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The map label for this gene is murF [H]

Identifier: 87199151

GI number: 87199151

Start: 1173865

End: 1175334

Strand: Direct

Name: murF [H]

Synonym: Saro_1129

Alternate gene names: 87199151

Gene position: 1173865-1175334 (Clockwise)

Preceding gene: 87199150

Following gene: 87199152

Centisome position: 32.96

GC content: 67.82

Gene sequence:

>1470_bases
ATGACAGCCCATCCCGCCCTCATCGCCTGGCCCGAGGACCCGTCCGATGCGGCGGGTCTCGCACTTTGGACGAGCGAGGA
AATCGCTGCTGCGACCGGCGGGCGGGCAAGCGCGCCCTTTGCCGTCTCGGGCGTCGAGATCGACAGCCGCGAGGTCGTCG
AGGGAGATCTTTTCTTCGCGCTGAAGGGCGAAAATTCCGACGGGCATCGCTACCTTGAAGGCGCCTTCGGTCGAGGTGCG
GCAGGCGCTGTCGTGGAGCATGCCTGTTTCGGGCCGCACGTCCTTGTCTCGAACACTTCAACCGCGCTTGAGGATCTTGG
AAAAGCCGCGCGAAATCGGGTGGATGCGGGCATTATCGGAGTCACCGGCTCAGCAGGAAAGACCGGCGTCAAGGAAGCCC
TCTATGCCGCGCTGGACCGTGCGAGCCGGGGGCGCGCGCACCGTTCCGTCAAGAGCTACAACAATCACGTGGGCGTTCCG
CTGAGCCTTGCCCGGATGCCTGCGCGTACAAGCTTCGGCGTGTTCGAGATGGGCATGAACCACGCGGGCGAACTGGCGGC
ACTGACCCGCTTGGTGCGGCCGCACGTCGCCATCGTCACGACCATCGCCCCGGCGCACATCGGCCACTTCGCCGGCGAGG
AAGCCATTGCCGATGCCAAGGCCGAGATCTTCGAAGGGCTGGAGCCCGGCGGTGTCGCGATCATCCCGGCGGACAACCGC
CATTTCGAGCACCTTCGCGCCAAGGCCACGCTGCACGCCGAGCGCGTCATAAGCTTCGGCGTCGCCAAGGACGCGACGGT
GCGGCTGCTCGATGCCGTGCCCGCACCGGGTGGCGGCACCCTCGTCACGGCTGACCTGGGCGGACGCAAGGTCTGCTACA
CGATCGCGCAACCGGGCGAACACTGGGTGGCCAATTCGCTGGCGGTCATGGCTGCGGTCGAGGCGATCGGCGGCGATCTG
GGCGCGGCCGGTCTTGCGCTGGCGGAAATGGAAGGCCTTGCCGGACGCGGCGCGCGCCATGCCATTCCGGCGCATGGCGG
CGACGGATCGGGCAGCGCGCTCCTGATCGACGAAAGCTACAACGCCAATCCCGCGTCGATGGCGGTGACGTTGCGTCAGC
TTGGCCAGACGCCGGCCGCGCGCCGCGTCGCCATTCTCGGGGCCATGCGCGAGCTTGGCGCCGACGAGGCGCGCTACCAC
GCGGAGCTTGCCGGCCCGGTCGCGGAAGGCAAAGTCGACAGACTGATCCTGGTGGGCGGCGAAATGACCGCGCTGGCCGA
CGCATTGGGGAAAAGCGGCTCGGCCGCGCTTGCCGGTGCAATTGATGTGGCGCATGTGCAAACGGCGGCGGAAGCGGCGG
AATGCCTGGCTGCAGATGGCGTGCGCGGGGGTGACGCCATCCTCGTCAAGGGGTCGAATTCGGTCGGGCTGGGTGCGCTG
GTGCGCTCGCTCACCGGCAAGGAACGTTGA

Upstream 100 bases:

>100_bases
TCGCGGGGAAGGGGCACGAACAGGGGCAGATCATCGGCAGGGGCGATGCCATGCGCGTCCTGCCGTTCGACGACGTGCAG
GTCGCAAGGGAGTGCACGAC

Downstream 100 bases:

>100_bases
ATGCTCTACCTGCTGGCGCAATGGCTGCACTTTGAGGGGTTCAGCAACCTCATCCGATACCAGACGTTCCGCGCCGGCGC
GACGCTGCTGACCGCGCTGG

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAHPALIAWPEDPSDAAGLALWTSEEIAAATGGRASAPFAVSGVEIDSREVVEGDLFFALKGENSDGHRYLEGAFGRGA
AGAVVEHACFGPHVLVSNTSTALEDLGKAARNRVDAGIIGVTGSAGKTGVKEALYAALDRASRGRAHRSVKSYNNHVGVP
LSLARMPARTSFGVFEMGMNHAGELAALTRLVRPHVAIVTTIAPAHIGHFAGEEAIADAKAEIFEGLEPGGVAIIPADNR
HFEHLRAKATLHAERVISFGVAKDATVRLLDAVPAPGGGTLVTADLGGRKVCYTIAQPGEHWVANSLAVMAAVEAIGGDL
GAAGLALAEMEGLAGRGARHAIPAHGGDGSGSALLIDESYNANPASMAVTLRQLGQTPAARRVAILGAMRELGADEARYH
AELAGPVAEGKVDRLILVGGEMTALADALGKSGSAALAGAIDVAHVQTAAEAAECLAADGVRGGDAILVKGSNSVGLGAL
VRSLTGKER

Sequences:

>Translated_489_residues
MTAHPALIAWPEDPSDAAGLALWTSEEIAAATGGRASAPFAVSGVEIDSREVVEGDLFFALKGENSDGHRYLEGAFGRGA
AGAVVEHACFGPHVLVSNTSTALEDLGKAARNRVDAGIIGVTGSAGKTGVKEALYAALDRASRGRAHRSVKSYNNHVGVP
LSLARMPARTSFGVFEMGMNHAGELAALTRLVRPHVAIVTTIAPAHIGHFAGEEAIADAKAEIFEGLEPGGVAIIPADNR
HFEHLRAKATLHAERVISFGVAKDATVRLLDAVPAPGGGTLVTADLGGRKVCYTIAQPGEHWVANSLAVMAAVEAIGGDL
GAAGLALAEMEGLAGRGARHAIPAHGGDGSGSALLIDESYNANPASMAVTLRQLGQTPAARRVAILGAMRELGADEARYH
AELAGPVAEGKVDRLILVGGEMTALADALGKSGSAALAGAIDVAHVQTAAEAAECLAADGVRGGDAILVKGSNSVGLGAL
VRSLTGKER
>Mature_488_residues
TAHPALIAWPEDPSDAAGLALWTSEEIAAATGGRASAPFAVSGVEIDSREVVEGDLFFALKGENSDGHRYLEGAFGRGAA
GAVVEHACFGPHVLVSNTSTALEDLGKAARNRVDAGIIGVTGSAGKTGVKEALYAALDRASRGRAHRSVKSYNNHVGVPL
SLARMPARTSFGVFEMGMNHAGELAALTRLVRPHVAIVTTIAPAHIGHFAGEEAIADAKAEIFEGLEPGGVAIIPADNRH
FEHLRAKATLHAERVISFGVAKDATVRLLDAVPAPGGGTLVTADLGGRKVCYTIAQPGEHWVANSLAVMAAVEAIGGDLG
AAGLALAEMEGLAGRGARHAIPAHGGDGSGSALLIDESYNANPASMAVTLRQLGQTPAARRVAILGAMRELGADEARYHA
ELAGPVAEGKVDRLILVGGEMTALADALGKSGSAALAGAIDVAHVQTAAEAAECLAADGVRGGDAILVKGSNSVGLGALV
RSLTGKER

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=449, Percent_Identity=30.9576837416481, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49705; Mature: 49574

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHPALIAWPEDPSDAAGLALWTSEEIAAATGGRASAPFAVSGVEIDSREVVEGDLFFA
CCCCCCEEECCCCCCCCCCEEEEECCHHHHCCCCCCCCCEEEECEEECCCCEECCEEEEE
LKGENSDGHRYLEGAFGRGAAGAVVEHACFGPHVLVSNTSTALEDLGKAARNRVDAGIIG
EECCCCCCCEEHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEE
VTGSAGKTGVKEALYAALDRASRGRAHRSVKSYNNHVGVPLSLARMPARTSFGVFEMGMN
ECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHCCC
HAGELAALTRLVRPHVAIVTTIAPAHIGHFAGEEAIADAKAEIFEGLEPGGVAIIPADNR
CCHHHHHHHHHHCCCEEEEEEECHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
HFEHLRAKATLHAERVISFGVAKDATVRLLDAVPAPGGGTLVTADLGGRKVCYTIAQPGE
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCC
HWVANSLAVMAAVEAIGGDLGAAGLALAEMEGLAGRGARHAIPAHGGDGSGSALLIDESY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCC
NANPASMAVTLRQLGQTPAARRVAILGAMRELGADEARYHAELAGPVAEGKVDRLILVGG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECC
EMTALADALGKSGSAALAGAIDVAHVQTAAEAAECLAADGVRGGDAILVKGSNSVGLGAL
CHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
VRSLTGKER
HHHHCCCCC
>Mature Secondary Structure 
TAHPALIAWPEDPSDAAGLALWTSEEIAAATGGRASAPFAVSGVEIDSREVVEGDLFFA
CCCCCEEECCCCCCCCCCEEEEECCHHHHCCCCCCCCCEEEECEEECCCCEECCEEEEE
LKGENSDGHRYLEGAFGRGAAGAVVEHACFGPHVLVSNTSTALEDLGKAARNRVDAGIIG
EECCCCCCCEEHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEE
VTGSAGKTGVKEALYAALDRASRGRAHRSVKSYNNHVGVPLSLARMPARTSFGVFEMGMN
ECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHCCC
HAGELAALTRLVRPHVAIVTTIAPAHIGHFAGEEAIADAKAEIFEGLEPGGVAIIPADNR
CCHHHHHHHHHHCCCEEEEEEECHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
HFEHLRAKATLHAERVISFGVAKDATVRLLDAVPAPGGGTLVTADLGGRKVCYTIAQPGE
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCC
HWVANSLAVMAAVEAIGGDLGAAGLALAEMEGLAGRGARHAIPAHGGDGSGSALLIDESY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCC
NANPASMAVTLRQLGQTPAARRVAILGAMRELGADEARYHAELAGPVAEGKVDRLILVGG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECC
EMTALADALGKSGSAALAGAIDVAHVQTAAEAAECLAADGVRGGDAILVKGSNSVGLGAL
CHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
VRSLTGKER
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893; 9274032 [H]