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The map label for this gene is amtB [H]

Identifier: 87198718

GI number: 87198718

Start: 733046

End: 734404

Strand: Direct

Name: amtB [H]

Synonym: Saro_0694

Alternate gene names: 87198718

Gene position: 733046-734404 (Clockwise)

Preceding gene: 87198717

Following gene: 87198719

Centisome position: 20.58

GC content: 63.58

Gene sequence:

>1359_bases
TTGAGCGTGCCTTCCATTTCCGACCTTAGGGGAACCAACATGATCCGCAAGATGATCGGTGGCTTGGCGGGAACGGGCGC
TTCGCTCTTCGCTGCCACGGCTGCTCACGCGCAGGAGGCCGCAGCGGCCACTGTCGACAAGGGCGATACCGCCTGGATGC
TGACTTCGACAATTCTCGTGCTGATGATGATCCTGCCGGGCCTGGCGCTGTTCTACGGCGGCCTGACCCGGACCAAGAAC
ATGCTCTCCACGATGACCCAGATCGGCGCCGTCGCGTGCTTCGCGATGCTGCTGTGGGTCATGTACGGCTATGGCCTTGC
ATTCGGCCCCGAAGGCAACGCCTTCATCAGTTGGGGCAAGTGGTTCCTTGCGGGCGTTACCCCGGCAAGCCAGGCCGCGA
CCTTCACTGCGGGTGTCGAAATCCCGGAATACGTGTTCATCTGCTTCCAGATGACGTTTGCGGCGATCACAATCGCGCTG
GTTCTCGGCTCGGTCGTCGAACGCATGAAGTTTTCGGCGGTGATGGCGTTCGCGGCCGTGTGGCTGACCATCGTCTACTT
CCCGATCGCACACATGGTCTGGGCGGCGGGCGGCCTGTTCTTCGACATGGGCGCTCTGGATTTCGCCGGCGGTACGGTCG
TTCACATCAACGCTGGCGTCTCGGCACTGGTGGCCTGCCTTATCCTCGGCAAGCGCATCGGTTTCCAGAAGGACATCATG
GCGCCTCACTCGCTGACCATGACCGGCATCGGCACCGGCCTGCTGTGGGTGGGTTGGTTCGGCTTCAACGCCGGTTCGGC
GCTTGAAGCAAACGGCTCGGCTGCGCTTGCCATGATCAACACTTTCGTCGCCACCGCTTCGGCCGGTCTGTTCTGGATGC
TTGCCGAGCGTCTTTCGGGCCACAAGGGTTCGGCACTCGGCTTCTGCTCGGGCATCATCGCCGGCCTCGTCGCCGTGACG
CCTGCCGCCGGCAACTCTGGTCCTTTCGGCGCGATCGTTCTCGGTGGCCTCGCCTCGGTCGTCTGCTTCTACATGGTCAC
CGTGGTGAAGCCCAAGCTTGGCTATGACGACGCTCTCGATGCTTTCGGCATCCATGGCGTCGGCGGCATGATCGGCGCGG
TCGGTACCGCAGTCGTCTATGCGCCCTCGCTGGGTGGTCCGGGCGCCGCCGACTACGACATGGGCGCCAAGCTCGTCGTC
CAGATCATGGCGGTCGGCACCACCATCGTCTGGGCCGCGATTGGCACGGTCGTCGCCATGTACGTCGCCAAGGCTCTGAC
CGGCCTGCGGGTCACCGAAGAAGTCGAACGCGAAGGCCTCGACCTCGGTGAGCATGGCGAGCGCGCCTACAACTACTGA

Upstream 100 bases:

>100_bases
AGGCCGCCAGCACCGAGGCGATTGGCGACGGCAAGGTGTTCGTCCTCGATCTCGCGTCCGCGACCCGTATCCGCACCGGC
GAAACCGGGGACACCGCGCT

Downstream 100 bases:

>100_bases
TTAACCGAATTGGCGGGGTGGGGGAGACAGATCCTCTCCCTCTCATCTCCCACCCCGCCAAGCCTTGTTCCTCCTGCGAA
CAGACTGAAGGCCGGAAGCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSVPSISDLRGTNMIRKMIGGLAGTGASLFAATAAHAQEAAAATVDKGDTAWMLTSTILVLMMILPGLALFYGGLTRTKN
MLSTMTQIGAVACFAMLLWVMYGYGLAFGPEGNAFISWGKWFLAGVTPASQAATFTAGVEIPEYVFICFQMTFAAITIAL
VLGSVVERMKFSAVMAFAAVWLTIVYFPIAHMVWAAGGLFFDMGALDFAGGTVVHINAGVSALVACLILGKRIGFQKDIM
APHSLTMTGIGTGLLWVGWFGFNAGSALEANGSAALAMINTFVATASAGLFWMLAERLSGHKGSALGFCSGIIAGLVAVT
PAAGNSGPFGAIVLGGLASVVCFYMVTVVKPKLGYDDALDAFGIHGVGGMIGAVGTAVVYAPSLGGPGAADYDMGAKLVV
QIMAVGTTIVWAAIGTVVAMYVAKALTGLRVTEEVEREGLDLGEHGERAYNY

Sequences:

>Translated_452_residues
MSVPSISDLRGTNMIRKMIGGLAGTGASLFAATAAHAQEAAAATVDKGDTAWMLTSTILVLMMILPGLALFYGGLTRTKN
MLSTMTQIGAVACFAMLLWVMYGYGLAFGPEGNAFISWGKWFLAGVTPASQAATFTAGVEIPEYVFICFQMTFAAITIAL
VLGSVVERMKFSAVMAFAAVWLTIVYFPIAHMVWAAGGLFFDMGALDFAGGTVVHINAGVSALVACLILGKRIGFQKDIM
APHSLTMTGIGTGLLWVGWFGFNAGSALEANGSAALAMINTFVATASAGLFWMLAERLSGHKGSALGFCSGIIAGLVAVT
PAAGNSGPFGAIVLGGLASVVCFYMVTVVKPKLGYDDALDAFGIHGVGGMIGAVGTAVVYAPSLGGPGAADYDMGAKLVV
QIMAVGTTIVWAAIGTVVAMYVAKALTGLRVTEEVEREGLDLGEHGERAYNY
>Mature_451_residues
SVPSISDLRGTNMIRKMIGGLAGTGASLFAATAAHAQEAAAATVDKGDTAWMLTSTILVLMMILPGLALFYGGLTRTKNM
LSTMTQIGAVACFAMLLWVMYGYGLAFGPEGNAFISWGKWFLAGVTPASQAATFTAGVEIPEYVFICFQMTFAAITIALV
LGSVVERMKFSAVMAFAAVWLTIVYFPIAHMVWAAGGLFFDMGALDFAGGTVVHINAGVSALVACLILGKRIGFQKDIMA
PHSLTMTGIGTGLLWVGWFGFNAGSALEANGSAALAMINTFVATASAGLFWMLAERLSGHKGSALGFCSGIIAGLVAVTP
AAGNSGPFGAIVLGGLASVVCFYMVTVVKPKLGYDDALDAFGIHGVGGMIGAVGTAVVYAPSLGGPGAADYDMGAKLVVQ
IMAVGTTIVWAAIGTVVAMYVAKALTGLRVTEEVEREGLDLGEHGERAYNY

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=414, Percent_Identity=49.7584541062802, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32564042, Length=454, Percent_Identity=28.6343612334802, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=27.9816513761468, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=439, Percent_Identity=27.7904328018223, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=435, Percent_Identity=26.8965517241379, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=34.4339622641509, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324187, Length=427, Percent_Identity=35.3629976580796, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321559, Length=424, Percent_Identity=32.7830188679245, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI161078314, Length=428, Percent_Identity=26.8691588785047, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46681; Mature: 46550

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPSISDLRGTNMIRKMIGGLAGTGASLFAATAAHAQEAAAATVDKGDTAWMLTSTILV
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LMMILPGLALFYGGLTRTKNMLSTMTQIGAVACFAMLLWVMYGYGLAFGPEGNAFISWGK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEECH
WFLAGVTPASQAATFTAGVEIPEYVFICFQMTFAAITIALVLGSVVERMKFSAVMAFAAV
HHHHCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLTIVYFPIAHMVWAAGGLFFDMGALDFAGGTVVHINAGVSALVACLILGKRIGFQKDIM
HHHHHHHHHHHHHHHCCCHHEECCCHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHCC
APHSLTMTGIGTGLLWVGWFGFNAGSALEANGSAALAMINTFVATASAGLFWMLAERLSG
CCCCEEEHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
HKGSALGFCSGIIAGLVAVTPAAGNSGPFGAIVLGGLASVVCFYMVTVVKPKLGYDDALD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AFGIHGVGGMIGAVGTAVVYAPSLGGPGAADYDMGAKLVVQIMAVGTTIVWAAIGTVVAM
HCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YVAKALTGLRVTEEVEREGLDLGEHGERAYNY
HHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVPSISDLRGTNMIRKMIGGLAGTGASLFAATAAHAQEAAAATVDKGDTAWMLTSTILV
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LMMILPGLALFYGGLTRTKNMLSTMTQIGAVACFAMLLWVMYGYGLAFGPEGNAFISWGK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEECH
WFLAGVTPASQAATFTAGVEIPEYVFICFQMTFAAITIALVLGSVVERMKFSAVMAFAAV
HHHHCCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLTIVYFPIAHMVWAAGGLFFDMGALDFAGGTVVHINAGVSALVACLILGKRIGFQKDIM
HHHHHHHHHHHHHHHCCCHHEECCCHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHCC
APHSLTMTGIGTGLLWVGWFGFNAGSALEANGSAALAMINTFVATASAGLFWMLAERLSG
CCCCEEEHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
HKGSALGFCSGIIAGLVAVTPAAGNSGPFGAIVLGGLASVVCFYMVTVVKPKLGYDDALD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AFGIHGVGGMIGAVGTAVVYAPSLGGPGAADYDMGAKLVVQIMAVGTTIVWAAIGTVVAM
HCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YVAKALTGLRVTEEVEREGLDLGEHGERAYNY
HHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]