAYEASSHGLSQFRNEGLRVIAGAFTNLSRDHLDYHPTMEDYFAAKMRLFDHVVDDGGVAVVWMDDEWSGRA
VEHVRRRGLRLLSVGEQGETIRLLARTPTQLGQVLEVAFDGARRKISLPLIGAYQAANALVSAGLAIASGIDAGQVFDAL
ARLQPVRGRLERAAINRAGAPVYVDYAHTPDAIEAAIAALRPHVKGRLITVFGAGGDRDEGKRPEMGRAACAGSDLVIVT
DDNPRGEDPAAIRAAIMAGCADNAREIGDRRSAIAAAIAEAGRDDIVLVAGKGHEQGQIIGRGDAMRVLPFDDVQVAREC
TT

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=472, Percent_Identity=36.864406779661, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786274, Length=135, Percent_Identity=37.7777777777778, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50690; Mature: 50690

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGAILDAAGYPVAAADANVTGFAIDHRKVAPGTVFGAFPGTRFNGEDFIADAVKAGAV
CCCCCEECCCCCCEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCE
AVVARPEAVVEGAVHVADAEPRRAFAALAAQFFQPVPETVVAVTGTNGKTSTVEMTRQIW
EEEECCHHHHHCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
RMAGRSAASIGTLGVTTADESVSTGLTTPDIVTFLSNMTGLAREGVTHVAYEASSHGLSQ
HHHCCCCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCHHHHHH
FRNEGLRVIAGAFTNLSRDHLDYHPTMEDYFAAKMRLFDHVVDDGGVAVVWMDDEWSGRA
HHHCCCEEEEHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
VEHVRRRGLRLLSVGEQGETIRLLARTPTQLGQVLEVAFDGARRKISLPLIGAYQAANAL
HHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCEEEHHHHHHHH
VSAGLAIASGIDAGQVFDALARLQPVRGRLERAAINRAGAPVYVDYAHTPDAIEAAIAAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
RPHVKGRLITVFGAGGDRDEGKRPEMGRAACAGSDLVIVTDDNPRGEDPAAIRAAIMAGC
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHC
ADNAREIGDRRSAIAAAIAEAGRDDIVLVAGKGHEQGQIIGRGDAMRVLPFDDVQVAREC
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEEECCCHHHHHHHC
TT
CC
>Mature Secondary Structure
MKLGAILDAAGYPVAAADANVTGFAIDHRKVAPGTVFGAFPGTRFNGEDFIADAVKAGAV
CCCCCEECCCCCCEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCE
AVVARPEAVVEGAVHVADAEPRRAFAALAAQFFQPVPETVVAVTGTNGKTSTVEMTRQIW
EEEECCHHHHHCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
RMAGRSAASIGTLGVTTADESVSTGLTTPDIVTFLSNMTGLAREGVTHVAYEASSHGLSQ
HHHCCCCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCHHHHHH
FRNEGLRVIAGAFTNLSRDHLDYHPTMEDYFAAKMRLFDHVVDDGGVAVVWMDDEWSGRA
HHHCCCEEEEHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
VEHVRRRGLRLLSVGEQGETIRLLARTPTQLGQVLEVAFDGARRKISLPLIGAYQAANAL
HHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCEEEHHHHHHHH
VSAGLAIASGIDAGQVFDALARLQPVRGRLERAAINRAGAPVYVDYAHTPDAIEAAIAAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
RPHVKGRLITVFGAGGDRDEGKRPEMGRAACAGSDLVIVTDDNPRGEDPAAIRAAIMAGC
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHC
ADNAREIGDRRSAIAAAIAEAGRDDIVLVAGKGHEQGQIIGRGDAMRVLPFDDVQVAREC
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEEECCCHHHHHHHC
TT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA